Organophosphorus pesticide exposure decreases sperm quality: association between sperm parameters and urinary pesticide levels

Several studies have suggested that human semen quality has declined over the past decades and some of them have associated it with occupational exposure to pesticides. However, most of these studies have not been associated with a reliable exposure level and have been designed mostly as cross-sectional studies. The present work evaluates, in a longitudinal follow-up study, the effect of organophosphate pesticides (OP) at three occupational exposure levels on semen quality. In addition, the study examined the association between OP urinary levels and sperm parameters in exposed and unexposed workers. A total of 139 semen samples from 52 volunteers were assessed. Urinary OP levels were measured by gas-liquid chromatography.The results revealed that the poorest semen quality was found among the subjects with the highest OP exposure and the highest urinary OP levels. Seasonal variations in sperm concentration and sperm count were registered.The results showed a significant decrease in total sperm count among subjects with the highest exposure to OP. Further studies assessing the effects of OP on male reproductive health should be controlled by the variability in human sperm parameters, sperm seasonality, spermatogenesis time and the changing OP exposure level in men highly exposed to OP.     

辅助生殖中受精的方式与自然流产关系的探讨

目的 探讨在辅助生殖中不同的受精方式与自然流产的关系.方法 行体外受精-胚胎移植(IVF-ET)及单精子卵细胞浆内显微注射-胚胎移植(ICSI-ET)助孕的481个周期进行回顾性分析,按不同的受精方式分为IVF-ET组和ICSI-ET组,比较两组的一般临床资料、早期流产和晚期流产发生率.结果 ICSI-ET组的不孕年限、用促性腺激素(Gn)总量(支数)均低于IVF-ET组(P均＜0.05),两组的的年龄、Gn启动剂量、用Gn天数、移植胚胎数、ET优质胚胎数目差异均无统计学意义(P均>0.05),ET后妊娠总的自然流产率17.54%,其中受精方式为IVF妊娠者早期流产率和晚期流产率分别为12.93%和4.08%,受精方式为单精子卵细胞浆内显微注射(ICSI)者早期流产率和晚期流产率为20.83%和0.00%,两组早期流产率和晚期流产率差异无统计学意义(P>0.05).结论 行ART助孕妊娠者早期流产率与自然受孕妊娠者的早期流产率10%～15%相近,行ICSI妊娠者早期自然流产率和晚期流产率与行IVF妊娠者相比无差别.