ANXA1，Ki-67抗原在宫颈上皮内瘤变和宫颈癌中的表达及其相互关系

目的：探讨膜联蛋白A1（Annexin A1,ANXA1）和细胞核相关抗原Ki-67在正常宫颈上皮、宫颈上皮内瘤变和宫颈鳞状细胞癌组织中的表达及其相关性。方法：应用免疫组化法检测56例宫颈上皮内瘤变（ CIN）,39例早期宫颈鳞状细胞癌（ SCC）和26例正常宫颈鳞状上皮组织中ANXA1和Ki-67的表达,分析两者之间表达的相关性。应用免疫印迹法检测正常宫颈组织、宫颈上皮内瘤变及宫颈鳞状细胞癌组织中ANXA1的蛋白表达情况。结果：在正常宫颈上皮中 ANXA1蛋白呈高阳性表达,随着宫颈病变的进展, ANXA1蛋白表达呈明显下降趋势。在正常宫颈上皮、宫颈上皮内瘤变和宫颈癌组织中ANXA1蛋白表达具有显著性差异（ P＜0.05）。在正常宫颈上皮和CIN组织中Ki-67抗原表达与ANXA1蛋白表达呈负相关（ P＜0.01）。结论：ANXA1蛋白在宫颈癌中低表达,并与抗原表达明显负相关,与宫颈癌发生、发展过程中凋亡抑制和增殖能力增强有关,可以作为预测宫颈鳞癌不良预后因素之一。     

经阴道超声监测孕妇宫颈形态预测早产的价值

目的评价经阴道超声监测宫颈形态变化对早产的预测价值。方法 223例孕妇分为足月分娩组134例和先兆早产组89例,分别于孕24、28、32及36周行经阴道超声检查,观察宫颈形态长度的变化,以及宫颈指数,并对其妊娠结局进行随访。结果足月分娩组中宫颈长度随孕周增大而缩短;先兆早产组孕妇的宫颈长度均较相应孕周的足月分娩组短,其中24、28及36周组间比较,差异有统计学意义(P<0.05)。先兆早产组各孕周的宫颈指数均较足月分娩组大,两组比较差异有统计学意义(P<0.05)。89例先兆早产组中71例保胎成功,18例发生早产。先兆早产组中21例有宫颈漏斗形成,18例早产者均有宫颈漏斗形成。漏斗形成预测早产的敏感性为100%、特异性为96.6%;以足月分娩孕妇28周时的宫颈长度2.61cm为临界值,其预测早产的敏感性为83.3%、特异性为100%。结论经阴道超声实时监测宫颈形态结构变化对预测早产的发生有一定的临床价值。     

鳞状上皮细胞癌抗原监测宫颈鳞癌的临床价值

 目的　探讨鳞状上皮细胞癌抗原在监测宫颈鳞癌中的临床价值。方法　用微粒子酶免分析技术对 116例宫颈鳞癌治疗前后进行SCC测定。结果　SCC在宫颈鳞癌中的敏感性为 73.2 8% ,特异性为95 .10 %。SCC阳性率在宫颈鳞癌大细胞角化型及非角化型中较高 ,在小细胞型中较低。并且随着宫颈癌临床分期的升高而增加。在根治性治疗后SCC阳性者均转阴性。结论　SCC是宫颈鳞癌较好的一种肿瘤标记物。在宫颈鳞癌的辅助诊断 ,治疗效果的判断 ,监测疾病状态中有一定价值。     

子宫颈支原体感染与胚胎停止发育的关系

目的:探讨子宫颈支原体感染与胚胎停止发育的关系.方法:病例选取起止时间为2018年7月至2019年12月,入组200例病例均为本院收治的流产患者.对其临床资料进行回顾性分析,将发生子宫颈支原体感染患者纳入研究组(100例),将未发生感染患者纳入对比组(100例).对比分析两组患者发生胚胎停止发育的情况.结果:组间对比,...     

子宫颈小细胞神经内分泌癌临床病理分析

目的探讨宫颈小细胞神经内分泌癌(small cell neuroendocrine carcinoma of the cervix,SCNEC)的临床病理特征和免疫组化特点。方法对四川省妇幼保健院病理科和四川省人民医院病理科2006年1月至2014年6月诊治的8例宫颈小细胞神经内分泌癌进行组织形态学、免疫组化观察,并对其临床资料进行整理分析。结果 8例患者平均年龄45.6岁,均以阴道不规则出血或宫颈接触性出血就诊。组织学显示肿瘤由大小较为一致的圆形、卵圆形细胞构成。其中单纯性小细胞神经内分泌癌7例,合并腺癌成分1例。免疫组化:所有病例癌细胞细胞角蛋白(CK)不同程度阳性,且均有两种以上神经内分泌标记阳性,其中6例突触素(Syn)和嗜铬素(Cg A)均阳性,4例神经细胞黏附分子(CD56)阳性。随访7例患者,死亡3例。结论宫颈小细胞癌作为一种少见的高度恶性肿瘤,进展快,预后差,免疫组化神经内分泌标志阳性有助于诊断。     

Degradation of cartilage by macrophages in culture: Evidence for the involvement of an enzyme which is associated with the cell surface

A cell culture system is described in which purified mononuclear phagocytes may be cultured with a cartilage substrate which is radiolabelled in its proteoglycan. Resident mouse peritoneal macrophages degraded this substrate, and did so more avidly if cultured in direct contact with it. There was no evidence for complete intralysosomal degradation of the proteoglycan of the cartilage. Lysates were found to contain considerable activity at pH 7, which was inhibited by the presence of 10% serum, or by boiling the lysate.

Proximity of macrophages to the substrate did not induce selective release of the lysosomal marker enzyme hexosaminidase, and concentrated enzymes secreted from the macrophages after treatment with the lysosomotropic agent ammonium chloride were ineffective in degrading cartilage at neutral pH.

The active enzyme in macrophage lysates at neutral pH was found to be sedimentable by 100,000 × g centrifugation for 1 hour, in absence of lysosomal protective agents. There is evidence for a cell membrane-associated process in the degradation of cartilage by these cells, which may be a proteolytic, endoglycosidic or free radical-mediated event.     

印迹基因IGF2与p53在子宫颈鳞癌、子宫颈上皮内瘤变及子宫颈炎中的表达及意义

目的：探讨IGF2与p53在子宫颈鳞癌、子宫颈上皮内瘤变（cervical intraepithelial neoplasia, CIN）及子宫颈炎中的表达及意义。方法采用免疫组化EnVision两步法检测IGF2与p53在45例子宫颈炎、126例CIN、47例子宫颈鳞癌组织中的表达,分析其与临床病理参数间的关系。结果子宫颈鳞癌组织中IGF2阳性表达显著高于CIN 及子宫颈炎（P<0.05）;p53阳性表达显著高于CIN 及子宫颈炎（P<0.0001）。子宫颈鳞癌组织中IGF2的阳性表达与浸润深度以及淋巴结转移呈负相关（P<0.05）。结论 IGF2、p53的异常表达与子宫颈鳞癌的发生密切相关。     

Necrotizing small-vessel vasculitis confined to the uterine cervix

OBJECTIVES: To report our experience with five cases of apparently isolated small-vessel vasculitis of the uterine cervix. METHODS: Case study of five patients with necrotizing vasculitis discovered incidentally in surgical specimens of the female genital tract, and a review of the pertinent literature on this subject. RESULTS: All patients lacked clinical and serological features of the well-delineated vasculitic syndromes. Comprehensive workup failed to yield any evidence of an underlying disorder. All patients were managed expectantly and did not develop systemic vasculitis during follow-up ranging from 6 months to 5 years. CONCLUSIONS: Isolated vasculitis of the female genital tract can be encountered as an innocuous finding in otherwise healthy individuals. The cause and pathogenesis of this disorder remain obscure. Rheumatologists should be familiar with this rare and vexing form of vasculitis and with its benign prognosis.     

Oncostatin M receptor is a novel therapeutic target in cervical squamous cell carcinoma

Cervical carcinoma is the second most common cause of cancer deaths in women worldwide. Treatments have not changed for decades and survival rates for advanced disease remain low. An exciting new molecular target for the treatment of cervical squamous cell carcinoma (SCC), and possibly for SCCs at other anatomical sites, is the oncostatin M receptor (OSMR). This cell surface cytokine receptor is commonly copy number gained and overexpressed in advanced cervical SCC, changes that are associated with significantly worse clinical outcomes. OSMR overexpression in cervical SCC cells results in enhanced responsiveness to the major ligand oncostatin M (OSM), which induces several pro‐malignant effects, including a pro‐angiogenic phenotype and increased cell migration and invasiveness. OSMR is a strong candidate for antibody‐mediated inhibition, a strategy that has had a major impact on haematological malignancies and various solid tumours such as HER2‐positive breast cancers. © 2013 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.