骨髓干细胞移植治疗缺血性心脏病的研究进展

干细胞具有自我更新，多向分化能力，在适宜的体外培养中干细胞能够稳定生存增殖并保持多向分化潜能，是便于体外操作的理想的靶细胞。本综合近几年国内外献，阐述了骨髓干细胞通过移植技术移植至试验动物的病损心脏，可以分化增殖为心肌细胞和血管结构，从而改善缺血的心脏功能。因此具有广阔的临床应用前景，值得进一步研究。     

重组人乳头瘤病毒11型L1蛋白在昆虫细胞sf 9中的表达条件优化研究

目的 确定利用昆虫细胞一杆状病毒表达系统表达重组人乳头瘤病毒11型L1蛋白(HPV11 L1)的最适表达条件.方法 通过间接免疫荧光法鉴定目的蛋白表达情况;用蛋白印迹法(Western blot)确定在不同感染复数(MOI)、不同表达时间条件下目的蛋白的最适表达条件.结果 当sf9细胞密度为2.0×106/ml时,以MOI值为2.0的重组杆状病毒接种,悬浮表达96 h收获为最适表达条件.结论 确定了目的蛋白的最适表达条件,为进一步大量表达及纯化提供参考.     

PGK1-coupled HSP90 stabilizes GSK3β expression to regulate the stemness of breast cancer stem cells

Objective: Glycogen synthase kinase-3β(GSK3β) has been recognized as a suppressor of Wnt/β-catenin signaling, which is critical for the stemness maintenance of breast cancer stem cells. However, the regulatory mechanisms of GSK3β protein expression remain elusive.Methods: Co-immunoprecipitation and mass spectral assays were performed to identify molecules binding to GSK3β, and to characterize the interactions of GSK3β, heat shock protein 90(Hsp90), and co-chaperones. The role of PGK1 in Hsp90 ch...     

Safety and efficacy of anti-EGFR monoclonal antibody (SCT200) as second-line therapy in advanced esophageal squamous cell carcinoma

Objective: The mainstay treatment of esophageal squamous cell carcinoma(ESCC) involves chemotherapy and immunotherapy. However, alternative therapies are required for patients who are refractory or intolerant to existing therapies.Methods: In this single-arm, multicenter, open-label phase Ib study, 30 patients received an intravenous infusion of SCT200, an antiepidermal growth factor receptor(EGFR) monoclonal antibody, 6.0 mg/kg once a week for 6 weeks, followed by 8.0 mg/kg once every 2 weeks u...     

Eliciting pyroptosis to fuel cancer immunotherapy: mechanisms and strategies

Immune checkpoint blockade(ICB) therapy has recently shown promise in treating several malignancies. However, only a limited number of patients respond to this treatment, partially because of the “immune cold” condition of the tumor immune microenvironment. Pyroptosis is a type of gasdermin-mediated programmed cell death that often leads to inflammation and immune responses. Many studies on the mechanism and function of pyroptosis have led to increasing recognition of the role of pyroptosis in m...     

Exosomes with FOXP3 from gene-modified dendritic cells ameliorate the development of EAE by regulating the balance of Th/Treg

Objective: To investigate the efficiency and potential mechanisms of exosomes from dendritic cells (DCs) transfected with Forkhead box protein P3 (FOXP3) in the development of experimental autoimmune encephalomyelitis (EAE).Method: Mouse bone marrow-derived immature DCs were loaded with adenovirus carrying FOXP3 gene, and exosomes were generated. Then the exosomes with FOXP3 (FOXP3-EXOs) were co-cultured with CD4+T cell in vitro to evaluate their potential on CD4+T cell proliferation and differentiation, and injected into EAE mice to assess their effects on the development of EAE.Result: FOXP3-EXOs were effective to inhibit the CD4⁺T cell proliferation and the production of Interferon gamma (IFN-γ), interleukin (IL)-6, and IL-17, while they promoted the production of IL-10 in vitro. Moreover, FOXP3-EXOs treatment significantly decreased the neurological scores, reduced the infiltration of inflammatory cells into the spinal cord, and decreased demyelination in comparison to saline and Con-EXOs treated EAE mice. Moreover, the FOXP3-EXOs treatment resulted in obvious increases in the levels of regulatory T (Treg) cells and IL-10, whereas levels of T helper 1 (Th1) cells, Th17 cells, IFN-γ, IL-6, and IL-17 decreased significantly in the splenocyte culture of EAE mice.Conclusion: The present study preliminarily investigated the effects and potential mechanisms of FOXP3-EXOs in EAE and revealed that the FOXP3-EXOs could inhibit the production of Th1 and Th17 cells and promote the production of Treg cells as well as ameliorate the development of EAE. The neuroprotective effects of FOXP3-EXOs on EAE are likely due to the regulation of Th/Treg balance.     

60Co γ照射加深低温保存对大鼠同种带瓣主肺动脉免疫原性的作用

目的 本实验应用60Co γ射线照射加液氮深低温保存大鼠同种带瓣主肺动脉(valvedpulmonary homograft conduits,VPHC),并建立大鼠VPHC颈总动脉移植模型,观察不同照射剂量对同种带瓣主肺动脉免疫原性的作用,寻找合适照射剂量,为进一步基础研究及临床应用提供理论依据.方法 1月龄封闭群Wistar大鼠50只作为供体鼠,雌雄不拘,随机分为单纯深低温保存组(实验对照组)、300 Gy照射组、600 Gy照射组、900 Gy照射组和1200 Gy照射组;成年雄性SD大鼠50只作为受体鼠,随机分为5组;另取10只SD大鼠行颈总动脉切断吻合术,作为空白对照组.应用流式细胞术对VPHC移植术后1周、2周、3周受体鼠外周血行CD4+T细胞及CD8+T细胞检测.结果 照射组VPHC移植术后1周、2周、3周CD4+T细胞、CD8+T细胞百分率及CD4+/CD8+与空白对照组相比差异有统计学意义(P<0.05),与实验对照组相比差异有统计学意义(P<0.05).结论 应用60Co γ对VPHC进行照射在保持VPHC活性的同时明显降低了其免疫原性.     

间充质干细胞的免疫调节作用

间充质干细胞(MSC)已成功从动物体尤其骨髓中分离获得.干细胞,具有向多种组织和细胞如骨、脂肪、成软骨细胞等分化的潜能,这种潜能越来越引起人们关注,它可向受损的组织定向迁移以修复受损组织.体外资料研究表明,不管协同刺激信号存在与否,都具有低免疫原性.在体外MSC还具有免疫抑制活性,可抑制同种异体抗原或丝裂霉素刺激的T细胞扩增,阻止细胞毒性T细胞增殖,同时还具有调节炎性细胞的免疫活性使其向抗炎细胞分化的作用.因此,系统了解.MSC的生物学、免疫学特性将为人们深入阐明其免疫调节机制,并将其用于治疗自身免疫性病、组织修复及移植提供依据.     

Optic Disc Cupping in Arteritic Anterior Ischemic Optic Neuropathy Resembles Glaucomatous Cupping

Five cases of anterior ischemic optic neuropathy secondary to biopsy-proven giant cell arteritis are presented. In each case, cupping of the optic disc, which closely resembled glaucomatous cupping, was observed in the affected eye. The presence of glaucoma was ruled out on the basis of normal intraocular pressures and normal tonographic measurements of facility of outflow. These cases indicate that arteritic ischemic optic neuropathy can result in optic disc cupping, which closely resembles glaucomatous cupping. The similarities in the appearance of cupping of these discs with that seen in eyes with glaucoma suggest that the pathogenesis of cupping in glaucoma and in arteritic ischemic optic neuropathy may share some common mechanisms.     

针灸治疗溃疡性结肠炎的临床观察

目的 观察针灸治疗溃疡性结肠炎的疗效。方法与结果 采用温针灸：下合穴,募穴为主,配合辨证取穴,内服中成药,治疗溃疡性结肠炎62例,总有效率91．94％,T细胞亚群治疗后也接近正常。结论 显示本法有促进人体免疫系统内环境趋于平衡的作用。