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21.
Jhoji Tanabe Hirohiko Nakazato Hisayoshi Tanabe Hidetoshi Hanasaki Kazuo Kawasaki Daizo Yonemura 《Documenta ophthalmologica. Advances in ophthalmology》1986,63(2):165-171
The spectral response curve (amplitude versus wavelength) of the R2 of the early receptor potential (ERP) was studied in normal, protan, and deutan subjects. The R2 amplitude peaked at 520nm in most normal subjects. The R2 at long wavelengths was smaller than normal in protans and larger than normal in deutans when the maximum amplitudes were normalized to 100% at the peak. The ratio of the R2 amplitude at 460 nm to that at 600 nm clearly differed between protans and deutans. The ERP and the rapid off-response, which is mainly due to the cessation of the late receptor potential, were recorded in the same subjects. The ratio of the sensitivity of the rapid off-response at 500 nm to that at 600 nm was correlated with the ratio of the R2 amplitude at 460 nm to that at 600nm (correlation coefficient, 0.823, p < 0.001). This study, in conjunction with our previous study, indicates that the abnormality is in the outer segments of the cones in protans and deutans. 相似文献
22.
目的观察快速心房起搏对家兔心房L型钙通道亚单位和Kv4.3钾通道基因表达的影响。方法新西兰大耳白家兔36只,随机分成6组,经右颈外静脉穿刺置入电极于右房,分别给予0、3、6、12、24或48h快速心房起搏,停止起搏后取右房组织,应用半定量反转录聚合酶链式反应测定各时相点L型钙通道α1c,β1,α2亚单位,钾通道Kv4.3mRNA的表达水平。结果L型钙通道α1c、β1亚单位在快速起搏6h后表达水平下调,并随着起搏时间的延长进一步下调。α2亚单位的mRNA表达在各时相点无显著差异。Kv4.3的mRNA的表达在快速起搏的24h和48h下降分别达55.50%(P<0.01)和59.12%(P<0.01)。48h后下降达到一个平台期。结论快速心房起搏可导致L型钙通道亚单位和Kv4.3钾通道mRNA表达下调。 相似文献
23.
目的:通过对HIV抗体快速检测试剂的临床评估,为HIV检测试剂的临床应用提供参考.方法:对快速试剂的使用性能进行比较,并采用美国输血协会(AABB)血清盘、临床样品血清盘、特性血清盘(阳性样品盘)、稀释系列血清盘评估快速试剂的敏感性和特异性.结果:三种快速试剂在检测AABB血清盘时,敏感性分别是86.4%、86.4%、100%,特异性皆为100%;三种快速试剂在检测临床样品血清盘时,敏感性分别是88.6%、91.4%、97.1%,特异性皆为100%.快速试剂具有很高的阳性预期值,对于低危人群(感染率很低人群)也具有很高的阴性预期值.快速试剂检测弱阳性的样品(酶联试剂s/co比值小于6~8的样品)存在漏检.快速试剂与ELISA参考试剂在分析灵敏度方面相差3个以上倍比稀释度.结论:快速试剂具有较好的使用特性,非常适用于样本量较少的实验室及对低危人群的HIV筛查,在对高危人群筛查时,可能有弱阳性样品漏检,同时快速试剂在分析灵敏度方面有待提高. 相似文献
24.
R W Tennant 《Environmental and molecular mutagenesis》1991,18(4):322-323
The development of transgenic mouse lines with target genes that are suitable for tissue-specific mutagenesis studies is an important contribution to the field of environmental mutagenesis. These models can accommodate many of the questions relating to metabolism, distribution and relative potency of mutagens as well as providing a more comprehensive system for the identification of mutagens. However, the lesions that the field has learned about methods, development and validation from the use of in vitro systems must be applied to the development and validation of transgenic models. 相似文献
25.
26.
It has recently been shown that it is possible to discriminate accurately among myoelectric signals underlying different muscle
contraction types, specifically elbow flexion and extension and forearm pronation and supination. It was reported that once
a number of distinctive features had been extracted from the myoelectric signals, a neural network could be trained to distinguish
the contraction types with an impressively high accuracy. In the present paper, we show that a technique known as parallel
cascade identification can be used to construct classifiers that can also accurately, differentiate the contraction types.
The use of parallel cascades has the benefit of dispensing with the need for feature extraction, so that raw myoelectric signal
data can be used directly. In addition, very little data are required to train the parallel cascades to distinguish accurately
novel incoming myoelectric signals. Results of using parallel cascades to distinguish foream pronation, supination, and elbow
flexion are presented. 相似文献
27.
对定坤丹(蜜丸)进行了显微鉴定研究,将29种组成药物全部检出,对各组成药物的显微鉴别特征作了简明描述,并附显微特征图。 相似文献
28.
While much research has been devoted to the implementation and application of Volterra and Wiener functional series expansions
in the identification and characterization of biological systems, little effort has been focused on the fundamental problem
of interpreting the resulting kernels. This paper describes the application of the series to the components of a known model
of the human pupil control system. As more complicated elements are put together, insight into kernel interpretation is built
up incrementally until the total system is identified. Practical limitations and methods are also discussed. 相似文献
29.
以方格星虫(Sipunculus nudus)体壁和血液为试验材料,经胰蛋白酶水解,Sevage法去除蛋白质,乙醇沉淀得到粗多糖,透析并冷冻干燥后分别得到方格星虫体壁多糖(SWP)和血液多糖(SBP)。经凝胶渗透色谱分析,SWP和SBP均为单一组分,相对分子质量分别为284528和198212。红外光谱分析表明,两种多耱结构相似,不含硫酸基团,均含有葡萄耱、半乳糖、木糖和阿拉伯糖。SBP含有乙酰氨基,而SWP不含乙酰氨基,它们是以α-糖苷键或β-糖苷键连接的呋喃多糖。 相似文献
30.
目的 克隆出视蛋白基因启动子。方法 以小鼠全基因组为模板,用PCR法克隆出目的大小的片断。然后连接到T-载体上作酶切鉴定,最后测序。结果 酶切结果与预期相符,测序结果与公布序列完全一致。结论 视蛋白基因启动子克隆成功。 相似文献