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Hadi Shadan Alexander Andrew J. Vélez-Ortega A. Catalina Frolenkov Gregory I. 《Journal of the Association for Research in Otolaryngology》2020,21(2):121-135
Journal of the Association for Research in Otolaryngology - Mammalian hair cells develop their mechanosensory bundles through consecutive phases of stereocilia elongation, thickening, and... 相似文献
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A A Kosmachev G I Frolenkov E V Lukoshkova A A Liakishev V M Khaiutin 《Kardiologiia》1989,29(6):57-59
It was demonstrated that patients with coronary heart disease with coronary angiographically documented stenosing coronary atherosclerosis and frequent episodes of angina pectoris had significantly higher pain threshold in response to electric skin irritation and greater defense unconditioned flexor reflex R-III threshold than healthy subjects and patients with chest pain of extracoronary genesis. 相似文献
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Stepanyan RS Indzhykulian AA Vélez-Ortega AC Boger ET Steyger PS Friedman TB Frolenkov GI 《Journal of the Association for Research in Otolaryngology》2011,12(6):729-740
Aminoglycoside ototoxicity involves the accumulation of antibiotic molecules in the inner ear hair cells and the subsequent
degeneration of these cells. The exact route of entry of aminoglycosides into the hair cells in vivo is still unknown. Similar to other small organic cations, aminoglycosides could be brought into the cell by endocytosis or
permeate through large non-selective cation channels, such as mechanotransduction channels or ATP-gated P2X channels. Here,
we show that the aminoglycoside antibiotic gentamicin can enter mouse outer hair cells (OHCs) via TRPA1, non-selective cation
channels activated by certain pungent compounds and by endogenous products of lipid peroxidation. Using conventional and perforated
whole-cell patch clamp recordings, we found that application of TRPA1 agonists initiates inward current responses in wild-type
OHCs, but not in OHCs of homozygous Trpa1 knockout mice. Similar responses consistent with the activation of non-selective cation channels were observed in heterologous
cells transfected with mouse Trpa1. Upon brief activation with TRPA1 agonists, Trpa1-transfected cells become loaded with fluorescent gentamicin–Texas Red conjugate (GTTR). This uptake was not observed in mock-transfected
or non-transfected cells. In mouse organ of Corti explants, TRPA1 activation resulted in the rapid entry of GTTR and another
small cationic dye, FM1-43, in OHCs and some supporting cells, even when hair cell mechanotransduction was disrupted by pre-incubation
in calcium-free solution. This TRPA1-mediated entry of GTTR and FM1-43 into OHCs was observed in wild-type but not in Trpa1 knockout mice and was not blocked by PPADS, a non-selective blocker of P2X channels. Notably, TRPA1 channels in mouse OHCs
were activated by 4-hydroxynonenal, an endogenous molecule that is known to be generated during episodes of oxidative stress
and accumulate in the cochlea after noise exposure. We concluded that TRPA1 channels may provide a novel pathway for the entry
of aminoglycosides into OHCs. 相似文献
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Inna A. Belyantseva Benjamin J. Perrin Kevin J. Sonnemann Mei Zhu Ruben Stepanyan JoAnn McGee Gregory I. Frolenkov Edward J. Walsh Karen H. Friderici Thomas B. Friedman James M. Ervasti 《Proceedings of the National Academy of Sciences of the United States of America》2009,106(24):9703-9708
βcyto-Actin and γcyto-actin are ubiquitous proteins thought to be essential building blocks of the cytoskeleton in all non-muscle cells. Despite this widely held supposition, we show that γcyto-actin null mice (Actg1−/−) are viable. However, they suffer increased mortality and show progressive hearing loss during adulthood despite compensatory up-regulation of βcyto-actin. The surprising viability and normal hearing of young Actg1−/− mice means that βcyto-actin can likely build all essential non-muscle actin-based cytoskeletal structures including mechanosensory stereocilia of hair cells that are necessary for hearing. Although γcyto-actin–deficient stereocilia form normally, we found that they cannot maintain the integrity of the stereocilia actin core. In the wild-type, γcyto-actin localizes along the length of stereocilia but re-distributes to sites of F-actin core disruptions resulting from animal exposure to damaging noise. In Actg1−/− stereocilia similar disruptions are observed even without noise exposure. We conclude that γcyto-actin is required for reinforcement and long-term stability of F-actin–based structures but is not an essential building block of the developing cytoskeleton. 相似文献
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