以临床技术流程功能定位需求为基础的生理监测仪功能配置

病人生理监测仪是临床医学的主要仪器,由于涉及技术流程的功能定位不同,功能需求也就不同,其功能配置也就有所不同。从生理监测仪相关应用领域的分解技术功能定位出发,讨论装备管理的功能配置问题。     

论河北省卫生系统办公自动化管理系统

系统采用基于TCP/IP协议的国际互联网为基础,采用B/S结构,客户端采用IE浏览器,服务器端采用WINDOW2000的IIS作为服务器。其主要功能:工作督办、工作信息、电子邮件、收文、发文、图书管理、档案管理、车辆管理、日程管理、待办事宜、电子论坛、考勤管理等。     

Evaluation of stroke rehabilitation by randomized controlled studies: a review

In all, 22 reports of 20 randomized, controlled rehabilitation studies were evaluated. In 18 of these, the design of the trial was parallel, with a cross-over format being employed in the remaining 2 instances. Seven studies related to intensive rehabilitation during the early post-acute period. In six others, specific techniques--sometimes associated with traditional physiotherapy procedures--were compared: biofeedback, perceptual retraining, and amphetamine treatment. Eight experiments evaluated speech therapy in aphasia. Frequently, methodological considerations limited the interpretation of the results. The review showed that: 1) as regards activities of daily life and motor function, differences as between rehabilitation in stroke units on the one hand and non-rehabilitation or rehabilitation in medical wards on the other, were detected in relatively few quality studies and remained particularly inconclusive insofar as life in the home environment was concerned; 2) rehabilitation for aphasia and perceptual dysfunction proved effective for at least several months after acute stroke; 3) in general, examination of the reports cited revealed no differences in the effects of biofeedback and perceptual retraining vs conventional physiotherapy; 4) rehabilitation, whether administered by specialists or amateur caregivers purpose-trained by specialists, proved equally effective for aphasia. These conclusions constitute a valuable basis for the development and evaluation of modern rehabilitation programs for stroke patients.     

树型金银花无公害优质加工技术的研究

目的探讨树型金银花无公害优质加工技术。方法采用树型金银花晾晒和烘烤以及"烤房3段变温烘烤技术"。结果烘干产品比晒干产品具有明显的质量和价格优势,价格高57.1%。烤房3段变温技术第1段烘干温度控制在35～40℃,烘8～9h;第2段将温度提高到50～55℃左右,烘烤6～8h;第3段温度升到60～70℃,烘烤5～6h左右;烘干总时间不超过24h。结论无公害优质加工技术利于规模化生产,获得无公害的绿色产品,增加金银花附加值。     

反义核酸技术及应用

反义核酸技术是用人工合成的或生物合成的特定互补的DNA或RNA片段阻断从基因到蛋白的信息流 ,以达到抑制或阻断基因表达的生物新技术。利用核酸分子杂交原理将反义寡核苷酸 (antisenseoligonucleotide,ASON)作为外源性基因表达抑制物结合到靶mRNA序列上阻止其翻译成蛋白[1 ] 。具有杂交特异性和选择性调节基因的表达的特点[2 ] ,这一技术不仅已成为常用的实验室研究方法 ,还广泛的应用于研究病毒、肿瘤、寄生虫和遗传性疾病的预防和治疗 ,具有重要的应用前景。对于人类基因的研究 ,人们采用分子克隆、分子杂交、PCR技术等一系列研究…     

Flow karyotypes and chromosomal DNA contents of genus Triticum species and rye (Secale cereale)

The flow cytometry and chromosome imaging method were jointly used for analyzing genome content and chromosomal DNA content of hexaploid wheat (AABBDD), hexaploid triticale (AABBRR), tetraploid wheat (AABB), and AA, BB, DD genome donors and RR genome rye. Their genome sizes were 34.4 pg, 40.9 pg, 26.2 pg, 12.1 pg, 13.7 pg, 10.5 pg, and 16.9 pg, respectively. The 2C nuclear DNA content of BB genome donor with 13.7 pg was the highest value among the other genome donors, AA or DD. The genome content of tetraploid wheat, unlike hexaploid wheat or hexaploid triticale, was larger than the sum of the genomes of AA and BB genome donors. The DNA content of each chromosome ranged from 1.22 pg in DD genome donor to 2.61 pg in rye. Each chromosome peak was divided into three to four groups. Only one chromosome was included in the highest chromosomal DNA peak in hexaploid wheat, tetraploid wheat, DD genome donor and rye but two chromosomes in AA, BB genome donors, and hexaploid triticale. Correlation between 2C nuclear DNA content and chromosome density volume was the highest value compared with the other chromosomal parameters of chromosome area, or chromosome length.     

消渴降糖胶囊制备工艺的研究

目的 研究消渴降糖胶囊制备工艺.方法 采用高效液相色谱仪,以甲醇-0.4%磷酸溶液(60∶40)为流动相,检测波长为367 nm进行测定,记录色谱图,计算峰面积.结果 提取3 h、提取2 h与提取1 h相比,槲皮素提取率有显著增高(P<0.05),但提取3 h与提取2 h槲皮素的含量无显著性变化,浸膏得率3 h比2 h显著提高(P<0.05);加醇量4.0倍与3.0倍相比较,浸膏得率显著降低(P<0.05),槲皮素含量相差不大.结论 在制备消渴降糖胶囊工艺中,应减少能源消耗,降低无效成分的提取率,减少浸膏量.在尽量减少杂质,保留有效成分,降低服用量的综合考虑下,采用4.0倍量乙醇进行醇沉有效成分损失少,浸膏得率低,符合工业化要求.     

流行性乙型脑炎病毒结构蛋白编码基因的重组构建

目的:建立流行性乙型脑炎病毒(JEV)C、prME,E等结构蛋白编码基因克隆.方法:病毒感染C6/36细胞,RT-PCR法依次获取该病毒结构蛋白基因并进行DNA测序分析,PCR克隆法将PCR产物分别构建于真核表达载体pcDNA 3.1的BamHI与EcoRI酶切位点并依次命名为pJC、pJME与pJE,通过DNA测序、电泳以及限制性内切酶分析加以鉴定.结果:JEV C、prME与E基因片段分别为380、2001以及1 500bp,各基因测序结果与发表的JEV JaGAr-01株相对应序列相一致,从重组质粒释出的插入子分别与各基因大小相符合.结论:pJC、pJME与pJE重组质粒分别含有C、prME与E蛋白编码基因.