首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   779篇
  免费   68篇
儿科学   3篇
基础医学   100篇
临床医学   36篇
内科学   55篇
皮肤病学   2篇
神经病学   16篇
特种医学   1篇
外科学   7篇
综合类   132篇
预防医学   445篇
药学   46篇
中国医学   4篇
  2024年   2篇
  2023年   3篇
  2022年   11篇
  2021年   12篇
  2020年   13篇
  2019年   31篇
  2018年   15篇
  2017年   17篇
  2016年   15篇
  2015年   21篇
  2014年   56篇
  2013年   56篇
  2012年   76篇
  2011年   58篇
  2010年   61篇
  2009年   67篇
  2008年   56篇
  2007年   58篇
  2006年   38篇
  2005年   26篇
  2004年   10篇
  2003年   11篇
  2002年   5篇
  2001年   11篇
  2000年   12篇
  1999年   9篇
  1998年   6篇
  1997年   9篇
  1996年   11篇
  1995年   4篇
  1994年   9篇
  1993年   27篇
  1992年   3篇
  1991年   3篇
  1990年   1篇
  1989年   2篇
  1988年   4篇
  1986年   2篇
  1985年   2篇
  1984年   2篇
  1983年   1篇
  1982年   1篇
  1981年   2篇
  1980年   1篇
  1978年   1篇
  1977年   1篇
  1976年   2篇
  1975年   2篇
  1973年   1篇
排序方式: 共有847条查询结果,搜索用时 15 毫秒
1.
目的分析常熟地区2005—2008年狂犬疫苗免疫效果和不同性别、年龄、年份以及季节等因素对免疫效果的影响。方法采用间接ELISA方法对5 471例全程足量注射狂犬疫苗者进行抗体检测分析。结果本调查人群总的抗体阳性率为94.3%,抗体阳性率女性高于男性,0~9岁高于其它年龄组。随着年份的增加,抗体阳性率呈增加趋势,同时,在季节分布上,第3季度抗体的阳性率低于其它3个季度。结论受动物致伤后要及时全程注射狂犬疫苗,免疫后检测血清抗体水平十分重要,对暂未产生抗体者要及时加强免疫,确保免疫效果。  相似文献   
2.
Summary The spread of rabies virus in the central nervous system of mice was examined after hindlimb footpad and intracerebral inoculation of the CVS strain of fixed rabies virus. All mice developed paralytic rabies. After intracerebral inoculation there was early simultaneous infection of neurons in the cerebral cortex and pyramidal neurons of the hippocampus, and later there was spread to the cerebellum. After high-dose intracerebral inoculation there was early infection ependymal cells lining the lateral ventricles and neurons adjacent to the central canal of the spinal cord, suggesting that rabies virus entry into the CNS occurs, at least in part, by a cerebrospinal fluid pathway. The sequence of involvement was different after hindlimb footpad inoculation. Infection became established in the cerebellum on day 5, in the cerebral cortex on day 6, and in the hippocampus on day 8. CA3 was initially affected, CA1 became infected 2 days later, and there was much less involvement of the dentate gyrus. Hippocampal infection occurred late relative to the rest of the brain after peripheral inoculation, but not after intracerebral inoculation. The hippocampus is not a good location for the detection of early brain infection after peripheral inoculation, although it may be involved when a natural rabies vector has the ability to transmit infection. These findings also raise questions about the mechanisms for the limbic dysfunction observed in clinical rabies.Supported by grant MA-10068 from the Medical Research Council of Canada  相似文献   
3.
In Europe, three genotypes of the genus Lyssavirus, family Rhabdoviridae, are present, classical rabies virus (RABV, genotype 1), European bat lyssavirus type 1 (EBLV-1, genotype 5) and European bat lyssavirus type 2 (EBLV-2, genotype 6). The entire authentic nucleoprotein (N protein) encoding sequences of RABV (challenge virus standard, CVS, strain), EBLV-1 and EBLV-2 were expressed in yeast Saccharomyces cerevisiae at high level. Purification of recombinant N proteins by caesium chloride gradient centrifugation resulted in yields between 14-17, 25-29 and 18-20 mg/l of induced yeast culture for RABV-CVS, EBLV-1 and EBLV-2, respectively. The purified N proteins were evaluated by negative staining electron microscopy, which revealed the formation of nucleocapsid-like structures. The antigenic conformation of the N proteins was investigated for their reactivity with monoclonal antibodies (mAbs) directed against different lyssaviruses. The reactivity pattern of each mAb was virtually identical between immunofluorescence assay with virus-infected cells, and ELISA and dot blot assay using the corresponding recombinant N proteins. These observations lead us to conclude that yeast-expressed lyssavirus N proteins share antigenic properties with naturally expressed virus protein. These recombinant proteins have the potential for use as components of serological assays for lyssaviruses.  相似文献   
4.
用免疫酶组化染色ABC法,研究了3例狂犬病人舌及3例涎腺中狂犬病毒抗原(RVAg)的分布。在2例4/5舌切片中见其浆液腺细胞、末梢神经、少数横纹肌纤维及复盖上皮包括味细胞中含大量RVAg颗粒,1例舌切片中无浆液腺,为阴性反应。在3例4/9大涎腺切片中只在腺叶内或末梢神经有少量弱反应的RVAg。与以往报告的病犬、狐、臭鼠大涎腺中含大量RVAg不同。人大涎腺中RVAg含量远比舌中为少。人舌浆液腺区可能是RVAg多见部位,可能是一传染源。  相似文献   
5.
目的分析桂林市2002~2005年狂大病免疫失败的危险因素,为制定预防控制措施提供科学依据。方法对桂林市各县(区)2002~2005年35例接种狂犬疫苗失败者及42例对照者进行狂犬病免疫失败相关危险因素个案调查,包括病例的一般情况、致伤部位、致伤程度和伤口处理情况、免疫接种等,运用非条件logistic回归分析法对数据进行统计分析。结果10项研究指标中有5项单因素分析有统计学意义,3项多因素分析有统计学意义,分别是:伤口处理、注射第一剂疫苗时间和是否注射血清或免疫球蛋白。结论伤口不处理或未正确处理、未及时接种狂犬疫苗和未注射抗狂犬病血清或免疫球蛋白是发生免疫失败的主要原因。  相似文献   
6.
目的了解2003年广州市疾病预防控制中心门诊部接诊的8417例狂犬病疫苗接种者中2397例学生的流行病学特征,为我市狂犬病预防提供信息和依据。方法对广州市疾病预防控制中心门诊部2003年狂犬病疫苗接种者中2397例登记资料进行分析。结果动物致伤者中学生28·48%,占总人数的1/3。结论在学校宣传狂犬病疫苗接种常识是十分重要的预防措施。  相似文献   
7.
Pathological analysis of experimental infections has been a useful method in virology. Host selection and route of virus inoculation are the most important determinants in experimental paradigms. Clinical and pathological correlations must be carefully determined in human and experimental infections. The cell tropism, cytopathic effect, and pathogenicity of viruses could be evaluated and defined by analysis of infected tissue. In addition, these infected tissues could be used as positive control for immunohistochemistry and in situ hybridization analysis in diagnostic pathology. This review addresses animal paradigms of rabies, influenza, and poliomyelitis as representatives of zoonosis and human-specific infection with respect to animal selection for experimental infections.  相似文献   
8.
Dog vaccination and population management have been suggested as priorities in attempts at disease control in canine rabies-endemic countries. Budget limitations and the complexity of social, cultural and religious variables have complicated progress in the developing world. In Bangkok, Thailand, an intensive canine vaccination and sterilization programme has been in place since November 2002. Our objective was to determine if the rabies virus could be mapped according to its genetic variations and geographical location on the small localized scale of Bangkok and its surrounding provinces. Phylogenetic characterization of 69 samples from Bangkok and five neighbouring and two remote provinces, by limited sequence analysis of the rabies virus nucleoprotein gene, distinguished six different clades. Rabies viruses of four clades were intermixed in Bangkok and in the surrounding highly populated regions whereas the other two clades were confined to rural and less populated provinces. Such a complex pattern of gene flow, particularly in Bangkok, may affect the outcome of canine control programmes.  相似文献   
9.
茶陵县2000至2004年狂犬病及其相关危险因素病例对照研究   总被引:1,自引:0,他引:1  
目的研究湖南省茶陵县狂犬病发生的相关危险因素,为现场干预研究提供科学依据。方法以2000~2004年茶陵县全部狂犬病病例为研究对象,以与病例组具有同源犬伤暴露史的健康存活者为对照组,进行病例对照研究,统计学分析采用χ2检验。结果病例组平均潜伏期为60d(9~412d),平均病程为2d(0~7d),其中头面部、手部和上肢、躯干和下肢受伤者平均潜伏期分别为28、52和77d,浅表伤口与深度伤口患者平均潜伏期分别为56和68d,有狂犬病疫苗接种史与无狂犬病疫苗接种史患者的平均潜伏期分别为39和62d。相关危险因素分析,未接种狂犬病疫苗(χ2=48.776,P<0.01,OR=41.67)、伤口部位接近中枢神经(χ2=35.143,P<0.01)、深度伤(χ2=6.800,P<0.05,OR=3.23)和未进行伤口处理(χ2=6.174,P<0.05,OR=2.84)等因素相对危险性明显高于对照组,差异均有显著统计学意义。结论未接种狂犬病疫苗、接种非正规途径来源的狂犬病疫苗、伤口未处理、深度伤口、伤口位置靠近中枢神经等为茶陵县狂犬病发生的相关危险因素。应重点加强犬伤暴露后的伤口规范化处理及接种狂犬病疫苗等防制措施。  相似文献   
10.
Targeting DNA vaccines to dendritic cells (DCs) greatly enhances immunity. Although several approaches have been used to target protein Ags to DCs, currently there is no method that targets DNA vaccines directly to DCs. Here, we show that a small peptide derived from the rabies virus glycoprotein fused to protamine residues (RVG‐P) can target DNA to myeloid cells, including DCs, which results in enhanced humoral and T‐cell responses. DCs targeted with a DNA vaccine encoding the immunodominant vaccinia B8R gene via RVG‐P were able to restimulate vaccinia‐specific memory T cells in vitro. Importantly, a single i.v. injection of B8R gene bound to RVG‐P was able to prime a vaccinia‐specific T‐cell response that was able to rapidly clear a subsequent vaccinia challenge in mice. Moreover, delivery of DNA in DCs was enough to induce DC maturation and efficient Ag presentation without the need for adjuvants. Finally, immunization of mice with a DNA‐vaccine encoding West Nile virus (WNV) prM and E proteins via RVG‐P elicited high titers of WNV‐neutralizing Abs that protected mice from lethal WNV challenge. Thus, RVG‐P provides a reagent to target DNA vaccines to myeloid cells and elicit robust T‐cell and humoral immune responses.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号