全文获取类型
收费全文 | 1493篇 |
免费 | 69篇 |
国内免费 | 109篇 |
专业分类
儿科学 | 1篇 |
基础医学 | 83篇 |
口腔科学 | 477篇 |
临床医学 | 34篇 |
内科学 | 55篇 |
皮肤病学 | 51篇 |
神经病学 | 6篇 |
特种医学 | 13篇 |
外科学 | 23篇 |
综合类 | 215篇 |
预防医学 | 19篇 |
眼科学 | 3篇 |
药学 | 253篇 |
中国医学 | 436篇 |
肿瘤学 | 2篇 |
出版年
2024年 | 5篇 |
2023年 | 17篇 |
2022年 | 23篇 |
2021年 | 30篇 |
2020年 | 25篇 |
2019年 | 23篇 |
2018年 | 23篇 |
2017年 | 34篇 |
2016年 | 33篇 |
2015年 | 52篇 |
2014年 | 84篇 |
2013年 | 133篇 |
2012年 | 143篇 |
2011年 | 144篇 |
2010年 | 82篇 |
2009年 | 70篇 |
2008年 | 88篇 |
2007年 | 113篇 |
2006年 | 69篇 |
2005年 | 50篇 |
2004年 | 51篇 |
2003年 | 38篇 |
2002年 | 19篇 |
2001年 | 41篇 |
2000年 | 17篇 |
1999年 | 19篇 |
1998年 | 14篇 |
1997年 | 17篇 |
1996年 | 11篇 |
1995年 | 16篇 |
1994年 | 10篇 |
1993年 | 7篇 |
1992年 | 16篇 |
1991年 | 16篇 |
1990年 | 13篇 |
1989年 | 19篇 |
1988年 | 14篇 |
1987年 | 14篇 |
1986年 | 15篇 |
1985年 | 21篇 |
1984年 | 6篇 |
1983年 | 6篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1980年 | 7篇 |
1979年 | 7篇 |
1978年 | 3篇 |
1977年 | 1篇 |
1976年 | 6篇 |
1975年 | 3篇 |
排序方式: 共有1671条查询结果,搜索用时 15 毫秒
81.
目的:研究大孔吸附树脂分离纯化鱼腥草总黄酮工艺,为鱼腥草总黄酮的工业化生产提供实验依据。方法-以贵州产鱼腥草为原料,以鱼腥草总黄酮含量及回收率等为考察指标,选用大孔吸附树脂对鱼腥草总黄酮进行分离纯化,分别采用静态试验、动态试验等考察大孔树脂对鱼腥草总黄酮的分离纯化效果及影响因素。结果:AB-8型大孔吸附树脂对鱼腥草总黄酮静态饱和吸附量为76.20mg.g-1(干树脂),洗脱率97.35%,动态饱和吸附量为74.5mg.g-1(干树脂),总黄酮回收率在82.8%、纯度在80%以上,是实验树脂中分离纯化鱼腥草总黄酮的最佳大孔吸附树脂。结论:分离纯化鱼腥草总黄酮最佳工艺条件为:AB-8型大孔吸附树脂,洗脱剂为70%乙醇,洗脱剂用量为2~3倍树脂体积,上柱总黄酮量与树脂比为1:13.4,上柱液总黄酮浓度为17.57mg.mL-1,流速2-3m1.min-1,上柱液pH4-5。 相似文献
82.
妇科千金片薄膜包衣的工艺研究 总被引:4,自引:1,他引:4
实验研究了用聚丙烯酸树脂IV号为薄膜包衣成膜材料的包衣方法。结果表明:丙烯酸树脂IV号为成膜材料包制的薄膜衣片经与糖衣片平行比较,其抗湿性,抗热性,抗磨损均优于糖衣片,且降低了劳动强度,缩短了工作时间。 相似文献
83.
超声乳化白内障吸除人工晶状体植入术后远期人工晶状体混浊的研究 总被引:4,自引:1,他引:3
目的 分析超声乳化白内障吸除人工晶状体植入术后远期亲水性丙烯酸酯折叠式人工晶状体光学部发生混浊、引起视力障碍行人工晶状体置换术的原因。方法 对 19例 (2 2只眼 )超声乳化白内障吸除人工晶状体植入术后远期亲水性丙烯酸酯折叠式人工晶状体光学部发生混浊的患者 ,行人工晶状体置换术。将取出的 2 2片混浊人工晶状体进行裂隙灯和光学显微镜检查 ;应用钙特异性茜素红染色法和vonKossa嗜银染色法进行病理学检查 ;使用扫描电镜观察沉淀物的位置和形态 ;应用能谱分析法检查沉淀物中的元素成分。以透明人工晶状体作为对照。结果 在人工晶状体光学部表面和表面下可见沉淀结晶呈多种形态的不规则颗粒 ,其中光学部中央着色颗粒密集且粗大 ,周边着色颗粒稀疏而细小。这些颗粒茜素红染色和vonKossa嗜银染色均为阳性反应。能谱分析证实颗粒中含钙和磷元素。人工晶状体襻和透明人工晶状体均无相同表现。结论 超声乳化白内障吸除人工晶状体植入术后远期 ,亲水性丙烯酸酯折叠式人工晶状体混浊的原因 ,为人工晶状体光学部表面和表面下发生钙和磷化合物沉淀结晶 相似文献
84.
目的筛选分离葛根总黄酮的最佳树脂,并对影响分离的各种因素进行系统的研究,使纯化工艺达到最优。方法采用静态与动态的吸附-解吸两种方法,利用紫外可见分光光度计测量葛根总黄酮的含量,研究不同大孔吸附树脂及其不同的工艺条件对总黄酮分离纯化的影响。结果 SP70分离效果最好,其最佳工艺为药液浓度0.5 g·mL-1(相当于原生药)、pH为5 -6、以2 BV·h-1速率进行上样,上样量为60 BV,以5 BV的70%乙醇、2 BV·h-1的流速进行洗脱,效果最佳。经SP70处理后的葛根总黄酮的含量可达80%以上。结论大孔吸附树脂SP70分离纯化总黄酮效果较好,适合工业生产。 相似文献
85.
活性氧化铝除氟剂的除氟性能研究 总被引:10,自引:0,他引:10
目的通过对经特殊工艺处理的不同粒径的活性氧化铝的除氟性能进行研究.开发实用的除氟剂。方法模拟A(粒径2.0~3.0mm)、B(粒件3.5~5.5mm)样除氟剂对氟含量为10mg/L的原水进行处理试验,研究除氟性能。结果A、B样除氟剂当流速分别为80ml/min及以下和20ml/min及以下时,其终端流出水中的氟含量均小于1mg/L;A、B样除氟剂的绝对饱和吸附最分别为1.67mg/g和1.30mg/g,相对饱和吸附量分别为1.57mg/g和0.17mg/g。结论除氟剂粒径小、比表面积较大时,有利于除氟性能的提高。 相似文献
86.
大孔吸附树脂分离纯化荷叶中阿朴啡类生物碱 总被引:3,自引:0,他引:3
目的优化大孔吸附树脂分离纯化荷叶中阿朴啡类生物碱的洗脱条件。方法采用大孔吸附树脂柱色谱法,以不同体积分数甲醇水溶液进行洗脱,分离富集阿朴啡类生物碱,并配以HPLC进行同步监控。结果基于不同体积分数甲醇水洗脱液对各种阿朴啡类生物碱洗脱能力不同,采用70%、80%、95%甲醇水溶液进行梯度洗脱,荷叶中3种主要的阿朴啡类生物碱得到了分离富集。70%甲醇水溶液洗去目标物以外的杂质后,75.58%的N-降荷叶碱和65.03%的O-降荷叶碱富集于80%甲醇水洗脱液中,69.46%荷叶碱富集于95%甲醇水洗脱液中。各洗脱液分别蒸干后得固体,N-降荷叶碱和O-降荷叶碱在相应的固体中质量分数分别为44.01%、7.61%。荷叶碱在相应的固体中质量分数为68.52%。结论此方法操作简单、重复性好,能有效分离富集荷叶中阿朴啡类各种生物碱。 相似文献
87.
大孔吸附树脂分离肿节风中总黄酮的研究 总被引:11,自引:0,他引:11
目的考察11种大孔吸附树脂对肿节风总黄酮的吸附分离性能。方法采用静态吸附分离法确定适合的大孔吸附树脂;采用动态吸附分离法确定分离条件。以总黄酮吸附量、总黄酮质量分数和回收率为考察指标,采用紫外分光光度法测定总黄酮。结果HPD 400大孔吸附树脂对肿节风总黄酮有良好的吸附分离性能,其分离肿节风总黄酮的工艺条件为:肿节风总黄酮上样质量浓度为10 m g/mL,肿节风总黄酮最大吸附量为9.5 m g/mL,吸附体积流量为2.5 mL/m in,洗脱剂为70%乙醇,洗脱剂用量为3倍柱体积,树脂可重复使用3次。结论采用HPD 400大孔吸附树脂吸附分离肿节风总黄酮简便有效,总黄酮回收率为85%左右。 相似文献
88.
Does chemomechanical caries removal affect dentine adhesion? 总被引:2,自引:0,他引:2
The purpose of this study was to determine whether shear bond strengths (SBS) to chemomechanically excavated dentine (Carisolv) differed from the SBS after conventional caries removal (bur). The following adhesive systems were used: Dyract AP/NRC/Prime & Bond NT; Dyract AP/Prime & Bond NT; Tetric Ceram/ Syntac SC; Tetric Ceram/Syntac SC (no etch) (TC 2); Definite/Etch & Prime 3.0; Definite/experimental. One-hundred and twenty human molars with occlusal caries were assigned to 12 groups. Sequential caries removal was controlled with laser fluorescence. After water storage, the samples were tested in a single-plane shear test assembly. The results demonstrated differences between the bonding systems, whereas the mode of caries removal had no consistent effect. If total etch technique was neglected, the results of the composite resin (TC 2) indicated a tendency to higher SBS in the Carisolv group (18.6 +/- 4.6 MPa) compared with conventional treatment (14.1 +/- 3.9 MPa). The present data demonstrate that chemomechanical caries removal has no adverse effect on bonding of modern adhesive systems to dentine. Smear layer-dissolving or -modifying bonding systems could potentially benefit from chemomechanical pretreatment. 相似文献
89.
Previous work has presented a study of the mechanical properties of denture base resins reinforced with a new type of high performance fibre. It is now shown that the substantial improvements demonstrated in those composites remain largely unaffected by a watery environment, anatomical notches, moulding pressure and other factors of denture construction. The understanding of the reinforced resins is here complemented with a detailed study of the interface strength, taking into account the various couplings occurring within the system. 相似文献
90.
Mitsuru Suzuki Shingo Yamane Takao Matsugane Takuo Nobuto Nakanobu Azuma Toshio Nishideu Masaki Shinomiyat† Kou Saito† Norihiro Sasakind‡ Yukihiko Nose§ 《Artificial organs》1996,20(4):296-302
Abstract: A comparative assessment has been made regarding efficacy and safety of the double filtration plasmapheresis (DFPP), thermofiltration (TFPP), and low–density lipoprotein (LDL) adsorptive (PA) methods by making a crossover test on heterozygous familial hypercholesterolemia patients. Treatments by DFPP, TFPP (secondary membrane Evalux 5A), and PA (Liposorber LA–40) were carried out 5 times each, with a 2–week interval, in 5 patients with heterozygous familial hypercholesterolemia. The same plasma separator (Plasmacure PS–60, polysulfone) was used in all cases, and the volume of plasma processed was set at 4 L. High removal rates were obtained of total cholesterol, LDL cholesterol, triglycerides TG, and apolipoprotein B (apoB) by all three methods, and no differences were observed. Lipoprotein (a), apoA–2, apoC–3, fibrinogen, and immunoglobulin M (IgM) showed significantly high removal rates by the DFPP and TFPP methods compared with the PA method.
The sieving coefficient of albumin and high–density lipoprotein (HDL) cholesterol at 2 and 4 L of plasma processed exhibited high permeabilities using all three methods. Supplementing albumin was not necessary. An increase of the transmembrane pressure was observed in 1 case treated by DFPP but was not observed when using the TFPP or PA method. No changes were observed in serum interleukin 1β (IL–lβ) or tumor necrosis factor–a (TNF–α) before and after treatment by any of the three methods. No remarkable side effects were observed using either the DFPP or TFPP method. The DFPP and TFPP methods showed efficacy and safety that was not inferior to the PA method in conventional LDL apheresis, and the dead–end method of the filter operation without the discarding of plasma was shown to be possible. 相似文献
The sieving coefficient of albumin and high–density lipoprotein (HDL) cholesterol at 2 and 4 L of plasma processed exhibited high permeabilities using all three methods. Supplementing albumin was not necessary. An increase of the transmembrane pressure was observed in 1 case treated by DFPP but was not observed when using the TFPP or PA method. No changes were observed in serum interleukin 1β (IL–lβ) or tumor necrosis factor–a (TNF–α) before and after treatment by any of the three methods. No remarkable side effects were observed using either the DFPP or TFPP method. The DFPP and TFPP methods showed efficacy and safety that was not inferior to the PA method in conventional LDL apheresis, and the dead–end method of the filter operation without the discarding of plasma was shown to be possible. 相似文献