Lesional elastase activity in psoriasis

Human leukocyte elastase, a neutrophil-derived serine protease, is present in psoriatic lesions in an enzymatically active form. Our purpose was to assess the significance of human leukocyte elastase determinations in estimating the inflammatory activity of psoriatic lesions. A standardized method was used to analyse lesional elastase activity. Elastase activities were correlated with erythema, induration and hyperkeratosis of psoriatic lesions in 54 patients. Lesional elastase activities were also determined during treatment with salt-water bathing and UVB irradiation. Lesional elastase activity correlated with skin induration and was inversely correlated with hyperkeratosis of the lesions. Psoriatic lesions with high elastase activity responded well to therapy, whereas lesions with low elastase activity appeared to be comparatively resistant. This study shows that by quantitative determination of lesional elastase activities it is possible to distinguish predominantly inflammatory from predominantly hyperproliferative psoriasis. The latter shows delayed responsiveness to topical therapy with salt-water bathing plus UVB irradiation.     

酶联免疫法检测PAF受体RNA的聚合酶链反应产物

目的：建立PCR—ELISA法检测附受体RNA。方法：提取20例银屑病病人和20例正常对照组血液单个核细胞标本中的总RNA，用双标记的PAF—R(Biotion与Digoxin标记)及β-actin(Biotin或Fluorescein标记)引物进行RT-PCR，用ELISA检测PCR产物，用传统的琼脂糖凝胶电泳对照。结果；ELISA法和琼脂糖凝胶电泳法对β-actin检测结果均为阳性；ELISA法检测PAF-R，20例病人标本均为阳性，20例对照组16例阳性；琼脂糖凝胶电泳法检测PAF—R，20例病人标本18例阳性，20例对照组16例阳性。结论：PCR-ELISA引物双标记法检测PAF—R操作简单，有较高的敏感性。     

Ras homolog gene family H (RhoH) deficiency induces psoriasis-like chronic dermatitis by promoting TH17 cell polarization

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黑光灯对银屑病血液流变性的影响与疗效

黑光灯对银屑病血液流变性的影响与疗效刘夕茹１李海生２于霖２银屑病是一种常见、易复发的慢性炎症性皮肤病，人们曾对其发病机制以及治疗有过大量探索。本文通过使用黑光灯对其治疗前后的血液流变学指标的检测和综合分析，对该病作进一步探讨。１资料与方法１．１病例选...     

The frequency of type 2 CD8+ T cells is increased in peripheral blood from patients with psoriasis vulgaris

Studies have suggested that psoriasis vulgaris is mediated by type 1 T cells. In this study, we examined both chemokine receptor expression and intracellular cytokine production by circulating T cells to check the type 1/type 2 balance in psoriasis. CCR4⁺ and CXCR3⁺ T cells predominantly produced interleukin-4 and interferon-, respectively. The frequency of interferon--producing cells and that of CXCR3⁺ cells in circulating CD4⁺ T cells were similar for psoriatic patients and healthy control subjects. By contrast, the frequency of CCR4⁺CD8⁺ T cells and CCR4/CXCR3 ratio in circulating CD8⁺ T cells were significantly higher in psoriatic patients than in healthy control subjects. Analysis of intracellular cytokine production also indicated relative increase of type 2 CD8⁺ T (Tc2) cells in peripheral blood from psoriatic patients. The frequency of circulating Tc2 cells directly correlated with Psoriasis Area and Severity Index. Immunohistochemical analysis showed that not only CXCR3⁺CD8⁺ T cells but also a similar number of CCR4⁺CD8⁺ T cells infiltrated the psoriatic epidermis and dermis. Our findings suggest an increase in Tc2 cell number in blood from psoriatic patients, and the association of Tc2 cells with inflammation in psoriasis.     

全身性红斑狼疮、银屑病和胰岛素依赖性糖尿病病人补体成份BfSS亚型的检测

用薄层聚丙烯酰胺凝胶等电聚焦方法对62例全身性红斑狼疮(SLE)、61例寻常型银屑病(PV)及17例胰岛素依赖性糖尿病(IDDM)病人补体第二途径成份B因子SS型的亚型分布及基因频率进行了检测。结果表明,SLB病人BfSS亚基因频率分别为,S_A0.516,S_B0.484;PV为:S_A0.492,S_B0.508,IDDM为:S_A0.676,S_B0.324。与正常人(S_A0.414,S_B0.586)比较,三种疾病的S_A亚基因频率均有不同程度的增高,并伴有S_B亚基因频率的降低。统计结果表明,IDDM与正常人的差异显著(P<0.01),SLE次之(P<0.05),PV不著(P>0.1)。此外,这三种疾病病人BfS_A-S_A亚型的表型频率(分别为30.6%、31.1%和41.1%)都显著高于正常19.3%),统计学表明差异均显著(P<0.05)。     

"银屑灵"治疗银屑病351例临床观察

本文报道用银屑灵治疗银屑病351例,疗程60天,对有效病例延长疗程可提高治愈率.临床痊愈率32.2%,总有效率73.8%,点滴状银屑病疗效最好.随访2年,复发率19.5%.仅少数病例有轻微胃肠道反应,但有6例治疗前发现肝功能异常,作者认为可能属银屑病的肝脏损害,经治疗后转正常.并对治疗机理作了初步探讨.