高效液相色谱法测定利培酮片含量及有关物质

目的建立高效液相色谱法测定利培酮片的含量及有关物质.方法色谱柱为C18柱,流动相为醋酸铵缓冲液(0.1mo1·L-1,pH 5.0)-甲醇-乙腈(50:30:20),检测波长为279nm.结果该法专属性好:辅料和降解产物对制剂的含量测定没有干扰;在2～100μh·mL-1浓度范围内,线性关系良好r=0.9999;检测限为0.4ng;平均回收率为98.47%;日内、日间RSD分别为0.22%和0.96%.结论该方法专属性强、灵敏度高、简便易行、结果准确,适用于利培酮制剂的含量和有关物质测定.     

奥氮平及其片剂的HPLC测定

建立奥氮平及其片剂测定的HPLC法.采用ODS C18色谱柱,0.01mol/L磷酸二氢钾溶液-甲醇-乙腈(1:1:1,pH8.3)为流动相,检测波长273nm.线性范围为0.01～1mg/ml,检测限1ng.方法平均回收率为99.4%,RSD为0.7%.     

HPLC法测定佐米曲普坦的含量及有关物质

目的:建立HPLC法测定佐米曲普坦的含量及纯度.方法:采用Zorbax SB-C18色谱柱,分别以甲醇:0.025mol/L磷酸二氢钾(16:84)和甲醇:0.025 mol/L磷酸二氢钾:三乙胺(25:75:0.5,pH=3.5)为流动相,流速为1mL/min,在检测波长为220nm处检测.结果:本品在0.01mg/mL～0.15mg/mL浓度范围内呈直线关系,本品中杂质的最小检测限为0.01%.对4批样品进行检测均未检出杂质,而粗品检出明显杂质.结论:本方法可用于含量测定和有关物质的检查.     

Integration of toxicological approaches with “omic” and related technologies to elucidate mechanisms of carcinogenic action: Propiconazole,an example

The field of mechanistic chemical carcinogenesis has evolved with the advent and advances in genomic, proteomic and metabolomic technologies. These advances allow mechanistic events along the process of exposure to frank tumors to be studied in great detail. Herein is reviewed an example of this approach using, propiconazole, a triazole-containing antifungal agent that is a mouse hepatocarcinogen. This review will highlight those toxicological, genomic, proteomic and metabolomic findings in mice that were used to describe a set of linked events that lead to propiconazole-induced hepatocarcinogenesis. Independent experimental proof of many of these events is presented that solidified this proposed mechanism of carcinogenic action for propiconazole.     

Characteristics of analytically confirmed 3-MMC-related intoxications from the Swedish STRIDA project

Abstract

Background. 3-Methylmethcathinone (3-MMC) is a synthetic cathinone stimulant structurally related to the new psychoactive substance (NPS) mephedrone (4-methylmethcathinone, 4-MMC). We describe a case series of analytically confirmed intoxications involving 3-MMC presented to emergency departments in Sweden and included in the STRIDA project. Study design. Observational case series of consecutive patients with self-reported or suspected use of NPS presenting to hospitals in Sweden between August 2012 and March 2014. Methods. NPS analysis was performed by a liquid chromatography–mass spectrometry (MS)/MS method that is updated with new substances as they appear. Data on clinical features were collected during Poisons Information Centre consultations and retrieved from medical records. Results. 3-MMC was detected in 50 (6.4%) of the 786 cases included in the STRIDA project during the 20-month study period, with the peak occurring in August 2013. The age range of patients testing positive for 3-MMC was 17–49 years (median 24) and 76% of them were men. The 3-MMC concentration in serum ranged between 0.002 and 1.49 μg/mL (median, 0.091) and between 0.007 and 290 μg/mL (median, 3.05) in urine. Co-exposure to other NPS and/or traditional drugs was very common, and 3-MMC mono-intoxication was found in only 4 (8%) cases. The most frequent clinical features were tachycardia (48% of cases) and agitation (42%). Other features included a reduced level of consciousness (32%), dilated pupils (24%), hallucinations (20%), diaphoresis (12%), seizures (8%), and hyperthermia (6%). Most patients (60%) needed hospital care for only 1 day but in 8% for 3 days or longer. Conclusion. The majority of patients with analytically confirmed 3-MMC exposure had sympathomimetic features similar to those associated with mephedrone intoxication. However, the high incidence of co-exposure to other drugs makes the clinical interpretation difficult. Nevertheless, 3-MMC was associated with a high admittance rate to intensive care (30%), and detected in two cases with a fatal outcome, suggesting that 3-MMC is a harmful drug.     

Replacement therapy with piperazine oestrone sulphate('Harmogen') and its effect on memory

Summary

A formal memory test was administered to 18 female patients with signs or symptoms of oestrogen deficiency taking part in a double-blind study of piperazine oestrone sulphate. A significant improvement in memory was seen in the treated group compared with the placebo group. The findings are discussed.