GC法测定大蒜β-环糊精包合物中大蒜素的含量

目的采用GC法测定大蒜β-环糊精包合物中的大蒜素(二烯丙基三硫化物)的含量。方法以0．5％己二酸二乙二醇聚酯为固定相，自制2米玻璃填充柱，以正己烷-无水乙醇(1：4)为溶剂，采用“超声提取法”处理样品。结果大蒜素线性范围为0．3～3．0mg／ml，r=0．9993，平均回收率为94．8％，RSD=4．61％(n=9)，最低检测限为0．2ng。结论本法简便、快速、灵敏，重现性好。     

大蒜素对巨噬细胞介导的细胞毒作用的影响

大蒜素单独或与其他激活剂协同作用时,具有增强巨噬细胞的抗肿瘤作用。同时可增强某些瘤细胞对巨噬细胞介导的细胞毒作用的敏感性。     

大蒜素衍生物烯丙基半胱氨酸壳寡糖亚微球的制备及其理化性质、体外释药特点和质量稳定性的考察

采用离子胶凝法制备了烯丙基半胱氨酸壳寡糖亚微球,以包封率和粒径为指标考察亚微球的处方和制备工艺,并对所制备的亚微球的理化性质、体外释药特点及质量稳定性进行研究.     

酸性微环境下大蒜素增强大鼠自然杀伤细胞的功能活性

目的 观察体外酸性培养环境下大蒜素对自然杀伤(NK)细胞功能活性的影响,并探讨其机制.方法 以pH 5.6、pH 6.5和pH 7.2的完全RPMI 1640培养基对大鼠脾脏CD3-NKR-P1+NK细胞进行悬浮培养,并给予30 mg/L浓度的大蒜素进行处理.酶联免疫吸附试验(ELISA)检测细胞培养液中干扰素(IFN)-γ的分泌水平,流式细胞仪检测NK细胞的增殖和凋亡率,乳酸脱氢酶法检测NK细胞的功能活性.结果 酸性培养环境下大鼠脾脏NK细胞的增殖能力明显下降,NK细胞功能活性明显受阻.在pH 7.2、pH 6.5和pH 5.6三种不同的培养条件下,大蒜素对NK细胞增殖率的影响表现为随培养环境的pH降低反而升高,以pH 5.6、培养16 h时达最高33.3%.与之相对应,NK细胞分泌的IFN-γ达(64.59±0.09)ng/L,较培养4 h时升高28%,且对小鼠淋巴瘤Yac-1细胞的杀伤活性也达到最高水平.结论 大蒜素可通过提高NK细胞IFN-γ分泌水平明显改善酸性培养环境下NK细胞的功能活性.

Abstract：

Objective To investigate the role of allicin on rat nature killer (NK) cell activity in vitro under acidic microenvironment, and its possible mechanism. Methods CD3- NKR-P1+ NK cells isolated from the rat spleen were cultured in the complete RPMI 1640 medium ( pH 5. 6, pH 6. 5, or pH 7. 2 respectively), and treated with allicin at final concentration of 30 mg/L. Enzyme linked immunosorbent assay (ELISA) was used to determine supernatant interferon (IFN)-γ levels. The percentage of NK cells proliferation and apopotosis was analyzed by flow cytometry. NK cell cytotoxicity toward YAC-1 tumor cells was detected by LDH release assay. Results Proliferation and cytotoxicity of NK cells were significantly suppressed by acidic microenvironment in vitro. Under the cultured condition of acidic pH below 7. 2, allicin seemed to promote NK cells proliferation, which reached to highest level of 33% at pH 5. 6 cultured for 16 h. Correspondingly, at pH of 5. 6, allicin induced a marked increase of IFN-γ concentration in the supernatant from (50. 07 ± 0. 38) (cultured for 4 h) to (64. 59 ± 0. 09) ng/L ( cultured for 16 h). The cytotoxicity of NK cells toward YAC-1 tumor cells was also found strongest under the condition of pH 5. 6 cultured for 16 h. Conclusion Allicin favored to enhance the cytotoxicity of NK cells under the acidic cultured condition, which might be related to the increase of IFN-γ production.     

大蒜素治疗婴幼儿秋季腹泻82例

目的探讨大蒜素治疗婴幼儿秋季腹泻的疗效。方法158例秋季腹泻患儿随机分成两组,以大蒜素治疗（治疗组）82例,并与病毒唑治疗者（对照组）76例作对比观察两组疗效。结果经1疗程治疗后,治疗组显效率、有效率和总有效率分别为74％,17％,91％;对照组显效率、有效率和总有效率45％,33％,78％,显效率、总有效率两组比较差异均有统计学意义（P〈0.05）。结论以大蒜素治疗婴幼儿秋季腹泻疗效满意。     

酸性微环境下大蒜素增强大鼠自然杀伤细胞的功能活性

Objective To investigate the role of allicin on rat nature killer (NK) cell activity in vitro under acidic microenvironment, and its possible mechanism. Methods CD3- NKR-P1+ NK cells isolated from the rat spleen were cultured in the complete RPMI 1640 medium ( pH 5. 6, pH 6. 5, or pH 7. 2 respectively), and treated with allicin at final concentration of 30 mg/L. Enzyme linked immunosorbent assay (ELISA) was used to determine supernatant interferon (IFN)-γ levels. The percentage of NK cells proliferation and apopotosis was analyzed by flow cytometry. NK cell cytotoxicity toward YAC-1 tumor cells was detected by LDH release assay. Results Proliferation and cytotoxicity of NK cells were significantly suppressed by acidic microenvironment in vitro. Under the cultured condition of acidic pH below 7. 2, allicin seemed to promote NK cells proliferation, which reached to highest level of 33% at pH 5. 6 cultured for 16 h. Correspondingly, at pH of 5. 6, allicin induced a marked increase of IFN-γ concentration in the supernatant from (50. 07 ± 0. 38) (cultured for 4 h) to (64. 59 ± 0. 09) ng/L ( cultured for 16 h). The cytotoxicity of NK cells toward YAC-1 tumor cells was also found strongest under the condition of pH 5. 6 cultured for 16 h. Conclusion Allicin favored to enhance the cytotoxicity of NK cells under the acidic cultured condition, which might be related to the increase of IFN-γ production.     

国内大蒜素对心脑血管疾病的临床治疗作用概况

综合分析国内大蒜素对心脑血管疾病的临床治疗作用概况。显示大蒜素对心血管有多方面药理作用,有抗凝血、抗高脂血症及抗冠心病心绞痛、抗急性病毒性心肌炎、降血压、抗心律失常和对急性脑梗死有临床治疗作用。认为大蒜作为中国传统的食物兼天然药物,其主要成分大蒜素对心血管病有很好的治疗作用,药物的开发利用将具有广阔前景。     

大蒜素辅助抗结核药物治疗肺结核疗效观察

目的观察大蒜素联合抗结核药物治疗肺结核的疗效及其副作用。方法对32例肺结核患者采用大蒜素肠溶胶丸和抗结核药物治疗,16例患者单用抗结核药物作为对照组治疗,治疗2个月后观察疗效,记录副作用。碱性磷酸酶-抗碱性磷酸酶(APAAP)方法检测淋巴细胞免疫状况。结果治疗组痰转阴率,症状改善率明显高于对照组。肝功能损伤发生率与对照组无明显差异。治疗2个月后,细胞免疫指标CD3、CD4、CD4/CD8较治疗前提。结论大蒜素肠溶胶丸联合抗结核药物治疗肺结核可提高成功率,增强细胞免疫功能。     

大蒜素辅助抗结核药物治疗肺结核疗效观察

目的观察大蒜素联合抗结核药物治疗肺结核的疗效及其副作用。方法对32例肺结核患者采用大蒜素肠溶胶丸和抗结核药物治疗,16例患者单用抗结核药物作为对照组治疗,治疗2个月后观察疗效,记录副作用。碱性磷酸酶-抗碱性磷酸酶(APAAP)方法检测淋巴细胞免疫状况。结果治疗组痰转阴率,症状改善率明显高于对照组。肝功能损伤发生率与对照组无明显差异。治疗2个月后,细胞免疫指标CD3、CD4、CD4/CD8较治疗前提。结论大蒜素肠溶胶丸联合抗结核药物治疗肺结核可提高成功率,增强细胞免疫功能。