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1.
目的:探讨中医药治疗颞下颌关节紊乱病临床诊治的经验体会。方法:采用中医药方法对我科8年来收治的79例颞下颌关节紊乱病病例进行治疗并观察疗效。结果:治愈73例,显效4例,有效1例,无效1例,总有效率98.7%。结论:颞下颌关节紊乱病的病因复杂,应结合病因,采用有针对性的中西医综合治疗。  相似文献   
2.
在我国肺结核病人中复发涂阳病人约占20%,为尽快治愈这部分病人,使病人恢复健康,减少疾病的传播流行具有重要的意义。  相似文献   
3.
肿瘤坏死因子凋亡相关配体基因的克隆和表达   总被引:1,自引:1,他引:0  
苏海川  赵玉红  张惠中  范清宇 《医学争鸣》2003,24(16):1482-1484
目的:克隆、表达和鉴定肿瘤坏死因子凋亡相关配体(TRAIL).方法:从培养的人外周血淋巴细胞中提取总RNA,经RT-PCR获得TRAIL基因.将该基因克隆到pGEM-Teasy载体中,测序鉴定.将TRAIL基因插入pBV220表达载体中,42℃诱导表达4~5h,进行SDS-PAGE分析.免疫印迹法鉴定TRAIL蛋白的表达.结果:DNA测序证明,获得了TRAIL基因,其序列与GenBank中报道序列完全一致.SDSPAGE分析表明,TRAIL蛋白获得高效表达,分子质量为17ku,表达量约占菌体总蛋白的300k,.免疫印迹法鉴定显示,该蛋白可与鼠抗人TRAIL mAb产生阳性反应.结论:成功克隆和表达了TRAIL基因.  相似文献   
4.
目的 :探讨海港职工血压水平及其影响因素的人群分布 ,为心血管病防治提供科学依据。方法 :采用国际标准化的心血管病流行病学调查方法 ,调查 10 2 5 6名 18~ 5 4岁青岛港职工 ,男 7795例 ,女 2 46 1例。结果 :理想血压和正常血压男性为 5 8.6 % ,女性为 85 .2 %。 45~ 5 4岁职工中血压处在正常高值及高血压Ⅰ、Ⅱ、Ⅲ级水平的比例男性占 5 4.8% ,女性占 33 .2 %。经相关分析调整年龄后体重指数、腰臀围比值、腰围、心率与收缩压、舒张压均呈显著正相关 ,男性饮酒量与血压显著正相关。结论 :青岛港职工高血压患病率男性明显高于女性  相似文献   
5.
Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.  相似文献   
6.
Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.  相似文献   
7.
为了减少脑卒中的发病,降低其死亡率,提高患者的生活质量,笔者自1974年开始对脑卒中患者发病的有关因素及死亡原因进行分析、研究。同时回顾了1974~2004年我院收治的10547例脑卒中病例资料进行对比性研究。  相似文献   
8.
Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.  相似文献   
9.
Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.  相似文献   
10.
目的 分析益心汤用于心肾阳虚型慢性心力衰竭治疗的效果,为相关工作提供参考。方法 择取2019年2月15日至2021年2月15日前来上海市长宁区天山中区院就诊的200例慢性心力衰竭患者为研究对象。根据干预方式的不同,分为观察组、对照组,每组100例。对照组接受常规西医治疗,观察组患者应用益心汤治疗,比较2组治疗结果。结果 干预前2组受试者左心室的射血分数(LVEF)、左室舒张末期内径(LVEPD)以及左室收缩末期内径(LVESD)指标差异无统计学意义(P>0.05);干预后观察组患者的上述指标改善情况比对照组高(P<0.05)。观察组治疗效果比对照组高(P<0.05)。2组干预前各项血流动力学指标差异无统计学意义(P>0.05);干预后观察组二尖瓣环舒张早期与心房收缩期峰值运动速度(E/A)、心输出量(CO)以及心脏每搏输出量(SV)指标改善情况更好(P<0.05)。2组病患不良反应发生率差异无统计学意义(P>0.05)。结论 针对心肾阳虚型慢性心力衰竭患者来讲,使用益心汤治疗,能改善患者的心功能,且安全性强,值得临床中广。  相似文献   
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