Hyperosmolarity response of ocular standing potential as a clinical test for retinal pigment epithelium activity rhegmatogenous retinal detachment

The hyperosmolarity response of the ocular standing potential was recorded in unilateral rhegmatogenous retinal detachment (8 eyes) and in the fellow healthy eye (8 eyes). The hyperosmolarity response was greatly suppressed (M-4 SD: M and SD indicate respectively the mean and the standard deviation in normal subjects) in all affected eyes (p < 0.005), and slightly abnormal in 2 fellow eyes. The L/D ratio was normal in 2 affected eyes and in all fellow eyes. The hyperosmolarity response in the affected eyes was still greatly suppressed 14 months after successful surgical treatment.     

Optimum support properties for protein separations by high-performance size exclusion chromatography

Optimum chromatographic properties of high performance size exclusion chromatography (HPSEC) of proteins, such as resolution, molecular weight accuracy and recovery, are obtained on packings and columns with tailor-made physical and chemical structures, employed at properly adjusted eluent compositions and operation conditions. SEC-theory suggests that a broad molecular weight fractionation range and high linearity of the log-linear calibration plot can be achieved by the use of two packings (10- and 80-nm pore size, characterized by a pore-size distribution (psd) equal to or less than 1 decade and by equal internal column porosity (p)), rather than a single 30- to 50-nm pore-size packing with a wide psd. Favourably high-phase ratios of (p)/(o)/ 1.0 for HPSEC columns were accomplished with a minimum interstitial column porosity (o) and a high value for the internal column porosity (p) (the specific pore volume, nu(p), multiplied by the packing density, varrho(p).) Ligands such as diol, N-acetoxyamino and oligomeric ether with a propyl- or propoxy-spacer bonded to the silica at the highest density appear to provide high mass recovery and bioactivity as well as chemical stability. Such packings, available in 3-5 mum particle size ranges of narrow distribution, packed into columns 6 mm i.d. and 500 mm in length, offer the best compromise with respect to resolution, speed and pressure drop. More careful studies are required to explain the effects of protein conformational changes and interconversions during elution on HPSEC columns.     

25-Hydroxyvitamin D and Cardiorespiratory Fitness in Prepubertal Overweight and Obese Children

Childhood obesity has become a major global health problem. Vitamin D deficiency and poor cardiorespiratory fitness are highly prevalent in children with overweight or obesity, but little is known about their relationships. In this study, we aimed to analyze the relationship between serum 25-hydroxyvitamin D (25(OH)D) and cardiorespiratory fitness parameters in prepubertal obese and overweight children. A cross-sectional design with a sample of 57 prepubertal children, aged 9–11 years, with overweight or obesity was used. The fasting concentration of 25(OH)D was analyzed with a chemiluminescent microparticle immunoassay. Fat and lean body masses were determined by using DXA. Maximal oxygen uptake (VO_2max) was measured with the maximal treadmill test. A total of 68.4% of the sample had sufficient levels of 25(OH)D. As expected, their cardiorespiratory fitness was poor compared with that of normal-weight children, but 60% of the group exceeded the median obesity-specific reference values. No differences were found between the sexes for relative VO_2max or 25(OH)D levels. Moreover, no correlations were found between 25(OH)D and body composition or cardiorespiratory parameters for sex or vitamin D groups. Vitamin D status seems not to be directly related to body composition or cardiorespiratory fitness in prepubertal overweight or obese children.     

心肌多普勒组织显像与心肌增厚率相关分析

目的：研究脉冲多普勒组织显像评价室壁运动的可行性。方法：对19例研究对象左室基底段心肌进行脉冲多普勒组织显像并测量最大收缩速度及心肌收缩平均加速度和在同一部位的M-mode运动轨迹上测量心肌增厚率,对其进行相关回归分析。结果：心肌脉冲多普勒显像的最大收缩速度与M-Mode测量的心肌增厚率呈中度相关（r=0.55,P＜0.05),脉冲多普勒组织显像的心肌收缩平均加速度与M-mode的心肌增厚率呈中度相关（r=0.56,P＜0.05)。结论：脉冲多普勒组织显像显示的心肌最大收缩速度和心肌收缩平均加速度可作为评价室壁节段运动的指标。     

老年单纯收缩期高血压患者血脂及血浆同型半胱氨酸水平的变化

目的：研究老年单纯收缩期高血压（ISH）患者血浆同型半胱氨酸（Hcy)与胆固醇（Tc),甘油三脂（Tg)水平的变化及其相关性。方法：运用高效液相色谱仪及氧化酶法分别测定35例ISH患者,31例单纯期高血压患者,34例原发性高血压患者及30例健康对照组血浆Hcy,Tc,Tg水平,结果：（1）单纯收缩期高血压患者,舒张期高血压患者,原发性高血压患者的Tc,Tg水平均高于健康对照组,差异有显著性意义（P＜0．01）,而Hcy水平只有单纯收缩期高血压和原发性高血压患者高于健康对照组,差异有显著性意义（P＜0．01）;舒张期高血压患者的Hcy水平与健康对照组相比无显著性差异（P＞0．05）,（2）单纯收缩期高血压患者的Tc,Tg水平与舒张期高血压患者,原发性高血压患者的Tc,Tg水平相比没有显著性差异（P＞0．05）,但单纯收缩期高血压患者和原发性高血压患者,原Hcy水平却高于舒张期高血压患者,差异有显著性意义（P＜0．01）,（3）Hcy与Tc,Tg均呈正相关,相关系数分别为0．355（P＜0．01）,0．389（P＜0．01）。结论：老年ISH患者和原发性高血压患者血浆Hcy水平较舒张期高血压患者和健康正常对照组均明显增高,且血浆Hcy和Tc,Tg间有一定的相关性。     

用彩色M型多普勒测量舒张早期右室内血流传播速度评价右室舒张功能

目的 :探讨右室舒张早期血流传播速度评价心脏右室舒张功能的价值。方法 :应用彩色M型多普勒测量 6 5例正常人 ,80例高血压 ,30例冠心病患者的右室内舒张早期血流传播速度。同时用脉冲多普勒测量了三尖瓣口的血流速度频谱。结果 :正常人右室内血流传播速度分别是 (4 0 .5± 8.8)cm/s(4 0～岁 ) ,(37.92± 5 .45 )cm/s(5 0～岁 ) ,(36 .4± 4.6 )cm/s(6 0～岁 )。高血压和冠心病右室内血流传播速度分别为 (2 4.9± 8.9)cm/s和 (2 4.5± 7.2 )cm/s,比正常人明显降低 (P <0 .0 0 1)。结论 :应用彩色M型多普勒测量的右室内血流传播速度能简便、准确地评价右室舒张功能     

In Vitro Validation of Tissue Doppler Left Ventricular Regional Wall Velocities by Using a Novel Balloon Phantom

Velocity data from tissue Doppler imaging (TDI)can provide valuable information on regional leftventricular wallmotion. Validation of TDImyocardialvelocity measurements has been carried out indirectlyfrom gray- scale M- mode images,and discrepancieshave been reported.Mc Dicken[1] and Miyatake etal[2 ]have reported the accuracy and validity of the TDIsystem using a rotating sponge model.However theoverall motion of the heart should be considered.Inthe present study,we described a new TDI…     

重组人生长激素治疗生长激素缺乏症86例疗效分析

目的研究国产和进口重组人生长激素(rhGH)治疗生长激素缺乏症(GHD)的临床效果.方法应用国产rhGH治疗GHD 67例,身高(118.4±14.4)cm,身高SDS-(4.1±1.6).生长速率(3.0±0.8)cm/年.进口rhGH 19例,身高(129.3±9.0)cm,身高SDS-(5.5±1.6),生长速率(2.8±1.1)cm/年.rhGH,每公斤体重每日0.1IU,疗程6个月.结果 rhGH对这些患儿体格的线性生长有显著促进作用(P＜0.001),国产rhGH治疗6个月生长速率(12.6±3.6)cm/年,进口rhGH(12.0±2.2)cm/年,两者差异无显著性应用国产rhGH的完全性GHD和部分性GHD6个月的生长速率分别为(13.4±3.7)cm/年和(10.3±2.2)cm/年,两者差异有显著性(P＜0.002).本文86例中,骨龄≤5岁和≥7岁6个月生长速率分别为(14.1±3.3)cm/年和(11.     

甘肃省疾病预防控制系统慢性病防控基础配置能力评估

摘 要：目的：探讨社区诊室血压达标的高血压患者晨峰血压控制及血压管理效果评估。方法：将400例高血压患者随 机分为研究组和对照组。试验前后完善24 h动态血压、24 h动态心电图及头颅CT检查,随后每3个月行1次24 h动态血压检 查。生活方式和药物干预 12个月,观察两组治疗前后血压达标情况及心脑血管事件发生率。结果：研究组治疗中后期患者 24 h平均收缩压、起床后2 h内平均收缩压及晨峰血压均较同期对照组显著降低;两组心脑血管疾病发病率差异有统计学意 义 （χ2=5.171,P＜0.05）。结论：诊室血压达标伴晨峰血压的患者更易发生心脑血管疾病,采用适宜的血压管理模式可有效 降低晨峰血压。     

Biomechanical assessment with electromyography of post-stroke ankle plantar flexor spasticity

Spasticity has been defined as a motor disorder characterized by a velocity-dependent increase in tonic stretch reflex (muscle tone). Muscle tone consists of mechanical-elastic characteristics, reflex muscle contraction and other elements. The aims of this study were to determine whether to assess spasticity quantitatively, and to characterize biomechanical and electromyographic spasticity assessment parameters. These assessment parameters were described by investigating the correlation between clinical measures and the response to passive sinusoidal movement with consecutive velocity increments. Twenty post-stroke hemiplegic patients and twenty normal healthy volunteers were included in the study. Five consecutive sinusoidal passive movements of the ankle were performed at specific velocities (60, 120, 180, and 240 degrees/ sec). We recorded the peak torque, work, and threshold angle using a computerized isokinetic dynamometer, and simultaneously measured the rectified integrated electromyographic activity. We compared these parameters both between groups and between different velocities. The peak torque, threshold angle, work, and rectified integrated electromyographic activity were significantly higher in the post-stroke spastic group at all angular velocities than in the normal control group. The threshold angle and integrated electromyographic activity increased significantly and linearly as angular velocity increased, but the peak torque and work were not increased in the post-stroke spastic group. Peak torque, work, and threshold angle were significantly correlated to the Modified Ashworth scale, but the integrated electromyographic activity was not. The biomechanical and electromyographic approach may be useful to quantitatively assess spasticity. However, it may also be very important to consider the different characteristics of each biomechanical parameter.