全文获取类型
收费全文 | 405篇 |
免费 | 112篇 |
国内免费 | 37篇 |
专业分类
耳鼻咽喉 | 8篇 |
儿科学 | 3篇 |
妇产科学 | 9篇 |
基础医学 | 73篇 |
口腔科学 | 8篇 |
临床医学 | 59篇 |
内科学 | 62篇 |
皮肤病学 | 8篇 |
神经病学 | 26篇 |
特种医学 | 10篇 |
外国民族医学 | 1篇 |
外科学 | 24篇 |
综合类 | 71篇 |
预防医学 | 14篇 |
药学 | 76篇 |
中国医学 | 9篇 |
肿瘤学 | 93篇 |
出版年
2024年 | 13篇 |
2023年 | 20篇 |
2022年 | 69篇 |
2021年 | 73篇 |
2020年 | 59篇 |
2019年 | 31篇 |
2018年 | 21篇 |
2017年 | 29篇 |
2016年 | 40篇 |
2015年 | 48篇 |
2014年 | 67篇 |
2013年 | 31篇 |
2012年 | 28篇 |
2011年 | 14篇 |
2010年 | 7篇 |
2009年 | 2篇 |
2008年 | 2篇 |
排序方式: 共有554条查询结果,搜索用时 15 毫秒
71.
Down-regulation of miR-622 in gastric cancer promotes cellular invasion and tumor metastasis by targeting ING1 gene 总被引:1,自引:0,他引:1
Guo XB Jing CQ Li LP Zhang L Shi YL Wang JS Liu JL Li CS 《World journal of gastroenterology : WJG》2011,17(14):1895-1902
AIM:To evaluate the biological and clinical characteristics of miR-622 in gastric cancer. METHODS:We analyzed the expression of miR-622 in 57 pair matched gastric neoplastic and adjacent non-neoplastic tissues by quantitative real-time polymerase chain reaction. Functional analysis of miR-622 expression was assessed in vitro in gastric cancer cell lines with miR-622 precursor and inhibitor. The roles of miR-622 in tumorigenesis and tumor metastasis were analyzed using a stable miR-622 expression plasmid in ... 相似文献
72.
目的 探究急性冠脉综合征患者循环miR-208b-3p表达水平与心室重构的关系。方法 收集140例西安市第九医院心血管内科2015年11月至2016年12月确诊为急性冠脉综合征患者,实时定量PCR(quantification PCR,qPCR)检测样本miR-208b-3p的表达水平,根据miR-208b-3p表达水平高低以每组同样患者数分为四组,分别比较四组住院及出院1年随访超声测量结果,并对测量值变化率做进一步对比分析。结果 住院期间各超声测量组患者例数之间差异无统计学意义(p>0.05),出院1年各左心室舒张末期内径(left ventricular end-diastolic dimension,LVDd)组和各左心室射血分数(left ventricular ejection fraction,LVEF)组患者例数的差异具有统计学意义(p=0.046;p=0.036),各左心室短轴缩短率(fraction shortening,FS)组差异无统计学意义(p>0.05)。变化率分析显示各组LVDd变化率分别为(-0.410±0.125、0.024±0.156、0.082±0.152、0.004±0.078)(p=0.326);各组FS变化率分别为(0.081±0.379、0.074±0.209、0.061±0.258、0.123±0.310)(p=0.896);各组LVEF变化率分别为(0.082±0.035、0.046±0.035、0.022±0.037、-0.082±0.052)(p=0.034)。对各组LVEF测量值变化率进行两两对比显示高危组分别与低危组及中低危组差异具有统计学意义(p=0.010;p=0.042)。结论 循环miR-208b-3p与远期心室重构相关,并且对LVEF值的影响最大。 相似文献
73.
目的 本研究旨在探讨miR-18B-5p在肝癌中的作用及其机制。
方法 检测miR-18B-5p在肝癌患者血清中的表达水平,对其临床意义进行分析。下调miR-18B-5p表达水平,检测miR-18B-5p在肝癌细胞系增殖、迁移和侵袭中的作用,用实时荧光定量聚合酶链反应和荧光素酶报告法探讨其作用机制。
结果 低miR-18B-5p表达组患者生存率高于高miR-18B-5p表达组(P<0.05),多因素分析结果显示,miR-18B-5p的表达水平(RR:2.064,95%CI:1.522~2.800)和年龄(RR:1.762,95%CI:1.347~2.305)是患者生存的影响因素,HepG2细胞表达的miR-18B-5p水平高于Bel-7402组、MHCC97组和Hep3B组(P<0.05),Si-miR-18B-5p细胞系的Si-miR-18B-5p表达和细胞增殖低于HepG2细胞系(P<0.05),在Si-miR-18B-5p细胞系miR-18B-5p下调,细胞迁移和侵袭能力低于HepG2细胞系(P<0.05),Si-miR-18B-5p细胞中BTG3的mRNA表达高于HepG2细胞系(P<0.05),BTG3 WT相对荧光素活性低于BTG3 Mut(P<0.05)。
结论 MiR-18B-5p可作为肝癌新的治疗靶点和预后标志物。 相似文献
74.
目的 研究MiR-138通过HTERT作为下游靶基因,对人乳腺癌MCF-7细胞端粒酶活性的调控作用及端粒稳定性的影响。方法 在人乳腺癌MCF-7细胞中瞬时转染MiR-138 模拟物,用MTT法检测细胞增殖活性,并于转染后48h,用实时定量RT-PCR检测端粒酶催化亚单位HTERT表达、TRAP Assay检测端粒酶活性,同时对细胞进行53BP1 抗体免疫荧光染色及端粒的FISH染色。结果 转染后48h,MiR-138模拟物处理的MCF-7细胞HTERT表达水平比对照细胞降低2.18倍(2-△△Ct),端粒酶活性比对照细胞降低2.69倍,53BP1聚集形成的凝集点(Foci),部分与端粒位点重合,比率达到20.62%±1.55% 。结论 MiR-138以HTERT作为下游靶分子,调控MCF-7细胞端粒酶活性,影响细胞端粒稳定性。 相似文献
75.
Jiayu Li Xuefei Li Shengxiang Ren Xiaoxia Chen Yishi Zhang Fei Zhou Mingchuan Zhao Chao Zhao Xiu Chen Ningning Cheng Yinmin Zhao Caicun Zhou Fred R. Hirsch 《Oncotarget》2014,5(17):7902-7916
Several randomized trials have demonstrated non-small cell lung cancer (NSCLC) patients with activating epidermal growth factor receptor (EGFR) mutations can achieve favorable clinical outcomes on treatment with EGFR tyrosine kinase inhibitors (TKIs). EGFR mutation is considered as a predictive marker for efficacy of EGFR-TKIs in NSCLC. Here we show miR-200c overexpression was correlated with the epithelial phenotype and sensitivity to gefitinib in EGFR wild-type NSCLC cell lines. Up-regulated miR-200c could regain the sensitivity to gefitinib in the EGFR wild-type cell lines and miR-200c could regulate epithelial to mesenchymal transition through PI3K/AKT and MEK/ERK pathways. NSCLC patients at advanced stage (N=150) who received EGFR-TKIs (gefitinib or erlotinib) as second- or third-line therapy from September 2008 to December 2012 were included in the study. In 66 NSCLC patients with wild-type EGFR, high levels of miR-200c expression was associated with higher disease control rate (DCR), longer progression-free survival (PFS) and longer overall survival (OS) compared with low miR-200c expression subgroup. In the subgroup with EGFR mutation, the trend remained the same but not statistically significant. Overall, these findings indicated that miR-200c might be a predictive biomarker for sensitivity to EGFR-TKIs in advanced NSCLC patients with wild-type EGFR. 相似文献
76.
Introduction
The well-known oncomiR-miR-21 was previously reported oncogenic activity in lung cancer. We sought to determine the expression of all predicted target genes of miR-21 and their potential function, pathways and networks, which are involved in the biological behavior of lung cancer.Methods
After a systematic review of English language studies of lung cancer-related molecules were pooled; genes were classified in three functional groups by gene ontology (GO) analysis. The key molecules were indentified by establishing lung cancer related networks and pathways. MiR-21 targets were predicted by computational method, followed by screening for matched gene symbols in NCBI human sequences and GO, pathway and network analysis. MiR-21 targets and their network, which are involved in the malignant mechanisms of lung cancer, were obtained by the final integrative analysis.Result
We indentified the potential functions, pathways and networks of lung cancer relating molecules and miR-21 targets respectively in the initial analysis. In the final integrative analysis of lung cancer related miR-21-targets analysis, 24 hub genes were identified by overlap calculation, suggesting that miR-21 may play an important role in the development and progression of lung cancer through JAK/STAT signal pathway, MAPK signaling pathway, Wnt signaling pathway, cell cycle, PPAR signaling pathway, apoptosis pathway and other pathways.Conclusion
Our data may help researchers to predict the molecular mechanisms of miR-21 in the development and progression of lung cancer comprehensively. Moreover, the present data indicate that miR-21-targets may be a series of promising candidates as biomarkers for lung cancer. 相似文献77.
Psoriasis is a multifactorial, recurring, and chronic inflammatory skin disease characterized by hyperproliferation of keratinocytes. Evidence is rapidly accumulating for the role of microRNAs in psoriasis. The object of the study was to explore the functions and precise mechanism of miR-142–3p in human keratinocyte HaCaT cells in the presence of M5. Here, the results showed that miR-142–3p expression was heightened in HaCaT cells induced by M5. In addition, inhibition of miR-142–3p dramatically restricted cell proliferation and enhanced apoptosis in HaCaT cells exposed to M5, as exemplified by a decrease in the antiapoptotic Bcl-2 protein, concomitant with an increase in the proapoptotic proteins Bax. Moreover, depleting miR-142–3p effectively ameliorated M5-induced inflammation response, as reflected by the attenuation of multiple inflammatory factors. Importantly, Sema3A was identified as an authentic target of miR-142–3p, and indeed regulated by miR-142–3p. Mechanistically, silencing of Sema3A effectively abolished the anti-proliferative, apoptosis-promoting, and anti-inflammatory effects of miR-142–3p inhibition in keratinocytes. Taken together, these data elucidated that repression of miR-142–3p protect HaCaT cells against M5-induced hyper-proliferation and inflammatory injury by suppressing its target Sema3A, implying that the miR-142–3p/Sema3A axis may be a new target for preventing keratinocyte injury process. These findings provide a new and better understanding of the mediating role of miR-142–3p in psoriasis. 相似文献
78.
BackgroundUncontrol cell growth and proliferation is acknowledged to responsible for cancer-related deaths by disorganizing the balance of growth promotion and growth limitation. Aberrant expression of microRNA play essential roles in cancer development, leads to cell proliferation, growth and survival, and promotes the development of various human tumors, including osteosarcoma. Elucidating the molecular mechanism of this abnormality in osteosarcoma carcinogenesis may improve diagnostic and therapeutic strategies for this malignancy.MethodsThe expression of miR-664 in osteosarcoma cell lines and osteosarcoma tissues was examined using real-time PCR. The effects of miR-664 on osteosarcoma cell proliferation were evaluated by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, colony formation and Anchorage-independent growth ability assay. The effect of miR-664 on FOXO4 was determine by luciferase assays and western blot assay.ResultsThe expression of miR-664 was markedly upregulated in osteosarcoma cell lines and tissues, and upregulation of miR-664 enhanced, whereas downregulation of miR-664 inhibited the proliferation of osteosarcoma cells in vivo. Furthermore, using bioinformatics and biological approaches, we showed that miR-664 directly targeted and suppressed the expression of tumor suppressors FOXO4.ConclusionsOur findings suggest that miR-664 functions as an oncogene miRNA and has an important role in promoting human osteosarcoma cell proliferation by suppressing FOXO4 expression. These data suggests that miR-664 may represent a novel therapeutic target of microRNA-mediated suppression of cell proliferation in osteosarcoma. 相似文献
79.
AimLncRNA MALAT1 is involved in regulation of angiogenesis, however, its expression and mechanism in infantile hemangioma (IH) are less reported. The study aimed to investigate MALAT1 in IH and to reveal the potential mechanism of MALAT1 acting on IH.MethodsIsolated form IH tissue, human CD31+ hemangioma endothelial cells (HemECs) were cultured and sorted by magnetic-activated cell sorting (MACS). Quantitative real-time (qRT)-PCR was performed to detect the expressions of MALAT1, miR-206 and VEGFA. The correlations among MALAT1, miR-206 and VEGFA were confirmed by bioinformatics analysis and dual-luciferase reporter assay. The effects of MALAT1, miR-206 and VEGFA on cell proliferation were detected by cell counting kit-8 (CCK-8) and cell colony formation assay. Flow cytometry, wound scratch, Transwell and Tube formation assay were performed to determine cell apoptosis, migration, invasion and vasoformation, respectively. Apoptosis-related proteins were determined by Western blot.ResultsThe results showed that MALAT1 and VEGFA were high-expressed and miR-206 was low-expressed in IH tissues. SiMALAT1 negatively affected the cell proliferation, migration, invasion and vasoformation of HemECs and promoted apoptosis of HemECs. Moreover, Bcl-2 expression was significantly inhibited and the expressions of Bax and c cleaved-3 were greatly promoted. MALAT1 directly targeted and inhibited the expression of miR-206, and VEGFA was predicted to be the target gene for miR-206. SiMALAT1 suppressed the cell proliferation, migration, invasion and vasoformation of HemECs through modulating miR-206/VEGFA axis.ConclusionKnock-down of MALAT1 inhibits the growth of HemECs through regulating miR-206/VEGFA axis, indicating that MALAT1 is a potential therapeutic mechanism for the treatment of IH. 相似文献
80.
Retinoblastoma (RB) is the most frequent primary intraocular cancer. It has been demonstrated by previous studies that retinoblastoma is initiated primarily by the inactivation of the retinoblastoma Rb1 gene in retinal cells. However, additional genetic alterations than Rb1 mutation could play important roles in the process of transforming benign retinal cells into retinoblastoma tumor cells. In this study, we identified that microRNA miR-433 is one of such genetic factors. We found that the expression levels of miR-433 were downregulated in RB tissues. We also determined that miR-433 negatively regulated RB cell proliferation, migration and invasion, and induced cell cycle arrest and apoptosis of RB cells. We used bioinformatics method to predict and confirmed that Notch1 and PAX6 were miR-433 target genes in RB cells. Importantly, we demonstrated that restoration of Notch1 and PAX6 expression partially rescued the inhibition of cell proliferation and metastasis induced by miR-433 overexpression, suggesting that miR-433 regulates RB cell proliferation and metastasis through suppressing the expression of Notch1 and PAX6. 相似文献