CpG ODN增强乙肝疫苗在老年小鼠中的免疫应答

目的：探讨CpG ODN对老年小鼠的体液和细胞免疫应答的增强作用。方法：选用老年C57BL／6小鼠，将乙肝疫苗和10μg、20μg CpG ODN同时或单独肌注到小鼠体内，两周后以同样剂量加强免疫一次，再过3周后摘除眼球取血，用EILSA方法检测抗HBs16；抗体和IL-12；无菌取脾脏作HE染色，观察脾脏淋巴细胞变化。结果：10μg和20μg CpG ODN与疫苗同时注射组产生的抗体绝对量分别是单独注射疫苗组的3倍和4倍；产生的IL-12水平较对照组有明显升高，且20μg比10μCpG组产生的IL-12水平更高。光镜下各组的脾脏淋巴细胞的变化如下：正常老年鼠组脾脏淋巴细胞较正常青年鼠组明显稀少；老年鼠 10μgCpG组脾脏淋巴细胞较正常老年鼠组有了明显增加，且细胞核也明显增大；20μgCpG组增加的更加明显。结论：CpG ODN能增强乙肝疫苗在老年小鼠中的体液和细胞免疫应答。     

灵芝多糖对小鼠糖耐量的影响

目的 :探讨灵芝多糖对正常和糖尿病小鼠糖耐量的影响。方法 :采用腹腔注射四氧嘧啶 (2 0 0 mg· kg- 1 )诱导小鼠造成糖尿病模型 ,分别观察单次给予 0 .5 g·kg- 1、1.5 g· kg- 1灵芝多糖后 ,正常和糖尿病小鼠糖耐量的改变。结果 :灵芝多糖 0 .5 g· kg- 1仅能显著降低糖尿病小鼠餐后 1h和 2 h血糖 ,而灵芝多糖 1.5 g·kg- 1对正常和糖尿病小鼠餐后 1h和 2 h血糖均有显著降低作用。结论 :灵芝多糖能改善正常和四氧嘧啶糖尿病小鼠糖耐量。     

江西麦饭石对某些实验动物生理功能影响

用江西麦饭石水浸液喂养小鼠116只,自来水喂养39只,食物条件完全相同,一个月后,发现饮用江西麦饭石水浸液的小鼠体重增长和抗疲劳及耐缺氧能力均优于对照组。用麦饭石任氏液灌注离体蛙心后,心肌收缩力有明显增强。     

Response of V beta 8.1+ T cell clones to self Mls-1a: implications for the origin of autoreactive T cells.

Clonal deletion and anergy are two major mechanisms of self-tolerance. However, the molecular mechanisms underlying clonal deletion and anergy, as well as the threshold of TCR affinity/avidity required for these processes, are not known. Expression of the V beta 8.1 TCR correlates with the reactivity of the T cells to the minor lymphocyte stimulating locus-1a (Mls-1a) and T cells expressing this TCR are deleted in the thymus of Mls-1a mice. Similarly, in TCR V beta 8.1 transgenic mice, the number of CD4+CD8-T cells is reduced in Mls-1a mice. However, small numbers of CD4+CD8-T cells remain in the periphery of adult Mls-1a transgenic mice. We have generated T cell clones from TCR V beta 8.1 transgenic mice by stimulation of lymph node T cells with C57BL/6 alloantigens. Interestingly, CD4+CD8-V beta 8.1+ clones isolated from the transgenic mice of Mls-1a background responded to the self-antigen Mls-1a, to which they did not respond in primary assay. Reactive patterns of the clones were compared with clones derived from Mls-1b mice. Proliferation and cytokine production of the clones from Mls-1a mice to the self-antigen Mls-1a were generally reduced when compared with clones from Mls-1b mice. More importantly, T cell clones from Mls-1a mice required more Mls-1a antigen for their activation, and were more susceptible to the inhibitory effects of anti-CD4 antibody on the proliferative responses to Mls-1a than those from Mls-1b mice. These results suggest that the T cell receptor on clones derived from Mls-1a mice have functional but reduced affinity/avidity for self-antigen Mls-1a.     

rhIL－1α对小鼠骨髓细胞长周期培养中造血细胞增殖的影响

应用小鼠骨髓细胞长周期培养（ＬＴＢＭＣ）体系，观察重组人白细胞介素－１α（ｒｈＩＬ－１α）对造血细胞增殖的影响。结果显示，经ｒｈｌＬ－１α作用后，小鼠ＬＴＢＭＣ中悬浮细胞数增多，其中粒单系祖细胞（ＣＦＵ－ＧＭ）数量也明显增加，维持时间延长。表明ｒｈＩＬ－１α能促进小鼠造血细胞在体外ＬＴＢＭＣ中增殖并分化，而且与剂量有关系。ｒｈＩＬ－１α加入的时间，以骨髓细胞２次接种时同时加入为好，延迟加入则作用减弱。本实验对骨髓干、祖细胞体外扩增和自体骨髓移植等方面的研究有一定意义。     

ICR 小白鼠脏器中铜,锌锰含量

对40只正常成年远交系 ICR 小白鼠的主要脏器,用 WFX—Ⅱ型双光束原子吸收分光光度计测定了各脏器中铜、锌、锰含量.结果表明,主要脏器中铜、锌、锰含量大小顺序不一,铜含量最多的脏器是心,锌、锰含量最多的脏器是肝,在雌雄之间均无显著性差异.     

25％乙基硫环磷农药的诱变性研究

本文对新型有机磷农药，２５％乙基硫环磷进行了Ａｍｅｓ及微核两项诱变性试验研究。Ａｍｅｓ试验中，无论是否加入Ｓ９活化系统，２５％乙基硫环磷均未能诱发回变菌落数的增加；微核试验的各剂量组ＰＣＥ微核率也较低，（２‰～３‰），与阴性对照相比无显著性差异。     

急性苯中毒对小鼠末梢血象和骨髓细胞构成的影响

取３２只２～３个月的ＢＡＬＢ／Ｃ小白鼠，随机分为４组，每组８只，第１组为对照组，其余３个组为实验组，实验组小白鼠左腿皮下注射苯０．３ｍｌ（１５ｍｌ／ｋｇ体重）．注射后分别于２４ｈ，４８ｈ，７２ｈ脱颈处死。观察中毒前、后末梢血象、骨髓细胞、骨髓细胞构成的变化。结果表明，苯中毒时骨髓变化先于末梢血象的变化，骨髓的病变为造血细胞变性、坏死，大量毛细血管扩张、充血，骨髓细胞构成降低，而且骨髓细胞构成中细胞成分降低早于外周血粒细胞减少。     

不同免疫缺陷小鼠巨噬细胞功能的初步研究

本文着重检测了不同免疫缺陷的纯合子615-nu、615-bg、615-nu/bg、Ba1b/c-nu和表现型正常的杂合子615/PBI小鼠的巨噬细胞细胞毒活性、吞噬功能,并与它们的NK细胞活性和T、B细胞功能进行了对比分析。实验结果表明:这些不同免疫缺陷的615小鼠其相应的免疫缺陷的性质是确定的。615-nu、615-nu/bg、Ba1b/c-nu的巨噬细胞毒活性与615/PBI无明显差别,但吞噬指数较正常升高,而615-bg的巨噬细胞细胞毒活性和吞噬功能则都显著低于615/PBI的。