Syndecan-1和E-Cadherin蛋白在散发性大肠癌中的表达及意义

目的　探讨Syndecan -1和E -Cadherin两种蛋白在散发性大肠癌中的表达及意义。方法　应用免疫组化SP法 ,检测 5 2例散发性大肠癌和 18例正常大肠黏膜组织中Syndecan -1和E -Cadherin蛋白的表达。结果　Syndecan -1和E -Cadherin在散发性大肠癌和正常大肠黏膜组织的阳性表达率分别为 65 3 8%、10 0 %和 61 5 4%、10 0 % ,两者在散发性大肠癌和正常大肠黏膜组织的阳性表达率差异均有显著性 (P <0 0 1)。Syndecan -1和E -Cadherin的低表达与散发性大肠癌的浸润深度、淋巴结转移、Dukes分期显著相关 (P <0 0 5 ) ,其中Syndecan -1的低表达还与肿瘤的分化程度密切相关 (P <0 0 5 )。Syndecan -1和E -Cadherin在散发性大肠癌的表达呈正相关。结论　Syndecan -1和E -Cadherin的表达下调可能在散发性大肠癌的侵袭转移中起着重要作用 ,联合检测Syndecan -1和E -Cadherin对散发性大肠癌的预后判断有参考价值。     

乳腺癌E-Cadherin的表达与术野脱落癌细胞关系的研究

研究粘附分子E-Cadherin(E-CD)在乳腺癌中的表达及其与临床病理特征及术野脱落癌细胞的关系，以求更深入地探讨乳腺癌侵袭和转移的机制，为乳腺癌临床的诊治提供参考。 方法采用免疫组织化学ABC法，研究E-CD在52例乳腺浸润性导管癌组织中的表达强度。 结果52例乳腺癌中ECD正常表达者15例(28.9％)，减弱表达者14例(26.9％)，表达缺失23例(44.2％)，，总异常表达率为71.1％。E-CD的表达与组织学分级显著相关，异常表达率Ⅰ级(36.4％)明显低于Ⅱ级(75％)和Ⅲ级(92.3％)(P     

黏液素和上皮钙黏附蛋白在子宫内膜癌中的表达及其临床意义

目的探讨MUC1和E-cadherin在增生期子宫内膜、子宫内膜单纯性增生、子宫内膜不典型性增生和子宫内膜样腺癌组织中的表达及意义。方法采用免疫组化Envision法检测30例增生期子宫内膜组织、36例单纯性增生内膜、50例不典型增生内膜及55例子宫内膜样腺癌组织中MUC1和E-cadherin蛋白表达。结果从增生期子宫内膜-宫内膜单纯性增生-宫内膜不典型增生-子宫内膜样腺癌,MUC1和E-cadherin的正常表达率逐渐降低,异质表达率逐渐升高（P=0.000）。MUC1蛋白表达与年龄有一定关系（P=0.044）、与组织学分级无关（P=0.678）。E-cadherin蛋白表达与年龄变化及组织学分级无关（P=0.610,P=0.125）。MUC1和E-cadherin蛋白表达在子宫内膜样腺癌组织中呈正相关关系（γ=0.2897,P=0.0317）。结论 MUC1和E-cadherin蛋白异质表达参与了子宫内膜样腺癌的发生发展过程,对子宫内膜癌的诊断有一定意义。     

前列腺素E2对人肝细胞癌HepG2细胞E-cadherin表达的影响及其分子机制

目的:研究前列腺素E2(PGE2)对人肝细胞癌HepG2细胞E-cadherin表达的调节作用及其分子机制,探讨其与肝癌侵袭转移的关系,为肝癌的治疗提供新的研究思路.方法:COX-2瞬时转染人肝细胞癌HepG2细胞,Western blot实验检测其E-cadherin表达的变化;PGE2及PI-3K抑制剂LY294002处理人肝细胞癌HepG2细胞,应用Western blot和RT-PCR分别测定肝癌细胞内E-cadherin蛋白水平和mRNA水平的变化,并观察60 min内Akt磷酸化水平的变化.结果:COX-2过表达转染HepG2细胞后,E-cadherin的表达明显降低;5、10、20μmol/L PGE2处理HepG2细胞24 h后,E-cadherin表达水平与对照组比较分别下降了 22.31%、36.3%、47.83%(P<0.05);20 μmol/L PGE2处理HepG2细胞24、48、72 h,E-cadherin蛋白和mRNA的表达与对照组比均明显降低(P<0.01).PI-3K抑制剂LY294002能部分阻断PGE2对E-cadherin表达的调节作用;经PGE2处理的HepG2细胞Akt磷酸化水平显著升高,并且在15 min时达到最高.结论:PGE2可以抑制人肝细胞癌HepG2细胞中E-cadherin的表达,并很可能通过激活Akt信号转导通路而发挥作用.     

Disruption of E-Cadherin-mediated Cell Adhesion Systems in Gastric Cancers in Young Patients

The aim of this study was to the pathogenetic backgrounds of early-onset gastric cancers. Mutations of the E-cadherin and β-catenin genes were analyzed by subjecting microdissected cancer cells and corresponding non-cancerous epithelial cells obtained from 9 gastric cancer patients under 35 years old to polymerase chain reaction-single strand conformation polymorphism analysis. Somatic, but no germline, E-cadherin gene mutations were detected in 6 (67%) of the patients. The cancer cells of 2 patients were exon 9-deleted E-cadherin molecule-immunoreactive. Neither somatic nor germline mutations in exon 3 of the β-catenin gene were observed in any patient. One patient lacked β-catenin immunoreactivity and the cancer cells of 6 others showed cytoplasmic β-catenin immunoreactivity. The E-cadherin-mediated cell adhesion system in the cancer cells of all the patients examined appeared to be disrupted, indicating that somatically acquired dysfunction of this system plays an important role in early-onset diffuse-type gastric cancers. Helicobacter pylori infection was observed in 6 (67%) of our 9 patients, an incidence higher than the average in young Japanese individuals. Thus, early-onset gastric cancers may be attributable to environmental factors such as Helicobacter pylori infection.     

E-钙黏附素的表达在妊娠期高血压疾病发病中的作用

目的　探讨胎盘滋养细胞E 钙黏附素 (E Cadherin)的异常表达在妊娠期高血压疾病发病中的作用。方法　收集 2 0 0 0年 7月至 2 0 0 1年 11月新疆医科大学附一院 4 0例妊娠期高血压疾病 (研究组 )和 4 3例正常妊娠孕妇 (对照组 )的胎盘组织 ,采用免疫组化法测定两组胎盘组织E 钙黏附素的表达 ,分析其表达异同。结果　研究组胎盘E 钙黏附素的表达明显高于对照组 ,差异有显著性 (P <0 0 1)。结论　 (1)胎盘组织E 钙黏附素的异常表达导致胎盘浅着床 ,可能是妊娠期高血压疾病发病的始发原因之一。 (2 )E 钙黏附素的异常表达与妊娠期高血压疾病不良妊娠结局有关。     

Expression of E-Cadherin and the PTEN Gene in Relation to Invasion and Metastasis of Gastric Carcinomas

Objective To observe the expression of PTEN and E-Cadherin in gastric carcinomas (GCs): and to investigate the relationship between their expression and the pathology and prognosis of patients with GC. Methods The proposed markers were detected inmmunohistochemicaily by using the SABC method in 100 post-operated specimens of GC. The results were statically analyzed by the chi-square and log rank tests. Results Both E-Cadherin and PTEN proteins were expressed in noncancerous rnucosa. They were reduced or lost in GCs. The abnormal rate of expression of E-Cadherin was 42.0%. The decreased rate of expression in the diffuse-type GC (48.6%) was significantly higher than in the intestinaltype GC (26.7%, P< 0.05). The abnormal expression of E-Cadherin closely correlated to the depth of invasion (P< 0.05). The degree of loss of the PTEN protein was 59.0% in GCs. In the diffuse-type GC, the rate of toss of PTEN was (65.7%) which was significantly higher than that in the intestinal-type GC (43.3%,P< 0.05). The rate of loss of PTEN (64.5%) in GCs with lymph node metastasis was significantly higher than that in GCs without metastasis (41.7%, P< 0.05). The prognosis of patients with a loss of PTEN protein was worse than the patients with positive expression of PTEN (P=0.0066). E-Cadherin was normally expressed in 65.9% of GCs with positive expression of PTEN. Conclusion The loss of E-Cadherin and PTEN markers correlated with infusion and metastasis of GC. The expression of PTEN showed a close relationship to the prognosis of patients. Detection of the 2 markers together aided in the correct prediction of the prognosis of the GC patients and provided information for clinical treatment.     

宫颈病变患者TWIST与E-Cadherin表达水平的相关性分析

为了分析宫颈病变患者TWIST与上皮型钙黏附素(E-Cadherin)的表达水平与宫颈癌发生发展相关性。收集宫颈癌标本74例、同期完整CIN标本35例及正常宫颈组织22例,免疫组化染色法检测TWIST与E-Cadherin的表达水平。结果显示,与正常宫颈、CIN组织相比,TWIST在宫颈癌组织中的阳性表达率增高,E-Cadherin表达降低,这种表达差异与宫颈癌间质浸润深度和淋巴结转移有关(P<0.05),且TWIST与E-Cadherin在宫颈癌组织中表达水平呈负相关(r=-0.511,P<0.05)。结果提示TWIST表达增加与E-Cadherin表达降低可能与宫颈癌的发生发展及肿瘤细胞浸润转移密切相关。     

前列腺癌组织P504S、E-Cadherin和bcl-2蛋白表达及意义

目的分析前列腺癌中P504S、E-Cadherin和bcl-2蛋白的表达情况,探讨其在前列腺癌诊断及预后判断中的意义。方法应用免疫组织化学(Max Vision)法检测45例前列腺腺泡腺癌、6例前列腺高级别上皮内肿瘤及10例前列腺增生组织中P504S、E-Cadherin和bcl-2蛋白的表达情况。结果 P504S蛋白阳性率前列腺癌组(84.4%)明显高于前列腺高级别上皮内肿瘤组(50.0%)和前列腺增生组(0%)(P<0.05);E-Cadherin蛋白阳性率前列腺癌组(42.2%)和前列腺高级别上皮内肿瘤组(33.3%)明显低于前列腺增生组(100%)(P<0.05);bcl-2蛋白阳性率前列腺癌组(60.0%)明显高于前列腺高级别上皮内肿瘤组(16.7%)和前列腺增生组(20.0%)(P<0.05)。前列腺癌Gleason 8～10分组E-Cadherin蛋白的表达率(31.8%)明显低于2～4分组(83.3%)(P<0.05);8～10分组bcl-2蛋白的表达率(68.2%)明显高于2～4分组(33.3%)(P<0.05)。前列腺癌Gleason8～10分组(18.2%)和5～7分组(17.6%)E-Cadherin蛋白的强阳性表达率明显低于2～4分组(83.3%)(P<0.05)。前列腺癌组P504S蛋白阳性同时E-Cadherin蛋白阴性的比率(51.1%)明显高于前列腺增生组(0%)(P<0.05);前列腺癌组P504S蛋白阳性同时bcl-2阳性的比率(57.8%)明显高于前列腺增生组(0%)(P<0.05)。结论 P504S高表达、bcl-2高表达有助于前列腺癌的诊断;联合检测P504S和E–Cadherin,P504S和bcl-2的表达,可以提高诊断前列腺癌的准确性。     

大肠癌组织蛋白酶D和E-Cadherin的表达及意义

目的 检测组织蛋白酶D(Cathepsin D.简称Cath-D)、E-Cadherin在大肠癌中的表达及其临床意义,探讨其在大肠癌中的作用及两标记物间的相关性.方法 应用免疫组化方法研究32例大肠癌手术标本和相应癌旁的正常组织标本及12例大肠腺瘤组织中Cath-D,E-Cadherin的表达情况.结果 ①Cath-D在大肠癌和正常组织及腺瘤组织中的阳性表达率分别为71.9%,8.3%,0;E-Cadherin分别为34.4%,75%,和83.3%,两者在肠癌组织与腺瘤、正常组织表达率均有显著差异(P＜0.01).②两者均与大肠癌的浸润深度、淋巴结转移、肝转移及Duke's分期有密切关系,E-Cadherin与肿瘤分化程度亦相关.③Cath-D与E-Cadherin无显著相关性.结论 Cath-D与E-Cadherin在大肠癌的发生发展及转移中起重要作用,可作为预测预后的指标.