新型饮水对小鼠繁殖及抗氧化酶活性的影响

目的　探讨新型饮水对健康的影响及其保健作用。方法　将矿溶生态水、活化水、纯净水、矿泉水、碱性离子水等 5种新型饮水作为唯一水源分别喂饲亲代及子代小鼠 3个月 ,对照给予天然水。观察它们对小鼠体重、繁殖及抗氧化酶活性的影响。结果　与天然水比较 ,活化水、矿溶生态水促进亲代雄性小鼠体重增长 (P <0 0 5) ;矿溶生态水使子代小鼠、矿泉水和碱性离子水使子代雄性小鼠体重增长缓慢 (P <0 0 5) ;纯净水使新生仔鼠体重减轻 (P <0 0 5) ,活化水、矿溶生态水使小鼠窝产仔数增多 (P <0 0 5) ,5种水对小鼠受孕率、妊娠率、出生存活率无影响 ;矿溶生态水、活化水、纯净水、矿泉水使小鼠肝组织SOD活力升高 (P <0 0 5) ,碱性离子水使肝组织SOD活力降低 (P <0 0 5)。结论　 5种新型饮水对小鼠体重有影响 ,对受孕率、妊娠率、出生存活率无影响 ;矿溶生态水、活化水、纯净水、矿泉水有提高SOD活力作用     

Treatment of traumatized primary teeth: a conservative approach

This retrospective study examined some different types of treatment to primary teeth. The aim of this study was to assess the treatment of traumatized primary teeth and the importance of a long-term follow up. Brazilian children in the age group of 1-4 years from a baby clinic took part in the study. Three hundred and fifteen patients suffered some type of traumatic injury, a total of 338 affected teeth. Data were registered in specific records and submitted to statistic analysis. The most prevalent type of treatment was monitor only (85%) followed by tooth extraction and endodontic procedure. Invasive treatments were performed in case of severe traumas, usually 6 months after the injury. We verified that a careful follow up might be the preferential choice to the treatment of traumatic primary teeth even in some severe cases.     

人皮肤角质形成细胞的分离与原代无血清培养

目的 :建立一种体外人皮肤角质形成细胞分离与原代培养的方法。方法 :采用两步消化法对皮肤进行消化 ,获取角质形成细胞进行体外无血清培养基培养 ,进行细胞形态学观察、免疫组织化学、透射电镜鉴定及生长曲线的绘制与分析。结果 :该方法可获取较多高纯度的角质形成细胞 ,且在体外可快速稳定增殖。结论 :两步消化法和体外无血清培养是一种理想的皮肤角质形成细胞分离培养的方法     

原发性强直性脊柱炎的早期临床特点

目的 探讨原发性强直性脊柱炎（ＡＳ）的早期临床特点。方法 １２０例ＡＳ的早期临床特点。结果 该组病人的发病出现１６－２０岁和３０－３５岁两上发作年龄高山峰，初发年龄较低；外周关节受累的频率较脊柱高；下肢大和骶骼关节常易受累，其中髋关节，膝关节、踝关节和胸锁关节受累的频率分别为７９％，８５％，５７％和６３％，小关节受累少见，虹膜为和主动脉关闭不全的发生率１２．５％和４．５％，未发现有前更炎和类风湿结     

Laparoscopic Findings in Senescent Patients with Primary Biliary Cirrhosis

Abstract: Fifty -two patients with primary biliary cirrhosis (PBC), 10 of whom were 65 years or older at the time of diagnosis, were investigated by laparo-scopy. Laparoscopic findings in these 10 patients were evaluated and compared with those in younger patients. The 10 cases were composed of nine females and one male, and two had been diagnosed as having symptomatic PBC with skin itching, while the remaining eight had asymptomatic PBC. Two, seven and one case were in Scheuer's stage I, II and III, respectively, and eight had chronic non-suppurative destructive cholangitis (CNSDC) on liver biopsy specimens. The majority of senescent PBC patients had typical findings of the early stage of PBC on the liver surface; mild undulations in nine and reddish patches in eight. The laparoscopic findings in senescent PBC were relatively mild.     

成人原发性肾病综合征患者尿视黄醇结合蛋白的测定及意义

采用酶联免疫法检测１００例正常人及７９例成人原发性肾病综合征治疗前后尿视内醇结合蛋白的变化。追踪随访２年，发现经治疗后病理类型为ＥＣＰＧＮ，ＭＣＮ，ＩｇＡ ＧＮ者，尿蛋白及ＲＢＰ均明显减少，其肾功能不全的发生率较低，而ＭＧＮ，ＦＳＧＳ，ＳＧＮ者，尿蛋白及ＲＢＰ无明显减少，肾功能不全的发生率较高，ＭｓＰＧＮ因其增生程度的不同，治疗效果及预后有所不同，轻度增生者治疗效果及预后较好，中，重度增生者，基     

Relationship between cellular ATP content and cellular functions of primary cultured rat hepatocytes in hypoxia

The importance of oxygen in maintaining the functional integrity of hepatocytes has been well established in a variety of experimental models, such as in vivo , perfused liver and isolated hepatocytes. However, one of the shortcomings of these systems is their short life span. Therefore, we have examined the effects of long-term hypoxia on cellular adenine nucleotide content and cellular functions, such as albumin production, urea production and DNA synthesis, in adult rat hepatocytes in primary culture. Hepatocytes were cultured at a density of 11 × 10⁴ and 5 × 10⁴ cells/0.18 mL per cm² for the study of albumin and urea production and DNA synthesis, respectively, at various oxygen tensions (20, 12, 8 and 5%) for 24 h. Cellular ATP content in cultured hepatocytes in hypoxia gradually declined, corresponding to the decrease in oxygen tension, and the cellular ATP level at 5% oxygen was approximately 20% of that at 20% oxygen. Albumin production also decreased in parallel with the decrease in cellular ATP content in cultured hepatocytes in hypoxia. However, even when cellular ATP content gradually declined corresponding with the decrease in oxygen tension in cultured hepatocytes in hypoxia, such as at 8 or 5% oxygen, urea production remained at a high level; in contrast, DNA synthesis was completely suppressed. These results suggest that the cellular ATP content decreases in cultured hepatocytes during long-term hypoxia in relation to oxygen tension and that the relationship between decreased ATP levels and liver function in cultured hepatocytes during hypoxia differs for albumin production, urea production and DNA synthesis.     

115例腹会阴直肠切除术会阴伤口一期缝合的评估

本文就115例腹会阴直肠切除术,对会阴伤口的两种不同处理方法进行分析比较.在年龄、性别、病理分期及恶性程度无明显差异的情况下,会阴伤口一期缝合组的平均愈合时间为16.90±4.35天,伤口敞开组为37.04±15.44天,差异显著(P<0.002).术后局部复发率分别为3/69例和2/46例,均为4.35%(P>0.05).一期缝合组的平均住院费用约为敞开组的46.87%～76.52%.作者指出,鉴于直肠癌逆行向下的淋巴扩散极为罕见,仅发生于高恶性或伴广泛淋巴转移的病例,因而对癌肿远端需切除2cm～3cm正常肠管而同时切除肛直肠环、不得不作手术者,会阴部清除范围无需太大,会阴伤口可分层完全缝合;对位于肛管内或侵及肛直肠环的癌肿,须彻底清除坐骨直肠窝脂肪结缔组织.本文两组术后局部复发率无差异,进一步表明这一认识的正确性.本组结果充分表明了会阴伤口一期缝合法的合理性与可行性.     

新形势下中学与大学学生党建衔接工作的思考

在青年学生中吸收优秀分子已成为高校党组织发展工作的重要组成部分,最近几年来,高中党建工作也不断发展。了解青年学生党建现状,更好地利用中学党建工作成果,提出了做好衔接工作的现实意义及对策措施。