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排序方式: 共有343条查询结果,搜索用时 15 毫秒
1.
Acute Immobilization Stress and Intraventricular Injection of CRF Suppress Naloxone-Induced LH Release in Ovariectomized Estrogen-Primed Rats 总被引:1,自引:0,他引:1
Tatsuo Akema Atsuhiko Chiba Reiji Shinozaki Morihiro Oshida Fukuko Kimura & Jun-ichi Toyoda 《Journal of neuroendocrinology》1996,8(8):647-652
The present study was undertaken to evaluate the role and possible interaction of the endogenous opioid peptide (EOP) and corticotropin-releasing factor (CRF) in the acute stress-induced suppression of gonadotropin secretion in ovariectomized estrogen-primed rats. An intravenous (i.v.) injection of naloxone (10 or 20 mg/kg), an EOP antagonist, significantly elevated serum luteinizing hormone (LH) levels within 10 min in non-stressed animals. The naloxone-induced LH release was completely eliminated when tested 30 min after the onset of acute immobilization. In a subsequent study, it was found that suppression of the naloxone-induced LH release occurred as early as 5 min after the stress onset, and was still evident 60 min after the end of a 30-min period of immobilization. The effect of naloxone was restored 3 h after liberation of the animal from the 30-min immobilization. An intraventricular (i.c.v.) injection of CRF (1 or 5 μg) also significantly suppressed, in a dose-related manner, the effect of a subsequent i.v. injection of naloxone. However, an i.c.v. injection of α -helical CRF(9-41) (25 or 50 μg), a CRF antagonist, prior to immobilization, could not interfere with the suppressive effect of stress on naloxone-induced LH release. These results suggest that both acute immobilization stress and CRF can inhibit the LH secretory activity without mediation by EOP neurons. However, the stress-related suppression may involve non-CRF mechanism(s). 相似文献
2.
Takashi Ito Ryuji Sakakibara MD Tatsuya Yamamoto Tomoyuki Uchiyama Zhi Liu Masato Asahina Morihiro Higashi Kimihito Arai Shoichi Ito Yusuke Awa Kaori Yamamoto Mika Kinou Tomonori Yamanishi Takamichi Hattori 《Clinical autonomic research》2006,16(1):66-71
Abstract Uro-neurological assessment was performed in four patients with small-fiber neuropathy due to amyloidosis (2 transthyretin-type/2
immunoglobulin light-chain-type). Voiding difficulties were due to detrusor weakness and impaired bladder sensation. In two
patients cholinesterase inhibition treatment caused urge incontinence, indicating detrusor denervation supersensitivity. The
underlying mechanisms of urinary dysfunction seem to involve postganglionic cholinergic and afferent somatic nerves. 相似文献
3.
Use of laboratory data to identify risk factors of giant coronary aneurysms due to Kawasaki disease 总被引:5,自引:0,他引:5
Yosikazu Nakamura Mayumi Yashiro Ritei Uehara Makoto Watanabe Morihiro Tajimi Izumi Oki Toshiyuki Ojima Tomoyoshi Sonobe Hiroshi Yanagawa 《Pediatrics international》2004,46(1):33-38
BACKGROUND: Although some laboratory findings are known to be indicators of the risk of giant coronary aneurysm formation among Kawasaki disease patients, an appropriate cut-off point to predict aneurysm formation is not clear. METHODS: One hundred and five patients with giant coronary aneurysms were selected from the 15th and 16th nationwide surveys of Kawasaki disease in Japan. A total of 2936 patients without Kawasaki disease were recruited from a single hospital as a control group. Odds ratios were calculated for six laboratory data with specific values as cut-off points. Receiver operating characteristic (ROC) curves were observed to determine the most appropriate laboratory tests and cut-off points. RESULTS: Hematocrit, leukocyte count, neutrophil proportion, and hemoglobin had one or more peaks of odds ratio for specific cut-off points, but they did not have a clear cut-off point for the predictor according to the receiver operating characteristic curves. Alanine aminotransferase (ALT) increased the risk of giant coronary aneurysms continuously so no clearly appropriate cut-off point was identified. Serum sodium concentration of 135 mEq/L had a peak of odds ratio, and those with <135 mEq/L had the highest odds ratio (4.78). This value seemed appropriate with a sensitivity of 78% and specificity of 57%, although the predictive positive value was as small as 5%. CONCLUSION: The author's propose that a serum sodium concentration of <135 mEq/L at the patient's first visit to hospital may be a predictor of giant coronary aneurysms due to Kawasaki disease. 相似文献
4.
Yoshiro Tanizaki Hikaru Kitani Morihiro Okazaki Takashi Mifune Fumihiro Mitsunobu Ikuro Kimura 《The Journal of asthma》1993,30(4):309-318
The effects of long-term glucocorticoid therapy on airway inflammation were examined in 84 asthma patients. The proportion of lymphocytes in bronchoalveolar lavage (BAL) fluid was significantly decreased in patients with steroid-dependent intractable asthma (SDIA) compared to results in non-SDIA patients, while BAL neutrophils were significantly increased in SDIA patients compared to results in non-SDIA patients. Regarding age, in patients under the age of 69 (except those between 30 and 39), BAL lymphocyte number was significantly decreased in SDIA compared with non-SDIA subjects, and in patients between 50 and 69, BAL neutrophils were significantly increased in SDIA compared with non-SDIA subjects. The number of BAL lymphocytes was significantly lower in patients with serum cortisol levels of less than 5.0 μg/dl than in those with levels of more than 5.1 μg/dl. BAL lymphocyte number was also significantly lower in patients who had received glucocorticoid therapy for more than 6 years than in those who had received such therapy for 2 years. These results show that long-term glucocorticoid therapy decreases the number of lymphocytes and increases neutrophil numbers in the airways. 相似文献
5.
Cloning and characterization of the cDNA encoding the HA protein of a hemagglutination-defective measles virus strain 总被引:3,自引:0,他引:3
Hiroyuki Saito Hiroyasu Sato Mariko Abe Seizaburo Harata Ken-Ichi Amano Tsunehisa Suto Morihiro Morita 《Virus genes》1994,8(1):107-113
cDNA clones corresponding to the mRNA for the hemagglutinin of the hemagglutination-defective strain AK-1 of measles virus were isolated and characterized. Compared with the prototype Edmonstron strain, 60 nucleotide substitutions that resulted in 18 amino acid changes were detected. An additional potential N-linked glycosylation site was added by point mutation, which was supported by the observation that the hemagglutinin of the AK-1 strain was stained more heavily after NaDodSO4PAGE and periodic acid-Schiff (PAS) staining than the Edmonston strain. Computer-assisted analysis revealed that three reverse turns in the secondary structure had disappeared in the hemagglutinin of the AK-1 strain. Moreover, one of these structural changes occurred in the closely glycosylated region at amino acid residues 168–240, which appeared to be a biologically important functional domain. The isoelectric point calculated from the predicted amino acid sequence became about 1 pH unit more basic in the AK-1 strain than the Edmonston strain. This present study is the first sequence analysis of the hemagglutinin gene in a hemagglutination-defective strain of the measles virus. 相似文献
6.
Y. Nose M. Nakamura T. Inoue O. Nakagaki Y. Watanabe M. Yokota T. Tajimi A. Kuroiwa J. Hattori M. Miura 《Medical & biological engineering & computing》1980,18(6):727-730
The reliability of the Japanese public telephone facilities to transmit electrocardiograms (e.c.g.) for computer interpretation
was assessed. The International Business Machine's (IBM) e.c.g. computer program by Bonner was used. No appreciable distortion
of e.c.g. was observed following repeated transmission from hospitals separated by 1000 km. Thirty-four normal and 66 abnormal
e.c.g.s. were transmitted twice. Identical results were observed in 97% of normals and 92% of abnormals.
Following these fundamental experiments, 1236 patients' e.c.g.s. were transmitted for computer intepretation. The study showed
that 98·6% (1219 cases) were technically satisfactory and 1·4% (17 cases) were not. The 17 unsatisfactory cases were classified
into ten unreceivable data formats, six inconsistent measurements and one unacceptable noise level.
The authors concluded that the Japanese public telephone facilities were acceptable for the transmission of e.c.g.s. for computer
interpretation. 相似文献
7.
Komada H Ito M Nishio M Kawano M Ohta H Tsurudome M Kusagawa S O'Brien M Bando H Ito Y 《Medical microbiology and immunology》2000,189(1):1-6
cDNAs encoding human parainfluenza virus type 4B (hPIV-4B) hemagglutinin neuraminidase (HN) protein were cloned and the nucleotide
sequences were determined. A high degree of identity (81.4%) was observed between the nucleotide sequences of hPIV-4A and
-4B HN proteins, and an 87.3% identity was found between the deduced amino acid sequences. This degree of identity is considered
to be greater than immunological similarity between hPIV-4A and -4B HN proteins determined using monoclonal antibodies. To
elucidate the causes of the antigenic difference between HN proteins of hPIV-4A and -4B, we constructed three cDNAs of hPIV-4B
HN whose potential N-glycosylation sites were partially or completely the same as in hPIV-4A HN cDNA. We compared the antigenicity of the expressed
wild-type and mutant proteins, and found that the antigenicities of the mutant hPIV-4B HN proteins were more similar to the
hPIV-4A HN protein than to the non-mutant hPIV-4B HN protein. This study indicated that the antigenic diversity between hPIV-4A
and -4B was partly caused by deletion or creation of glycosylation sites, showing that the point mutations resulting in deletion
or creation of glycosylation sites is one of the initial steps leading to the division of virus into subtypes.
Received: 21 January 2000 相似文献
8.
Kinugasa Eriko Igawa Ken Shimada Hisaki Kondo Morihiro Funakoshi Satoshi Imada Naoki Itami Noritomo Fukazawa Naoki Takubo Ryoko Kawata Yuichi Murota Hiroyuki 《Clinical and experimental nephrology》2021,25(8):875-884
Clinical and Experimental Nephrology - The pathophysiology of uremic pruritus (UP), which is characterized by systemic and intractable itching, remains unclear. As interleukin (IL)-31 may be... 相似文献
9.
Recombinant Sendai viruses with L1618V mutation in their L polymerase protein establish persistent infection, but not temperature sensitivity 总被引:2,自引:0,他引:2
The Sendai virus pi strain (SeVpi) isolated from cells persistently infected with SeV shows mainly two phenotypes: (1) temperature sensitivity and (2) an ability of establishing persistent infection (steady state). Three amino acid substitutions are found in the Lpi protein and are located at aa 1088, 1618, and 1664. Recombinant SeV(Lpi) (rSeV(Lpi)) having all these substitutions is temperature sensitive and is capable of establishing persistent infection (steady state). rSeVs carrying the fragment containing L1618V show both phenotypes. rSeV(L1618V), in which leucine at aa 1618 is replaced with valine, has the ability of establishing persistent infection, but is not a temperature-sensitive mutant, indicating that the ability of a virus to establish persistent infection can be separated from temperature sensitivity. The amino acid change at 1618(L-->V) coexisting with aa 1169 threonine is required for acquirement of a temperature-sensitive phenotype. Three amino acid substitutions are also found in the Ppi protein, but rSeV(Ppi) does not show these phenotypes. 相似文献
10.
Youichi Kumagai Tetsuhiko Tachikawa Morihiro Higashi Jun Sobajima Akemi Takahashi Kunihiko Amano Minoru Fukuchi Kei-ichiro Ishibashi Erito Mochiki Koji Yakabi Jun-ichi Tamaru Hideyuki Ishida 《Esophagus》2018,15(1):19-26