溃疡型浅表淋巴结结核50例临床分析

目的治疗组和对照组观察溃疡型淋巴结结核的临床疗效。方法随机抽样将溃疡型淋巴结结核的病人分为治疗组和对照组。治疗组采用清创＋INH＋RFP外敷;对照组采用清创＋RFP外敷。结果治疗组和对照组病人伤口的愈合时间分别为15—70d和17～74d,差异无统计学意义（P〉0．05）。结论伤口的愈合与清创是否彻底关系密切,不受局部单用RFP或联合用药的影响。     

Imaging the inhibition by anti-β1 integrin antibody of lung seeding of single osteosarcoma cells in live mice

Integrins play a role in tumor growth and metastasis. However, the effect of integrin inhibition has not been visualized on single cancer cells in vivo. In this study, we used a powerful subcellular in vivo imaging model to demonstrate how an anti-integrin antibody affects seeding and growth of osteosarcoma cells on the lung. The 143B human osteosarcoma cell line, expressing red fluorescent protein (RFP) in the cytoplasm and green fluorescent protein (GFP) in the nucleus, was established. Such double-labeled cells enable imaging of apoptosis and mitosis and other nuclear-cytoplasmic dynamics. Using the double-labeled osteosarcoma cells, single cancer-cell seeding in the lung after i.v. injection of osteosarcoma cells was imaged. The anti-β1 integrin monoclonal antibody, AIIB2, greatly inhibited the seeding of cancer cells on the lung (experimental metastasis) while a control antibody had no effect. To image the efficacy of the anti-integrin antibody on spontaneous metastasis, mice with orthotopically-growing 143B-RFP cells in the tibia were also treated with AIIB2 or control anti-rat IgG1 antibody. After 3 weeks treatment, mice were sacrificed and primary tumors and lung metastases were evaluated with fluorescence imaging. AIIB2 significantly inhibited spontaneous lung metastasis but not primary tumor growth, possibly due to inhibition of lung seeding of the cancer cells as imaged in the experimental metastasis study. AIIB2 treatment also increased survival of mice with orthotopically growing 143B-RFP.     

Tumor-targeting Salmonella typhimurium A1-R inhibits human prostate cancer experimental bone metastasis in mouse models

Bone metastasis is a frequent occurrence in prostate cancer patients and often is lethal. Zoledronic acid (ZOL) is often used for bone metastasis with limited efficacy. More effective models and treatment methods are required to improve the outcome of prostate cancer patients. In the present study, the effects of tumor-targeting Salmonella typhimurium A1-R were analyzed in vitro and in vivo on prostate cancer cells and experimental bone metastasis. Both ZOL and S. typhimurium A1-R inhibited the growth of PC-3 cells expressing red fluorescent protien in vitro. To investigate the efficacy of S. typhimurium A1-R on prostate cancer experimental bone metastasis, we established models of both early and advanced stage bone metastasis. The mice were treated with ZOL, S. typhimurium A1-R, and combination therapy of both ZOL and S. typhimurium A1-R. ZOL and S. typhimurium A1-R inhibited the growth of solitary bone metastases. S. typhimurium A1-R treatment significantly decreased bone metastasis and delayed the appearance of PC-3 bone metastases of multiple mouse models. Additionally, S. typhimurium A1-R treatment significantly improved the overall survival of the mice with multiple bone metastases. The results of the present study indicate that S. typhimurium A1-R is useful to prevent and inhibit prostate cancer bone metastasis and has potential for future clinical use in the adjuvant setting.     

Comparison of the selective targeting efficacy of Salmonella typhimurium A1-R and VNP20009 on the Lewis lung carcinoma in nude mice

Salmonella typhimurium A1-R is auxotrophic for arg and leu, which attenuates growth in normal tissue but allows high tumor targeting and virulence. A1-R is effective against metastatic human prostate, breast, and pancreatic cancer as well as osteosarcoma, fibrosarcoma, and glioma in clinically-relevant mouse models. VNP20009 is also a genetically-modified strain of Salmonella typhimurium that has been tested in Phase I clinical trials, but is more attenuated than S. typhimurium A1-R and in addition of multiple amino-acid auxotrophs, is purine auxotropic with the purI mutation. In the present study, mouse Lewis lung carcinoma-bearing nude mouse models were treated with S. typhimurium A1-R or VNP20009. S. typhimurium A1-R and VNP20009 were both eliminated from the liver and spleen approximately 3-5 days after administration via the tail vein. However, A1-R showed higher tumor targeting and inhibited the Lewis lung carcinoma to a greater extent than VNP20009, with less body weight loss. The mice tolerated S. typhimurium A1-R to at a least 2-fold higher dose than VNP20009 when the bacteria were administered iv. The results of the present study suggest that S. typhimurium A1-R has greater clinical potential than VNP20009.     

PPH在直肠肛门疾病治疗中的应用及操作要点

目的将PPH运用于直肠肛门疾病治疗并完善手术操作技巧。方法对68例IRP患者(其中65例合并有内痔、混合痔),41例RFP患者(其中39例合并有内痔、混合痔),93例单纯内痔、混合痔患者采用PPH进行手术治疗的临床资料作回顾性分析。结果随访6个月至3年,主述症状完全改善或基本改善者197例,部分改善者5例。结论运用PPH治疗IRP(或合并有混合痔、内痔)、RFP(或合并混合痔、内痔)以及单纯混合痔、内痔患者效果良好。在手术时间、操作方法、术后恢复等方面优于非的手术治疗。     

Moving epithelia: Tracking the fate of mammalian limbal epithelial stem cells

Lineage tracing allows the destiny of a stem cell (SC) and its progeny to be followed through time. In order to track their long-term fate, SC must be permanently marked to discern their distribution, division, displacement and differentiation. This information is essential for unravelling the mysteries that govern their replenishing activity while they remain anchored within their niche microenvironment. Modern-day lineage tracing uses inducible genetic recombination to illuminate cells within embryonic, newborn and adult tissues, and the advent of powerful high-resolution microscopy has enabled the behaviour of labelled cells to be monitored in real-time in a living organism. The simple structural organization of the mammalian cornea, including its accessibility and transparency, renders it the ideal tissue to study SC fate using lineage tracing assisted by non-invasive intravital microscopy. Despite more than a century of research devoted to understanding how this tissue is maintained and repaired, many limitations and controversies continue to plague the field, including uncertainties about the specificity of current SC markers, the number of SC within the cornea, their mode of division, their location, and importantly the signals that dictate cell migration. This communication will highlight historical discoveries as well as recent developments in the corneal SC field; more specifically how the progeny of these cells are mobilised to replenish this dynamic tissue during steady-state, disease and transplantation. Also discussed is how insights gleaned from animal studies can be used to advance our knowledge of the fundamental mechanisms that govern modelling and remodelling of the human cornea in health and disease.     

高效液相色谱法测定复合制剂中异烟肼和利福平含量

抗结核药物的复合制剂有其突出的优点。其含量的测定繁杂费时，需要逐个药物分别测定。本文报告一种可以同时测定异烟肼和利福平两种药物的高效液相色谱法。本法用Ｓｐｈｅｒｉｓｏｒｂ　Ｃ８（４．６×２５０ｍｍ，５μｍ，ＩＤ）色谱柱，以甲醇：０．０５ＭＮａＡｃ／ＨＡＣ缓冲液（ｐＨ６．９０）＝６５：３５（Ｖ：Ｖ）为流动相，流速１．０ｍｌ／ｍｉｎ，紫外检测器检测（＝２５４ｎｍ）。结果：异烟肼和利福平的保留时间分别为３．０１１ｍｉｎ和６．７９ｍｉｎ，在１．０～２０．０μｇ／ｍｌ浓度范围内线性关系分别为：Ｙ＝２０１７．４０Ｘ－３３９．９５，ｒ＝０．９９９７；Ｙ＝２１００．７０Ｘ＋１２５．７６，ｒ＝０．９９９５，日内变异分别为 １．８３％、２．０８％；日间差异分别为 １．８９％、２．１７％。回收率分别为 ９９．７２％、９８．２７％。本法快速，可同时测定两种药物。     

Prognosis of chronic pulmonary aspergillosis in patients with pulmonary non-tuberculous mycobacterial disease

Background

Pulmonary non-tuberculous mycobacterial disease (PNTM) is a known risk factor for chronic pulmonary aspergillosis (CPA). However, few studies have focused on the prognosis of PNTM-associated CPA. In the present investigation, we aimed to elucidate the clinical course and prognostic factors of CPA in patients with PNTM.

局部植入利福平(RFP)缓释剂对P糖蛋白(P-gp)活性及激素性股骨头坏死的影响

 目的  观察局部植入利福平(rifampicin,RFP)缓释剂对P糖蛋白(P-glycoprotein,P-gp)活性及激素性股骨头坏死的影响。方法  建立激素性股骨头坏死的动物模型,取32只成年雌性兔,随机平均分为4组:3个对照组(空白/口服给药/肌肉注射)和局部缓释组(试验组)。其中局部缓释组于左侧股骨上段植入RFP缓释剂,右侧相同位置植入空白颗粒作为对照。4周后检测外周血、骨髓内单核细胞P-gp活性,肝内细胞色素P450 (cytochrom P450,CYP450)含量,比较骨代谢血清学指标、组织学形态(HE、Masson染色)、股骨头坏死率。结果  局部缓释组左侧骨髓内P-gp活性高于右侧(P<0.05)。口服、肌注组外周血P-gp活性和肝细胞色素P4503A含量高于局部缓释组(P<0.05)。HE染色示:局部缓释组左侧股骨头比右侧骨小梁增宽,脂肪细胞减少,骨坏死率降低(P<0.05);口服、肌注组股骨头骨质流失及脂肪化受到抑制。Massons染色示:口服、肌注及局部缓释组左侧股骨头骨质成熟胶原较多,矿化完全。结论  局部植入RFP对外周P-gp及肝内CYP450无明显影响,可增强骨内P-gp活性,降低激素性骨坏死发生率。