甘草查尔酮抗脂质过氧化及自由基的实验研究

应用比色法测定脂质过氧化物研究甘草查尔酮（Ｌｉｃｏｃｈａｌｃｏｎｅ，ＬＣ）的抗脂质过氧化作用。并用电子自旋捕集技术检测其对超氧阴离子自由基及羟自由基（ＯＨ－）的清除作用。实验表明，ＬＣ５０～２００ｍｇ／Ｌ时，可明显抑制家兔脑组织在温育下引起的丙二醛升高（Ｐ＜０．０１），ＬＣ２００ｍｇ／Ｌ对Ｆｅｎ－ｔｏｎ反应生成的ＯＨ－清除率达６８．３％，而对黄嘌呤／黄嘌呤氧化酶系统生成的清除率仅为１１．４％。结果表明，ＬＣ具有明显的抗脂质过氧化作用，其作用机制可能是对ＯＨ－的直接清除作用所致。     

The radix entomolaris in mandibular first molars: an endodontic challenge

AIM: To present cases of mandibular first molars with an additional distolingual root (radix entomolaris, RE) and to survey the literature on the incidence of this anatomical feature. SUMMARY: A major anatomical variant of the two-rooted mandibular first molar is a tooth with an additional distolingual and third root: the RE. The prevalence of these three-rooted mandibular first molars appears to be less than 3% in African populations, not to exceed 4.2% in Caucasians, to be less than 5% in Eurasian and Asian populations, and to be higher than 5% (even up to 40%) in populations with Mongolian traits. A total of 18 cases (12 root filled and six extracted mandibular first molars) with an RE were collected during the years 2000-2003 in patients of Caucasian origin. As far as the access was concerned, entering the root canal in the RE required a modification of the opening in a distolingual direction resulting in a trapezoidal opening cavity. None of the orifices was located midway between the mesial and distal root component. Based on the anatomy of the extracted samples and the bending of ISO 10 files after scouting of the root canal in the RE, three types of curvature were detected: (I) straight or no curvature (two cases); (II) coronal third curved and straight continuation to the apex (five cases); and (III) curvature in the coronal third and buccal curvature from the middle third or apical third of the root (11 cases). KEY LEARNING POINTS: Clinicians should be aware of this unusual root morphology in mandibular first molars in Caucasian people. Radiographs exposed at two different horizontal angles are needed to identify this additional root. The access cavity must be modified in a distolingual direction in order to visualize and treat the RE, this results in a trapezoidal access cavity.     

野生与栽培明党参抗疲劳和耐缺氧作用比较

实验表明，明党参根提取物具有非常明显的抗疲劳作用（Ｐ＜０．０１）和较为明显的耐缺氧作用（Ｐ＜０．０５）。其中抗疲劳作用优于人参。野生品明党参的作用优于栽培品的作用。提示明党参是具有开发潜力的保健中药。     

Protective effect of Astragali radix extract on interleukin 1beta-induced in fl ammation in human amnion

The aim of this study was to investigate the effect of Astragali radix extract on interleukin (IL)-6 and tumour necrosis factor (TNF)-alpha production and prostaglandin E(2) (PGE(2)) and leukotriene C(4) (LTC(4)) release from IL-1beta-stimulated human amnion. Primary monolayer cultures of amnion cells were established from women undergoing elective caesarean section before the onset of labour. Production of both IL-6 and TNF-alpha was stimulated in a concentration-dependent manner by proinflammatory cytokine IL-1beta (0.01-10 ng/mL). Astragalus extract inhibited IL-6 production by approximately 75% from cells under IL-1beta-stimulated conditions. Treatment of amnion cells with IL-1beta (0.01-10 ng/mL) resulted in a significant increase in PGE(2) release. Incubation of the cells with the extract for 24 h significantly inhibited IL-1beta-induced PGE(2) production. A concentration-dependent increase in LTC(4) production by amnion cells occurred in response to IL-1beta. Astragalus extract blocked the effect of IL-1beta in LTC(4) production in human amnion. These results indicate that Astragali radix has a broad antiinflammatory effect in human amnion and may be considered a promising agent to protect preterm labour.     

丹参外用促进创面愈合的实验与临床研究

目的：探索加快创面愈合的有效方法。方法：将丹参注射液外用于创面进行实验研究和临床应用。结果：外用丹参组比对照组创面愈合天数少２．２天，肉芽生长快，毛细血管和成纤维细胞数量多，炎细胞少，创缘上皮组织向中心生长快。临床应用１５例病人效果好，无副作用。结论：丹参外用确实能促进创面愈合，是一种较理想的新方法     

黄芪中有效成分环黄芪醇皂甙和黄芪甲甙的含量测定

本文以双波长薄层扫描λｓ＝５００ｎｍ，λＲ＝７００ｎｍ测定环黄芪醇皂甙和黄芪甲甙的含量。     

丹参对肝硬化门脉高压大鼠缺血再灌注胃粘膜损伤防治作用的研究

制成肝硬化门脉高压（ＰＨＴ）大鼠胃粘膜缺血再灌注模型，用电子自旋共振技术测定缺血再灌注前后及丹参治疗后胃粘膜中氧自由基（ＯＦＲ）以及脂质过氧化物（ＬＰＯ）和超氧化物的歧化酶（ＳＯＤ）的变化，同期观察其光镜、电镜病理改变，探讨丹参对胃粘膜损伤的保护作用。结果表明：ＰＨＴ大鼠胃粘膜在缺血再灌注过程中有大量ＯＦＲ产生，粘膜损伤严重程度与ＯＦＲ、ＬＰＯ及ＳＯＤ密切相关；ＰＨＴ胃粘膜更易受缺血再灌注损伤；早期使用丹参可显著降低胃粘膜中ＯＦＲ及ＬＰＯ含量，提高ＳＯＤ活性，减轻胃粘膜的损伤，起到保护胃粘膜的作用     

当归等对博莱霉素致鼠肺损伤和脂质过氧化物生成的影响

利用博莱霉素致大鼠肺损伤模型,观察了当归、丹参及消炎痛对博莱霉素致肺损伤的病理改变、血清脂质过氧化物含量和肺湿重/体重的变化。结果发现,当归和丹参可明显减轻肺泡炎的严重程度,降低肺湿重/体重(P<0.01),并可抑制脂质过氧化物的产生(P<0.01),消炎痛则无上述作用。表明当归和丹参可阻抑博莱霉素引起的急性肺损伤,作用机理可能是两药对自由基的清除作用。