14-3-3蛋白与细胞凋亡

14 3 3蛋白是Moore和Pere于 196 7年发现的 ,当时他们认为 14 3 3蛋白是脑所特有的一种酸性蛋白 ,并依据其在DEAE 纤维素色谱上的组分数以及在淀粉凝胶电泳中的迁移位置而命名为 14 3 3蛋白。但随后的大量研究表明14 3 3蛋白在所有的真核细胞中均有表达 ,是一个调节蛋白家族 ,能与许多功能不同的信号蛋白结合 ,例如蛋白激酶Raf 1、KSR 1、Bcr、Ca2 +/钙调蛋白激酶、PKC、蛋白磷酸酶cdc2 5c、促凋亡蛋白BAD、抗凋亡蛋白A2 0 、跨膜蛋白受体GA等。这就说明 14 3 3蛋白在信号传导、细胞周期调控、细胞凋亡等过程中具有极其重要的…     

Human immunodeficiency virus 1 favors the persistence of infection by activating macrophages through TNF

Macrophages play a major role in HIV-1 persistence. In the present paper, we demonstrate that the absence of apoptosis in HIV-1-infected primary human monocyte-differentiated macrophages (MDM) correlates with an increase in anti-apoptotic (Bcl-2 and Bcl-x(L)) and a decrease in pro-apoptotic (Bax and Bad) proteins. This is associated with macrophage activation as shown by tumor necrosis factor (TNF) production and NF-kappaB activation upon infection. TNF production was shown to be involved in the upregulation of Bcl-2 and Bcl-x(L) because this increase was abolished by an anti-TNF anti-serum or an inhibitor of TNF synthesis. In parallel, inhibition of TNF production induced an increase in the number of apoptotic cells. Furthermore, using an inhibitor of NF-kappaB activation, we demonstrated that TNF-induced upregulation of Bcl-x(L) and Bcl-2 occurs, respectively, through a NF-kappaB-dependent and an NF-kappaB-independent pathway.     

人文医疗在阴式全子宫切除术联合阴道后壁修补术治疗子宫脱垂合并肠疝患者中的应用

目的探究人文医疗在阴式全子宫切除术联合阴道后壁修补术治疗老年子宫脱垂合并肠疝(阴道后壁膨出)患者中的应用效果。方法选择2018年1月至2019年12月期间天长市中医院收治子宫脱垂合并肠疝患者120例作为研究对象。全部入选病例均行阴式全子宫切除术联合阴道后壁修补术治疗,采用随机数字表法将病例分为对照组和观察组,各60例。对照组给予常规护理,观察组给予人文医疗护理。对比2组患者中文版知觉压力量表(CPSS)评分、抑郁自评量表(SDS)、焦虑自评量表(SAS)评分、心理弹性量表-简表(RS-14)、护理服务满意度、皮质醇(Cor)、心率及平均动脉压。结果护理前,2组CPSS、SDS、SAS及RS-14评分组间比较的差异无统计学意义(P>0.05);护理7 d后,2组CPSS、SDS、SAS及RS-14评分均较护理前降低,且观察组均低于对照组,差异有统计学意义(P<0.05)。观察组患者的满意度(96.67%)较对照组高(80.00%),差异有统计学意义(P<0.05)。观察组手术结束时2组患者的Cor、心率及平均动脉压较对照组低,差异有统计学意义(P<0.05)。结论人文医疗应用于子宫脱垂合并肠疝患者护理中,能够改善其不良情绪,缓解心理压力,减少手术的应激反应,提高患者对护理满意度。     

PTEN/PI3K信号转导途径与子宫内膜癌生物学行为的相关性

目的 :研究PTEN/PI3K信号转导途径与子宫内膜癌发生发展的关系。方法 :应用免疫组化和免疫印迹方法对 68例子宫内膜癌和癌前病变组织及 11例正常子宫内膜进行PTEN、p PKB及p Bad蛋白检测。结果 :1)PTEN蛋白的阳性表达率在正常增殖期子宫内膜组织中最高 ( 90 9% ) ,非典型增生组织中开始下降 ,在子宫内膜癌组织中明显降低 ( 4 9 1% ) ,三者之间比较 ,差异有统计学意义 ,P <0 0 1;2 )p PKB及p Bad与PTEN的阳性表达呈相反趋势 ,在非典型增生组织及内膜癌组织中p PBK及p Bad表达阳性率明显增加 ,差异均有统计学意义 ,P <0 0 1,P <0 0 5。在子宫内膜癌PTEN表达阴性组织中p PKB、p Bad蛋白染色积分均明显高于PTEN阳性组 ,P <0 0 5 ,P <0 0 1;3 )相关分析显示PTEN与p PKB及p Bad表达均呈负相关 ,r =-0 67,P <0 0 5 ;r =-0 65 ,P <0 0 5 ;4)PTEN、p Bad蛋白的阳性表达率在不同的临床分期、组织学分级及不同肌层浸润程度之间比较 ,差异均无统计学意义 ,P >0 0 5 ,而PTEN、p PKB只在临床分期间差异有统计学意义 ,P <0 0 5。结论 :PTEN/PI3K信号转导途径与子宫内膜癌的发生密切相关。伴随PTEN表达缺失 ,p PKB、p Bad阳性表达更加明显 ,提示PTEN蛋白表达可作为子宫内膜癌早期诊断指标和基因治疗靶点。     

叙事护理对肺癌免疫治疗患者心理弹性状况、健康信念及不良情绪的影响

目的:探究叙事护理对肺癌免疫治疗患者心理弹性状况、健康信念及不良情绪的影响。方法:选取2018年3月至2019年3月在该院行肺癌免疫治疗的患者70例,根据随机原则分为常规组和叙事组各35例。常规组患者行常规护理,叙事组患者在常规护理基础上实施叙事护理。比较两组患者干预前后心理弹性状况、健康信念及不良情绪。结果:干预前,...     

网瘾患者人格障碍的康复模式研究

目的 探讨改善网瘾患者人格障碍的有效措施,重建其健康人格,提高社会适应能力.方法 60例网瘾患者随机分为观察组(32例)和对照组(28例).两组均按常规药物治疗,对照组给予功能制护理,观察组给予8w的学校教育和医院护理管理相结合的矫治模式.两组采用卡氏16种人格量表(16PF)和症状自评量表(SCL-90)进行效果评定.结果治疗前两组16PF和SCL-90量表各因子分值无统计学差异(P＞0.05),治疗后观察组16PF和SCL-90量表各因子分值的改变均明显优于对照组(P＜0.01).结论学校教育和医院护理管理相结合的矫治模式能够有效地改善网瘾患者的人格障碍,提高他们的心理健康水平和社会适应能力.     

Evaluación de la efectividad de la versión en español de un curso de comunicación de malas noticias

IntroductionGood communication by health professionals is associated with better patient outcomes. The Program to Enhance Relational and Communication Skills (PERCS) has been adapted to other cultures. The objective of this study is to describe the effectiveness of the Spanish version of the PERCS program, from the perspective of the participants, at the end of the course and 6 months after the course.MethodsA process of cultural and epidemiological adaptation of the PERCS program to the reality of Peru was carried out. A qualitative and quantitative approach was used to evaluate the educational impact of the program.ResultsThirty-nine professionals participated voluntarily. Participants found it more difficult to communicate ominous diagnoses (48.15%). Anxiety is the most frequent emotion when faced with difficult communications (77.78%). Almost all (92%) stated that the course improved their preparation, and 88.9% that it reduced their anxiety. At 6 months, participants recognised the importance of honesty and empathy, as well as some steps in conducting the patient-centred interview.DiscussionAn intensive training program in difficult communication can be adapted to Spanish, and positively impact the skills, self-confidence, and self-perception of learning in Peruvian professionals, in relation to promoting communication focused on the needs of the patient and family.     

35例肺结核病患者不良心理状态的分析及护理对策

目的调查影响肺结核病人心理状况的因素,探讨如何实施心理护理。方法回顾性分析我院收治的35例结核病患者的临床护理资料,分析患者心理状态及总结护理经验。结果本组肺结核病人心理变化复杂,道德感、理智感比较成熟,但也存在着焦虑、消极、孤独等不良心理状态;经过合适的护理后,坚持住院痊愈14例,症状减轻出院20例,死亡1例。结论有针对性的实施心理护理对肺结核病人的治疗和预后具有重要意义。     

Risk factors for bad splits during sagittal split ramus osteotomy: a retrospective study of 964 cases

To identify the potential risk factors for bad splits, we calculated the incidence of bad splits from 484 patients with 964 cases of sagittal split ramus osteotomy (SSRO) and investigated the association between the occurrence of bad splits and risk factors such as gender, patients’ age, class of occlusion, unimaxillary or bimaxillary surgery, presence of the lower third molar, thickness of the ascending ramus, and the distance from the mandibular canal to the buccal cortical bone. The results showed that 40 sides (4.149%) with bad splits occurred in 36 patients (7.438%). The mean (SD) gap width from the canal to the buccal cortex for the bad split group, at 4.02 (1.20) mm, was narrower (p = 0.003; OR = 0.689; 95% CI = 0.538 to 0.882) than the normal split group 4.80 (1.72) mm. On the contrary, no statistical significance (p > 0.05) was detected between the patients with bad splits and those with normal splits for the other factors. In conclusion, SSRO patients with narrower distances from the mandibular canal to the buccal cortex were more prone to bad splits. More attention should be paid to patients with this risk factor during future surgeries.     

Halitosis: prevalence,risk factors,sources, measurement and treatment – a review of the literature

Halitosis, an offensive breath odour, has multiple sources and negative impacts on people’s social interactions and quality of life. It is important for health care professionals, including general physicians and dental professionals, to understand its aetiology and risk factors in order to diagnose and treat patients appropriately. In this study, we have reviewed the current literature on halitosis regarding its prevalence, classification, risk factors, sources, measurement and treatment.