Studies an morphological typing of argyrophilic nucleolar organizer regions

Morphological typing of argyrophilic nucleolar organizer regions (AgNOR) in 300 caws of malignant tumors and 140 cams of benign lesions was analyzed and five morphological types of ApNOR were described in detail. In malignant tumors, the diffuse type (78%) was the most frequently seen, and in benign lesions, the nucleolar type (92.85%); the difference was thus highly significant (P < 0.001). The intranucleolar and aggregate types were not observed in benign lesions. There was no obvious difference in the proportion of the mixed type in benign and malignant lesions (P > 0.05). The relationship between grade of malignancy and morphological typing of AgNOR and its clinical significance are discussed.     

内毒素致家兔氧提取功能障碍的研究

作者复制了家兔病理性氧供依赖性(POSD)模型。采用渐进性低氧法降低总氧运输量(TO_2),从而考察机体氧运输及氧摄取的变化特点。结果表明,在TO_2-VO_2的关系中,对照组可清楚地分为“非依赖”与“依赖”两部分;内毒素组从呼吸空气开始,从未出现坪区,内毒素组和对照组依赖段的斜率,即氧提取率分别为0.473和0.730,两者差别显著(P<0.05)。内毒素组的动静脉血氧含量差值(CaO_2-CVO_2)在渐进性低氧过程中逐渐变小(P<0.01,ANOVA),对照组则呈相反变化趋势,故其组间差别越来越大,表明内毒素组氧提取率降低。     

应用彩色多普勒超声诊断结节性甲状腺病变

目的 探讨彩色多普勒超声鉴别诊断结节性甲状腺病变的意义。方法 回顾性分析病理或细胞学证实的结节性甲状腺病变74例二维超声和彩色多普勒超声（CDFI）表现。结果 结节性甲状腺肿、甲状腺腺瘤和腺癌超声和血流图像表现有一定规律性,定位准确,敏感性强。结论 彩色多普勒超声是诊断结节性甲状腺病变首选检查方法。     

粉防己碱对兔瘢痕组织作用的实验研究

目的 :研究粉防己碱对兔病理性瘢痕的组织学作用 ,为瘢痕治疗提供一种新方法。方法 :采用兔耳腹侧瘢痕模型 ,用粉防己碱 5 0mg /ml、粉防己碱 10mg /ml、去炎松 40mg /ml及生理盐水分别注入瘢痕组织 ,图像分析瘢痕组织中成纤维细胞数量和胶原含量的变化。结果 :粉防己碱治疗组瘢痕组织中成纤维细胞数量与胶原含量均明显减少 ,呈剂量效应关系 ;与生理盐水对照组比较 ,有极显著差异 (P <0 .0 1) ;与去炎松对照组比较无显著差别 (P >0 .0 5 )。结论 :粉防己碱抑制瘢痕组织中成纤维细胞增殖 ,并使胶原合成减少 ,从而抑制瘢痕组织生长 ,有望成为防治病理性瘢痕的新药物     

伴嗜酸性粒细胞增多性血管淋巴样增生的临床病理分析

目的：探讨伴嗜酸性粒细胞增多性血管淋巴样增生的临床病理学特征。方法：对我科1950-1999年期间所诊治的伴嗜酸性粒细胞增多性血管淋巴样增生的7名患者的病理标本重新切片,进行病理学上的分析。结果：伴嗜酸性粒细胞增多性血管淋巴样增生在病理学上有其特征,形态学上的改变主要表现在以下3个方面：①真皮内大量毛细血管增生;②血管内皮细胞增生肿大,似“墓碑”状突入血管腔;③病变处有淋巴细胞、嗜酸性粒细胞混合浸润。结论：伴嗜酸性粒细胞增多性血管淋巴样增生是一种良性局限性血管增生,其病因及发病机制尚不清楚。熟悉这一疾病的组织学改变对避免误诊为其他类拟疾病有重要意义。     

仁术健胃颗粒逆转胃癌前期病变的临床研究

目的观察仁术健胃颗粒对胃癌前病变的逆转作用。方法将１２０例符合诊断标准的胃癌前期病变患者,随机分为仁术健胃颗粒治疗组８０例和胃复春对照组４０例进行临床疗效观察。结果①综合疗效：治疗组总有效率为９１．２５％,对照组为７５．００％,经统计学处理,差异有显著性（Ｐ〈０．０５）;②治疗组胃镜及对萎缩、肠化、异型增生的疗效分别为７１．２５％、７７．５０％、７９．４９％、８８．４６％,对照组为５２．５０％、６     

胎儿脐动脉血流速度指数的临床应用研究

目的：探讨胎儿脐血流S／D值检测的临床意义。方法：采用SRF608型胎儿脐血流检测仪对600例孕妇进行脐血流检查,判定标准为S／D值大于或等于该孕周正常范围的90th%为异常,即S／D≥3．0。结果：妊高征,宫内发育迟缓（IUGR）、脐绕颈、宫内窘迫等病理妊娠中S／D值≥90th%者与正常组比较发生率明显上升,差异非常显著（P＜0．01）。且上述病理妊娠组中S／D比值≥90th%者分娩新生儿发生窒息率明显高于＜90th%者,差异非常显著（P＜0．01）。结论：脐动脉血流波型检测在指导临床处理及评估胎儿预后方面有着重要的作用。     

强的松对大白鼠心肺肝脾肾影响的病理改变

为了解强的松对心肺肝脾肾影响的病理改变,应用正常Wistar大白鼠28只,分实验组及对照组各14只。实验组给予相当于人2mg/kg.d等效剂量的强的松片剂溶入当日饮水中,对照组饮自来水,共10周,之后解剖光镜见对照组心肺肝脾肾及实验组心肺均无异常。实验组肝肾轻度充血,脾充血明显,且见较多舍铁血黄素沉着,以红髓边缘明显。证实常规剂量的强的松对肝脾肾也有一定不良影响,应用激素时要注意及时调整剂量和给药方式与时间,尽量减少副作用。     

The role of the HLA allelic repertoire on the clinical severity of COVID-19 in Canadians,living in the Saskatchewan province

AimsThe HLA system has been implicated as an underlying determinant for modulating the immune response to SARS-CoV-2. In this study, we aimed to determine the association of patients’ HLA genetic profiles with the disease severity of COVID-19 infection.MethodsProspective study was conducted on COVID-19 patients (n = 40) admitted to hospitals in Saskatoon, Canada, between March and December 2020. Next-generation sequencing was performed on the patient samples to obtain high-resolution HLA typing profiles. The statistical association between HLA allelic frequency and disease severity was examined. The disease severity was categorized based on the length of hospital stay and intensive care needs or demise during the hospital stay.ResultsHLA allelic frequencies of the high and low-severity cohorts were normalized against corresponding background allelic frequencies. In the high-severity cohort, A*02:06 (11.8-fold), B*51:01 (2.4-fold), B*15:01(3.1-fold), C*01:02 (3.3-fold), DRB1*08:02 (31.2-fold), DQ*06:09 (11-fold), and DPB1*04:02(4-fold) were significantly overrepresented (p < 0.05) making these deleterious alleles. In the low-severity cohort, A*24:02 (2.8-fold), B*35:01 (2.8-fold), DRB1*04:07 (5.3-fold), and DRB1*08:11 (22-fold) were found to be significantly overrepresented (p < 0.05) making these protective alleles. These above alleles interact with NK cell antiviral activity via the killer immunoglobulin-like receptors (KIR). The high-severity cohort had a higher predilection for HLA alleles associated with KIR subgroups; Bw4-80I (1.1-fold), and C1 (1.6-fold) which promotes NK cell inhibition, while the low-severity cohort had a higher predilection for Bw4-80T (1.6-fold), and C2 (1.6-fold) which promote NK cell activation.ConclusionIn this study, the HLA allelic repository with the distribution of deleterious and protective alleles was found to correlate with the severity of the clinical course in COVID-19. Moreover, the interaction of specific HLA alleles with the KIR-associated subfamily modulates the NK cell-mediated surveillance of SARS-CoV-2. Both deleterious HLA alleles and inhibitory KIR appear prominently in the severe COVID-19 group focusing on the importance of NK cells in the convalescence of COVID-19.