大鼠脑纹状体微透析法研究天王补心方剂中桔梗的应用与抑制性神经递质变化的相关性

目的：研究天王补心丸对大鼠脑内氨基酸类神经递质的影响，来探讨该药的安神作用机制。方法：利用微透析技术从给药大鼠脑纹状体取样，利用邻苯二甲醛(OPA)柱前衍生反相高效液相色谱法结合荧光检测器测定标准品和样品中抑制性氨基酸(甘氨酸、γ-氨基丁酸)的含量并进行统计分析。结果：全方组、桔梗+酸枣仁组给药后大鼠纹状体区细胞外液中兴奋性氨基酸含量变化不明显，而抑制性氨基酸GABA和Gly含量均有所增加。结论：本方法具有快速、准确、灵敏度高、稳定性好等优点，适用于脑组织中微量氨基酸类神经递质的测定。桔梗对大鼠脑中抑制性神经递质有明显的影响。     

黄芪胶囊对化学性肝损伤大鼠的治疗作用

目的通过黄芪胶囊对CCl4大鼠慢性肝损伤的治疗试验,评价黄芪胶囊保肝降酶的作用。方法大鼠sc 2 mL.kg-125%CCl4,每周2次,连续8周,制作大鼠慢性肝损伤模型。自给CCl4第5次后,开始ig黄芪胶囊,qd,连续5周,观察黄芪胶囊对CCl4造成大鼠慢性肝损伤的治疗作用。结果黄芪胶囊显著降低大鼠血清丙氨酸氨基转移酶、天门冬氨酸氨基转移酶,降低肝干重/湿重比值,降低肝系数,减轻肝组织病理损伤的程度,并增加肝细胞中肝糖原的含量。结论黄芪胶囊通过降低转氨酶,升高肝糖原水平,减轻肝脏损伤程度从而起到对CCl4引起大鼠肝损伤的保护作用。     

复方苦参联合热疗治疗消化道肿瘤的疗效分析

目的：观察复方苦参联合体外高频热疗治疗晚期消化道肿瘤患者的临床疗效。方法：治疗组：采用体外高频热疗机进行局部肿瘤部位照射,隔日1次,i次60—120min,10次为一疗程,复方苦参注射液50ml加入0．9％生理盐水250ml中静滴,1次／d,20d为一疗程。对照组：仅静滴复方苦参注射液,剂量、用法及疗程同治疗组。结果：①两组癌性疼痛疗效比较,治疗组完全缓解2例、明显缓解25例、轻度缓解10例、无效4例,有效率为65．8％;对照组有效率为40．0％,两组相比有统计学差异（P≤O．05）。②两组生存质量比较,治疗组显效7例、有效26例、无效8例,有效率为80．4％;对照组有效率为55．0％,两组有统计学差异（p≤0．05）。⑧两组体质量变化比较,治疗组体质量降低率低于对照组（P≤0．01）。结论：治疗组近期缓解率、生存质量及缓解癌痛均优于对照组,提示复方苦参注射液联合热疗较单纯应用复方苦参注射液能使较多患者临床受益。     

葛根总黄酮对骨髓瘤细胞株U266及RPMI8226增殖作用的研究

 【摘要】　目的　初步探讨葛根总黄酮（PRF）对骨髓瘤细胞株U266及RPMI 8226增殖影响及其机制。方法　采用0、10、30、50、100 μg／ml PRF分别处理U266、RPMI 8226细胞 48 h及72 h,MTT法检测细胞增殖抑制率,流式细胞术检测细胞周期变化,瑞特染色观察细胞形态学改变,FITC-Annexin V／PI双染法检测细胞早期凋亡率改变,DNA片段化分析观察PRF处理U266细胞DNA断裂片段。结果　PRF可以抑制2种骨髓瘤细胞增殖,对U266细胞抑制作用大于对RPMI 8226细胞的作用,呈浓度依赖性;随PRF浓度增加2种细胞凋亡比例增加。瑞特染色未观察到2种细胞凋亡形态学特征。0、10、30、50、100 μg／ml PRF 处理U266细胞48 h早期凋亡率分别为（3.20±0.36）%、（5.20±0.92）%、（7.30±1.22）%、（8.10±0.53）%、（10.80±0.90）%,呈剂量依赖性增高,组间差异有统计学意义（P＜0.05）。DNA片段化分析U266细胞未出现凋亡细胞特有的DNA梯带。结论　一定浓度PRF对骨髓瘤U266及RPMI 8226细胞具有较明显的增殖抑制作用;PRF对U266细胞增殖抑制作用机制虽可能与凋亡相关,但并非U266细胞增殖受抑的主要途径,其确切机制有待进一步探讨。     

Geometric Analysis of the Distolingual Root and Canal in Mandibular First Molars: A Micro–computed Tomographic Study

IntroductionThe purpose of this study was to comprehensively assess by micro–computed tomographic imaging the anatomic features of distolingual (DL) roots and canals in mandibular first molars collected from 1 population.MethodsOne hundred two specimens were examined for the location and initial direction of the DL canal and the relationship between the distance of apical deviation and the angle of root curvature.ResultsAll DL roots had only 1 canal. A new 7-category classification system is proposed for the DL roots of mandibular first molars. Most DL roots were type IV (28 teeth), type III (26 teeth), and type V (25 teeth). The average canal curvature in all root types was over 25°. There was a positive correlation between the angles of root and canal curvature for types II, IV, and V (P < .05) but not for types I and III (P > .05). In the buccolingual view, most DL roots were straight, whereas in the mesiodistal view the root curvature was close to the maximum value. The orifice of the DL canal was 4 times further from the line that bisects a line between the mesiobuccal and mesiolingual canal orifices than the distobuccal canal. The angle between the mesiobuccal-mesiolingual line and the horizontal projection of the coronal third of the DL canal on the pulp floor was 8.1° ± 10.0° and 6.1° ± 8.2° for teeth from the left and right side, respectively. The angle between the inserted simulated file and the cementoenamel junction was 57.9° ± 6.3°. Seventy-three percent of the DL canals had no constriction in the apical area. The DL canals were narrow and had a conical frustum-like shape with a 0.04 taper at the apical portion.ConclusionsThe novel classification of the DL roots helps to better understand the clinically challenging anatomy of the root and canal.     

参味合剂质量控制研究

目的建立参味合剂的质量控制方法。方法采用TLC法对制剂中五味子、麦冬、甘草进行定性鉴别;采用HPLC法测定制剂中五味子醇甲含量,色谱柱为Diamonsil C18柱（5μm,250 mm×4.6 mm）;流动相为甲醇—水（58∶42）;检测波长为250nm。结果在TLC色谱中检出五味子、麦冬和甘草,阴性对照无干扰;五味子醇甲进样量在0.3012-3.012μg范围内与峰面积呈良好的线性关系,r=0.999 99,平均加样回收率为98.55%,RSD=1.05%（n=6）。结论所建立TLC鉴别方法和HPLC含量测定方法专属性强,结果准确,重现性好,可作为参味合剂的质量控制。     

炮制对人参药材及饮片含量差异的探讨

建立人参饮片的含量测定方法,并通过测定市售人参药材及饮片的总皂苷含量,进而探讨人参饮片的质量。方法：采用HPLC测定了5批人参药材及10批饮片中人参皂苷Rb1、Re、Rg1的含量。结果：人参药材与饮片总皂苷含量差异较大,且炮制后人参饮片中人参皂苷含量降低。结论：人参药材炮制后其质量存在明显变化,亟需制定统一标准进行质量控制。     

生血宝合剂中二苯乙烯苷的含量测定

目的 建立测定生血宝合剂中君药制何首乌的有效成分二苯乙烯苷（2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷）含量的高效液相色谱（HPLC）法,为生血宝合剂的质量控制提供依据。方法 Waters alliance高效液相色谱仪,色谱柱为Suntek Kromasil C18柱（250 mm×4.6 mm,5μm）,流动相为乙腈-水（20∶80）,流速为1.0 mL/min,检测波长320 nm。结果 二苯乙烯苷在0.054 60~1.092 00μg范围内与峰面积积分值线性关系良好（R2=0.999 9）。结论 该方法灵敏度高、定量准确、方法简便、重现性好,能有效控制生血宝合剂的质量。     

Distribution of methionine-enkephalin in the minipig brainstem

We have studied the distribution of immunoreactive cell bodies and axons are containing methionine-enkephalin in the minipig brainstem. Immunoreactive axons were widely distributed, whereas the distribution of perikarya was less widespread. A high or moderate density of axons containing methionine-enkephalin were found from rostral to caudal levels in the substantia nigra, nucleus interpeduncularis, nucleus reticularis tegmenti pontis, nucleus dorsalis raphae, nucleus centralis raphae, nuclei dorsalis and ventralis tegmenti of Gudden, locus ceruleus, nucleus sensorius principalis nervi trigemini, nucleus cuneatus externalis, nucleus tractus solitarius, nuclei vestibularis inferior and medialis, nucleus ambiguus, nucleus olivaris inferior and in the nucleus tractus spinalis nervi trigemini. Immunoreactive perikarya were observed in the nuclei centralis and dorsalis raphae, nucleus motorius nervi trigemini, nucleus centralis superior, nucleus nervi facialis, nuclei parabrachialis medialis and lateralis, nucleus ventralis raphae, nucleus reticularis lateralis and in the formatio reticularis. We have also described the presence of perikarya containing methionine-enkephalin in the nuclei nervi abducens, ruber, nervi oculomotorius and nervi trochlearis. These results suggest that in the minipig the pentapeptide may be involved in many physiological functions (for example, proprioceptive and nociceptive information; motor, respiratory and cardiovascular mechanisms).     

陈宝贵教授运用有毒中药的经验

陈宝贵教授从医近50载,熟悉药物的属性及治疗病证,且配伍灵活多变,剂量随证加减得当,对有毒中药的使用亦能驾轻就熟,通过辨证论治、合理配伍、适当煎煮,对一般疾病、疑难重症、顽固沉疴无不应手奏效。