目的通过临床研究评价Rely X ARC粘接系统对Ⅱ类洞嵌体的粘接效果。方法30个后牙Ⅱ类洞使用同种金合金嵌体及Rely X ARC粘接系统修复后,通过改良的USPHS标准评估嵌体粘接后2周、6个月、1年的临床指标。结果所有嵌体6个月和1年的各项指标得分均在临床可接受的范围内。结论Rely X粘接系统对于金合金嵌体修复后牙Ⅱ类洞有比较可靠的短期效果,长期效果有待继续验证。 相似文献
Hyperplasia suppressor gene (HSG), also called human mitofusin 2, is a novel gene that markedly suppresses the cell proliferation of hyperproliferative vascular smooth muscle cells from spontaneously hypertensive rat arteries. This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. In this report, we showed that an adenovirus vector encoding human HSG (Ad5-hHSG) had an antitumor activity in a wide range of cancer cell lines. We further focused on the lung cancer cell line A549 and the colon cancer cell line HT-29 and then observed that Ad5-hHSG induced apoptosis both in vitro and in vivo. Confocal laser scanning microscopy and electron microscopy revealed that cells infected with Ad5-hHSG formed dose-dependent perinuclear clusters of fused mitochondria. Adenovirus-mediated hHSG overexpression induced apoptosis, cell cycle arrest, mitochondrial membrane potential (DeltaPsim) reduction and release of cytochrome c, caspase-3 activation, and cleavage of PARP in vitro. Overexpression of hHSG also significantly suppressed the growth of subcutaneous tumors in nude mice both ex vivo and in vivo. In addition, Ad5-hHSG increased the sensitivity of these cell lines to two chemotherapeutic agents, VP16 and CHX, and radiation. These results suggest that Ad5-hHSG may serve as an effective therapeutic drug against tumors. 相似文献
Osteogenesis imperfecta (OI) is a congenital disorder caused most often by dominant mutations in the COL1A1 or COL1A2 genes that encode the alpha chains of type I collagen. Severe forms of OI are associated with skeletal deformities and frequent fractures. Skeletal pain can occur acutely after fracture, but also arises chronically without preceding fractures. In this study we assessed OI-associated pain in the Col1a1Jrt/+ mouse, a recently developed model of severe dominant OI. Similar to severe OI in humans, this mouse has significant skeletal abnormalities and develops spontaneous fractures, joint dislocations and vertebral deformities. In this model, we investigated behavioral measures of pain and functional impairment. Significant hypersensitivity to mechanical, heat and cold stimuli, assessed by von Frey filaments, radiant heat paw withdrawal and the acetone tests, respectively, were observed in OI compared to control wildtype littermates. OI mice also displayed reduced motor activity in the running wheel and open field assays. Immunocytochemical analysis revealed no changes between OI and WT mice in innervation of the glabrous skin of the hindpaw or in expression of the pain-related neuropeptide calcitonin gene-related protein in sensory neurons. In contrast, increased sensitivity to mechanical and cold stimulation strongly correlated with the extent of skeletal deformities in OI mice. Thus, we demonstrated that the Col1a1Jrt/+ mouse model of severe OI has hypersensitivity to mechanical and thermal stimuli, consistent with a state of chronic pain. 相似文献
Acute myeloid leukemia (AML) is a heterogeneous blood disease with poor treatment effect and high recurrence rate. With the deepening of non-coding RNA research, more and more miRNAs have been found to participate in various physiological processes of tumors. In this study, we tried to find the miRNA related to the prognosis of AML.
Methods
Collect gene expression data and clinical information of AML patients in the Cancer Genome Atlas database for statistical analysis. The expression level of miR-195 of each patient was standardized by logCPM and then produced as a box plot according to subtype classification. TargetScan was used to predict the target genes of miR-195, and these genes were subjected to GO pathway enrichment analysis by Metascape. Differential miRNAs were screened through the DESeq2 package in the R language. Survival rates were estimated using the Kaplan–Meier method and the log-rank test. The multivariate Cox proportional hazard models of EFS and OS were established.
Results
We found that the expression of miR-195 was the lowest in cytogenetically normal (CN-) AML, and high expression of miR-195 only promoted the prognosis of chemotherapy-only CN-AML patients (EFS: P?=?0.016; OS: P?=?0.035). Multivariate analysis showed that miR-195high was a favorable and independent factor for CN-AML (both P?<?0.05). Further analysis showed that miR-195 may affect signal transduction through ANHAK2 in AML.
Conclusion
We found that high expression of miR-195 can increase prognosis time of chemotherapy-only CN-AML patients, providing a new possibility for treatment.
Two compatible conversion factors for converting diameter measurements taken at different breast heights were derived for Pinus radiata using taper data from more than 3000 trees. The two breast heights used for conversion were 1.3 and 1.4 m above ground, as defined in Australia and New Zealand, two major radiata-growing countries in the world. The conversion factors were estimated through three alternative statistical methods including simple least squares regression, seemingly unrelated regression and errors-in-variables models. The three sets of estimates were almost identical and had similar conversion accuracy, although the second method was slightly better. The conversion factors were more accurate than overbark taper equations used for the same purpose. The factor was 0.9916 for converting diameter measured at 1.3 to that at 1.4 m above ground, and the inverse of this value, 1.0084, was for the vice versa. When calculating tree and stand volume and biomass using equations with diameter at a different breast height as a predictor to that of the input data, the bias, either over or under estimation, could be between 1.67% and 2.00% without conversion. These conversion factors will facilitate the sharing of data among radiata growing countries with different definitions of breast height, but more importantly it will help correct the bias in stand volume and biomass estimation caused by the seemingly negligible difference in breast height when software for forest resource management and decision support developed in one country is applied in another. Such bias when accumulated over a large management area may not be financially insignificant for an astute forest management agency. 相似文献