地榆鞣质抗肝癌细胞SMMC—7721的MTT及FCM分析

探讨中药成分地榆鞣质的抗癌活性，并试图建立一种筛选抗癌药物的方法，方法：运用ＭＴＴ法测定ＳＴＭ及ＳＴＬ对体外培养人肝癌ＳＭＭＣ－７７２１细胞的杀伤率并通过流式细胞仪分析细胞分裂周期各时象ＤＮＡ变化。结果；ＳＴＭ与ＳＴＬ均有明显抗癌活性，与阴性对照组相比有显著差异，并具有剂量效应关系，ＳＴＭ，ＳＴＬ与ＭＭＣ联合用药抗癌活性增强，与单独用药组相比差异显著；     

附子理中丸方药的药物动力学研究

附子理中丸是中医治疗脾胃虚寒、脘腹冷痛、呕吐泄泻、手足不温的常用成药。本文通过小白鼠急性死亡实验,测得ip LD_(50)=42.4870g/kg;运用药物累积法对该复方方药进行了药物动力学研究。结果表明:附子理中丸在小鼠体内按一级动力学消除,呈二房室开放式模型分布。测得其t_(1/2)α=0.1922h,t_(1/2)β=11.2888h等动力学参数。阐明了该药的体内动态过程,为评价该药的内在质量及临床安全合理应用提供了参考依据。     

颅颌面形态有限元分析系统的初步建立

初步建立一个用于颅颌面形态变化研究的有限元分析系统。方法：程序用ＢｏｒｌａｎｄＣ语言编写，对临床常用的Ｘ线头颅定位片进行分析，将呆分析的头颅定位片描在硫酸纸上，用图形数值化仪把节点输入计算机。根据有限元分析法的原理，将头颅定位片上的颅颌面结构份割许多三角形单元，用应变张量来描述颅颌面形态变化。     

建立模拟功能状态下的下颌骨三维有限元模型

本文在三维影像重建和三维有限元分析技术的基础上，建立了正常人和颞下颌关节疾病患者正中咬合时下颌骨的三维有限元模型。下颌骨螺旋ＣＴ和有限元模型三维重建影像的几何相似性良好，加载方式符合生理状况，比较真实地反映和模拟了功能状态下颞下颌关节与牙合的受力情况。为对颞下颌关节在各种状况下的生物力学行为进行分析和研究创造了条件。     

CT对眼球异物的诊断及定位

本文比较了11例眼球异物CT与X线影像，并经手术摘出异物证实，CT眼球异物检出及定位准确性均优于X线，尤其对球壁异物的定位，而且能显示断层眼球壁轮廓，具有直观效果，CT异物影像比异物明显扩大，应警惕CT伪影可能使球壁异物定位发生误差，CT目前尚不能完全取代眼球异物常规X线检查。     

Die Durchblutung handgenähter und geklammerter Colonanastomosen

Zusammenfassung In einer tierexperimentellen Studie wurde die Durchblutung nach Anlage einer handgenähten und geklammerten End-zu-End-Anastomose am Schweinedickdarm verglichen. Bei keinem der 15 Tiere trat ein Nahtleck auf. Im Bereich handgeknoteter Fäden konnten einzelne umschriebene minderdurchblutete Gebiete festgestellt werden, die am 7. und 14. postoperativen Tag deutlich sichtbar wurden, wenn die Anastomosen vermehrt vascularisiert waren. Bei den Klammernähten wurden solche Ausfälle der Gewebsdurchblutung nicht gesehen. Die intramuralen Arterien zogen unbehindert durch die B-förmig gestalteten Klammern hindurch. Ein unauffälliges Gefäßmuster und eine abgeschlossene Anastomosenheilung wurden bei den Klammernähten nach 3 Wochen und bei Handnähten nach 4 Wochen beobachtet.

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Blood supply of stapled and sutured colonic anastomoses

Summary The blood supply of end-to-end staple and suture lines in the pig colon was compared. In 15 operated animals there was no anastomotic leak. Sporadic circumscribed areas of restricted circulation could be noticed in hand-sewn anastomoses. This effect was marked on the 7th and 14th postoperative day, when vascularisation of the anastomoses was increased. Such lack of vascular supply was not seen in stapled anastomoses. The intramural arteries passed through the B-shaped staples without hindrance. An unremarkable vascular pattern and a completed healing of the anastomoses were observed after 3 weeks in staple lines and after 4 weeks in suture lines.

Herrn Prof. Dr. Dr. h.c. F. Stelzner zum 65. Geburtstag gewidmet     

Waiting: the experience of persons in a critical care waiting room.

The purposes of this study were to discover the essences of the experience of waiting, to provide new knowledge about what it is like to wait, and to contribute to nursing knowledge. Participants were 12 persons who were family members or friends of persons in an adult critical care unit. The Parse research method was used to answer the research question: What is the structure of the lived experience of waiting? The central finding of this study was this structure: The lived experience of waiting is a vigilant attentiveness surfacing amid an ambiguous turbulent lull as contentment emerges with uplifting engagements. The structure is discussed in relation to nursing knowledge and in relation to how it can inform future research and practice. © 2002 John Wiley & Sons, Res Nurs Health 25:58–67, 2002.     

有抑郁症史者想象力的研究

实验组为45名缓解的内源性抑郁症患(男25例,女20例)。对照组为15名缓解的双相障碍患者(男8例,女7例),71名正常人(男41例,女30例)。利用作者建立的想象结局法发现:实验组想象力总分显著低于正常人组,特别是当涉及不愉快事件的测题时,单项分亦显著低于正常人。双相障碍组总分亦显著低于正常人。实验组有关性内容的比率得分显著低于正常人。     

尼氏小体染色方法的改进及其在神经病理学研究中的应用

组织或细胞的染色在病理学诊断、科学研究和教学工作中,都具有非常重要的意义和使用价值。组织切片染色的质量好坏对于医学诊断,科研和教学至关重要。为了更好的研究神经组织,使医学诊断、科研和教学工作更为方便,本文对Toluidine Blue(甲苯胺蓝)染色方法做了一些改进。在传统的甲苯胺蓝染色过程中,仅考虑对细胞核和尼氏小体进行染色,未考虑细胞浆和其他细胞器:而改进后的甲苯胺蓝染色方法在甲苯股蓝染色后用伊红再染色,既考虑对细胞核和尼氏小体进行染色,也对细胞浆进行了染色。结果显示传统的Toluidine Blue染色结果光镜下观察,细胞核和尼氏小体都可见,即尼氏小体为深蓝色,细胞核为蓝色,染色背景为淡蓝色;改进后的染色结果光镜下观察,尼氏小体为紫蓝色,细胞核为蓝色,染色背景为粉红色。可见,改进后的染色方法染出的组织切片比传统的要清晰、美观。随着科学技术的飞速发展,病理学的研究也随之发展,病理技术势必进一步提高,来适应科技的进步和医学的发展。改进的尼氏小体染色法能够使脑组织切片更清楚观察,更有利于医学工作者对神经组织及尼氏小体的研究。     

A One–Step, Operator–Independent Method for Isolating Islets of Langerhans from the Porcine Pancreas

Abstract: Large–scale isolation of islets of Langerhans is one of the major obstacles in islet transplantation. Until now, isolation methods relied on enzymatic digestion, the duration of which relies on a decision dictated by the operator's experience. This approach has always hindered development of an automated method. The aim of this study was to develop a one–step method based on complete digestion of the pancreas. The original aspect of the technique (derived from the Ricordi method) is use of the University of Wisconsin (UW) solution in the digestion medium and a continuous flow collagenase processing circuit with local cooling and rewarming to allow tissue digestion to proceed at 37°C while settling of the cell suspension takes place at 4°C. A stopcock system permits the alternate use of two settling chambers so that while one is in the circuit, the other can be removed for cen–trifugation, resuspension of the crude islet preparation in collagenase in free UW solution, and further purification in a density gradient system. Ten experiments were performed, and 545, 750 ± 48, 670 purified pig islets were obtained per totally digested pancreas. Histological studies showed cell integrity. Insulin secretion in response to double glucose stimulation under perfusion conditions demonstrated the functional viability of the isolated islets. In conclusion, this one–step method makes it possible to obtain a high number of viable islets of Langerhans in the absence of any decision by an operator, and it should therefore provide basis for an automated method.