阿霉素明胶微球的制备与特性研究

目的 :对动脉栓塞阿霉素明胶微球 (ADM GMS)的制备工艺及特性进行初步研究。方法 :以生物降解材料明胶为载体 ,采用乳化化学交联法制备含阿霉素的明胶微球 ,并进行家兔胃左动脉栓塞 ,分别于术后 1周至 4周内作栓塞观察。结果 :所制备的阿霉素明胶微球外形圆整 ,平均粒径为 6 2 .74μm ,载药量为2 1.78μg·mg-1,包封率为 6 7.43% ,体外释药和动物胃左动脉栓塞基本符合要求。结论 :阿霉素明胶微球有望成为临床用动脉栓塞术治疗胃癌的新给药制剂。     

明胶软胶囊囊壳处方因素对囊壳溶解性能的影响

 目的：考察明胶软胶囊囊壳处方因素对囊壳溶解性能的影响。方法：以柠檬黄为指示剂,按常规方法制备明胶胶块及胶片,以溶出度测定方法,测定了胶片的溶解速率。结果：随着明胶/甘油比例的增加,制得胶片的溶解速率呈不规则变化,胶片经40℃贮存21d,各种处方的胶片溶解速率呈不同程度下降。胶块处方中加入高分子材料PVP、淀粉可轻微增加明胶的溶解速率。结论：对各种因素的讨论有助于设计各种用途的软胶囊囊壳。并提示明胶胶囊应尽可能在低温保存。     

骨基质明胶诱导成骨的组织学特点

目的：探讨骨基质明胶诱导成骨的组织学特点。方法：在成年家兔双侧桡骨干中段制成骨缺损模型,分别植入无菌生理盐水和骨基质明胶。采用组织形态学方法,观察骨基质明胶的成骨过程及修复骨缺损的能力。结果：骨基质明胶可诱导间充质细胞聚集,并促进其分化为软骨细胞、成骨细胞进而形成新骨。结论：骨基质明胶是通过诱导机理促进形成新骨的;诱导成骨是软骨内成骨过程。     

Immune complex transfer enzyme immunoassay for (anti-human T-cell leukemia virus type I) IgG in serum using a synthetic peptide, Cys-Gag p19(100-130), as antigen.

An immune complex transfer enzyme immunoassay for (anti-human T-cell leukemia virus type I) IgG (anti-HTLV-I IgG) in serum using a synthetic peptide, cys-gag p19(100-130), is described. Anti-HTLV-I IgG in test serum, which had been incubated with excess of inactive beta-D-galactosidase to eliminate interference by anti-beta-D-galactosidase antibodies, was reacted simultaneously with 2,4-dinitrophenyl bovine serum albumin-cys-gag p19(100-130) conjugate and cys-gag p19(100-130)-beta-D-galactosidase conjugate. The complex formed of the three components was trapped onto polystyrene balls coated with affinity-purified (anti-2,4-dinitrophenyl group) IgG. After washing to eliminate nonspecific IgG in the test serum and excess of the beta-D-galactosidase conjugate, the complex was eluted from the polystyrene balls with epsilon N-2,4-dinitrophenyl-L-lysine and transferred to polystyrene balls coated with affinity-purified (anti-human IgG gamma-chain) IgG. Beta-D-Galactosidase activity bound to the (anti-human IgG gamma-chain) IgG-coated polystyrene balls was assayed by fluorometry. This assay was 100-fold more sensitive than the conventional enzyme immunoassay, in which a cys-gag p19(100-130)-bovine serum albumin-coated polystyrene ball was incubated with test serum and, after washing, with (anti-human IgG gamma-chain) Fab'-peroxidase conjugate. The degree of inhibition by preincubation of test sera with excess of cys-gag p19(100-130) in combination with an appropriate cut-off value for the fluorescence intensity of bound beta-D-galactosidase activity discriminated almost all seropositive samples from seronegative ones.     

Modification of Gelatin Beadlets for Zero-Order Sustained Release

A three-phase suspension process was used for the preparation of gelatin beadlets containing succinylsulfathiazole. When the beadlets were hardened with 10% formalin at 5°C for varying periods of time up to 24 hr, the 6-hr hardening time gave the slowest release rate. Drug release rate from gelatin beadlets was slower in simulated gastric fluid (SGF) than in simulated intestinal fluid (SIF) but the sustained effect was too limited to be useful for most applications. When the hardened gelatin beadlets were coated with cellulose acetate butyrate (CAB) by an emulsion–solvent evaporation method, a more pronounced sustained effect and a nearly zero-order release were found in SIF. The effects of the amount of gelatin used, the amount of CAB employed, and the length of hardening time on drug release were investigated. The treatment of gelatin beadlets with formalin reduced the swelling action of gelatin in aqueous medium. A nonzero-order drug release rate was observed when the gelatin swelled sufficiently to rupture the CAB coating. The drug release rate can be adjusted by using different ratios of hardened gelatin beadlets and CAB coating in which the gelatin enhances the release rate and the CAB serves as a barrier.     

用于培养动物细胞微载体的制备

合成了两种用于动物细胞培养的微载体:葡聚糖和明胶微载体。报道了合成方法。将产物初步用于Vero细胞培养,得到了满意的结果。细胞在微载体上的附着生长良好,和目前广泛使用的Cytodex 1微载体效果相仿。当DEAE-Sephadex 1.5微载体浓度为2mg/ml时,在1.5L Celligen细胞培养器中培养120小时,细胞浓度可达1.2×10~6细胞/毫升,为接种浓度的5倍,达到了文献中报道的水平。     

Development and Characterization of Active Gelatin Films Loaded with Rapeseed Meal Extracts

The use of industrial waste as a material for the development of natural innovative and active packaging is economically and environmentally appealing. The aim of this study was to develop and characterize active gelatin films incorporating rapeseed oil industry waste. Water (RM-WE) and methanolic (RM-MWE) extracts of rapeseed meal (RM) were used as active agents in film formulations. The active films were produced by a casting technique. The physicochemical, mechanical, optical, morphological, radical scavenging, and antibacterial properties of the films were analyzed. The addition of RM-WE and RM-MWE in the concentrations range between 4 and 12% promoted an increase of Young’s modulus (YM) and radical scavenging properties of films investigated by the direct QUick, Easy, New, CHEap and Reproducible procedure using 2,2-diphenyl-1-picrylhydrazyl (QUENCHER_DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (QUENCHER_ABTS) radicals. The antibacterial properties of films were examined against five bacterial strains: E. coli, S. enterica, M. luteus, L. monocytogenes, and S. aureus. Additionally, color and opacity of the control and fortified films differed significantly. The gelatin films with RM extracts are resistant to the microbial spoilage and could be used to produce active packaging for food that is vulnerable to rancidity effects.     

诺迪康胶囊治疗急性期肺心病的临床试验

目的：观察诺迪康胶囊对急性期肺心病的治疗效应，以及对SPAP的影响。方法：选择住院的急性期肺心病患者40例，随机分为常规治疗组和诺迪康组，各20例。两组均经抗感染及吸氧等常规治疗。诺迪康组在常规治疗的基础上加用诺迪康胶囊，疗程共14天。治疗前后观察症状、体征和SPAP的变化。结果：诺迪康组较常规治疗组显效率更高，两组SPAP均有下降，但诺迪康组下降更明显。结论：诺迪康胶囊配合常规治疗可提高治疗急性期肺心病的疗效，具有降低SPAP的作用。     

Hydrolytic Stability of Methacrylamide and Methacrylate in Gelatin Methacryloyl and Decoupling of Gelatin Methacrylamide from Gelatin Methacryloyl through Hydrolysis

Gelatin methacryloyl (GelMA; GM) is a promising nature‐derived photocurable material that can mimic the extracellular matrix because GelMA features tailorable mechanical properties, proteolytic degradation, and good cell adhesion. GelMA contains not only methacrylamide but also methacrylate. However, the hydrolytic stability of methacrylamide and methacrylate groups of GelMA in aqueous solutions has not been scrutinized. Here, the structural change of GelMA through hydrolysis is investigated for the first time. The structural change of hydrolyzed GelMA is quantitatively identified using colorimetric and ¹H NMR methods. The methacrylate groups decompose markedly at high pH solutions, but the methacrylamide groups remain stable. Further, pure gelatin methacrylamide is successfully decoupled from GelMA for a better understanding of GelMA structure and future use for biomedical applications.