Superoxide dismutase-like immunoreactivity in spheroids in Hallervorden-Spatz disease

Iron accumulation in the basal ganglia and spheroid formation are pathological hallmarks of Hallervorden-Spatz disease (HS). Since an overaccumulation of iron (iron thesaurosis) that exceeds the binding capacity of ferritin could cause oxidative damage, we studied the possible role of oxidative stress in the pathogenesis of HS. The basal ganglia and spinal cord from patients with HS were investigated at autopsy, using histochemistry for iron and immunohistochemistry for Cu/Zn superoxide dismutase (SOD1), Mn superoxide dismutase (SOD2) and ferritin. SOD1-like immunoreactivity (IR), SOD2-IR and ferritin-IR occurred frequently in spheroids observed in the basal ganglia, and associated iron accumulation indicated the possible existence of increased oxidative stress in HS patients. Spheroids in the spinal cord showed intense SOD1-IR and SOD2-IR in HS, in sharp contrast with the occasional weak SOD1-IR and SOD2-IR observed in spheroids from patients with amyotrophic lateral sclerosis (ALS). Neither increased ferritin-IR nor iron accumulation were observed in spinal spheroids from HS and ALS patients. These data may suggest that, at least in the spinal cord, SOD1-IR and SOD2-IR in spheroids in HS patients do not result from oxidative stress directly related to iron accumulation. Received: 15 March 1996 / Revised accepted: 15 July 1996     

A case of acute infectious mononucleosis presenting with very high ferritin

Hepatitis is an important but uncommon manifestation of acute Epstein Barr infection. Infectious mononucleosis is usually a disease of young adults. We report a case of infectious mononucleosis in a 72-year old jaundiced gentleman with ferritin level of 2438 that normalised on clinical improvement.     

肿瘤相关糖类抗原CA125、CA72-4和铁蛋白联合检测对卵巢癌的诊断价值

目的评价外周血清肿瘤相关糖类抗原CA125、CA72—4和铁蛋白（Ferritin）联合测定对卵巢癌的诊断价值。方法采用电化学发光免疫分析法测定122例卵巢癌、60例良性卵巢肿瘤和50例健康体检者外周血清的CA125、CA72—4和铁蛋白（Ferritin）的水平,采用t检验和χ^2检验进行数据统计分析。结果卵巢癌组CA125、CA72—4和Ferritin的水平及阳性率分别为（436．4±137．5）U／ml与72．9％、（43．2±31．5）U／ml与47．5％、（1213．5±782．6）ng／ml与53．3％,明显高于良性卵巢肿瘤组的（62．3±25．6）U／ml与18．3％、（10．4±4．8）U／ml与15．0％、（623．4±214．6）ng／ml与32．0％及健康对照组的42．5U／ml与2．0％、（8．8±3．1）U／ml与4．0％、（544．6±135．4）ng／ml与16．0％,P〈0．01。随着临床分期的逐步升高,CA125、CA72—4、Ferritin阳性率和水平也不同程度升高。结论外周血清CA125、CA72—4和Ferritin联合检测可提高卵巢癌的检出率,对卵巢良性肿瘤和卵巢癌的诊断及鉴别诊断具有重要临床意义,弥补了单独检测CA125的不足。     

Distribution of ferritin in the rat hippocampus after kainate-induced neuronal injury

A gradual increase in iron occurs in the lesioned hippocampus after neuronal injury induced by the excitotoxin kainate, and the present study was carried out to investigate whether this increase in iron might be associated with changes in expression of the iron binding protein, ferritin. An increase in ferritin immunoreactivity was observed in glial cells of the hippocampus, as early as three days after intracerebroventricular injections of kainate. The number of ferritin positive cells peaked four weeks after the kainate injection, and decreased eight and twelve weeks after injection. They were found to be mostly microglia and oligodendrocytes by double immunofluorescence labeling with glial markers. A number of ferritin-labeled endothelial cells were also observed via electron microscopy. The decline in ferritin immunoreactivity four weeks after the injection of kainate is accompanied by an increase in the number of ferric and ferrous iron positive cells in the lesioned tissue. A substantial non-overlap between ferritin and iron-containing cells was observed. In particular, spherical ferric or ferrous iron-laden cells in the degenerating hippocampus were unlabeled for ferritin for long time periods after the kainate injection. An increase in iron, together with a reduced expression of iron binding proteins such as ferritin at long time intervals after kainate lesions, could result in a relative decrease in ferritin-induced ferroxidase activity and the presence of some of the iron in the ferrous form. It is postulated that this may contribute to chronic neuronal injury, following acute kainate-induced neurodegeneration.     

铁蛋白鉴别胸腹液良恶性质的临界值选择

探讨铁蛋白ferrztin,Ft在鉴别胸腹液良、恶性质的临界值。用RIA法测定218份由不同病因引起的胸腹液标本铁蛋白(pleuraleffusionferritin,PFt)及其同期血清铁蛋白(SFt)。依据临床确诊资料,将标本进行良、恶性病例分组,比较两组PFt、SFt,计算PFt/SFt比值,用ROC曲线选择PFt用于良、恶性质鉴别的最佳临界值。结果表明,良性组PFt为142.4±38.6μg/L,SFt为89.7±43.5μg/L,PFt/SFt为1.46±0.55;恶性组PFt为576.5±239.1μg/L,SFt为189.6±81.7μg/L,PFt/SFt为3.67±1.48;PFt用于鉴别胸腹液良、恶性质的临界值为400μg/L。以恶性积液PFt≥400μg/L、PFt/SFt≥3,良性积液PFt<400μg/L、PFt/SFt<3为实验诊断标准鉴别胸腹腔积液的良、恶性质,灵敏度为84.5%、特异性为87.5%,准确性为92.8%。     

血清铁蛋白与儿童注意缺陷多动障碍关系的Meta分析

目的：探讨血清铁蛋白与儿童注意缺陷多动障碍(ADHD)发生的关系。方法：利用计算机在万方数据库、中国全文期刊数据库、维普数据库和PubMed数据库中检索血清铁蛋白与儿童ADHD发生的相关文献,并应用RevMan 5.0软件对符合入选标准的文献进行异质性检验和效应值合并,同时进行敏感性分析和偏倚评估。结果：纳入符合条件的相关文献5篇,共计ADHD 258例,对照138例。血清铁蛋白与儿童ADHD发生关系的研究间存在异质性（P=0.003）,采用随机效应模型进行分析,结果合并OR值（95%CI）为-23.09（-33.06～-13.13）, P＜0.00001。各研究的敏感性分析较稳定且无发表偏倚。结论：血清铁蛋白水平与儿童ADHD的发生有关。     

Hepcidin-to-ferritin ratio: A potential novel index to predict iron overload-liver fibrosis in ß-thalassemia major

ObjectivesWe aimed to determine a threshold cutoff for hepcidin, ferritin, and the hepcidin-to-ferritin ratio in the diagnosis of liver fibrosis caused by iron overload in chronic hepatitis C virus (HCV)-free ß-thalassemia major patients .MethodsThis 1:1-matched case-control study included 102 individuals (3–30 yr.); 51 ß-thalassemia major patients with iron overload , and 51 apparently healthy individuals.ResultsThe highest areas under the receiver operating characteristic curves (AUC-ROCs) for the diagnosis of patients vs. controls had overlapping 95% confidence intervals (CIs): serum hepcidin (0.758; 0.64–0.87;    P ? 0.001), serum ferritin (1.000; 1.00–1.00; P  ?0.001), and the hepcidin/ferritin ratio (1.000; 1.00–1.00; P ? 0.001). For differentiation of patients with liver fibrosis stages of F0–F1 vs. F2–F4 and F0–F1 vs. F3–F4, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) with P-values ? 0.001 were the only statistically significant parameters, while the AUC-ROCs of the hepcidin/ferritin ratio (0.631, P = 0.188 and 0.684, P = 0.098) exhibited 90% and 89.5% sensitivity, respectively, in staging liver fibrosis.ConclusionOur results showed that the hepcidin/ferritin ratio is as effective as the APRI and maybe a better predictor for the diagnosis of liver fibrosis and discriminating its stages, with excellent sensitivity and specificity compared to its components.     

促红细胞生成素、叶酸、维生素B12、铁蛋白检测在缺铁性贫血诊疗中的应用分析

探讨缺铁性贫血(IDA)发病机理,为诊断、治疗、预后判断提供依据.用化学发光免疫分析法,对240例缺铁性贫血患者、30名对照者及112例IDA患者在对症治疗和口服力蜚能、生血宁治疗一个疗程(30天)前后,体内血清中促红细胞生成素(EPO)、叶酸(FA)、维生素B12(vit B12)、铁蛋白(Fer)水平变化及EPO/Fer、vit B12/ FA比值的变化进行测定,并进行统计学分析.结果表明,缺铁性贫血组与对照组比较,EPO、FA、 vit B12、EPO/Fer、vit B12/ FA有显著性差异(P<0.05～0.001),EPO、Fer水平及EPO/Fer比值变化在IDA患者治疗前后比较呈正相关(r=0.875, t=4.256,P<0.001).缺铁性贫血虽主要是由铁的供应和贮存铁缺乏引起,但与体内EPO、FA、Fer、vitB12水平变化密切相关,EPO、Fer水平及其比值变化与IDA的发生、发展、治疗和预后判断有一定关系,是诊断和鉴别IDA的重要指标.     

癌胚抗原、糖链抗原19-9、C-反应蛋白和铁蛋白联合检测对胰腺癌的诊断价值

目的探讨联合检测血清癌胚抗原(CEA)、糖链抗原19-9(CA19-9)、铁蛋白(Ferr)和C-反应蛋白(CRP)对胰腺癌和慢性胰腺炎的诊断价值。方法分别检测CEA、CA19-9、CRP和Ferr在正常对照组、慢性胰腺炎和胰腺癌患者的含量。结果四项指标单独区分正常对照组和胰腺癌组的诊断能力依次为Ferr、CRP、CA19-9和CEA,Ferr的诊断灵敏度和特异性分别为96.60%和96.20%;区分慢性胰腺炎组和胰腺癌组的诊断能力依次为Ferr、CA19-9、CRP和CEA,Ferr的诊断灵敏度和特异性分别为71.20%和87.50%。利用二元logistic回归分析多项指标联合检测的诊断价值,当Ferr和CEA联合检测时,其曲线下面积为0.866,高于CEA、CA19-9、CRP和Ferr单独检测,诊断的灵敏度和特异性分别为74.60%和91.70%。结论 Ferr和CEA联合检测,可提高区分慢性胰腺炎和胰腺癌的诊断能力。