E-cadherin基因蛋白在口腔疣状癌中的表达

目的 :研究E 钙粘素 (E cadherin)基因蛋白表达与口腔疣状癌发生发展的相关性。方法 :利用免疫组织化学SP法检测 1 2例口腔疣状癌、2 0例口腔鳞癌组织中的E cadherin基因蛋白表达 ,对比分析其相关性。结果 :口腔疣状癌中的E cadherin基因蛋白阳性表达率为 83.3% ,明显高于口腔低分化鳞癌 (37.5 % ;P <0 .0 5 ) ;其阴性表达率介于高分化鳞癌与低分化鳞癌之间。结论 :E cadherin基因蛋白在口腔疣状癌的发生及临床表型中起重要作用     

FOXQ1和E-cadherin在食管鳞状细胞癌中的表达及临床意义

目的探讨叉头框蛋白Q1(FOXQ1)和E-钙黏蛋白(E-cadherin)在食管鳞状细胞癌(ESCC)中的表达及临床意义。方法采用免疫组织化学法检测42例ESCC组织(ESCC组)及其癌旁正常食管鳞状上皮组织(正常组)中FOXQ1和E-cadherin的表达情况,分析其表达与患者临床病理特征及预后的关系。结果 ESCC组FOXQ1的阳性表达率高于正常组(64.29%vs 28.57%,χ2=5.384,P<0.05),E-cadherin的阳性表达率低于正常组(52.38%vs 90.48%,χ2=7.691,P<0.05)。FOXQ1的表达强度在不同TNM分期及不同淋巴结转移情况间的差异有统计学意义,E-cad-herin的表达强度在不同浸润深度、分化程度、TNM分期和淋巴结转移情况间的差异有统计学意义。ESCC组织中FOXQ1和E-cadherin的表达呈负相关(r=-0.412,P<0.05)。FOXQ1高表达者的5年生存率低于低表达者(18.52%vs 66.67%,χ2=9.737,P<0.05),E-cadherin高表达者的5年生存率高于低表达者(59.09%vs 10.00%,χ2=10.996,P<0.05)。COX比例风险回归模型分析结果显示,FOXQ1高表达、E-cadherin低表达、有淋巴结转移是影响ESCC患者预后的独立危险因素。结论 FOXQ1和E-cadherin在ESCC组织中的表达呈现较好的相关性,联合检测两者的表达对于ESCC患者的预后判断及指导治疗具有一定的临床价值。     

E-cadherinP120ctn在乳腺癌中的表达及意义

  目的  探讨E-钙黏蛋白(E-cadherin, E-cad)、P120连环蛋白(P120ctn)在乳腺浸润性导管癌(IDC)和浸润性小叶癌(ILC)中的表达及其鉴别诊断意义。  方法  收集解放军第113医院2007年12月至2012年5月间有完整随访资料的浸润性导管癌60例、浸润性小叶癌48例及混合性癌20例。采用免疫组织化学S-P法检测E-cad及P120的表达。  结果  E-cad在IDC和ILC中的阳性率分别为85%(51/60)和0, 其差异有统计学意义(P < 0.01);P120在IDC中阳性率为100%, 且均为细胞膜着色, 而在ILC中阳性率亦为100%, 但均为胞浆着色。E-cad和P120联合使用将20例混合癌确诊为IDC 16例及ILC 4例。IDC中E-cad的表达水平与肿瘤分期无关, 与淋巴结转移有关(P < 0.05)。  结论  E-cad和P120联合使用可以鉴别光镜下易混淆的IDC和ILC, 使组织分型更准确, 建议应用于乳腺癌的常规免疫组织化学检测。E-cad的表达水平与淋巴结转移有关。      

E-cadherin、α-catenin、β-catenin在膀胱肿瘤中的表达及其意义

目的 探讨E-cadherin(E-cad)、α-catenin(α-cat)、β-catenin(β-cat)复合体在膀胱移行细胞癌中的表达及意义。方法 用S-P免疫组化方法检测54例膀胱移行细胞癌中E-cad、α-cat、β-cat基因蛋白的表达，综合分析其表达与膀胱移行细胞癌病理分级、分期的关系。结果 在膀胱移行细胞癌中，E-cad、α-cat、β-cat正常表达率分别为29．6％、18．5％、25．9％。三者的表达与肿瘤的分级、分期呈负相关(P＜0．05)。高分化的病例三者组合阳性率低于低分化或未分化的病例(P＜0．05)。结论 E-cad、α-cat、β-cat三者同时测定对膀胱移行细胞癌预后有重要意义。     

钙粘附蛋白在妇科肿瘤中的研究新进展

钙粘附蛋白是一组钙离子依赖性的跨膜糖蛋白 ,是组织特异性的细胞间连接分子 ,在组织分化及维持组织完整性中起重要作用。钙粘附蛋白家族的异常表达或缺失是肿瘤发生和发展的因素之一 ,该文着重对钙粘附蛋白家族在某些妇科肿瘤的发生、转移及预后中的作用的研究进展做以综述     

Sleeping Giants: Emerging Roles for the Fat Cadherins in Health and Disease

The vertebrate Fat cadherins comprise a small gene family of four members, Fat1–Fat4, all closely related in structure to Drosophila ft and ft2. Over the past decade, knock‐out mouse studies, genetic manipulation, and large sequencing projects has aided our understanding of the function of vertebrate Fat cadherins in tissue development and disease. The majority of studies of this family have focused on Fat1, with evidence now showing it can bind enable (ENA)/Vasodilator‐stimulated phosphoprotein (VASP), β‐catenin and Atrophin proteins to influence cell polarity and motility; HOMER‐1 and HOMER‐3 proteins to regulate actin accumulation in neuronal synapses; and scribble to influence the Hippo signaling pathway. Fat2 and Fat3 can regulate cell migration in a tissue specific manner and Fat4 appears to influence both planar cell polarity and Hippo signaling recapitulating the activity of Drosophila ft. Knowledge about the exact downstream signaling pathways activated by each family member remains in its infancy, but it is becoming clearer that they have tissue specific and redundant roles in development and may be lost or gained in cancer. In this review, we summarize the recent progress on understanding the role of the Fat cadherin family, integrating the current knowledge of molecular interactions and tissue distributions, together with the accumulating evidence of their changed expression in human disease. The latter is now beginning to promote interest in these molecules as both biomarkers and new targets for therapeutic intervention.     

胃癌组织中maspin基因和HGF及E-cadherin的表达及相关性研究

目的:研究胃癌组织中maspin基因、HGF和E-cadherin的表达及相关关系。方法:应用免疫组化ABC法检测137例胃癌、66例不典型增生及54例正常胃黏膜组织中maspin基因、HGF和E-cadherin的表达。结果:在正常胃黏膜、不典型增生和胃癌组织中maspin基因表达分别为72·2%(39/54)、63·6%(42/66)和40·9%(56/137),差异有统计学意义,P<0·05;HGF表达分别是22·2%(12/54)、40·9%(27/66)和67·9%(93/137),差异有统计学意义,P<0·05;E-cadherin表达分别是88·9%(48/54)、77·3%(51/66)和52·5%(72/137),差异有统计学意义,P<0·05。maspin基因的表达与淋巴结转移、浸润深度及分化程度有关,与肿瘤大小、病理分型及TNM分期无关。HGF的表达与TNM分期、淋巴结转移相关,与病理分型、肿瘤大小、分化程度和浸润深度无关。E-cadherin表达与浸润深度、分化程度、淋巴结转移和TNM分期相关,与病理分型、肿瘤大小无关。Maspin基因表达与HGF呈负相关,r=-0·350,P<0·01;与E-cadherin呈正相关,r=0·433,P<0·01。结论:maspin基因在胃癌组织中表达下调可能导致细胞黏附减少及细胞的运动能力增加引起胃癌的发生发展和侵袭转移。     

Osteolytic bone metastasis in breast cancer

Summary Metastasis of breast cancer cells to bone consists of multiple sequential steps. To accomplish the process of metastasis to bone, breast cancer cells are required to intrinsically possess or acquire the capacities that are necessary for them to proliferate, invade, migrate, survive, and ultimately arrest in bone. These capacities are essential for any cancer cells to develop distant metastases in organs such as lungs and liver as well as bone. Once breast cancer cells arrest in bone, bone is a storehouse of a variety of cytokines and growth factors and thus provides an extremely fertile environment for the cells to grow. However, breast cancer cells are unable to progress in bone unless they destroy bone with the assistance of bone-resorbing osteoclasts. Thus, the capacity of breast cancer cells to collaborate with osteoclasts is likely to be specific and is likely critical for them to cause osteolytic bone metastases. Evidence to support the concept that there is an intimate relationship between breast cancer cells and osteoclasts is described using anin vivo bone metastasis model in which human breast cancer cells are inoculated into the left ventricle of nude mice. The roles of cell adhesion molecules including cadherins and laminin and matrix metalloproteinases in the development of osteolytic bone metastases by breast cancer are also discussed.     

食管鳞状细胞癌的PTTG1表达及与上皮间质转化的关系及临床意义

目的 分析食管鳞状细胞癌（ESCC）的垂体瘤转化基因1（PTTG1）表达及与上皮间质转化（EMT）的关系及临床意义。方法 选取2015年1月—2017年12月山东枣庄矿业集团中心医院接受手术治疗的73例经病理确诊的ESCC患者的手术标本,取癌旁组织作为对照。采用免疫组织化学法检测组织中E-钙黏附素、波形蛋白、N-钙黏附素及PTTG1表达,采用RNA干扰技术下调ESCC Eca109细胞系中PTTG1表达,采用Western blotting检测下调ESCC细胞系中E-钙黏附素、波形蛋白、N-钙黏附素及PTTG1表达,采用RT-PCR 检测下调ESCC Eca109细胞系中E-钙黏附素、波形蛋白、N-钙黏附素及PTTG1信使RNA（mRNA）表达。结果 与癌旁组织比较,癌组织的波形蛋白、N-钙黏附素及PTTG1阳性表达高（P?<0.05）,E-钙黏附素阳性表达低（P?<0.05）。E-钙黏附素、波形蛋白、N-钙黏附素及PTTG1表达与ESCC患者的浸润深度、淋巴结转移、肝转移及分期相关（P?<0.05）。PTTG1表达还与ESCC患者的分化程度相关（P?<0.05）。PTTG1表达下调ESCC Eca109细胞系中E-钙黏附素蛋白及E-钙黏附素mRNA增高（P?<0.05）,波形蛋白、N-钙黏附素、PTTG1蛋白及波形蛋白、N-钙黏附素、PTTG1 mRNA均降低（P?<0.05）。结论 ESCC肿瘤组织中存在PTTG1高表达,其表达与肿瘤的EMT相关。