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排序方式: 共有677条查询结果,搜索用时 15 毫秒
1.
Bezirtzoglou E Cretoiu SM Moldoveanu M Alexopoulos A Lazar V Nakou M 《Journal of dentistry》2008,36(8):600-605
OBJECTIVES: Toothbrushes are rapidly contaminated with different microorganisms, which colonize the oral cavity and interdental spaces. This can represent a possible cause of infection or reinfection. In this study, the ozone experimental effect upon toothbrushes microflora was estimated microbiologically before and after saturation with ozone gas. METHODS: Fifty used toothbrushes coming from children and adults were entered our study. Microorganisms were enumerated and identified. Bristles from each brush were soaked in ozone saturated PBS solution for 5, 10, 15, 20 and 30 min and the total microbial population was reassessed. RESULTS: Counts of microorganisms isolated per brush varied between 10(2) and 10(7) CFU. Candida albicans was present in used toothbrushes. No obligate anaerobes were isolated. Members of Streptococcaceae family were regularly found (65.2%) belonging to the following species: Streptococcus pyogenes, S. mutans, S. mitis, S. oralis, S. sobrinus, S. viridans, S. salivarius, S. sanguis, Aerococcus viridans. A. viridans and S. mutans were more frequently isolated on children toothbrushes while Staphylococcus aureus and S. epidermidis were found on adults brushes. Escherichia coli, Pseudomonas sp. and Enterococcus sp., were also recovered. We found that the ozone treatment decreased gradually the microbial load. However, a bacterial re-growth was effective following short ozonation period. Decontamination was complete after an extended exposure to ozone for 30 min. CONCLUSIONS: Ozone application was found to remove the toothbrushes bristles microbiota following conventional brushing. Maximum decontamination efficacy of ozone treatment was observed after 30 min while exposure for short time periods seems to be inefficient which probably reflect the low dose of ozone used in this study. 相似文献
2.
饮用水臭氧消毒效果的影响因素 总被引:1,自引:1,他引:0
目的观察臭氧在不同因素的作用下对水中细菌的杀灭情况,为指导臭氧发生器的正确使用提供基础数据。方法以大肠杆菌8099为指示菌,观察作用时间(2.5~30min)、水温(10~40℃)、pH值(6.5~8.5)、臭氧通气流量(1.0~3.0L/min)等因素对臭氧杀菌效果的影响。结果臭氧在水温20℃,pH=7.0,臭氧流量1L/min的条件下,5min对大肠杆菌的杀灭率为99.99%~100%;水温为10~40℃时,臭氧对大肠杆菌的杀灭率随温度升高而下降;pH值为6.5和7.0时,对大肠杆菌的杀灭率为100%,pH=8.5时,杀灭率为99.99%;臭氧流量为1.0~3.0L/min时,杀灭率随臭氧通气流量的升高而下降。结论低温、低pH值下,臭氧的杀菌效果相对较好;臭氧在大通气流量下杀菌效果较差。 相似文献
3.
Maya Margalit Eyal Attias Drorit Attias Deborah Elstein Ari Zimran Yaacov Matzner 《International journal of laboratory hematology》2001,23(4):243-247
The application of ozone is widely practised as a form of alternative medicine, particularly in Germany and Eastern Europe. Ozone major autohemotherapy (the return of a small amount of a patient’s blood to the circulation after ex vivo exposure to ozone) has been reported to have a therapeutic effect in various pathological conditions, including ischemic, infectious, autoimmune and neoplastic disorders. Ozone has an effect on the expression of cytokines, adhesion molecules and acute phase reactants, which are responsible in part for the respiratory inflammatory response observed after exposure to this gas. The purpose of the present study was to investigate the effect of ozone administration ex vivo, at a concentration commonly used in major autohemotherapy, on peripheral blood neutrophil function in vitro. Blood drawn from healthy volunteers was studied for neutrophil adhesion, chemotaxis and O?2 production before and after exposure to 30 μg/ml ozone. There was no significant difference in adhesion and chemotaxis of neutrophils exposed to ozone versus unexposed cells. O?2 production was minimally decreased (20.3 ± 5.0 vs. 22.1 ± 5.5 nmol/106 cells/10 min, respectively; P=0.01), a reduction of no clinical significance. This study confirms that major autohemotherapy with ozone is safe as far as neutrophil function is concerned. Combined with previous data, it seems that well‐designed clinical trials to assess the efficacy of major autohemotherapy would pose no danger to blood cell populations, and should be encouraged. 相似文献
4.
目的:研究臭氧联合阿德福韦酯胶囊对乙肝的治疗效果。方法:将同期就诊的112例患者按志愿接受治疗为原则,分为治疗组与对照组。治疗组:38例患者,予臭氧自血疗法联合阿德福韦酯胶囊,臭氧自血疗法疗程3个月,阿德福韦酯胶囊疗程18个月;对照A组:34例患者,单独使用口服阿德福韦酯胶囊,疗程18个月;对照B组:40例患者.单独使用臭氧自血疗法,疗程3个月。比较三组治疗后各项指标的变化。结果:治疗组联合应答率为47.3%;对照A组为38.2%:对照B组为40.0%。结论:臭氧自血疗法联合阿德福韦酯胶囊治疗乙肝疗效与单用阿德福韦酯胶囊近期疗效比较,差异具有统计学意义(P〈0.05)。该治疗方法起效快,值得临床应用。 相似文献
5.
臭氧对家蝇生物学效应的研究 总被引:1,自引:0,他引:1
目的:研究臭氧(O3)对家蝇的生物学效应。方法:用一定浓度的臭氧对家蝇的卵、蛹、成虫作用一定时间,分别观察其成活率及子代的翅脉变异。结果:O3对家蝇卵有致死作用;O3对蛹有一定杀伤作用,使羽化受到明显抑制,羽化时间相对延长;O3对成蝇有一定致死作用,存活的家蝇进行传代,子代F1、F2、F3均出现短翅和翅脉变异,但对于代性别无明显影响。结论:家蝇的卵、蛹、成虫分别用O3处理,产生不同程度生物学效应,可以认为O3对家蝇子代有诱变作用。 相似文献
6.
Larson SD Schelegle ES Walby WF Gershwin LJ Fanuccihi MV Evans MJ Joad JP Tarkington BK Hyde DM Plopper CG 《Toxicology and applied pharmacology》2004,194(3):211-220
Nerves and neuroendocrine cells located within the airway epithelium are ideally situated to sample a changing airway environment, to transmit that information to the central nervous system, and to promote trophic interactions between epithelial and mesenchymal cellular and acellular components. We tested the hypothesis that the environmental stresses of ozone (O(3)) and house dust mite allergen (HDMA) in atopic infant rhesus monkeys alter the distribution of airway nerves. Midlevel bronchi and bronchioles from 6-month-old infant monkeys that inhaled filtered air (FA), house dust mite allergen HDMA, O(3), or HDMA + O(3) for 11 episodes (5 days each, 0.5 ppm O(3), 8 h/day followed by 9 days recovery) were examined using immunohistochemistry for the presence of Protein gene product 9.5 (PGP 9.5), a nonspecific neural indicator, and calcitonin gene-related peptide (CGRP). Along the axial path between the sixth and the seventh intrapulmonary airway generations, there were small significant (P < 0.05) decrements in the density of epithelial nerves in monkeys exposed to HDMA or O(3), while in monkeys exposed to HDMA + O(3) there was a greater significant (P < 0.05) reduction in epithelial innervation. In animals exposed to O(3) or HDMA + O(3) there was a significant increase in the number of PGP 9.5 positive/CGRP negative cells that were anchored to the basal lamina and emitted projections in primarily the lateral plain and often intertwined with projections and cell bodies of other similar cells. We conclude that repeated cycles of acute injury and repair associated with the episodic pattern of ozone and allergen exposure alter the normal development of neural innervation of the epithelial compartment and the appearance of a new population of undefined PGP 9.5 positive cells within the epithelium. 相似文献
7.
Valacchi G Vasu VT Yokohama W Corbacho AM Phung A Lim Y Aung HH Cross CE Davis PA 《Toxicology and applied pharmacology》2007,222(2):227-234
Despite the physiological importance of alpha-tocopherol (AT), the molecular mechanisms involved in maintaining cellular and tissue tocopherol levels remain to be fully characterized. Scavenger receptor B1 (SRB1), one of a large family of scavenger receptors, has been shown to facilitate AT transfer from HDL to peripheral tissues via apo A-1-mediated processes and to be important in the delivery of AT to the lung cells. In the present studies the effects of age and two environmental oxidants ozone (O(3)) (0.25 ppm 6 h/day) and cigarette smoke (CS) (60 mg/m(3) 6 h/day) for 4 days on selected aspects of AT transport in murine lung tissues were assessed. While AT levels were 25% higher (p<0.05) and 15% lower (p<0.05) in plasma and lung tissue, respectively, in aged versus young mice, acute environmental exposure to O(3) or CS at the doses used had no effect. Gene expression levels, determined by RT-PCR of AT transport protein (ATTP), SRB1, CD36, ATP binding cassette 3 (ABCA3) and ABCA1 and protein levels, determined by Western blots for SRB1, ATTP and ABCA1 were assessed. Aged mouse lung showed a lower levels of ATTP, ABCA3 and SRB1 and a higher level CD36 and ABCA1. Acute exposure to either O(3) or CS induced declines in ATTP and SRB1 in both aged and young mice lung. CD36 increased in both young and aged mice lung upon exposure to O(3) and CS. These findings suggest that both age and environmental oxidant exposure affect pathways related to lung AT homeostasis and do so in a way that favors declines in lung AT. However, given the approach taken, the effects cannot be traced to changes in these pathways or AT content in any specific lung associated cell type and thus highlight the need for further follow-up studies looking at specific lung associated cell types. 相似文献
8.
目的探讨虎蛇解毒方配用体外循环三氧对慢性乙型肝炎患者肝功能的保护作用及对HBV复制的抑制作用。方法以清热凉血解毒,疏肝健脾补肾为法,自拟虎蛇解毒方,观察其配用体外循环三氧对慢性乙型肝炎患者HBV复制的抑制作用、肝功能的保护作用以及患者免疫功能的变化,并设干扰素组及常规护肝组进行疗效比较。结果虎蛇解毒方配用体外循环三氧组HBeAg、HBV DNA转阴率、ALT复常率及临床有效率分别为46.9%、67.5%、85%及85%;干扰素组HBeAg、HBV DNA转阴率、ALT复常率及临床有效率分别为32.3%、34.2%、71.7%及60.53%;常规护肝组HBeAg、HBV DNA转阴率、ALT复常率及临床有效率分别为5.9%、7.1%、50%及23.81%。经统计学处理,虎蛇解毒方配用三氧治疗组ALT复常率、HBV DNA、HBeAg的转阴率、免疫功能指标CD3、临床效果较常规护肝组明显提高(P0.01),与干扰素组比较,ALT复常率、HBeAg的转阴率差异无统计学意义(P0.05);但HBV DNA临床效果、免疫功能指标CD3差异有统计学意义(P0.05或P0.01)。结论虎蛇解毒方配用体外循环三氧疗法对HBV有一定的抑制作用,对肝功能及免疫功能有改善作用,并可作为治疗慢性乙型肝炎的一种重要的治疗方法 。 相似文献
9.
目的 评价侧隐窝注射臭氧(O3)治疗腰椎间盘突出症致神经根炎对糖尿病患者血糖的影响.方法 腰椎间盘突出症致神经根炎患者96例,糖尿病和非糖尿病患者各48例,糖尿病和非糖尿病患者分别采用随机数字表法各分为2组(n=24),非糖尿病-O3组(N-O3组)、非糖尿病-糖皮质激素组(N-GC组)和糖尿病-O3组(D-O3组)、糖尿病-糖皮质激素组(D-GC组).N-O3组和D-O3组分别于侧隐窝注射30 mg/L O3 10Ml,N-GC组和D-GC组分别于侧隐窝注射复方倍他米松注射液3.5 mg.于治疗前(T1)、治疗后1 h(T3)、4 h(T4)、治疗后1 d(T5)、3 d(T6)、7 d(T7)时测定血糖.于T1、治疗后即刻(T2)、T5、T6、T7时行VAS评分,于T1和T7时行健康调查简表评分,于T7时评定治疗效果,记录不良反应的发生情况.结果 与T1时比较,4组治疗后VAS评分降低,T7时生理机能、躯体疼痛、情感职能、精神健康评分及N-GC组的一般健康状况评分、D-O3组和N-O3组的精力评分升高,N-GC组、D-O3组T4时及D-GC组T3~7时血糖升高(P<0.05).N-O3组治疗前、后血糖比较差异无统计学意义(P>0.05);与N-O3组比较,N-GC组血糖升高(P<0.05);与D-O3组比较,D-GC组血糖升高(P<0.05);N-O3组、N-GC组及D-O组、D-GC组的优良率和健康调查简表各项评分比较差异无统计学意义(P>0.05),且未见不良反应发生.结论 侧隐窝注射O3治疗腰椎间盘突出症致神经根炎对糖尿病患者血糖无明显影响. 相似文献
10.
目的:观察颞下颌关节腔注射臭氧治疗颞下颌关节炎的临床疗效。方法:选取2014年6月~2015年6月于我院就诊并被确诊为颞下颌关节炎的患者60例,按照随机原则将所有入选患者分为治疗组30例,对照组30例。治疗组采取颞下颌关节腔注射臭氧治疗,对照组采取颞下颌关节腔注射曲安奈德治疗,治疗之后进行疗效的对比。结果:进行治疗后,治疗组各时点VAS评分及最大张口度与对照组无明显差别。两组情况比较差异无统计学意义。结论:颞下颌关节腔注射臭氧疗效显著,与颞下颌关节腔注射曲安奈德疗效无明显区别,而且并发症少、安全性高。 相似文献