Chronic Δ9-Tetrahydrocannabinol in rats: Effect on social interactions,mouse killing,motor activity,consummatory behavior,and body temperature

Behavioral and physiologic effects of daily administration of ⁹-tetrahydrocannabinol (THC) were assessed over a 60-day period, investigating different dose levels (10, 20, 50 mg/kg), routes of administration (i.p., p.o.), drug vehicles [5%–18% propylene glycol(PG)/1% Tween 80-saline; sesame oil], and rat strains (Sprague-Dawley, Wistar, Long-Evans, Fischer). All THC-treated rats showed compulsive motor routines (i.e., forepaw treading, rhythmic jaw movements, prolonged head grooming, and biting of cage wires) after 3–4 weeks. Whether maintained on a restricted feeding regimen or not, three was no evidence for increased aggressive behavior between cagemates in any of the treatment groups. Mouse killing was induced in 70%–75% of all Sprague-Dawley (i.p., PG/Tween 80-saline vehicle) and Fischer rats (p.o., sesame oil vehicle), but not in the other THC-treated groups. Rearing activity was greatly decreased in all THC-treated groups throughout the treatment period. Food and water intake decreased by approximately 40% during the first 2–6 days of THC administration. Growth rate was reduced for the entire treatment period in THC-treated rats. THC decreased rectal temperature on the 1st day, and also on the 2nd day in Fischer rats receiving the drug in sesame oil, but not thereafter. This pattern of effects shows that tolerance develops rapidly to THC's effects on body temperature and consummatory behavior, but not to changes of certain motor activities.     

国内CD44v6与喉鳞癌关系研究的Meta分析

目的对CD44v6与喉鳞癌相关性研究的国内文献进行Meta分析,探讨其病理与临床意义。方法检索中国学术期刊全文数据库、维普及万方数据库,收集1996～2006年国内期刊公开发表的有关喉鳞癌CD44v6表达及其意义的独立病例对照研究论文,在对文献进行异质性检验后,以病例组及对照组比值比（OR值）为效应指标,应用Meta分析软件RevMan4．2．9对各研究报告原始数据进行统计处理,计算合并OR值、95％可信区间及Meta分析森林图。结果最终进入系统评价的文献共有7篇,观察癌患者496例,对照病例99例。Meta分析结果,合并区OR为19．43,95％可信间（CI）为[4．14,91．27],CD44v6表达与喉鳞癌临床分期（p〈0．0001）、分化程度（p=0．0004）、淋巴结转移（P〈0．00001）及5年生存率（P=0．002）均存在明显相关性,而与病变分型（P=0．23）及TNM分期（P=0．12）、患者年龄（P=0．53）及性别（P=0．63）无明显关联。结论相关研究文献的Meta分析显示,CD44v6高表达可能在喉鳞癌的发生、发展、转移及预后中起重要作用。     

增生性玻璃体视网膜病变视网膜前膜CD44v6的表达

目的：观察增生性玻璃体视网膜病变视网膜前膜中CD44v6的表达情况。方法：用免疫组化方法对13例PVR患行玻璃体切割术的剥离的视网膜前膜进行观察。结果：CD44v6阳性表达10例，表达率77％。结论：提示CD44v6在PVR的形成和发展中有一定的作用。     

肿瘤转移相关基因的研究进展

肿瘤的分化、侵袭和转移等一系列病理过程受多种基因的调控,它们在肿瘤细胞浸润和转移机制中或被激活,或被抑制,相互协同或拮抗,最终影响肿瘤细胞的生物学特性。nm23基因抑制肿瘤转移,而CD44v、S100A4基因和TIAM1基因促进肿瘤的扩散。     

骨桥蛋白、CD44v6、膜细胞骨架连接蛋白在胃癌组织中的表达及意义

目的探讨骨桥蛋白（OPN）、CD44v6、膜细胞骨架连接蛋白（Ezrin）在胃癌组织中的表达,并分析OPN、CD44v6、Ezrin与胃癌临床病理特征的关系。方法应用免疫组织化学方法检测OPN、CD44v6、Ezrin在48例胃癌组织中的表达。结果OPN、CD44v6、Ezrin在胃癌组织中阳性表达率分别为68.8%、60.4%、64.6%。伴有淋巴结转移者中OPN表达阳性为21例（87.5%）,在无淋巴结转移者中OPN表达阳性为13例（54.2%）,P＜0.05。伴有淋巴结转移者中CD44v6表达阳性为19例（79.2%）,在无淋巴结转移者中CD44v6表达阳性为12例（50.0%）,＜0.05。伴有淋巴结转移者中Ezrin表达阳性为20例（83.3%）,在无淋巴结转移者中Ezrin表达阳性为11例（45.8%）,P＜0.05。对OPN、CD44v6、Ezrin的表达情况两两之间行Spearman等级相关分析,结果表明在48例胃癌标本中,OPN、CD44v6、Ezrin两两之间的表达均呈正相关。结论OPN、CD44v6、Ezrin在胃癌浸润、转移中起着协同作用,它们可以作为胃癌浸润、转移及评估预后的指标。     

CD44v6在宫颈上皮内瘤样变中的表达及意义

采用免疫组化SP法检测120例宫颈上皮内瘤样变(CIN)中CD44v6蛋白的表达情况,分析CD44v6表达水平与临床病理因素的关系。结果在正常宫颈上皮与不同期别的CIN间CD44v6的表达水平存在显著性差异(P<0.05),CD44v6的表达水平与患者年龄无关。认为CD44v6在CIN的分化、进展过程中起着重要的作用,是早期诊断和评估CIN预后的良好指标。     

子宫内膜整合素αvβ3表达与体外受精-胚胎移植反复种植失败的相关性研究

目的研究整合素αvβ3在人各时期子宫内膜及蜕膜组织的表达,并探讨其与体外受精-胚胎移植（IVF-ET）反复种植失败的相关性。方法以40例IVF-ET治疗反复种植失败的患者为研究对象,以40例因男性因素行卵胞浆内单精子注射（ICSI）治疗的患者及5例正常孕早期患者作为对照,获取患者各个时期的子宫内膜及蜕膜组织,采用免疫组织化学法检测整合素αvβ3蛋白的定位和表达,并对所获得的结果应用Image-ProPlus6．0图像分析软件测定阳性反应强度。结果整合素αvβ3蛋白定位于子宫内膜腺上皮细胞和腔上皮细胞的细胞浆;增殖早、中、晚期及分泌早期内膜上均无表达,分泌中期、晚期内膜明显表达,蜕膜中表达最强（P〈0．05）。反复种植失败患者子宫内膜整合素αvβ3的表达同样存在周期性变化,但其在分泌中、晚期的表达要明显弱于对照组,差异有统计学意义（P〈0．05）。结论整合素αvβ3在正常子宫内膜组织中的表达呈现明显周期依赖性,与胚胎种植密切相关;而其在IVF—ET反复种植失败患者种植窗期子宫内膜组织中的表达明显减弱,可能是导致胚胎种植失败的原因之一。     

CD44v9和MMP-9在结直肠癌组织中表达的临床意义

目的研究CD44v9和基质金属蛋白酶-9(MMP-9)在结直肠癌和癌旁组织中表达的临床意义。方法采用RT-PCR方法分别检测46例结直肠癌及癌旁组织CD44v9及MMP-9的阳性表达情况。结果46例结直肠癌组织中CD44v9和MMP-9的阳性表达率分别是58.7%和82.6%,明显高于癌旁组织(58.7%和47.8%),两者比较差异均有统计学意义(P<0.001)。CD44v9和MMP-9在结直肠癌组织的阳性表达率与肿瘤的大小、分化高低、浸润深度、临床分期及淋巴结转移相关,而且两者在结直肠癌中的表达呈正相关。结论CD44v9和MMP-9与结直肠癌侵袭和转移性有关,可作为预测肿瘤转移潜能的指标。     

T cells in patients with chronic Tγ lymphocytosis: Morphology,cytochemistry, ultrastructure and immunological characteristics

Summary We have studied the morphology and cytochemistry in relation to the immunological phenotyping and functional properties of T cells from eight patients with chronic T lymphocytosis. At the light microscopic level the morphology of the patients' lymphocytes was similar to that described for large granular lymphocytes. Ultrastructurally, a division into two groups could be made on differences in the amount of cytoplasm and the location and the more irregular form of the nuclei. The lymphocytes of one group (five patients) had in common the phenotype Fc+, T3+, T4–, T8+, Ia–, M1– and demonstrated (with the exception of one patient) the same functions: presence of K-cell activity, absence of NK, helper and suppressor cell activities. In the other group (three patients), the lymphocytes of one patient showed the same phenotype and functions as those indicated above. The other two patients both lacked the T8 antigen on their lymphocytes but were different with regard to other surface markers. In addition, their cells were functionally identical: both demonstrated NK- and K-cell activity. Thus in this group of eight patients with chronic T lymphocytosis, the immunological and functional subdivision paralleled in part a morphological division at the ultrastructural level.