实验性自身免疫性内耳病耳蜗热休克蛋白70的表达

目的　探讨自身免疫性内耳病模型动物耳蜗热休克蛋白的表达。方法　利用同种异体内耳抗原免疫豚鼠 ,以建立自身免疫性内耳病 (autoimmuneinnereardisease ,AIED)动物模型 ,并应用免疫组织化学及原位杂位技术 ,研究热休克蛋白 (heatshockprotein ,hsp) 70在正常对照组及AIED模型动物实验组耳蜗中的表达情况。结果　对照组豚鼠螺旋神经节细胞中存在hsp70样蛋白基础表达 ;实验组 2 8耳中 10耳 (35 7% )听阈提高≥ 10dB ;此组动物螺旋神经节细胞和血管纹、螺旋韧带hsp70及其mRNA表达明显增强。结论　以粗制膜迷路抗原免疫豚鼠 ,听阈提高动物hsp70在螺旋神经节细胞和血管纹、螺旋韧带合成增加。     

Expression of Intercellular Adhesion Molecule-1 in Immune Response of Inner Ear

To understand the role of intercellular adhesion molecule-1 (ICAM-1) in immune response of the inner ear, inner ear immune response was induced in rats by inoculation of keyhole limpet hemocyanine (KLH) into the scala tympani of the animals who had been systemically sensitized. The expression of ICAM-1 in the inner ear was immunohistochemically examined. ICAM-1 was found in the epithelium of the spiral modiolar vein (SMV) with its collecting venules (CVs) as early as 6 h after challenge. Expression of ICAM-1 was observed on the epithelium of the endolymphatic sac (ES) and perisaccular region at 12 h. The intensity of ICAM-1 staining reached its peak within 24-48 h in these sites of the inner ear. By day 28, most specimens were devoid of appreciable staining for ICAM-1.Our study demonstrates that adhesion molecules play an important role in extravasation of inflammatory cells from the systemic circulation in the process of inner ear immune response. It also shows that cytokines that control expression of     

纳络酮治疗弥漫性轴索损伤

目的 :观察纳络酮治疗弥漫性轴索损伤的疗效。方法 :15例为纳络酮组 ,其中男性 10例 ,女性 5例 ,年龄 (41.0±s 2 .0 )a ,格拉斯哥昏迷分级(GCS)为 (4.2± 1.5 )分。 18例为对照组 ,其中男性12例 ,女性 6例 ,年龄 (39± 4 )a ,GCS为 (4.1± 1.9)分。对照组给予 2 0 %甘露醇注射液 ,iv ,纳络酮组除给予 2 0 %甘露醇注射液静脉注射外 ,给予纳络酮注射液 4mg·d- 1,iv ,治疗时间为 2 0d。采用颅脑损伤转归分级 (GOS)的标准评价治疗结果。结果 :采用纳络酮治疗的病人 ,良好率为 6 0 % (9/ 15 ) ,对照组良好率为 17% (3/ 18)。 2组比较P <0 .0 5。未见不良反应。结论 :采用纳络酮治疗弥漫性轴索损伤的病人 ,能够明显地提高生活质量而没有任何不良反应     

实验性自身免疫性内耳病耳蜗热休克蛋白70的表达

目的 探讨自身免疫性内耳病模型动物耳蜗热休克蛋白的表达。方法 利用同种异体内耳抗原免疫豚鼠,以建立自身免疫性内耳病（ａｕｔｏｉｍｍｕｎｅ ｉｎｎｅｒ ｅａｒ ｄｉｓｅａｓｅ,ＡＩＥＤ）动物模型,并应用免疫组织化学及原位杂位技术,研究热休克蛋白（ｈｅａｔ ｓｈｏｃｋ ｐｍｔｅｉｎ,ｈｓｐ）７０在正常对照组及ＡＩＥＤ模型动物实验组耳蜗中的表达情况。结果 对照组豚鼠螺旋神经节细胞中存在ｈｓｐ７０样蛋白基