小陷胸汤加味含药血清对人脐静脉内皮细胞分泌NO/ET-1的调节作用

目的:探讨小陷胸汤加味中药方对血管内皮细胞的保护作用。方港:建立ox-LDL损伤人脐静脉内皮细胞株(ECV-304)模型,用小陷胸汤加味含药血清处理模型,并用放免和硝酸酶还原法在药物干预6h和24h后检测细胞上清液中ET-1和NO含量。结果:100 μg/ml的ox-LDL可损伤血管内皮细胞并导致其分泌NO和ET-1功能失调,小陷胸汤加味含药血清通过影响NO/ET-1的分泌而明显改善此失调状态。结论:小陷胸汤加味中药通过调节NO/ET-1水平显著拮抗ox-LDL对血管内皮细胞损害,具有防治AS的作用。     

Predicting Emotional Response to Unsuccessful Fertility Treatment: A Prospective Study

The predictive value of a comprehensive model with personality characteristics, stressor related cognitions, coping and social support was tested in a sample of 187 nonpregnant women. The emotional response to the unsuccessful treatment was predicted out of vulnerability factors assessed before the start of the treatment. The results indicated the importance of neuroticism as a vulnerability factor in emotional response to a severe stressor. They also underlined the importance of helplessness and marital dissatisfaction as additional risk factors, and acceptance and perceived social support as additional protective factors, in the development of anxiety and depression after a failed fertility treatment. From clinical point of view, these results suggest fertility-related cognitions and social support should receive attention when counselling women undergoing IVF or ICSI treatment.     

胰岛素对牛胸主动脉内皮细胞血管内皮生长因子及其受体表达的影响

目的:评价胰岛素对培养的牛胸主动脉内皮细胞血管内皮生长因子(VEGF)及其受体表达的影响。方法: 取新生的小牛胸主动脉,做血管内皮细胞原代及传代培养,取4-6代培养细胞分组,应用不同浓度的胰岛素(30 mU/L、300 mU/L、3 000 mU/L)干预培养过程,48 h后应用免疫组化法测定内皮细胞VEGF及其受体(flt-1、flk-1/KDR)的表达水平。结果: 低浓度胰岛素组(30 mU/L、300 mU/L)内皮细胞VEGF表达明显高于不用胰岛素组(P<0.01);高浓度组(3 000 mU/L)内皮细胞VEGF表达明显低于不用胰岛素组(P＜0.05);各组内皮细胞VEGF受体(flt-1及flk-1/KDR)的表达无明显差异(P＞0.05)。结论: 低浓度胰岛素促进小牛主动脉血管内皮细胞VEGF表达;高浓度胰岛素可抑制血管内皮细胞VEGF表达;胰岛素对小牛主动脉血管内皮细胞VEGF受体(flt-1、flk-1/KDR)的表达无直接影响。     

Monoclonal antibodies to human secretory "pregnancy-associated endometrial alpha 1-globulin," an insulin-like growth factor binding protein: characterization and use in radioimmunoassay, Western blots, and immunohistochemistry

Monoclonal antibodies were raised against pregnancy-associated endometrial alpha 1-globulin (alpha 1-PEG), a 32 KD insulin-like growth factor binding protein (IGF-BP), which represents a major secretory product of the human decidualized endometrium during pregnancy. This class of IGF-BP has been implicated in the modulation of action, inhibitory and stimulatory, of insulin-like growth factors. Immunization with the protein purified from pregnancy endometrium resulted after myeloma fusion in the isolation of six hybridoma clones and the antibodies produced were characterized. The Ka of the antibodies ranged between 4.75 x 10(9) M-1 and 0.7 x 10(8) M-1. In Western blots all monoclonal antibodies reacted with purified protein of molecular weight 32 KD and specifically detected this IGF-BP species in culture medium and cytosolic extracts of pregnancy endometrium and amniotic fluid. The monoclonal antibodies appear to define three epitope-bearing regions as evidenced by their reactivity to polypeptide fragments of the protein. After synthesis and secretion by tissue explants in vitro the protein is susceptible to cleavage into fragments possessing different monoclonal antibody-defined reactivity. Employing immunohistochemical techniques the protein was principally localized to decidual cells in tissue sections of pregnancy endometrium and solely to these cells after enzymic digestion of the tissue. The implications of these results are discussed with respect to potential role of IGF-BP in the action of IGF upon the IGF-1 receptor-bearing populations, including lymphocytes and trophoblast cells, D in the decidua.     

Empirically derived pain-patient MMPI subgroups: Prediction of treatment outcome

Fifty-seven male chronic pain patients admitted to an inpatient multimodal pain treatment program at a Midwestern Veterans Administration hospital completed the MMPI, Profile of Mood States (POMS), Tennessee Self-Concept Scale (TSCS), Rathus Assertiveness Schedule (RAS), activity diaries, and an extensive pain questionnaire. All patients were assessed both before and after treatment, and most also were assessed 2–5 months prior to treatment. No significant changes occurred during the baseline period, but significant improvements were evident at posttreatment on most variables: MMPI, POMS, TSCS, RAS, pain severity, sexual functioning, and activity diaries. MMPI subgroup membership, based on a hierarchical cluster analysis in a larger sample, was not predictive of differential treatment outcome. Possible reasons for comparable treatment gains among these subgroups, which previously have been shown to differ on many psychological and behavioral factors, are discussed.This paper originally was presented at the Fourth World Congress on Pain, Seattle, Washington, September, 1984.     

血管内皮生长因子反义基因转染对高转移人巨细胞肺癌细胞系生 …

目的 探讨反义血管内皮生长因子（ＶＥＧＦ）基因转染在抑制恶性肿瘤生长和转移的抗肿瘤血管基因治疗中的意义。方法 利用基因重组技术构建正义和反义ＶＥＧＦ１２１ ｃＤＮＡ真核表达载体,用脂质体法转染高转移性人巨细胞肺癌细胞（ＰＧ）,经Ｎｏｒｔｈｅｍ杂交和Ｗｅｓｔｅｒｎ印迹免疫化学检测ＶＥＧＦ ｍＲＮＡ和蛋白质的表达水平,并对转染前后细胞进行体外生长和裸鼠体内生长转移等多项生物学行为实验,结果 转染反义转     

Effect of sex and the phase of the estrous cycle on intensity of injury and repair processes in the liver of rats with acute carbon tetrachloride poisoning

The pattern of injury and repair in the liver of Wistar rats depending on sex, the phase of the estrous cycle, and also under the conditions of deficiency of female sex hormones and after injection of -estradiol into ovariectomized rats was studied by morphometric, histo chemical, and electron-microscopic methods. Structural disturbances caused by CCl₄ were found to be increased and reparative reactions inhibited in the liver of females both during the period of a natural increase in the blood estrogen concentration and under the influence of exogenous estradiol, and ovariectomy also had a protective effect. In males, structural changes in the liver were more marked than in females with a low blood estrogen level and differend only a little from those in females during the period of increased secretion of sex steroids.Central Research Laboratory, Novosibirsk Medical Institute. (Presented by Academician of the Academy of Medical Sciences of the USSR V. P. Kaznacheev.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 85, No. 4, pp. 460–464, April, 1978.     

c-erbB-2、VEGF和组织蛋白酶D在胃癌中的表达及其相关性

目的：探讨癌基因c-erbB-2、血管内皮生长因子（VEGF）和组织蛋白酶D（Cath-D)在胃癌中的表达及其与胃癌生物学行为的关系。方法：采用免疫组织化学（S－P）法，检测了102例胃癌手术标本中c-erbB-2、VEGF及Cath-D的表达，同时观察了网状纤维分布与c-erbB-2、Cath-D之间的关系，并将检测结果与跟踪随访资料进行了综合分析。结果：102例胃癌组织中c-erbB-2表达阳性率为38.24%（39／102），与胃癌浸润深度（P＜0.05）、淋巴结转移（P＜0.05）密切相关；VEGF表达阳性率为50％（51／102），与胃癌浸润深度（P＜0.01）、生长方式（P＜0.01）、淋巴结转移（P＜0.01）密切相关；Cath-D表达阳性率为81.37%(83/102)，与胃癌浸润深度（P＜0.05）、生长方式（P＜0.05）、淋巴结转移（P＜0.05）及脉管内有无癌栓（P＜0.05）有关。对生存期分析显示：Cath-D、VEGF、c-erbB-2阳性患者预后差，5年生存率低于阴性表达患者。结论：c-erbB-2、VEGF及Cath-D与胃癌的生长、浸润、转移、预后有密切关系，可作为判断胃癌生物学行为和预后的重要指标。     

集训新兵躯体化症状特点及其相关因素分析

目的：探讨集训新兵躯体化症状特点及其相关因素。方法：用成人EPQ和症状自评量表（SCL-90）在集训过程中对1432名新兵进行了躯体化症状和个性评定。结果：集训第一个月末新兵躯体化症状的检出率最高（12.25％），集训第二个月末为9.81％，集训前为8.66％。逐步回归分析表明，影响躯体化症状的主要因素依次为情绪不稳、有过受伤史、缺少体育锻炼、非主动要求参军等。结论：集训期间躯体化症状呈非线性下降趋势，集训前躯体化症状受多种因素影响。