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971.
观察自发性高血压大鼠(SHR)的甲状旁腺。发现在其腺体中除大量正常的主细胞外,还具有一种特殊的新型细胞。这些细胞的形态不规则,边界不清。HE染色后胞浆比主细胞着色深,核的形态不规则且染色更暗。此种细胞在甲状旁腺中成团状分布,与主细胞形成鲜明的对比。另外,经实验证实在SHR的血浆中存在一种特殊的高血压因子(HF),可以使正常大鼠的血压产生延迟性升高,其高峰在45min出现,此特点与血中已知的增压物质的即刻效应和延期出现的效应有本质的不同。因此,推论这种具有独特的理化性质和生理功能高血压因子的来源与甲状旁腺有密切关系。 相似文献
972.
Mice expressing transgenic T cell receptors (TCR) are used to explore important questions in immunity. However, transgene expression may have unexpected effects. We previously reported a B cell immunodeficiency, comprising decreased B cell numbers and diminished antibody responses, in mice that express a transgenic TCR specific for nicotinic acetylcholine receptor; the mice were generated using cassette vectors designed specifically for transgenic TCR expression [see Kouskoff et al. J. Immunol. Methods 1995. 180: 273-280]. We now show data suggesting that this defect is due to the expression and accumulation of TCR alpha and beta chains inside B cells and induction of an endoplasmic reticulum stress response, causing apoptosis at the pre B-I and later B cell stage. Thus, inappropriate transgene expression can profoundly affect B cells, leading to a previously undescribed mechanism of immunodeficiency. 相似文献
973.
M. Ishizawa K. Matsumoto S. Kukuda H. Okabe K. Hodohara S. Ota 《Virchows Archiv : an international journal of pathology》1995,427(1):105-110
Two cases of anaplastic large cell Ki-1 lymphoma involving bone as the most prominent and initial manifestation are reported. The first patient was a 20-year-old male who had back pain and incomplete paraparesis due to vertebral involvement. The second was a 14-year-old girl, whose first clinical signs were fever of unknown origin and sternal bone pain. Radiologically, skeletal lesions were lytic and destructive. Histopathologically, the tumour cells had pleomorphic bizarre nuclei and abundant basophilic cytoplasm. Immunohistochemically, Ki-1 (CD30) reactivity was strongly positive in both cases. Tumour cells were also CD3, CD4, epithelial membrane antigen and interleukin-2 receptor positive in the first case, and CD10, HLA-DR positive in the second case. The former tumour was considered to be of T-cell lineage and the latter of lymphoid progenitor cell origin. Radiation and chemotherapy were temporarily effective. However, both patients died 14 and 7 months after diagnosis, respectively, due to systemic lymph node involvement. These observations suggest that the prognosis for Ki-1 lymphoma involving bone is poorer than indicated in previous reports. 相似文献
974.
从人膀胱上皮癌细胞株T24的亚克隆株T24-8的无血清培养上清液经55%硫酸铵沉淀,再对无离子水透析,得到了有诱导LAK 细胞活性的粗提品。有此作用的因子称为LAK 细胞诱导因子(LAK-IF)。它的分子量为67KD,等电点为pH3.1~3.5。抗人IL-6单抗不能阻断LAK-IF 的作用。LAK-IF 粗提品经Mono-Q 柱快速蛋白液相层析见3个峰。第2峰蛋白质有LAK-IF 活性。 相似文献
975.
Safarova ER Shram SI Zolotarev YA Myasoedov NF 《Bulletin of experimental biology and medicine》2003,135(3):268-271
We studied the effects of Semax (antiinsulin peptide with neuroprotective effect) on the survival of cultured rat pheochromocytoma cell after oxidative stress induced by short-term incubation with hydrogen peroxide. Studies with fluorescent dyes propidium iodide and Hoechst 33258 showed that cell incubation with hydrogen peroxide led to the formation of damaged cells with characteristic signs of necrosis. Semax dose-dependently reduced the number of cells damaged by oxidative stress. The efficiency of Semax depended on the time of its addition to the culture medium. The results suggest that the neuroprotective effect of Semax in ischemic stroke can be due to its capacity to protect neurons from damage caused by oxidative stress. 相似文献
976.
Kvacheva ZB Shukanova NA Votyakov VI Lobanok ES Vorobei AV Nikolaeva SN 《Bulletin of experimental biology and medicine》2003,135(4):384-387
Membranotropic amphiphilic chromophore merocyanine 540 sensitized photodynamic inhibition of drug-resistant and sensitive variants of type I herpes simplex virus in cultured Vero cell. Optimal conditions of photodamage to virus particles and infected cells were determined (merocyanine 540 concentration 1 M, illumination dose 32.5-65.0 kJ/m2, exposure at early stages of infection). Infected cells actively bind the photosensitizer, which explains their selective photodamage. 相似文献
977.
Valrie Kouskoff Jean-Luc Vonesch Christophe Benoist Diane Mathis 《European journal of immunology》1995,25(1):54-58
The expression of recombination activating gene (RAG) products, responsible for T cell receptor (TcR) gene rearrangement, is shut off during positive selection of thymocytes. The precise stage at which this down-regulation occurs remains somewhat controversial. We have analyzed RAG-1 expression in thymocytes of TcR transgenic mice carried on selecting versus non-selecting genetic backgrounds, both by in situ hybridization on thymus sections and by polymerase chain reaction amplification of RNA from sorted cells. The data from several transgenic lines indicate that RAG expression is already reduced in immature, cortical, CD4+CD8+ cells in the presence of positively selecting major histocompatibility complex molecules, although complete shut-off is not achieved until the mature, medullary, single-positive stage. This finding has practical and theoretical significance for studies on the mechanism of positive selection. 相似文献
978.
本文采用 MTT 示踪法,检测极低频弱磁场对 SY 细胞有无影响。结果发现:频率固定为50Hz 时变化磁场幅值,在150uT、300uT 下细胞吸光度值有明显降低;幅值固定为150uT 时变化磁场频率,在25Hz、50Hz、75Hz 磁场照射下吸光度值均有不同程度下降,而在10Hz、60Hz、100Hz、300Hz、500Hz 磁场照射下没有变化;幅值固定为300uT 时变化磁场频率,在500Hz、75Hz、100Hz、300Hz、磁场照射下吸光度值均有不同程度下降,而在10Hz、25Hz、60Hz、500Hz 磁场照射下没有变化。 相似文献
979.
980.
对36例多发性骨髓瘤(MM)患者骨髓涂片,行酸性磷酸酶(ACP)染色。结果发现MM组的骨髓浆细胞ACP积分明显高于对照组。MM组初诊时、缓解期和复发期46人次检测结果表明,成MM病程中ACP积分病情变化而变化,缓解期的较初诊时降低,复发期的又复增高。这种变化有时较骨髓浆细胞比例的变化与病情的关系更为密切,提示ACP积分可作为MM病情监测的指标。 相似文献