穿透性角膜移植术后角膜内皮细胞之命运

地２组猴行５。５→５．０ｍｍ穿透性角膜移植术，异种组４只，同种组４只，术前用冷冻创伤＾３Ｈ－ＴｄＲ标记法标记受生角膜内皮细胞，术后１４周摘除眼球，用放射自显影法显示银颗粒的分布。     

组织多肽特异性抗原在乳腺癌中的临床研究

OBJECTIVE: To investigate the expression of serum tissue polypeptide-specific antigen (TPS) in breast cancer patients and its clinical value in such cases. METHODS: Altogether 160 subjects (90 patients with breast cancer, 40 with benign breast lesions, and 30 healthy subjects) were enrolled in this study. The serum TPS and CA153 levels were measured by ELISA in all the subjects. RESULTS: The levels and positivity rate of serum TPS and CA153 in breast cancer group were significantly higher than those in the normal subjects group and benign lesion group (P<0.01), and became even higher as the malignancy progressed. High serum TPS level was detected in the cancer patients in stage I. Serum TPS level was the most sensitive to bone metastasis of the malignancy, but its highest levels occurred in cases of lymphoid node metastasis (P<0.05). In patients who responded favorably to the treatment, serum TPS and CA153 levels were significantly reduced (P<0.05), but the reduction in TPS levels tended to be more obvious (P<0.05). CONCLUSION: Serum TPS can be used as a very useful and sensitive tumor marker in the diagnosis of breast cancer, especially in case of bone metastasis, and may be of great value in clinical decision-making and assessment of therapeutic effect.     

先天性齿槽突裂整复术(附10例报告)

应用自体髂松质骨髓颗粒植入整复10例单侧上颌齿槽突裂,并关闭口鼻瘘。10例伤口一期愈合,术后观察3~6个月,外观改善明显,X线检查有明显骨形成,骨密度近似正常骨组织,两者间无明显界限。     

腰骶部SPR术中脊神经前后根定位的应用解剖

目的：为ＳＰＲ提供可靠的术中脊神经前、后根鉴别的解剖学依据。方法：在２０例成人脊柱标本上,去除后部结构,暴露整个马尾神经,对Ｌ１～Ｓ２节段的前后根进行形态学观察和测量。结果：脊神经后根位于马尾的后半部,前根则位于前半部。脊神经后根较相应的前根粗大,后根从Ｌ１～Ｓ１逐渐增大,以Ｓ１为最粗大;前根则以Ｌ３最粗大。相应前后根出硬脊膜前,有一段相互贴附并紧贴硬脊膜侧壁。结论：在多椎板切除ＳＰＲ术中前、后根的定位及鉴别,暴露时可根据前、后根出硬脊膜前的相互贴附;在限制性椎板切除时则可通过脊髓外侧索和Ｌ１前、后根之间的最下端的齿状韧带加以鉴别     

医学图书馆与PubMed本地化服务

医学图书馆通过在PubMed中提供馆藏链接、设立MyNCBI共享账号以及为用户提供PubMed使用培训和检索咨询服务,从而实现PubMed的本地化服务。     

Four-Dimensional Computerized Tomography （4D-CT） Reconstruction Based on the Similarity Measure of Spatial Adjacent Images

Objective：To investigate the feasibility of a 4D-CT reconstruction method based on the similarity principle of spatial adjacent images and mutual information measure. Methods：A motor driven sinusoidal motion platform made in house was used to create one-dimensional periodical motion that was along the longitudinal axis of the CT couch. The amplitude of sinusoidal motion was set to an amplitude of ±1 cm. The period of the motion was adjustable and set to 3.5 s. Phantom objects of two eggs were placed in a Styrofoam block, which in turn were placed on the motion platform. These objects were used to simulate volumes of interest Undergoing ideal periodic motion. CT data of static phantom were acquired using a multi-slice general electric （GE） LightSpeed 16-slice CT scanner in an axial mode. And the CT data of periodical motion phantom were acquired in an axial and cine-mode scan. A software program was developed by using VC ＋ ＋ and VTK software tools to resort the CT data and reconstruct the 4D-CT. Then all of the CT data with same phase were sorted by the program into the same series based on the similarity principle of spatial adjacent images and mutual information measure among them, and 3D reconstruction of different phase CT data were completed by using the software. Results：All of the CT data were sorted accurately into different series based on the similarity principle of spatial adjacent images and mutual information measures among them. Compared with the unsorted CT data, the motion artifacts in the 3 D reconstruction of sorted CT data were re- duced significantly, and all of the sorted CT series result in a 4D-CT that reflected the characteristic of the periodical motion phantom. Conclusion：Time-resolved 4D-CT reconstruction can be implemented with any general multi-slice CT scanners based on the similarity principle of spatial adjacent images and mutual information measure. The process of the 4D-CT data acquisition and reconstruction were not restricted to the hardware or software of the C     

周围神经干自然分束部位血管形态及临床意义

应用微血管灌注透明法观察研究5具成人周围神经干标本.重点观察神经自然分束部位微血管形态和分支分布规律.神经干内有非常完兽的血供系统.手术中循神经自然分束进行追踪分离,不会对神经血供产生明显影响.但在张力下牵拉缝合神经,将对神经干血供产生严重影响.神经束膜缝合比神经外膜缝合更强调在无张力下进行.     

Rapid, accurate genotyping of the common -alpha(4.2) thalassaemia deletion based on the use of denaturing HPLC

AIMS: To develop an alternative assay for specific genotyping of the -alpha(4.2) thalassaemia deletion based on the DNA sequence features surrounding the breakpoint. METHODS: The 5' and 3' ends of the breakpoint regions of the -alpha(4.2) allele and the normal homologous segments were sequenced in Chinese individuals. A sequence haplotype composed of four single nucleotide variations within the X2/X1 box of the -alpha(4.2) breakpoint region was found in all of the 10 Chinese -alpha(4.2) thalassaemia alleles studied. Based on these findings, a novel polymerase chain reaction (PCR)/denaturing high performance liquid chromatography (DHPLC) assay was developed for rapid genotyping of the -alpha(4.2) allele instead of traditional Southern blotting or Gap-PCR. This method involves amplification of the alpha globin target sequence encompassing these four polymorphic sites, followed by a partially denaturing HPLC analysis using the transgenomic WAVE DNA fragment analysis system. RESULTS: The three major genotypes (-alpha4.2/alphaalpha, -alpha(4.2)/--SEA, and alphaalpha/alphaalpha) could be distinguished through the characteristic chromatograms generated by the WAVE system. The accuracy of this technique was evaluated blindly, and the results were 100% (40 of 40) concordant with the genotypes previously characterised by Southern blotting or Gap-PCR. CONCLUSIONS: This study validates the PCR/DHPLC approach as a simple, rapid, highly accurate, and cost effective method, potentially adaptable for use in epidemiological surveys, genetic screening, and diagnosis of silent alpha+ thalassaemia and Hb H disease.     

Therapeutic effects of radio frequency ablation in lung tumor treatment

Radiofrequencyablation(RFA)isarecentlyde-velopedsystemthatprovidesanewapproachforthetreatmentofpatientswithunresectablemalignanttu-mor.Aninsulatedneedleelectrodethatcontains10fineneedleswithinentersthetumortissueunderCTguidanceandthe10fineneedlesstretchasanumbrel-lacoveringanareaapproximately3.5cmindiameter,beforearadiofrequencygeneratortransmitshigh-en-ergyradiofrequencypulsesthroughthewiretothefineneedles.Theenergyreleasedattheneedlepointcantriggerionicagitationandfrictionalheattopro-ducea…     

Differences between the gap junctional communication function of fibroblasts derived from pathological scars and normal skin

Keloid is a fibrotic disease Of the hUInan dends thattakes the form of benign fibIOus tumors resulting from anabnolmal response to wound. Unlike hyperplastic scarewith which keloid is freqUently collfused, keloid lesionusually extends beyond the bountyyes of the originalwound by invasive growth without rendssion with time,which is diffictilt to cormct s~cally and does not proVOke scar .on~tionll'2].It is noted that there ~ vallous patterns of intereellular interachon, as junchonal interoell…