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71.
树突状细胞负载肝癌抗原肽疫苗体外诱导特异性免疫学反应的研究 总被引:1,自引:1,他引:1
目的:研究树突状细胞(dendritic cell,DC)负载肝癌抗原肽EPVTKAEML体外诱导特异性CTL的能力及其抑癌效果。方法:用顺序特异引物聚合酶链反应技术(PCR—SSP)选择HLA—B7表型供者,从脾组织中分离、培养DC-EPVTKAEML特异性CTL。用^51Cr释放法检测CTL的杀伤活性,并用抗HLA-1分子单抗(mAb)进行杀伤抑制实验。结果:找到4例HLA-B7杂合子供者,用DC负载HLA-B7限制的抗原肽EPVTKAEML可诱导特异性CTL反应,对肝癌细胞HHCC有较强的杀伤作用。结论:DC负载抗原肽EPVTKAEML在体外可诱发较强的特异性免疫反应。 相似文献
72.
Peptide binding proteins of the major histocompatibility complex consist of the “classical” class Ia and “nonclassical” class
Ib genes. The gene organization and structure/function relationship of the various exons comprising class I proteins are very
similar among the class Ia and class Ib genes. Although the tissue-specific patterns of expression of these two gene families
are overlapping, many class Ib genes are distinguished by relative low abundance and/or limited tissue distribution. Further,
many of the class Ib genes serve specialized roles in immune responses. Given that the coding sequences of the class Ia and
class Ib genes are highly homologous we sought to examine the promoter regions of the various class Ib genes by comparison
to the well characterized promoter elements regulating expression of the class Ia genes. This analysis revealed a surprising
complexity of promoter structures among all class I genes and few instances of conservation of class Ia promoter regulatory
elements among the class Ib genes. 相似文献
73.
Arnaiz-Villena A Karin M Bendikuze N Gomez-Casado E Moscoso J Silvera C Oguz FS Sarper Diler A De Pacho A Allende L Guillen J Martinez Laso J 《Tissue antigens》2001,57(4):308-317
Turkish and Kurdish HLA profiles are studied for the first time. The comparative study of their allele frequencies, characteristic haplotypes, genetic distances with other Mediterraneans is complemented by neighbor-joining dendrograms and correspondence analyses. Turks, Kurds, Armenians, Iranians, Jews, Lebanese and other (Eastern and Western) Mediterranean groups seem to share a common ancestry: the older "Mediterranean" substratum. No sign of the postulated Indo-European (Aryan) invasion (1200 B.C.) is detected by our genetic analysis. It is concluded that this invasion, if occurred, had a relatively few invaders in comparison to the already settled populations, i.e. Anatolian Hittite and Hurrian groups (older than 2000 B.C.). These may have given rise to present-day Kurdish, Armenian and Turkish populations. 相似文献
74.
Yusuf Orhan Adil Azezli Mahmut Çarin Ferihan Aral Ergin Sencer Senay Molvalılar 《Journal of clinical immunology》1993,13(5):339-343
To evaluate the association of HLA types with Turkish patients with Graves' disease, HLA typing, clinical findings, and thyroid antibodies were correlated. The HLA types, clinical findings (ophthalmopathy and age at onset), and thyroid stimulating hormone (TSH) receptor (TRAb) and antithyroid microsomal antibodies (MAb) were analyzed. Seventy Turkish patients with Graves' disease and 306 control subjects were assessed. Serological HLA typing was performed in HLA A, B, C, DR, and DQ loci. There was a significantly increased prevalence of HLA B8, B49, DR3, DR4, and DR10 in Graves' disease. The association of Graves' disease with HLA DR3 was found to be less strong than previously described. The HLA DR4 antigen may contribute to the predisposition of Graves' disease in Turkey. The results suggest that HLA B7, B13, DR7, DQw2, and DQw3 may confer a protective effect for Graves' disease in Turkey. Patients carrying HLA B12, B18, and B44 haplotypes had a tendency to develop the disease at a later age. The difference from the other studies may be the result of the selection of the controls; in part, of the variability in serological typing reagents; and, also, of the rather weak HLA associations with the disease.This study was presented in part at the Annual Meeting of the National Endocrinology and Diabetes Association, Bursa, Turkey, May 25–28, 1992. 相似文献
75.
Takeshi Tana Nobuhiro Kamikawaji Christopher J. Savoie Tohru Sudo Yurika Kinoshita T. Sasazuki 《Journal of human genetics》1998,43(1):14-21
Susceptibility to a series of autoimmune diseases is strongly associated with particular HLA class II alleles. Identification
of T cell clones and antigenic epitopes bound by HLA class II molecules involved in autoimmune diseases is critical to understanding
the etiology of these HLA class II-associated diseases. However, establishment of T cell clones in autoimmune diseases is
difficult because the antigenic peptides are unknown. Peptide library methods which include all possible peptide sequences
offer a potentially powerful tool for the detection of cross-reactive antigenic peptides recognized by T cells. Here, we reduced
the number of peptides per mixture by utilizing the known binding motifs of peptides for the HLA-DRB1*0405 molecule and evaluated
the effectiveness of this library design. Each library mixture evoked a strong proliferative response in the unprimed peripheral
blood lymphocytes (PBL) from HLA-DRB1*0405-positive donors but little or no response in the PBL from HLA-DRB1*0405-negative donors. The library also detected antigenic peptides that activated three antigen-specific T cell lines restricted
by HLA-DRB1*0405, with different specificities. The motif-based approach thus presents a powerful method for monitoring T
cells in large, heterogeneous T cell populations and is useful for the identification of the mimic peptide epitopes of T cell
lines and clones.
Received: October 3, 1997 / Accepted: October 23, 1997 相似文献
76.
Mayor NP Cox ST McWhinnie AJ Argüello JR Shaw BE Little AM Madrigal JA Marsh SG 《Tissue antigens》2005,65(1):107-109
We report here the full-length sequence of a novel HLA-A*0301 allele, A*03010103, which differs from A*03010101 by a single nucleotide substitution (G>T) at position 492 within intron 2. The variant was originally identified by Reference Strand-mediated Conformational Analysis (RSCA) and was confirmed by cloning and sequencing. The difference in RSCA mobility between A*03010101 and A*03010103 demonstrates the sensitivity of RSCA to detect single nucleotide polymorphisms. 相似文献
77.
Influence of age and HLA type on interferon-gamma (IFN-gamma) responses to a naturally occurring polymorphic epitope of Plasmodium falciparum liver stage antigen-1 (LSA-1) 下载免费PDF全文
Bucci K Kastens W Hollingdale MR Shankar A Alpers MP King CL Kazura JW 《Clinical and experimental immunology》2000,122(1):94-100
Antigenic polymorphism and HLA restriction may limit the immunogenicity of a subunit vaccine against liver-stage Plasmodium falciparum. We examined 59 clinical isolates and five laboratory clones of P. falciparum for polymorphism in the N- and C-terminal regions of LSA-1, evaluated binding of the corresponding peptides to selected HLA class I alleles, and measured IFN-gamma responses in residents of a malaria-endemic area of Papua New Guinea where HLA-A*1101, -24, -B13, and -B40 are the most common class I alleles. LSA-1 polymorphism was limited to a single non-synonymous mutation encoding serine (S), proline (P), or threonine (T) at amino acid 85. Nine-mer 84-92 peptides with S, T, or P at the primary anchor position bound differentially to HLA-A11, -A2, and -B7. IFN-gamma ELISPOT responses increased with age in malaria-exposed subjects: 14-16% and 30-36% of 2-5- and 6-54-year-olds, respectively, had > or =10 IFN-gamma-secreting cells/106 peripheral blood mononuclear cells when stimulated with at least one peptide variant (P<0.05). IFN-gamma responses to all three peptides were also greater for older than younger individuals. No children < 3 years old had lymphocytes that responded to all three 84-92 peptides, whereas 45% of adults (mean age 48 years) had aggregated IFN-gamma responses. These data support the notion that age-related cumulative exposure to P. falciparum increases the frequency of IFN-gamma responses to polymorphic epitopes of liver-stage antigens such as LSA-1. 相似文献
78.
79.
E.G. De la Concha M. Fernandez-Arquero J.L. Mendoza L. Conejero M.A. Figueredo J. Perez de la Sema M. Diaz-Rubio A. Ruiz de Leon 《Tissue antigens》1998,52(4):381-384
Abstract: Achalasia is a motor disorder of the esophagus resulting in functional obstruction. The cause of the lesion is unknown although genetic and immunologic factors have been suggested. An association with serological HLA epitopes has been previously reported. In this study, we have further examined this HLA class II association with susceptibility to achalasia by DNA based methods. Achalasia patients ( n =40) and healthy controls ( n = 275), all Caucasians and unrelated, were included in the analysis. The strongest associations were with HLA-DQAl*0101 and two HLA-DQαβ heterodimers having their a chain encoded by this allele. Moreover, relative risk was significantly higher in DQAl*0101 homozygotes as compared to heterozygotes and results suggested that DQB1*02 may have a protective role. 相似文献
80.
目的 探讨可溶性 HLA-G1(sHLA-G1)对人 NK-92 细胞杀伤活性的抑制与细胞表面免疫球蛋白样转录分子 2(ILT2)和杀伤细胞免疫球蛋白样受体 2DL4(KIR2DL4)受体的关系。 方法 ①通过原核表达技术获得 sHLA-G1 重组蛋白(重组蛋白),并采用蛋白质印迹法进行鉴定。②取 NK-92 细胞,加入终浓度 20 μg/ml 的重组蛋白分别培养 10、30 min,再分别加入抗 HLA-G1/G5、抗ILT2 和抗 KIR2DL4 抗体,采用流式细胞术检测各组 NK-92 细胞表面 sHLA-G1 和 ILT2、KIR2DL4 受体表达阳性率;以 NK-92 细胞单独培养作为对照组。③以人白血病 K562 细胞为靶细胞,以经不同方式处理的 NK-92 细胞为效应细胞,效靶比为5:1,共同培养 2 h,采用流式细胞术检测 NK-92 细胞对 K562 细胞的杀伤率。NK-92 细胞处理方式为单纯重组蛋白处理(分别加入终浓度为 0、10、20 μg/ml 的重组蛋白培养 30 min)和表面受体封闭 + 重组蛋白处理(分别加入抗 ILT2、抗 KIR2DL4、抗 LT2 + 抗 KIR2DL4 抗体培养 30 min,再分别加入终浓度为 0、10、20 μg/ml 的重组蛋白培养 30 min)。 结果 ①蛋白质印迹分析表明所获重组蛋白为带有组氨酸标签的特异蛋白。②NK-92 细胞与 20 μg/ml 重组蛋白共培养 30 min 后,sHLA-G1 表达阳性率明显高于而 ILT2、KIR2DL4 受体表达阳性率均明显低于对照组(均 P < 0.05)。③以终浓度 0、10、20 μg/ml 的重组蛋白处理的 NK-92 细胞对 K562 细胞的杀伤率分别为 39.79% ± 2.00%、27.79% ± 0.75%、21.36% ± 0.67%(两两比较,均 P < 0.01);单独封闭 ILT2 受体,杀伤率分别为 23.09% ± 1.63%、21.13% ± 0.38%、18.42% ± 0.47%(两两比较,均 P < 0.01);单独封闭 KIR2DL4 受体,杀伤率分别为 30.74% ± 0.44%、26.03% ± 0.38%、21.15% ± 0.35%(两两比较,均 P < 0.01)。 结论 sHLA-G1 通过与 NK-92 细胞表面 ILT2 和 KIR2DL4 受体直接结合而抑制 NK-92 细胞的杀伤活性。 相似文献