全文获取类型
收费全文 | 674篇 |
免费 | 21篇 |
国内免费 | 9篇 |
专业分类
耳鼻咽喉 | 9篇 |
儿科学 | 7篇 |
基础医学 | 52篇 |
口腔科学 | 19篇 |
临床医学 | 31篇 |
内科学 | 247篇 |
皮肤病学 | 9篇 |
神经病学 | 29篇 |
特种医学 | 13篇 |
外科学 | 137篇 |
综合类 | 3篇 |
预防医学 | 3篇 |
药学 | 35篇 |
中国医学 | 1篇 |
肿瘤学 | 109篇 |
出版年
2023年 | 10篇 |
2022年 | 9篇 |
2021年 | 21篇 |
2020年 | 12篇 |
2019年 | 10篇 |
2018年 | 24篇 |
2017年 | 14篇 |
2016年 | 14篇 |
2015年 | 24篇 |
2014年 | 7篇 |
2013年 | 30篇 |
2012年 | 38篇 |
2011年 | 45篇 |
2010年 | 27篇 |
2009年 | 15篇 |
2008年 | 27篇 |
2007年 | 44篇 |
2006年 | 49篇 |
2005年 | 34篇 |
2004年 | 24篇 |
2003年 | 31篇 |
2002年 | 24篇 |
2001年 | 10篇 |
2000年 | 15篇 |
1999年 | 13篇 |
1998年 | 8篇 |
1997年 | 10篇 |
1996年 | 9篇 |
1995年 | 5篇 |
1994年 | 5篇 |
1993年 | 6篇 |
1992年 | 8篇 |
1991年 | 13篇 |
1990年 | 15篇 |
1989年 | 11篇 |
1988年 | 9篇 |
1987年 | 8篇 |
1986年 | 3篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1982年 | 3篇 |
1977年 | 2篇 |
1976年 | 3篇 |
1973年 | 1篇 |
1972年 | 2篇 |
1971年 | 1篇 |
1969年 | 2篇 |
1968年 | 1篇 |
1967年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有704条查询结果,搜索用时 15 毫秒
1.
2.
Y Kitamura M Watanabe S Komatsubara Y Sakata 《Hinyokika kiyo. Acta urologica Japonica》1990,36(5):535-539
Urinary excretion of glycine.prolile dipeptidile aminopeptidase (GP-DAP), N-acetyl-beta-D-glucosaminidase (NAG), alanine aminopeptidase (AAP) and beta 2-microglobulin (beta 2-M), alpha 1-microglobulin (alpha 1-M) was studied preoperatively in 32 patients with renal cell carcinoma. The excretion indices of GP-DAP, AAP and NAG were significantly higher than those in the healthy control group. The excretion of these enzymes obviously reflected the degree of the tumor progression. However, positive rates were not remarkable (37% for GP-DAP, 37% for AAP and 28% for NAG). The excretion of beta 2-M and alpha 1-M was not increased in renal cell carcinoma patients. 相似文献
3.
Expression of the PD-1 antigen on the surface of stimulated mouse T and B lymphocytes 总被引:28,自引:4,他引:28
Agata Yasutoshi; Kawasaki Akemi; Nishimura Hiroyuki; Ishida Yasumasa; Tsubat Takeshi; Yagita Hideo; Honjo Tasuku 《International immunology》1996,8(5):765-772
A mAb J43 has been produced against the product of the mousePD-1 gene, a member of the Ig gene superfamily, which was previouslyisolated from an apoptosis-induced T cell hybridoma (2B4.11)by using subtractive hybridization. Analyses by flow cytometryand immunoprecipitation using the J43 mAb revealed that thePD-1 gene product is a 50–55 kDa membrane protein expressedon the cell surface of several PD-1 cDNA transfectants and 2B4.11cells. Since the molecular weight calculated from the aminoacid sequence is 29,310, the PD-1 protein appears to be heavilyglycosylated. Normal murine lymphoid tissues such as thymus,spleen, lymph node and bone marrow contained very small numbersof PD-1+ cells. However, a significant PD-1+ population appearedin the thymocytes as well as T cells in spleen and lymph nodesby the in vivo anti-CD3 mAb treatment. Furthermore, the PD-1antigen expression was strongly induced in distinct subsetsof thymocytes and spleen T cells by in vitro stimulation witheither anti-CD3 mAb or concanavalin A (Con A) which could leadT cells to both activation and cell death. Similarly, PD-1 expressionwas induced on spleen B cells by in vitro stimulation with anti-IgMantibody. By contrast, PD-1 was not significantly expressedon lymphocytes by treatment with growth factor deprivation,dexamethasone or lipopolysaccharide. These results suggest thatthe expression of the PD-1 antigen is tightly regulated andinduced by signal transduction through the antigen receptorand do not exclude the possibility that the PD-1 antigen mayplay a role in clonal selection of lymphocytes although PD-1expression is not required for the common pathway of apoptosis. 相似文献
4.
5.
6.
Akitaka Yamasaki Kumiko Maruyama-Takahashi Kento Nishida Shogo Okazaki Kouki Okita Yasutoshi Akiyama Hideaki Suzuki Yuichi Endo Kazue Masuko Takashi Masuko Yoshihisa Tomioka 《Genes to cells : devoted to molecular & cellular mechanisms》2023,28(5):374-382
Human epidermal growth factor receptor (HER) family proteins are currently major targets of therapeutic monoclonal antibodies against various epithelial cancers. However, the resistance of cancer cells to HER family-targeted therapies, which may be caused by cancer heterogeneity and persistent HER phosphorylation, often reduces overall therapeutic effects. We herein showed that a newly discovered molecular complex between CD98 and HER2 affected HER function and cancer cell growth. The immunoprecipitation of the HER2 or HER3 protein from lysates of SKBR3 breast cancer (BrCa) cells revealed the HER2-CD98 or HER3-CD98 complex. The knockdown of CD98 by small interfering RNAs inhibited the phosphorylation of HER2 in SKBR3 cells. A bispecific antibody (BsAb) that recognized the HER2 and CD98 proteins was constructed from a humanized anti-HER2 (SER4) IgG and an anti-CD98 (HBJ127) single chain variable fragment, and this BsAb significantly inhibited the cell growth of SKBR3 cells. Prior to the inhibition of AKT phosphorylation, BsAb inhibited the phosphorylation of HER2, however, significant inhibition of HER2 phosphorylation was not observed in anti-HER2 pertuzumab, trastuzumab, SER4 or anti-CD98 HBJ127 in SKBR3 cells. The dual targeting of HER2 and CD98 has potential as a new therapeutic strategy for BrCa. 相似文献
7.
Kohno J Asai Y Nishio M Sakurai M Kawano K Hiramatsu H Kameda N Kishi N Okuda T Komatsubara S 《The Journal of antibiotics》1999,52(12):1114-1123
Four new antibiotics, TMC-171A (2), B (3), C (4) and TMC-154 (5) have been isolated from the fermentation of fungal strains Gliocladium sp. TC 1304 and TC 1282, respectively. Spectroscopic and degradation studies have shown that TMC-171s and TMC-154 were new members of the TMC-151 class of antibiotics, unique polyketides modified with a D-mannose and a D-mannitol or a D-arabitol. These compounds showed moderate cytotoxicity to various tumor cell lines. 相似文献
8.
Toshio Tsuyuguchi Tadahiro Takada Yoshifumi Kawarada Yuji Nimura Keita Wada Masato Nagino Toshihiko Mayumi Masahiro Yoshida Fumihiko Miura Atsushi Tanaka Yuichi Yamashita Masahiko Hirota Koichi Hirata Hideki Yasuda Yasutoshi Kimura Horst Neuhaus Steven Strasberg Henry Pitt Jacques Belghiti Giulio Belli John A. Windsor Miin-Fu Chen Sun-Whe Kim Christos Dervenis 《Journal of hepato-biliary-pancreatic sciences》2007,14(1):46-51
The principal management of acute cholecystitis is early cholecystectomy. However, percutaneous transhepatic gallbladder drainage (PTGBD) may be preferable for patients with moderate (grade II) or severe (grade III) acute cholecystitis. For patients with moderate (grade II) disease, PTGBD should be applied only when they do not respond to conservative treatment. For patients with severe (grade III) disease, PTGBD is recommended with intensive care. Percutaneous transhepatic gallbladder aspiration (PTGBA) is a simple alternative drainage method with fewer complications; however, its clinical usefulness has been shown only by case-series studies. To clarify the clinical value of these drainage methods, proper randomized trials should be done. This article describes techniques of drainage for acute cholecystitis. 相似文献
9.
Makoto Nishio Atsushi Horiike Hiroshi Nokihara Hidehito Horinouchi Shinji Nakamichi Hiroshi Wakui Fumiyoshi Ohyanagi Keita Kudo Noriko Yanagitani Shunji Takahashi Yasutoshi Kuboki Noboru Yamamoto Yasuhide Yamada Masaichi Abe Takashi Tahata Tomohide Tamura 《Investigational new drugs》2015,33(3):632-640