应用计算机信息技术创新护理管理模式

随着人类健康对护理要求越来越高,护理分工更加精细复杂,对护理管理提出了更高的要求,应用计算机网络技术科学地与医疗信息平台结合,节约成本,提高工作效率,使复杂的管理工作变得更系统、规范、便捷,是对护理管理模式的一次创新.     

Hepatoprotective effects of phloridzin on hepatic fibrosis induced by carbon tetrachloride against oxidative stress-triggered damage and fibrosis in rats

The present study was to study the hepatoprotective effects of phloridzin (PHL) on hepatic fibrosis induced by carbon tetrachloride (CCl?) in rats, on the basis of this investigation, the possible mechanism of PHL was elucidated. Male Sprague Dawley (SD) rats were randomly divided into six groups: control, model, PHL-L, PHL-M, PHL-H and colchine. All rats except control group were intraperitoneally injected with CCl?, and control rats were injected with olive oil, twice a week for eight weeks. At the same time, the rats were orally given homologue drugs once a day, respectively. Hepatoprotective effects of PHL were evaluated by liver weight indexes, biochemical values, total antioxidant capacity and total-superoxide dismutase, histopathological observations, hepatic fibrosis, and the hepatic fibrosis relative gene and protein expressions. PHL significantly improved hepatic function; remarkably decreased serum hyaluronic acid (HA), transforming growth factor-β1 (TGF-β1), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and liver tissues hydroxyproline, malondialdehyde (MDA) levels, increased glutathione peroxidase (GSH-Px), total-antioxygen capacity (T-AOC) and total-superoxide dismutase (T-SOD) contents of liver tissues; Real-time polymerase chain reaction (PCR) and immunohisto-chemical results showed PHL might markedly reverse the up-regulated mRNA and protein expressions of the α-smooth muscle actin (SMA), TGF-β1 and tissue inhibitor of metalloproteinase-1 (TIMP1), up-regulate the matrix metalloproteinase-1 (MMP1) mRNA and protein expressions. Histopathological observations provided supportive evidence for biochemical analyses and the hepatic fibrosis relative gene and protein expressions, and with the dose of PHL increasing, the aforesaid improvement became more and more strong. The studies demonstrated that PHL exerted beneficially hepatoprotective effects on hepatic fibrosis induced by CCl?, mainly enhancing antioxidant capacity of liver organizations, reduce the level of lipid peroxidation induced by CCl?, and protect hepatocyte membranes from damage, and alleviate hepatic fibrosis.     

坤泰胶囊联合激素替代治疗用于卵巢早衰疗效及安全性的系统评价

目的:基于荟萃分析(Meta分析)方法,系统评价坤泰胶囊联合激素替代治疗(hormone replacement therapy,HRT)用于卵巢早衰的疗效及安全性。方法:检索中国期刊全文数据库、维普数据库、万方数据库、中国生物医学文献数据库和PubMed数据库,检索时限为建库至2018年12月31日,收集坤泰胶囊联合HRT用于卵巢早衰的随机对照临床研究文献(研究组患者使用坤泰胶囊联合HRT;对照组患者单纯使用HRT),采用“偏倚风险评估工具”进行质量评价,运用RevMan 5.3软件对患者治疗前后的卵泡刺激素(FSH)、黄体生成素(LH)和雌二醇(E2)水平,症状积分,总有效率和不良反应发生情况进行Meta分析。结果:最终纳入15篇文献,涉及1343例患者。Meta分析结果显示,与单纯使用HRT比较,坤泰胶囊联合HRT能明显降低卵巢早衰患者的FSH水平(MD=-7.13,95%CI=-8.08^-6.19,P<0.00001)、LH水平(MD=-5.43,95%CI=-6.99^-3.88,P<0.00001),明显提高E2水平(SMD=1.14,95%CI=0.73~1.54,P<0.00001),明显降低症状积分(MD=-4.42,95%CI=-5.55^-3.28,P<0.00001),明显提高治疗总有效率(OR=3.53,95%CI=2.53~4.94,P<0.00001)和安全性(OR=0.56,95%CI=0.31~1.01,P=0.05),差异均有统计学意义。结论:坤泰胶囊联合HRT有明显优势,能在一定程度上改善卵巢早衰患者的临床症状,减少不良反应,提高安全性,可以作为较好的辅助搭配治疗。     

应用计算机信息技术创新护理管理模式

随着人类健康对护理要求越来越高,护理分工更加精细复杂,对护理管理提出了更高的要求,应用计算机网络技术科学地与医疗信息平台结合,节约成本,提高工作效率,使复杂的管理工作变得更系统、规范、便捷,是对护理管理模式的一次创新.     

Trametenolic Acid B Reverses Multidrug Resistance in Breast Cancer Cells Through Regulating the Expression Level of P‐Glycoprotein

Trametenolic acid B (TAB) is the main active composition of Trametes lactinea (Berk.) Pat which possesses anti‐tumor activities. There was no report its antitumor effect through regulating P‐glycoprotein (P‐gp) so far, due to P‐gp over expression is one of the most important mechanisms contributing to the multiple drug resistance phenotype. The present aim was to investigate the effects of TAB on P‐gp in multidrug‐resistant cells; Paclitaxel‐resistant cell line MDA‐MB‐231/Taxol was established by stepwise exposure for 10 months. MDA‐MB‐231 cells and MDA‐MB‐231/Taxol cells were treated with TAB, and their growth was evaluated using MTT assays. Paclitaxel accumulation in the cells was analyzed by high performance liquid chromatogram (HPLC). The activity of P‐gp was detected by intracellular accumulation of rhodamine123 (Rho123), and the protein expression of P‐gp was evaluated using western blot. Results indicated that the IC₅₀ of MDA‐MB‐231/Taxol to paclitaxel (Taxol) was 33 times higher than that of nature MDA‐MB‐231. TAB increased the intracellular concentration of Taxol and inhibited the activity of P‐gp and suppressed the expression of P‐gp in MDA‐MB‐231/Taxol cells. Our present results showed that TAB could reverse Taxol resistance in MDA‐MB‐231/Taxol cells, mainly inhibiting the activity of P‐gp and down‐regulating the expression level of P‐gp, and then enhancing the accumulation of chemotherapy agents. © 2013 The Authors Phytotherapy Research Published by John Wiley & Sons Ltd.     

少女原发性痛经主要中医证型及影响因素分析

目的探讨青春期少女痛经的主要中医证型及影响因素,以便为指导临床治疗及开展经期身心保健提供有效对策。方法采取问卷调查的方法,对笔者所在高校120名少女进行调查。结果剔除资料不足及盆腔有阳性发现者,共107名。中医证型以气滞血瘀和寒湿凝滞最多见,痛经程度越重,气滞、寒凝、血瘀则越明显;相关因素分析中有显著性意义的因素有经期未忌生冷、烦躁焦虑、月经失调,白带异常、睡眠不足、节食、偏食、母亲有痛经史、初潮年龄也有一定意义;痛经显著意义的影响因素与少女痛经主要中医证型一致。结论少女原发性痛经的主要中医证型为气滞血瘀和寒湿凝滞,经期未忌生冷、烦躁焦虑、月经失调、白带异常、睡眠不足、节食、偏食、母亲有痛经史、初潮年龄等对痛经有不同程度的影响,经期身心保健对痛经的治疗有重要意义。     

慢性肺源性心脏病PT_v_1改变的临床意义

目的探讨慢性肺源性心脏病PTFv1改变的临床意义。方法比较慢性肺源性心脏病PTFv1≤-0.04mm·s36例(观察组)和PTFv1正常36例(对照组)的LAD、LVEDD、E/A、LVEF值及治疗前后心律失常发生率.结果与对照组比较,观察组LAD、LVEDD显著增大(P<0.01),E/A、LVEF显著降低(P<0.01)。治疗前两组心律失常发生率差异无显著性意义(P>0.05),治疗后观察组心律失常消失者较对照组少,两组差异有显著性意义(P<0.01)。结论PTFv1≤-0.04mm·s提示慢性肺源性心脏病合并左心功能受损,PTFv1对慢性肺源性心脏病的治疗和随访具有重要指导意义。     

Compare two surgical interventions for otitis media with effusion in young children

European Archives of Oto-Rhino-Laryngology - To prospectively evaluate the effect of tympanostomy tubes combined with adenoidectomy and tube insertion on treatment for otitis media with effusion in...