The dynamic distribution of nitric oxide synthase in the small intestine of mice with intestinal radiation sickness

ＴｈｅｄｙｎａｍｉｃｄｉｓｔｒｉｂｕｔｉｏｎｏｆｎｉｔｒｉｃｏｘｉｄｅｓｙｎｔｈａｓｅｉｎｔｈｅｓｍａｌｌｉｎｔｅｓｔｉｎｅｏｆｍｉｃｅｗｉｔｈｉｎｔｅｓｔｉｎａｌｒａｄｉａｔｉｏｎｓｉｃｋｎｅｓｓＷｅｉＬｉｃｈｕｎ（魏丽春）；ＧｕｏＹａｏ（郭鹞）（...     

SRY基因诊断在临床中的应用

应用聚合酶链式反应（ＰＣＲ）扩增技术，对１０例染色体核型为４６，ＸＹ和１例为４６，ＸＸ／４６，ＸＹ的性反转、睾丸女性化、两性畸形及男性生殖器发育不良的患者进行了性别决定区（ＳＲＹ）基因检测。结果表明９例４６，ＸＹ和１例４６，ＸＸ／４６，ＸＹ患者的ＳＲＹ基因存在，１例４６，ＸＹ女性患者的ＳＲＹ基因缺失。该结果表明能够用分子生物学技术对性反转、性发育异常的病因进行分析     

El Tor型霍乱弧菌L型的某些生物学性状

本研究主要对El Tor型霍乱弧菌(Vibrio cholerae biotvpe eltor,EVC)L型的某些生物学特性如生化反应、抗生素敏感性及超微结构等进行了探讨.研究发现EVC不稳定L型的生化性状与EVC流行株的非常相似,而稳定L型则变化很大.抗生素敏感性试验显示EVC稳定L型对某些作用于细胞壁的抗生素的敏感性减弱,而对大多数作用于蛋白质合成的抗生素的敏感性增强.与流行株相比,EVC稳定L型和不稳定L型的超微结构的改变很明显.此外,本文还讨论了产生这些改变的可能机制.     

膀胱肿瘤组织中端粒酶催化亚基和c-myc的表达

目的 研究膀胱肿瘤组织中端粒酶催化亚基(hTERT)和c-myc间的表达及关系。方法 采用逆转录-聚合酶链反应(RT-PCR)法对27例膀胱肿瘤组织、16例癌旁组织和16例正常膀胱粘膜同时测定hTERT和c-myc表达,分析两指标在3种组织的表达差异和相关性。结果 TCC组织hTERT和c-myc的表达阳性率分别为100.0％(27/27)和66.7％(18/27);癌旁组织两指标的阳性率均为 37.5％,而正常膀胱粘膜分别为 0和 25.0％;hTERT和c-myc两指标在膀胱肿瘤组织中的阳性表达有相关性(P<0.01)。结论 hTERT较c-myc作为TCC的诊断指标更灵敏和特异;两指标在TCC中的阳性表达具有相关性。     

Criminal Responsibility in Amphetamine Psychosis

Abstract: Historical changes in forensic psychiatric evaluation on criminal responsibility and proceedings in psychopathological findings of amphetamine psychosis are reviewed at first. The classification of amphetamine related mental disorders are proposed in 6 types. Among them, the clinical characteristics and psychopathological features of “Anxiety-situational reaction type” (Fukushima) are described. According to some reasonable grounds, offenders diagnosed as anxiety-situational reaction type should be evaluated as diminished responsibility in place of irresponsibility. Finally, two cases of murder committed under the influence of amphetamine, are reported in detail.     

Characteristics of peripheral blood lymphoid subpopulations of shigellosis patients

Chelyabinsk Medical Institute. (Presented by Academician of the Academy of Medical Sciences of the USSR A. D. Ado.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 113, No. 3, pp. 299–301, March, 1992.     

Low levels of essential fatty acids are related to impaired delayed skin hypersensitivity in malnourished chronically ill elderly people

Abstract Essential fatty acid (FA) deficiency, which may accompany protein-energy malnutrition (PEM), has been associated with impaired inflammatory reactions. We evaluated this relationship by analysing FA profiles and delayed cutaneous hypersensitivity in 20 malnourished elderly non-cancer patients and in 20 age-matched control patients. As indicated by serum cholesterol and serum triglycerides, the lipid levels were decreased by about one-third in the subjects with PEM. In comparison with the controls, there was a reduction in the ω 3 FA (e.g. eicosapentanoate) in total serum lipids (mgl^-1) and serum phospholipids (%) of 40% and 47%, respectively. Reductions in serum ω 6 FA (e.g. linoleate and arachidonate) levels corresponded to the drop in total FA concentrations (30%). The cutaneous hypersensitivity was impaired in 14 of the malnourished patients. The magnitude of the skin reaction was positively correlated ( P < 0·05) to the concentrations of eicosapentanoate in serum lipids and serum phospholipids, as well as to the linoleate concentration in total serum lipids. Six of the malnourished patients took part in a nutritional intervention programme for 3 months. In parallel with an improvement in the nutritional status there was a 35% increase ( P < 0·05) in the total ω 3 FA serum concentration. Negative skin tests became positive and the median skin induration enlarged threefold ( P < 0·05). Thus, deficiency of ω 3 FA might be one factor contributing to cutaneous anergy in elderly malnourished patients.     

Graft versus graft and graft versus host reactions after HLA-identical bone marrow transplantation in a patient with severe combined immunodeficiency with transplacentally acquired lymphoid chimerism

We describe a patient with severe combined immunodeficiency and transplacental transfer of maternal T cells who received an unfractionated HLA-identical sibling bone marrow transplant without prior conditioning. He presented prior to transplantation with a dermatitis later diagnosed as mild graft versus host disease. He had a normal absolute lymphocyte count, but proliferative responses to mitogens were very low. Antigens of the noninherited maternal HLA haplotype were detected on his blood lymphocytes. After transplantation, he developed a severe reaction including fever, cutaneous erythema and hepatosplenomegaly. Lymphocytes carrying the noninherited maternal HLA haplotype disappeared from his circulation, and his unprimed mononuclear cells became spontaneously cytotoxic to maternal lymphoblasts. He subsequently developed a lymphocytosis of 69,000/mm³, diarrhea, elevated transaminases and a worsening rash, necessitating treatment with immunosuppressive agents. Full T-cell engraftment and evidence of B-cell function later ensued and spontaneously cytotoxic lymphocytes against maternal cells disappeared by 47 days post-transplantation. We postulate that the patient's constellation of signs and symptoms after transplantation represented a combination of severe graft versus graft and mild graft versus host reactions.