Pre-differentiated human committed T-lymphoid progenitors promote peripheral T-cell re-constitution after stem cell transplantation in immunodeficient mice

T-cell re-constitution after allogeneic stem cell transplantation (alloSCT) is often dampened by the slow differentiation of human peripheral blood CD34(+) (huCD34(+) ) hematopoietic stem cells (HSCs) into mature T cells. This process may be accelerated by the co-transfer of in vitro-pre-differentiated committed T/NK-lymphoid progenitors (CTLPs). Here, we analysed the developmental potential of huCD34(+) HSCs compared with CTLPs from a third-party donor in a murine NOD-scid IL2Rγ(null) model of humanised chimeric haematopoiesis. CTLPs (CD34(+) lin(-) CD45RA(+) CD7(+) ) could be generated in vitro within 10 days upon co-culture of huCD34(+) or cord blood CD34(+) (CB-CD34) HSCs on murine OP9/N-DLL-1 stroma cells but not in a novel 3-D cell-culture matrix with DLL-1(low) human stroma cells. In both in vitro systems, huCD34(+) and CB-CD34(+) HSCs did not give rise to mature T cells. Upon transfer into 6-wk-old immune-deficient mice, CTLPs alone did not engraft. However, transplantation of CTLPs together with huCD34(+) HSCs resulted in rapid T-cell engraftment in spleen, bone marrow and thymus at day 28. Strikingly, at this early time point mature T cells originated exclusively from CTLPs, whereas descendants of huCD34(+) HSCs still expressed a T-cell-precursor phenotype (CD7(+) CD5(+) CD1a(+/-) ). This strategy to enhance early T-cell re-constitution with ex vivo-pre-differentiated T-lymphoid progenitors could bridge the gap until full T-cell recovery in severely immunocompromised patients after allogeneic stem cell transplantation.     

Increased killer cell activity in insulin dependent (Type 1) diabetes mellitus

Summary Antibody dependent cell mediated cytotoxicity in relation to the levels of circulating killer cells was investigated in 16 newly diagnosed classical insulin dependent (Type 1) diabetics, 11 islet cell antibody positive non diabetic children with at least one HLA haplotype in common with their diabetic sibling, and in 15 normal controls. Antibody dependent cell mediated cytotoxicity was evaluated using, as target, ⁵¹Cr labelled human 0⁺ erythrocytes sensitised with an anti-CD antiserum. Killer cells were measured by the low affinity E-rosetting cell technique. Increased killer cell levels (>normal mean + 2SD) were accompanied by a significant enhancement in antibody dependent cell mediated cytotoxicity both in newly diagnosed diabetics (p < 0.05) and in unaffected siblings (p < 0.01). These preliminary results indicate that raised antibody dependent cell mediated cytotoxicity is a feature of insulin dependent diabetes at diagnosis and suggest that active B cell damage might be occurring some time before the onset of clinical symptoms.     

肺结节的中医诊治思路综述

肺结节可归属中医学"肺积"的范畴,主要病位在肺,涉及肝、脾、肾,以肺脾肾三脏气血阴阳虚弱为本,由外来六淫邪气或雾霾毒邪引发,以痰瘀互结为主要病理表现,虚实夹杂是其主要病机特点,现代医家认为肺结节的治疗宜扶正祛邪并举,提倡综合体质、因人制宜的辨证方法.攻补兼施应贯穿肺结节的治疗始终,根据虚实偏重合理遣方,对于无证可辨的患...     

武警某部新兵中医体质辨识与分析

 目的 按中医学理论了解武警某部新兵中医体质状况及其影响因素。方法 采用《中医体质量表》对武警某部2016年9月入伍的506名新兵进行问卷调查,依据《中医体质分类与判定》标准辨识中医体质,比较不同体质类型在新兵中的构成差异。结果 506名新兵中,单一体质352名(69.57%),兼夹体质154名(30.43%)。单一体质中,平和质139名(39.49%),偏颇质213名(60.51%)。单一偏颇质中气虚质、阳虚质、痰湿质比例较高,分别占12.50%、10.51%、10.23%。不同入伍地、户籍性质、文化程度、体质指数的新兵体质类型分布情况不同,差异有统计学意义(P<0.05)。结论 武警某部新兵中医体质状况良好。新兵中医体质类型与入伍地、文化程度、户籍性质、体质指数等因素有关,可为运用中医“治未病”预防思想引导新兵顺利度过第一适应期提供参考数据。
     

气虚体质的调养方法

目的研究气虚体质的调养方法。方法根据祖国医学对气虚体质调养的认识，结合近年来气虚体质的研究成果进行研究总结，探讨气虚体质调养的必要性、可行性和具体方法。结果气虚体质是形成许多疾病的内在基础，有必要早期干预。气虚体质调养的方法包括膳食调养法、中药调养法、针灸调养法、情志调养法等。结论应用上述调养法调养气虚体质切实可行。     

气象体质因素与脑梗塞冠心病2型糖尿病的相关性研究

论述气象因素与体质因素与脑梗塞、冠心病、2型糖尿病的相关性,认为气象、体质因素与脑梗塞、冠心病、2型糖尿病的发生、发展、转归及预后密切相关,临床上必须结合气象与体质因素进行辨证施治与预防。     

中国汉族人群Ⅱ型糖尿病家系1号染色体易感基因的精细定位

目的 通过增加微卫星位点密度、扩大样本量、对以前定位到1号染色体上的中国北方汉族人群2型糖尿病相关易感基因研究结果进行验证。方法 运用基因组扫描的方法,经多重PCR、电泳、GeneScan及Genotyper程序分析获取位点信息,最后经参数及非参数连锁分析,得到相关的P值、NPL值（Z值）。结果 共扫描了5个区域34个位点,检出约12000个基因型。经GENEHUNTER 2.0软件分析,其中的8个位点可能与糖尿病相连锁（P＜0.05,NPL值最大为2.17),它们分布于3个区域,尤其是第1个区域（1 p36.3-1p36.23),其每一个位点都显示与糖尿病相连锁,前后分布于1个长达16.9cM的范围内。另外2个区域未检出阳性结果。结论 证实了全基因组扫描所获得的结果。尤其是,在所研究的1P末端区域,所有研究位点都显示与疾病连锁,这些位点分布在一个很宽的范围内,提示该区域可能蛰伏着多个糖尿病易感基因。     

Alveolar permeability to protein in rats differentially susceptible to ozone

Sprague-Dawley rats susceptible (DS) to NaCl-induced hypertension suffer higher mortality when exposed daily to 2.0 ppm ozone than do hypertension-resistant (DR) rats, independent of salt in the diet or systemic blood pressure. To investigate one possible contribution to this differential sensitivity to ozone, alveolar permeabilities to serum albumin were measured both in ozone-exposed and in control DS and DR rats. Female rats aged 5-7 weeks maintained on a low-salt (0.4% NaCl) diet were injected intravenously with 125I-bovine serum albumin and were then exposed to either 2.0 ppm ozone or air for 5 h. After pentobarbital anesthesia, the rats were exsanguinated and their lungs were lavaged in situ with saline. Lavage fluids and blood samples were measured for radioactivity using a NaI-well gamma counter. The results indicated that while DS and DR control rats have similar pulmonary permeabilities to 125I-albumin, the lungs of the ozone-exposed DS animals were 63% (p less than 0.02) more permeable than those of DR rats exposed to ozone. Sloughing of epithelial tissue, mucous formation and an accumulation of macrophages in the end-airways were more pronounced among ozone-exposed DS animals than in DR-ozone-exposed rats. This increased damage among DS rats correlated well with the increased protein permeability levels. In similar studies, Sprague-Dawley (D) rats were more variable in their response to ozone than either inbred strain. However, the results appeared generally more like those of the DS animals, suggesting that the trait selected by inbreeding may have been resistance rather than sensitivity to ozone-induced lung injury.     

"三阴三阳体质学说"与糖尿病防治思路

糖尿病是一种临床常见病和多发病，中医药防治糖尿病意义深远。通过阐述“三阴三阳体质学说”中体质类型与糖尿病发病及证候和并发症演变的重要关系，试探求为中医药防治糖尿病研究的新思路。有助于达到更富有针对性的个体化预防及诊疗糖尿病人群之根本目的。     

体质医学概说

简述了体质医学在西方发展情况以及受重视的原因,并且说明了它在预防疾病和治疗疾病中的重要作用。