122例急性戊型肝炎重叠感染患者的肝功能及临床特点分析

目的 比较分析单纯急性戊型肝炎与重叠感染患者的肝功能指标和临床特点.方法 以40例单纯急性戊型肝炎为对照,回顾性分析了122例重叠感染戊型肝炎患者的肝功能指标、临床资料.结果 单纯戊肝组、甲戊重叠感染组、乙戊重叠感染组,三组之间ALT、AST、TBIL、DBIL差异均无统计学意义(P0.05),急性戊肝组与乙戊重叠感染组,甲戊重叠感染组与乙戊重叠感染组的白蛋白、球蛋白有统计学差异(P<0.01);甲戊重叠感染、乙戊重叠感染与单纯戊肝相比较临床合并症多、并发症重.结论 戊型肝炎重叠感染,特别是乙戊重叠感染患者,要加强临床监测,提高早期预防并发症的意识,以降低病死率.     

阿尔茨海默氏病和帕金森氏病对老年人认知功能及日常生活能力的影响分析

目的探讨阿尔茨海默氏病(AD)和帕金森氏病(PD)对老年人认知功能及日常生活能力的影响。方法对2000～2003年就诊于我院的53例AD、68例PD和60例正常人应用简易智力量表(MMSE)和日常生活能力量表(ADL)进行分析。结果AD组与对照组相比MMSE总分及各因子分、ADL总分及各单项分均有显著性差异(P<0.01):PD组与对照组相比除MMSE的记忆力和语言因子分以及ADL第13项目(打电话)外,其余均有显著性差异(P<0.05):AD组与PD组相比除ADL第7项(穿衣)外均有显著性差异(P<0.05)。结论AD和PD对老年人的认知功能和日常生活能力均有显著的影响,两者相比,AD对老年人的影响更严重、更广泛。     

Vascular anatomy of gracilis muscle arterial findings to enhance graciloplasty

Dynamic graciloplasty has recently been developed for reconstruction of anal function in patients who are fecally incontinent in preference to permanent abdominal colostomy. Since the muscular portion of gracilis is wrapped around the neoanus, the length of the gracilis arc influences the functional outcome of graciloplasty. Although dissection of the main pedicle (i.e. the main artery and vein) can facilitate gracilis to have enough muscle arc, it has been unclear whether there are any vessels proximal to the main pedicle or through the origin of the muscle which could support blood flow into the whole of gracilis. In this study, the vascular anatomy of gracilis in both legs of 26 Japanese cadavers was examined. All muscles had a main pedicle, mean maximum diameter 1.08 mm, entering at the proximal one-third of the muscle. However, only 18 muscles (34.6%) had an accessory artery in the proximal portion in addition to the main pedicle. Some arteries always exist at the origin of the muscle, having a mean maximum diameter of 0.34 mm, suggesting that they might be able to support the whole gracilis without supply from the main pedicle.     

Cell cholesterol esters and high-density lipoprotein plasma levels during liver hyperplasia in choline-fed male and female rats

Sexual dimorphism exists in the response of rats to lead nitrate, liver hyperplasia occuring earlier and being more pronounced in males. Excess dietary choline in females shifted the growth pattern towards that of males. To determine whether phosphatidylcholine-induced growth modulations could be related to a derangement of cholesterol metabolism, liver accumulation of cholesterol esters and plasma lipoprotein patterns were investigated. In males, lead-induced liver hyperplasia was associated with increased total cholesterol hepatic content, accumulated cholesterol esters and reduced concentration of plasma High Density Lipoprotein (HDL) cholesterol. Females were less responsive to the liver mitogenic signal of lead nitrate; there was no elevation of cholesterol content nor any marked accumulation of cholesterol esters. This is consistent with the lack of change in the plasma levels of HDL cholesterol. Continuous choline feeding displaced the liver cholesterol ester pattern and plasma HDL cholesterol levels in females, and in parallel that of DNA synthesis, towards those of males. Choline was not observed to have any effect in males. These results suggest that the derangement of phosphatidylcholine metabolism induces growth-related changes in cholesterol turnover; they are consistent with the proposal that the intracellular content of cholesterol esters may have a role in regulating liver growth rates.     

血小板活化因子受体拮抗剂的抗内毒素肝损伤作用研究

本文研究了血小板活化因子受体拮抗剂ＢＮ５２０２１及ＢＮ５０７３９的抗大鼠内毒素肝损伤的作用。结果表明ＢＮ５２０２１及ＢＮ５０７３９能使内毒素所致的肝线粒体膜磷脂降解及溶血磷脂的聚集，脂质过氧化反应的增强以及机体抗氧化能力的降低得到明显改善，提示ＢＮ５２０２１及ＢＮ５０７３９是两种强有效的抗内毒素肝损伤的药物，用于内毒素肝损伤的治疗。     

脾切除对门脉高压症大鼠CD4、CD8和脾功能的影响

目的:研究肝硬化门脉高压症（PHT）大鼠脾次全切除术后CD4、CD8与脾功能的变化。 方法: 皮下注射60％四氯化碳复制大鼠肝硬化模型。共分5组：正常大鼠组、肝硬化（PHT）组、正常大鼠脾切除组、PHT全脾切除组、PHT脾次全切除组，每组10只。手术后第4周检测各组血常规、肝功能、tuftsin、CD4、CD8等指标。 结果:PHT高压症大鼠脾切除术后tuftsin水平［（171±21）ng/L vs （433±44） ng/L,P<0.01］、CD4/CD8（2.01±0.22 vs 1.12±0.12, P<0.01)均显著低于PHT组;PHT大鼠脾次全切除组tuftsin［（434±42）ng/L vs （171±21）ng/L, P<0.01］、CD4/CD8 （1.97±0.18 vs 1.12±0.12, P<0.01)显著高于PHT大鼠脾切除组。肝功能两者无显著差异（P>0.05）。 结论: PHT脾次全切组大鼠术后免疫和脾功能比PHT脾全切组大鼠明显改善，肝功能两者无显著变化。     

Regional left ventricular function in the three main coronary artery territories at rest and during exercise

Summary Twenty consecutive patients (mean age 51.6 years) with persistent severe angina pectoris underwent aorto-coronary bypass surgery receiving an overall of 60 anastomosis. On an average, 9.4±1.5 months p.o. first pass radionuclide ventriculograms (18 to 24 mCi 99 m Technetium-Pertechnetate i.v.) were performed at rest and after excerise. Besides measurement of global ejection fraction (GEF), regional ejection fraction (REF) was assessed employing for the first time a new technique: each RAO-view of p.o. radionuclide left ventriculogram was subdivided into three regions according to supply of the three main coronary arteries and their branches as visualized on pre-operative coronary angiogram.GEF improved after maximum exercise in 13 cases by 8.1% points (from 50.4 to 58.5%), remained unchanged three times and decreased four times by 7.1 points (from 51.6 to 44.5%; all changesp<0.05).In completely revascularized regions (n=35) REF improved 24 times by 9.7 points (from 51.1 to 60.8%), did not differ from rest REF six times and decreased in three case by 7.3 points (from 48.6 to 41.3%; all changesp<0.05). Completely revascularized regions responded to exercise like normally perfused areas (increase 7.8 points (from 50.6 to 58.4%;n=7;p<0.05).REF deteriorated in incompletely revascularized regions (n=9) six times by 12.8 points (from 58.0 to 45.2%), remained unchanged twice and improved once by 4.5 points. Total group's REF decreased by 7.3 points (from 56.8 to 49.5%;p<0.05). Exercise REF of incompletely revascularized regions was highly significant inferior to that of completely revascularized regions (49.5 to 58.4%;p<0.01).GEF is a weighted balance of the three regional ejection fractions. The most important parameter is REF of LAD territory.     

工作记忆、执行功能、加工速度与数学障碍儿童推理和心算能力的关系

目的：探讨工作记忆、执行功能、加工速度与数学障碍儿童推理和心算能力的关系。方法：采用智力测验、成就测验、教师提名、学业成绩相结合的综合标准甑选数学障碍儿童，在长沙市三所小学随机选取4-6年级共12个班，从中抽取24名数学障碍儿壹和24名学习正常儿童。对两组儿童进行工作记忆、执行功能、加工速度以及数字推理、图案推理和心算测验。结果：数学障碍组的工作记忆、执行功能及加工速度测验成绩均屁著低于学习正常组（P〈0．001—0．05），数学障碍组数字推理、图案推理和心算成绩也湿著低于正常组；数字推理、图案推理及心算成绩与听觉工作记忆、视觉工作记忆、执行功能和加工速度均有显著相关；逐步回归分析结果显示，进入推理能力回归方程的自变量有听觉工作记忆和视觉工作记忆；进入心算的回归方程的是听觉工作记忆和加工速度。结论：数学障碍儿童存在工作记忆、执行功能和加工速度缺陷；数学障碍儿童的推理能力低于正常学习儿童，主要可由工作记忆能力不足来解释，而心算能力低下则可由听觉工作记忆和加工速度共同解释。     

PTEN基因对人肝癌细胞系HHCC作用的研究

目的：研究PTEN基因对人肝癌细胞系HHCC细胞恶性表型及凋亡的影响。方法：将PTEN真核表达载体pBabe-PuroPTEN及空质粒pBabe-Puro，通过脂质体介导的基因转染方法，转入该基因表达缺失的HHCC细胞系中，嘌呤霉素筛选获得稳定表达PTEN基因的转染细胞。通过平板克隆形成试验、DNA凝胶电泳、相差显微镜和电镜，观察PTEN基因对人肝癌细胞系HHCC恶性表型及凋亡的影响。结果：转染后的细胞，经原位杂交、免疫组织化学检测证实，有PTEN mRNA及其蛋白的表达；平板克隆形成试验证实，转染PTEN基因后，细胞形成克隆的能力降低；转染PTEN的细胞在相差显微镜和电镜下可出现典型的凋亡形态学改变；DNA琼脂糖凝胶电泳呈现出明显的梯状条带。结论：外源性PTEN基因导入HHCC细胞后，可降低HHCC的细胞的恶性表型，并促进凋亡发生。