张君教授治疗过敏性紫癜经验探析

张君教授提出：“热、毒、瘀、虚”是小儿过敏性紫癜的主要病机,在治疗上主张活血化瘀贯穿疾病始终,早期佐以清热解毒,中期滋阴凉血,后期益气摄血为辅。临床每获良效。此外,遵循“未病先防,既病防变”原则,防治疾病反复、进展。     

Prevention of ERK activation involves melatonin-induced G(1) and G(2) /M phase arrest in the human osteoblastic cell line hFOB 1.19

Melatonin regulates mitogen-activated protein kinase (MAPK) and Akt signaling pathways. The MAPK family mainly includes extracellular signal-regulated kinase (ERK), p38, and c-Jun N-terminal kinase (JNK). Our previous study documented that melatonin delays osteoblast proliferation; however, the mechanism of action of melatonin remains unclear. Here, we demonstrate that melatonin significantly inhibited phosphorylation of ERK but not p38, JNK, or Akt in a human osteoblastic cell line 1.19 (hFOB), as measured by western blot. The expression of ERK, p38, JNK, and Akt was not altered. PD98059 (a selective inhibitor of MEK that disrupts downstream activation of ERK) and melatonin alone, and especially in combination, significantly induced an antiproliferative effect, G(1) and G(2) /M phase arrest of the cell cycle, and downregulation of the expression at both the protein and mRNA levels of cyclin D1 and CDK4, related to the G(1) phase, and of cyclin B1 and CDK1, related to the G(2) /M phase, as measured by the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) method, flow cytometry after propidium iodide staining, and both western blot and real-time PCR, respectively. Moreover, the combination of PD98059 and melatonin synergistically and markedly augmented the action of either agent alone. Coimmunoprecipitation further confirmed that there was an interaction between phosphorylation of ERK and cyclin D1, CDK4, cyclin B1, or CDK1, which was weaken in the presence of melatonin or PD98059. These results suggest that the prevention of ERK activation is involved in melatonin-induced G(1) and G(2) /M phase arrest, and this inhibitory effect is potentially via the ERK, but not p38, JNK, or Akt, pathway.     

中药厌氧灵对腹腔感染所致内毒素血症治疗作用的研究

目的：观察厌氧灵对腹腔感染所致内毒素血症期患者的治疗价值。方法：２０例腹部外科入院患者,分为两组：（１）西医综合治疗组,ｎ＝１０;（２）厌氧灵＋西医综合治疗组,ｎ＝１０。在治疗前（０）,治疗生１、３、７ｄ分别观测、记录口才一般体征,清晨空腹采血测定血浆内毒素水平。内毒素测定采用鲎三肽偶氮显色法。结果：厌氧灵治疗组体温、外周 ＷＢＣ计数下人于对照组体温第３ｄ后有显著差异,Ｐ〈０．０５;排气时间早于对     

塔里木油田缝洞型碳酸盐岩油藏深井裸眼段挤水泥堵水技术实践

塔里木英买力油田缝洞型碳酸盐岩油藏深井裸眼段井径不规则,大裂缝、大溶洞发育,漏失严重,高温,超深,给挤水泥作业带来了困难。为提高挤水泥成功率及降低成本,根据油田地质特点和生产实践,采取平推注水泥浆、桥塞暂堵打塞等工艺,取得了很好的堵水效果,进一步挖掘了油气井潜力。     

Airway epithelial ITGB4 deficiency induces airway remodeling in a mouse model

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Enhancer and super-enhancer landscape in polycystic kidney disease

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