艳山姜挥发油抑制JNK1/2/3磷酸化对ox-LDL诱导的HAECs损伤的影响

目的:研究艳山姜挥发油对氧化低密度脂蛋白(ox-LDL)诱导人主动脉内皮细胞(HAECs)损伤的保护作用,并分析可能的作用机制。方法:实验分为空白组(无血清内皮细胞培养基),模型组(200 mg·L-1ox-LDL),艳山姜挥发油高剂量组(200mg·L-1ox-LDL+100μg·L-1艳山姜挥发油)和低剂量组(200 mg·L-1ox-LDL+10μg·L-1艳山姜挥发油)共4组。艳山姜挥发油预保护1 h,继续加入ox-LDL共同作用24 h,采用噻唑盐(MTT)法分析细胞存活率,苏木精-伊红(HE)染色法进行形态学观察;采用酶联免疫吸附法(ELISA)检测培养上清液乳酸脱氢酶(LDH)外漏量,Western blot分析c-Jun氨基末端激酶(JNK)1/2/3,p-JNK1/2/3蛋白表达,实时荧光定量聚合酶链式反应分析JNK1/2/3 mRNA表达水平。结果:艳山姜挥发油能显著提高HAECs的存活率,改善细胞的病理损伤,减少LDH外漏量,抑制JNK1/2/3蛋白磷酸化,但对JNK1/2/3蛋白和mRNA水平无显著影响。结论:艳山姜挥发油改善ox-LDL诱导的HAECs损伤,其作用机制与抑制JNK1/2/3蛋白磷酸化相关。     

方祝元教授运用潜阳育阴和血法治疗高血压肾损害的学术思想总结

肾脏是高血压损害的主要靶器官之一,而肾损害早期,若积极治疗,肾功能是可以逆转的,导师方祝元教授在精研历代医学论著的基础上,结合长期临床实践,以滋阴潜阳为主兼以和血为法,自制潜阳育阴颗粒治疗此病疗效显著。     

宽叶缬草对局灶性脑缺血后海马区C-Fos,C-Jun表达的实验研究

INTRODUCTIONStrokeisoneofthemostcommoncauseofdeathanddisablinganeurologicdisorderinChina．RecentresearchshowedthatChinesetraditionalmedicinecouldimprovetheoutcome，butthemecha－nismwasstillunclear犤１－３犦．Valerianofficinalsvar．latifolia（VOL）isoneofthemedicineinShennongjia，Hubeiprovince，whichcouldeliminateoxygen－derivedfreeradidicals，protectvascularendothe－lialcellsandinhibitproliferationofsmoothmusclecells．Thisex－perimentselectreversiblemiddlecerebralarteryocclusion（MCAO…     

杨群玉教授治疗肿瘤经验

杨群玉教授认为,肿瘤的形成原因有二：一是正虚邪实,正气虚弱,邪毒内生,积聚成块;二是饮食起居失常,毒邪外侵踞之而成积聚。治疗主张以固本扶正为本,强调顾护胃气的重要性,用药多用健脾益胃补气之品;遣方用药要根据病人体质辨证及根据肿瘤的不同阶段和病理变化辨证运用抗肿瘤中药;同时诊疗过程中应注重患者情志心理的疏导,适当予疏肝理气、健脾和胃之品,配合饮食调理。     

香砂六君子汤联合昂丹司琼治疗化疗后消化道不良反应50例疗效观察

目的观察香砂六君子汤治疗恶性肿瘤化疗后消化道不良反应恶心、呕吐、腹泻食欲不振的疗效.方法 将100例恶性肿瘤进行化疗之患者随机分为两组各50例治疗.对照组:单纯使用西药昂丹司琼、胃复安等治疗,治疗组:在对照组治疗基础上同时服用香砂六君子汤水煎剂.两组临床资料相同,观察治疗前后恶心、呕吐腹泻食欲不振变化情况及临床疗效.结果 总有效率(显效+有效率治疗组为96﹪,对照组60﹪.两组总有效率比较有显著差异(p<0.01).结论 香砂六君子汤联合昂丹司琼对治疗恶性肿瘤所致消化道不良反应可有效改善临床症状,有良好的疗效.     

Opioid μ-receptors as new target for insulin resistance

Type-2 diabetes is one of the fastest growing public health problems worldwide resulting from both environmental and genetic factors. Activation of μ-opioid receptor (MOR) could result in reversal of the impairment of insulin-stimulated glucose disposal in genetically obese Zucker rats via exercise training. This improvement of insulin resistance was associated with an elevation of circulating β-endorphin to ameliorate the post-receptor insulin signaling cascade, including downstream effectors of the phosphatidylinositol 3-kinase (PI3-kinase) signaling pathway. In insulin resistant rats, Loperamide treatment effected on the insulin receptor substrate (IRS)-1/PI3-kinase/Akt signaling cascade and subsequent insulin-stimulated glucose transport trafficking on skeletal muscle, which were all suppressed by MOR antagonism. In addition, induction of insulin resistance by the intake of high fructose is more rapid in MOR knockout mice than in wild-type mice. Improvements in insulin sensitivity through the peripheral MOR activation overcoming defects related to the post-receptor in IRS-1-associated PI3-kinase step have been defined. Opioid receptor activation, especially of the μ-subtype, may provide merits in the amelioration of defective insulin action. Atypical zeta (ζ) isoform of protein kinase C serves as a factor that integrates with peripheral MOR pathway and insulin signals for glucose utilization. The developments call new insights into the chemical compounds and/or herbal products that might enhance opioid peptide secretion and/or stimulate MOR in peripheral insulin-sensitive tissues to serve as potential agents or adjuvants for helping the glucose metabolism. In the present review, we update these topics and discuss the concept of targeting peripheral MOR pathway for the treatment of insulin resistance.     

马蹄内翻足功能锻炼器具的临床应用研究

目的：观察“马蹄内翻足功能锻炼器具”对于提高先天性马蹄内翻足临床疗效的促进作用。方法：经过杨氏疗法进行基础治疗后,配合运用马蹄内翻足功能锻炼器具治疗先天性马蹄内翻足90例共129R足。结果：129只足中,优48只,良75只,优良率95．3％。结论：马蹄内翻足功能锻炼器具能明显增强踝关节活动度,提高临床疗效。     

Cinnamophilin Isolated from Cinnamomum philippinense Protects against Collagen Degradation in Human Chondrocytes

To investigate the therapeutic potential of naturally occurring cinnamophilin against cartilage degradation and its action mechanisms, its effects on matrix metalloproteinase (MMP)‐1 and ?13 induction were examined in the human SW1353 chondrocytic cell line. Human chondrocytes (SW1353) were stimulated with interleukin (IL)‐1β, and then mitogen‐activated protein kinase (MAPK) and c‐Jun activations, inhibitory κB‐α (IκB‐α) degradation, and MMP‐1, and 13 expressions were assayed by a Western blot analysis. Cinnamophilin strongly inhibited MMP‐1 and ?13 induction in IL‐1β‐treated (30 ng/mL) SW1353 cells in a concentration‐dependent manner, and it also reduced MAPK family members including extracellular signal‐regulated kinase (ERK), p38 MAPKs, and c‐Jun N‐terminal kinase. Moreover, nuclear factor (NF)‐κB signaling activation through IκB‐α degradation, IκB kinase (IKK)‐α/β, and p‐65 phosphorylation was restored by cinnamophilin upon IL‐1β stimulation. Importantly, results showed that IL‐1β‐induced activation of phosphorylated (p)‐c‐Jun in chondrocytes was significantly inhibited by cinnamophilin. These results indicate that cinnamophilin inhibited MMP‐1 and ?13 expressions in IL‐1β‐treated chondrocytes through either NF‐κB or ERK/p38 MAPK downregulation and/or suppressing p‐c‐Jun pathways. Furthermore, these findings suggest that cinnamophilin may have the potential for chondroprotection against collagen matrix breakdown in cartilage of diseased tissues such as those found in arthritic disorders. Copyright © 2012 John Wiley & Sons, Ltd.     

“龙虎交战”与“子午补泻”针法的操作和综合应用

秉持溯源穷流精神,深入掌握“龙虎交战”针法,以发挥其住痛移疼即时效果.结合《针灸大成》提到的《内经》《难经》内容以及《针灸大成》所提各家论述,深入研究杨继洲对“龙虎交战”提出的“阴阳交贯之道”“消息阴阳九六中”“返复玄机随法取”等想法,以探究“龙虎交战”针法.“龙虎交战”针法结合“子午补泻”“提气法”与“九六补泻”法的观念来操作,则能让经气内外相通,进而使经气上下相接.将《针灸大成·三衢杨氏补泻》中的“龙虎交战”“子午补泻”与“提气法”三者的深层次精神综合运用,并参考张缙教授对“九六补泻”法的临床应用提示,则较能在临床实作中充分发挥出“龙虎交战”针法之住痛移疼即时效果.