Tannin inhibits HIV-1 entry by targeting gp41

INTRODUCTION Extracts of several Chinese medicinal herbs havebeen shown to have anti-HIV-1 activity, which is correlated with the concentrations of polyphenolic compounds in the extracts[1]. Our previous study has demonstrated thatthe polyphenolic compounds isolated fromtwo antiviral herbs interact with the peptides derivedfrom the HIV-1 gp41, suggesting that the active components in these herbs may inhibit HIV-1 entry by targeting gp41[2]. Tannin (also named tannic acid) is apol…     

血清N末端脑钠肽前体定量检测在心力衰竭中的诊断价值

目的 探讨血清N末端脑钠肽前体定量检测对心力衰竭（heard failure,HF）的诊断价值.方法 使用法国生物梅里埃公司生产的全自动酶免疫荧光分析仪,对105例以呼吸困难为主诉的患者进行血清NT-proBNP检测,比较不同临床诊断分组的NT-proBNP浓度以及不同心功能分级患者的NT-proBNP浓度.结果 无症状心衰组与健康对照组比较NT-proBNP浓度有显著差异,有症状心衰组与无症状心衰组比较差异有显著性;NT-proBNP浓度随着NYHA心功能分级递增而增高,两者呈正相关.结论 NT-proBNP在早期诊断和鉴别心衰有很重要的价值,也是心衰预后评估及危险分层的良好指标.     

The oxime pro-2-PAM provides minimal protection against the CNS effects of the nerve agents sarin,cyclosarin, and VX in guinea pigs

This study examined whether pro-2-PAM, a pro-drug dihydropyridine derivative of the oxime 2-pralidoxime (2-PAM) that can penetrate the brain, could prevent or reverse the central toxic effects of three nerve agents; sarin, cyclosarin, and VX. The first experiment tested whether pro-2-PAM could reactivate guinea pig cholinesterase (ChE) in vivo in central and peripheral tissues inhibited by these nerve agents. Pro-2-PAM produced a dose-dependent reactivation of sarin- or VX-inhibited ChE in both peripheral and brain tissues, but with substantially greater reactivation in peripheral tissues compared to brain. Pro-2-PAM produced 9–25% reactivation of cyclosarin-inhibited ChE in blood, heart, and spinal cord, but no reactivation in brain or muscle tissues. In a second experiment, the ability of pro-2-PAM to block or terminate nerve agent-induced electroencephalographic seizure activity was evaluated. Pro-2-PAM was able to block sarin- or VX-induced seizures (16–33%) over a dose range of 24–32?mg/kg, but was ineffective against cyclosarin-induced seizures. Animals that were protected from seizures showed significantly less weight loss and greater behavioral function 24?h after exposure than those animals that were not protected. Additionally, brains were free from neuropathology when pro-2-PAM prevented seizures. In summary, pro-2-PAM provided modest reactivation of sarin- and VX-inhibited ChE in the brain and periphery, which was reflected by a limited ability to block or terminate seizures elicited by these agents. Pro-2-PAM was able to reactivate blood, heart, and spinal cord ChE inhibited by cyclosarin, but was not effective against cyclosarin-induced seizures.     

心房纤颤与血浆氨基末端脑钠肽前体的相关性

目的 了解血浆氨基末端脑钠肽前体（NT-proBNP）与房颤之间的相关性,并进一步深入探讨阵发性房颤和持续性房颤患者的NT-proBNP水平有无不同.方法 选择150例心脏病患者作为研究对象,其中伴有房颤的患者75例（房颤组）,无房颤患者75例（对照组）,以美国纽约心脏病协会（NYHA）心功能分级标准评价心功能.结果 房颤组NT-proBNP浓度高于对照组.阵发性房颤组患者和持续性房颤组患者NT-proBNP浓度无明显差别.结论 在相同心功能情况下,房颤组NT-proBNP浓度高于对照组;阵发性房颤组患者和持续性房颤组患者NT-proBNP浓度无明显差别.     

儿童急性淋巴细胞白血病细胞诱导前后增殖性抗原的表达研究

用免疫荧光法结合流式细胞术对TPA和rIL-2体外诱导的儿童急性淋巴细胞白血病细胞进行诱导前后的CD25,CD34,CD71,Ki-67和PCNA等细胞增殖性抗原的表达检测,发现CD25和CD71的表达改变不明显,CD34,Ki-67和PCNA在诱导后的白血病细胞中,其表达率明显降低。我们认为TPA和rIL-2能诱导白血病细胞增殖活力的降低,对其增殖性抗原CD34,Ki-67和PCNA的检测是观察白血病细胞向成熟细胞分化的敏感性生物学指标。     

Fetal hypothalamus-pituitary-adrenal axis on the road to parturition

1. Activity of the fetal hypothalamus-pituitary-adrenal (HPA) axis waxes and wanes as a function of gestational age. 2. In a number of species, including sheep, at the end of gestation there is an increase in HPA activity, as characterized by an increase in fetal plasma glucocorticoids. 3. To a certain degree, the hypothalamus, pituitary and adrenal all act autonomously and, therefore, may be thought of as contributing to the initiation of the signal that results in the increase in steroidogenesis before birth. 4. Because it integrates sensory information from beyond as well as within the HPA axis and likely triggers developmental changes within the pituitary, the hypothalamus may be a 'first among equals' in being the ultimate source of triggering information for the HPA axis.     

Changes in sensomotor cortical peptide levels in the rat brain on the appearance of poststimulus paroxysmal discharges

Research Institute of General Pathology and Pathological Physiology, Academy of Medical Sciences of the USSR, Moscow. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 113, No. 2, pp. 118–120, February, 1992.     

热休克蛋白70、90在子宫内膜生长控制中的作用

目的:探讨子宫内膜中热休克蛋白(HSP)70、90表达与雌激素受体(ER)表达的关系及在内膜生长控制中的作用。方法:用免疫组化Envision法和图象分析仪检测30例正常子宫内膜、30例增生过长子宫内膜和53例子宫内膜癌中HSP70、90和ER的表达。结果:增生期内膜中HSP90、ER的表达强于分泌期,在增生过长内膜中的表达进一步增强,而内膜癌中的表达弱于增生期和增生过长内膜;分泌期内膜中HSP70的表达强于增生期内膜,内膜癌中表达较分泌期内膜更强,内膜癌中HSP90表达随病理分级升高而减弱,非子宫内膜样癌中HSP90表达弱于内膜样癌;内膜癌中HSP70表达随病理分级升高而增强,非子宫内膜样癌中HSP70表达强于子宫内膜样癌。子宫内膜中HSP90表达与ER表达呈正相关,HSP70表达与ER表达呈负相关。结论:HSP90可能促进子宫内膜增生,HSP70与子宫内膜的增生、分泌均有关,主要与子宫内膜的分泌有关。内膜癌中HSP70、90表达对癌细胞的生长有重要作用,HSP70对癌细胞的凋亡有一定的保护作用。     

月痛宁滴丸解痉止痛作用的药理研究

目的 研究月痛滴丸对原发性和继发性痛经的影响。方法 观察药物对诱导剂致大、小鼠离体和在体子宫痉挛性痛经、抗炎、抗血栓、血液粘度的影响。结果 月痛宁滴丸能抑制大鼠离体和在体子宫收缩，同等剂量还能抑制大鼠子宫炎性肿胀及小鼠耳壳炎症，降低大鼠;支－静脉血栓重量及全血粘度。结论 月痛宁对原发性与继发性痛经具有明显的抑制作用。