不同肥胖测量指标与成年女性血清C反应蛋白的关联研究

目的 探讨不同的肥胖测量指标对中国成年女性血清C反应蛋白(CRP)的关联。方法 数据来源于成都市双流区城镇乳腺癌筛查项目基线调查及随访调查,共纳入441名成年女性。对研究对象开展问卷调查、体格检查和实验室检测。采用多因素logistic回归模型、两水平线性混合效应模型和限制性立方样条模型探讨不同肥胖测量指标与成年女性血清CRP的线性和非线性关联。结果 BMI、腰围和体脂率每增加1个单位,成年女性血清CRP升高即慢性低度炎症状态加重的风险分别增加16.5%、5.0%和11.1%(P<0.05)。BMI和体脂率与血清CRP之间存在非线性关联,以BMI=24.0 kg/m²为参考点,当BMI>24.0 kg/m²时,血清CRP水平随BMI的增加而增加;以体脂率=30%为参考点,当体脂率>30%时,血清CRP水平随体脂率的增加而增加。结论 BMI反映的整体肥胖与成年女性血清CRP关联最强,体脂率反映的体脂含量与血清CRP关联强度次之,腰围反映的中心性肥胖与血清CRP关联相对最弱。BMI>24.0 kg/m²和体脂率>30%的成年女性是产生肥胖相关炎性表现的高危险人群。     

高效液相色谱法测定盐酸莫西沙星原料药中的有关物质#br#

目的 建立盐酸莫西沙星原料药有关物质的HPLC测定方法。方法 采用Agilent Eclipse Plus C18色谱柱(4.6mm×250mm, 5μm)对有关物质A~H进行定量分析,以0.1%三乙胺溶液(用磷酸调节pH值至3.5)-乙腈(70:30)为流动相,流速1.0mL/min,检测波长293nm(检测杂质A、B、C、D、F),280nm(检测杂质G和H),243nm(检测杂质E),柱温30℃,进样量10μL。结果 盐酸莫西沙星与各杂质峰及各杂质峰之间的分离度均大于1.5,杂质A~H的线性范围分别是0.153~1.534、0.506~1.517、0.252~1.510、0.393~1.474、0.486~1.458、0.153~1.526、0.095~1.426和0.160~1.602μg/mL,各杂质在线性范围内均与峰面积成良好的线性关系(r>0.99),杂质A~H的检测限分别为0.0511、0.1517、0.1006、0.1474、0.2430、0.0509、0.0285和0.0481μg/mL,定量限分别为0.1534、0.5056、0.2516、0.3932、0.4859、0.1526、0.0950和0.1602μg/mL,分别加入80%、100%和120%指标浓度的杂质考察回收率,结果各个杂质的回收率均在80%~120%范围内,定量限和回收率的RSD均符合验证要求。3批样品有关物质测定的结果显示,已知杂质的含量均低于0.1%。结论     

正交设计优选砂炒牛蒡子的炮制工艺

目的:优选砂炒牛蒡子的最佳炮制工艺.方法:采用正交试验法考察炮制温度、炒制时间、砂用量等影响因素,以砂炒牛蒡子中牛蒡苷含量、爆裂率和煎出率作为评价指标,综合评分优选其工艺条件.结果:优选出最佳炮制工艺为:砂用量为药物重量的12倍,砂炒温度200℃,炒制时间4 min.结论:该炮制工艺可靠、爆裂率高、成品酥脆、质量好,可...     

Development of a lyophilized soybean paste certified reference material for the analysis of ochratoxin A

A certified reference material (CRM) [²KRISS CRM # 108-10-018] for the analysis of ochratoxin A (OTA) in doenjang (fermented soybean paste and popular food in Korea) was produced to ensure the reliability of analytical results in testing laboratories. A home-made doenjang was chosen as a raw material after testing its OTA level. The raw material was freeze-dried, pulverized, sieved and homogenized. An isotope-dilution-liquid chromatography/tandem mass spectrometric method (ID-LC/MS/MS) which was previously developed and validated in this laboratory was used as a higher-order reference method for characterization, homogeneity studies, and short-term stability studies. The CRM had good between-bottle homogeneity with 0.56% relative standard deviation among 10 selected units. The stability of the CRM at −70 °C (the storage condition in our laboratory) and at −20 °C (the possible storage temperature at user sites) were tested for up to 8 months. No change in the OTA content was observed within the measurement uncertainty. The stability of the CRM at room temperature (for regular use and transportation) was also tested and confirmed. The certified value was (49.50 ± 1.17) μg/kg, where the expanded uncertainty was in the confidence level of 95%.     

Right sided spleen laying retro-duodenal: A case report and review of the literature

IntroductionUnlike left sided accessory spleen that are seen in 10–30% of cases at autopsy, cases of right accessory spleens are extremely rare. This congenital body of healthy splenic tissue simulates tumors from neighboring organs and presents a challenge in formulating a differential diagnosis.Presentation of caseWe present the case of a patient whose CT scan of the abdomen showed a large mass, 11 × 8 cm, arising retro-duodenal and lying just anterior to the right kidney. To the best of our knowledge, this is the only case where the accessory spleen was found retro-duodenal, directly anterior to the kidney and completely separate from the supra-renal gland. The chief complaint of the patient was right upper quadrant pain, radiating to the back, and colicky in nature. The patient was diagnosed with duodenal gastro-intestinal stromal tumor and a retro-peritoneal sarcoma. The mass was removed via a Kocher’s incision and immunohistological examination showed that it was a right sided accessory spleen. The patient’s left sided spleen appeared normal.DiscussionEfforts to distinguish an accessory spleen from a retroperitoneal tumor with available scans, percutaneous biopsy or biochemical tests are inconclusive. Differential diagnosis between a retroperitoneal tumor and an accessory spleen can only be made after surgical exploration.ConclusionThis case highlights the fact that surgeons should consider the possibility of an accessory spleen when making a differential diagnosis of retroperitoneal tumors.     

Assessment of safety and reproductive performance after vaccination with a modified live-virus PRRS genotype 1 vaccine in pregnant sows at various stages of gestation

The objective of the present study was to assess safety and efficacy of a new modified live-virus porcine reproductive and respiratory syndrome (PRRS) genotype 1 vaccine in pregnant sows at various stages of gestation under field conditions. A total of 505 sows and gilts were allocated to two treatment groups and maintained in separate facilities. Animals of group 1 were vaccinated with a commercial modified live genotype 1 PRRSV vaccine (control product, CP), while animals of group 2 were immunized with a new modified live genotype 1 PRRSV vaccine (investigational veterinary product, IVP) (ReproCyc® PRRS EU, Boehringer Ingelheim Vetmedica GmbH). Injection site reactions were noted to be significantly less frequent in the IVP group compared to the CP group for pain (p = 0.039), redness (p = 0.030), heat (p = 0.016) and swelling (p = 0.002). The mean total number of piglets alive at weaning did not differ significantly between both study groups (10.6 vs. 11.0, p = 0.375). However, pre-weaning mortality was significantly higher (p = 0.005) in piglets from the CP group (14.1% vs. 10.9%). Analyses of reproductive performance data for both groups did not result in statistically significant differences between CP group and IVP group for number of piglets alive (12.7 and 12.6, respectively), healthy live (11.9 and 11.8), weak (0.7 and 0.5), stillborn (1.0 and 0.8) and mummified piglets (0.3 and 0.2) per litter. No differences were detected between both groups for piglet birth weights, while body weights at weaning (7.2 kg vs. 6.6 kg, p = 0.026) and average daily gain (0.2445 kg vs. 0.2211 kg, p = 0.037) were significantly higher in piglets from the IVP group. In conclusion, the administration of a single dose of ReproCyc® PRRS EU to sows and gilts at various stages of gestation confirmed non-inferiority to a commercial PRRS vaccine regarding safety and efficacy parameters under field conditions.     

环境水样中痕量铜的浊点萃取-火焰原子吸收光谱测定法

目的建立环境水样中痕量铜的浊点萃取(cloud point extraction,CPE)-火焰原子吸收光谱(flame atomic absorption spectrometry,FAAS)测定法。方法样品在p H 9.5的条件下,加入0.4 ml的1 mmol/L 2-(5-溴-2-吡啶偶氮)-5-二乙氨基酚(5-Br-PADAP)溶液,0.1%氯化钙溶液0.1 ml,5%(W/V)Triton X-114溶液0.8 ml,40℃加热15 min后离心,采用火焰原子吸收光谱法进行检测。结果在2~240μg/L的线性范围内,所得回归方程为A=0.002 7c+0.024 6,r=0.995 8。以3倍信噪比计算,方法的检出限为0.62μg/L,富集倍数为36.58倍,平均加标回收率为96.28%~98.08%,RSD为1.67%~3.13%。结论该方法简单、灵敏,具有良好的重现性,适用于环境水样中痕量铜的测定。