碘标记神经生长因子在缺氧新生鼠脑内的吸收

目的观察碘标记神经生长因子(125I-NGF)在缺氧新生鼠脑内的吸收情况。方法选取32只7日龄健康新生鼠随机分成实验组(125I-NGF组和125I组)及正常组(125I-NGF组和125I组),实验组制成缺氧缺血性脑损伤(HIBD)模型。分别予125I-NGF和单纯125I腹腔注射后30 min处死取脑,按如下解剖分出基底前脑、额皮质、海马、丘脑下区、小脑、嗅球、垂体,对标本进行放射性测量。结果两125I NGF组于基底前脑、小脑、额皮质、海马、嗅球均有显著吸收,且NGF在缺氧鼠额皮质、海马、小脑吸收显著高于NGF在正常新生鼠以上部位。结论外源性NGF可透过新生鼠血-脑脊液屏障,早期应用治疗HIE是可行的。     

窒息后缺氧缺血性脑病新生儿脐血S-100B蛋白变化的意义

目的研究脐血S-100B蛋白在窒息新生儿中的变化,探讨其对新生儿窒息后缺氧缺血性脑病(HIE)的早期诊断价值。方法同期分娩足月正常新生儿43例为对照组(1组)。Apgar评分<7分未发生HIE 44例,为窒息组(2组);发生HIE 16例,为HIE组(3组)。用ELISA方法分别检测3组脐静脉血S-100B水平。结果1~3组S-100B蛋白水平分别为(1.055±0.356)(、1.572±0.533)(、2.394±0.943)μg/L。2、3组脐血S-100B较1组显著增高(t=2.306,7.991 P均<0.01)。3组脐血S-100B较2组显著增高(t=4.242 P<0.01)。结论窒息新生儿脐血S-100B蛋白较正常升高,其可作为窒息后HIE早期诊断指标之一。     

199例新生儿溶血病血液血清学检测结果分析

目的了解黄疸患儿中溶血病 （HDN）的发病率.方法血清学检查参照《输血技术手册》进行.结果 199例新生儿黄疸患儿的血清学检查中,阳性89例,其中ABO系统 85例,Rh系统4例.结论对新生儿黄疸患儿的血清学检查,可早期明确诊断,提高治愈率.     

新生儿重症监护病房中遗传代谢病的临床特点及分析

目的提高临床医生对重症监护病房新生儿期发生的遗传代谢病的认识，提高早期诊断率。方法对2005年5月-2006年2月NICU中新生儿早期原因不明的严重酸中毒、高血钾、猝死、窒息等进行尿气相色谱-质谱仪检测；同时部分检测血乳酸、丙酮酸、血氨等。结果7例早期新生儿分别于出生时15小时、2天、3天、4天、6天发病，诊断为先天性丙酮酸代谢障碍1例、同型半胱氨酸血症1例、有机酸血症2例、戊二酸尿症Ⅱ型1例、酪氨酸血症1例、先天性肾上腺皮质增生症1例。结论NICU应警惕早期新生儿遗传代谢病，可利用目前的技术力量提高诊断率。     

Biomarkers in neonatology: the new “omics” of bronchopulmonary dysplasia

Bronchopulmonary dysplasia (BPD) is a complex disorder resulting from gene–environmental interactions. An improved understanding of the pathogenesis of this most common chronic lung disease in infants has been made by utilizing animal models and correlating with human data. Currently, while some (vitamin A, caffeine) pharmacotherapeutic options are being utilized to ameliorate this condition, there is still no specific or effective treatment for BPD. It would be helpful for prognostication and targeted potential novel therapeutic strategies to identify those babies accurately who are at risk for developing this disease. A reliable biomarker would have the capacity to be detected in the initial phase of the disease, to allow early interventions to avoid or minimize the detrimental effects of the disease. This review will focus on human studies performed with the “omic” techniques, specifically genomics, epigenomics, microbiomics, transciptomics, proteomics and metabolomics, and summarize the information available in the literature, as it pertains to biomarker identification for BPD. Using “omics” technologies, investigators have reported markers that have the potential to be used as biomarkers of BPD: SPOCK2, VEGF ?624C?>?G, VEGF ?460T?>?C, mast cells specific markers, miR-219 pathway, miR-152, ?30a-3p, ?133b, ?206, ?7, lactate, taurine, trimethylamine-N-oxide, gluconate, myoinositol and alterations in surfactant lipid profile.     

Newborn Screening for Hemoglobinopathies and Red Cell Enzymopathies in Tripura State: A Malaria-Endemic State in Northeast India

Hemoglobinopathies are a group of inherited single gene disorders. There are reports on hemoglobin (Hb) variants identified in the tribal and non-tribal populations of Tripura State in northeastern India. This study aimed to determine the spectrum of hemoglobinopathies and enzymopathies by newborn screening in Tripura State and assess the extent of neonatal jaundice. A total of 2400?cord blood samples were collected and analyzed by high performance liquid chromatography (HPLC). Further confirmation of any abnormal HPLC was done by DNA analysis. The samples were also screened for deficiency of enzymopathies, glucose-6-phosphate dehydrogenase (G6PD) deficiency and pyruvate kinase. Of 2400?cord blood samples screened, 225 (9.3%) were Hb E (HBB: c.79G>A) heterozygotes, 80 (3.3%) were Hb E homozygotes and one carried Hb E-β-thalassemia (β-thal). Other Hb abnormalities were also detected including 15 Hb S (HBB: c.20A>T) heterozygotes, two Hb D-Punjab (HBB: c.364G>C) heterozygotes and two compound heterozygotes for Hb D-Punjab and Hb E. Of the 80 homozygous Hb E babies, four were non-tribal and 76 babies were tribal, and 225 patients carried Hb E trait, 141 were tribal, while 84 were non-tribal. Of 40 G6PD deficient babies identified, 13 had coinherited Hb E and two babies had pyruvate kinase deficiency. α Genotyping was performed in 162 affected babies, 50 of them carried α gene deletions. Newborn screening programs for Hb E, other hemoglobinopathies and G6PD deficiency must be encouraged in the malaria-endemic northeastern region of India. Drug-induced hemolysis can also be avoided by screening for G6PD deficiency at birth.