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41.
白细胞介素-8研究进展 总被引:14,自引:0,他引:14
白细胞介素-8(IL-8)在趋化性细胞因子家族中属于C-X-C亚家族,IL-8是一个多种细胞来源的趋化性细胞因子,而且不同类型的细胞对不同的诱导剂的反应也不相同,因为由不同的实验室从不同的来源得到该因子,所以对于IL-8的许多不同的描述术语,对于IL-8的生理生化特性,目前已基本清楚,对于IL-8基因表达的调节,现认为主要是在转录水平的调控,并且发现其基因5′-侧翼区的AP-1,NF-кB及NF-IL-6结合位点是调节IL-8启动子功能的主要顺式作用元件,IL-8基因的表达需要这些元件之间高度的协同以达到有效的转录,IL-8受体则是一个由59KDa和67KDa亚单位组成的二聚体糖蛋白,存在于多种类型的细胞上,甚至包括一些不表达IL-8的细胞,IL-8的生物学活性并不表现出种族的特异性,IL-8可激活中性粒细胞,对T-淋巴细胞有趋化性并能刺激嗜碱性粒细胞,内皮细胞IL-8基因的表达除了受化学因子的调节外还受力学因素的影响,流体切应力诱导内皮细胞表达IL-8,可能在急性炎症和动脉粥样硬化的发生,发展过程中具有重要作用。 相似文献
42.
Alyce C. Russell Agnieszka Kepka Irena Trbojević-Akmačić Ivo Ugrina Manshu Song Jennie Hui Michael Hunter Simon M. Laws Gordan Lauc Wei Wang 《Immunobiology》2019,224(1):110-115
Background
Increased body fat may be associated with an increased risk of developing an underlying pro-inflammatory state, thus leading to greater risk of developing certain chronic conditions. Immunoglobulin G has the ability to exert both anti- and pro-inflammatory effects, and the N-glycosylation of the fragment crystallisable portion is involved in mediating this process. Body mass index, a rudimentary yet gold standard indication for body fat, has been shown to be associated with agalactosylated immunoglobulin G N-glycans.Aim
We aimed to determine the association between increased body fat and the immunoglobulin G glycosylation features, comparing body mass index to other measures of body fat distribution.Methods
We investigated a sample of 637 community-based 45–69?year olds, with mixed phenotypes, residing in Busselton, Western Australia. Body mass index and the waist-to-hip and waist-to-height ratios were calculated using anthropometry, while dual-energy x-ray absorptiometry was performed to gain an accurate measure of total and area specific body fat. Serum immunoglobulin GN-glycans were analysed by ultra-performance liquid chromatography.Results
Twenty-two N-glycan peaks were found to be associated with at least one of the fat measures. While the previous association of body mass index to agalactosylated immunoglobulin G was replicated, measures of central adiposity explained the most variation in the immunoglobulin G glycome.Conclusion
Central adiposity is associated with an increased pro-inflammatory fraction of immunoglobulin G, suggesting that the android/gynoid ratio or waist-to-height ratio instead be considered when controlling for adiposity in immunoglobulin G glycome biomarker studies. 相似文献43.
A simple visual observation system, supplemented by measurement of skin potential, was devised for developmental studies of sleep cycles in settings where multiple electrode placement is not practicable. The findings replicated essential features of quiet and active sleep cycles which had been reported previously to exist against the background of decreasing level of physiological arousal, as sleep proceeds. Twelve newborns showed approximately one-half of their inter-feeding sleeping time in the rapid eye movement stage of sleep. Skin potential rapidly declined from the waking level, continued to decrease in level throughout sleep, increased in variability during REM sleep, and increased in level at the second waking period. 相似文献
44.
In the event of smallpox bioterrorism, widespread vaccination may be required. Vaccinia immune globulin (VIG) has been used to treat complications from the smallpox vaccine. While the potency of VIG was defined by its ability to neutralize intracellular mature virus, a second form of vaccinia called the extracellular enveloped virus (EEV) is critical for virus spread in the host. The B5R-protein is one of many EEV-specific proteins. Immunoprecipitation and ELISA revealed that VIG recognizes the B5R-protein. An EEV plaque-reduction assay using a recombinant vaccinia that lacks the majority of the extracellular domain of B5R showed that the ability of VIG to neutralize EEV is principally directed at B5R. In addition, absorbing out the anti-B5R antibody present in VIG through the addition of recombinant B5R protein abrogated VIG's ability to significantly neutralize wild-type EEV. This work demonstrates the prominent role of B5R as a target of EEV-neutralizing activity of human antibodies. 相似文献
45.
46.
Hozumi Katsuto; Kondo Motonari; Nozaki Hideki; Kobori Akiko; Nishimura Takashi; Nishikawa Shin-Ichi; Sugamura Kazuo; Habu Sonoko 《International immunology》1994,6(9):1451-1454
The effects of IL-7 on the growth and differentiation of thymocyteswere analyzed using murine fetal thymua organ cultures (FTOC)in the presence of mAbs specific for the conventional IL-7 receptor(1L-7R) and for the common (c) chain. In FTOC, the developmentof CD4–CD8– double-negative thymocytes to CD4+CD8+double-positive (DP) and CD4+ or CD8+ single-positive (SP) cellswas not completely blocked by adding these mAbs, although cellgrowth was reduced by the treatment. To define a developingstage sensitive to the mAbs, most immature thymocytes, Pgp-1+c-kit cells, were cultured in the 2-deoxyguartosine treatedfetal thymus. In the presence of both mAbs in the culture, neitherDP nor SP thymocytes developed whereas either of the mAbs partiallyblocked their development. These results indicate that the Cchain is involved in early T cell development as an indispensablesubunlt of the functional IL-7 receptor complex. 相似文献
47.
48.
Rabbit retinal Müller cells were isolated by means of papaine and mechanical dissociation. These cells were shown to have a well preserved morphology and to preserve viability for many hours. Intense wheat germ agglutinin binding occurs on the photoreceptor side of Müller cells, especially in the microvillous region. Rabbit retinal Müller cells have a Na+,K+-activated adenosine triphosphatase activity in the same order of magnitude as brain astroglial cells. 相似文献
49.
A controlled study of a specific MAO A reversible inhibitor (R011-1163) and amitriptyline in depressive illness 总被引:2,自引:0,他引:2
A double blind comparative study of amitriptyline and a new reversible MAO A inhibitor R011-1163 was conducted in 25 depressed inpatients over 4 weeks. Response to treatment was assessed with the Hamilton depression rating scale, the Carroll depression self rating scale and the Visual analogue scale. Both drugs produced significant changes in depressive symptomatology (P less than 0.01, MANOVA) and there were no statistically significant differences between drugs (P greater than 0.05 MANOVA). Side effects were of mild to moderate severity with dry mouth the most commonly reported side effect of amitriptyline and vague, generalised headache in patients, treated with R011-1163. 相似文献
50.
Pignatti P Ciapponi L Galle P Hansen MB Massa M Meazza C Paonessa G Novick D Ciliberto G Martini A De Benedetti F 《Clinical and experimental immunology》2003,131(2):355-363
We previously demonstrated that high levels of IL-6/sIL-6R complexes are present in sera of patients with systemic juvenile idiopathic arthritis (s-JIA) and that the amount of IL-6 estimated in the IL-6/sIL-6R complexes is markedly higher than that measured by the B9 assay. Here, we show that two additional bioassays, employing human myeloma XG-1 cells and human hepatoma Hep3B cells, detected serum IL-6 levels similar to those measured by the B9 assay and approximately 10-fold lower than the IL-6 levels estimated to be present in the IL-6/sIL-6R complex. Using an assay for the measurement of the amount of circulating IL-6 complexed with the sIL-6R and available for binding to gp130 (gp130 binding activity), we show that the IL-6/gp130 binding activity is similar to that detected by the bioassays and again significantly lower than that estimated to be present in the IL-6/sIL-6R complex. Addition of recombinant human IL-6 (rhIL-6) to sera of patients or controls results in a markedly lower increase in the gp130 binding activity in patients than in controls. Moreover, sera from s-JIA patients inhibited in a dose dependent manner the gp130 binding activity assay. These results show that sera from patients with s-JIA contain a factor, or factors, that inhibit(s) the binding of the IL-6/sIL-6R complex to gp130. This inhibitory activity does not appear to be due to soluble gp130, C-reactive protein or autoantibodies to IL-6. 相似文献