白茶、玫瑰花及玳玳花提取物对体外培养黑素细胞功能的影响

[目的]观察白茶、玫瑰花及玳玳花提取物（祛斑因子A、B）对体外培养的正常人表皮黑素细胞功能及角质形成细胞和黑素细胞共培养体系中黑素小体转运的影响。[方法]制备含药血清,建立正常人表皮黑素细胞体外培养体系,采用4-甲基偶氮唑蓝（MTT）法测定黑素细胞增殖,酪氨酸酶多巴速率氧化法测定酪氨酸酶活性,NaOH裂解法测定黑素生成量,逆转录-聚合酶链反应（RT-PCR）检测黑素细胞酪氨酸酶mRNA的表达,Pull-down法检测黑素细胞GTP-RhoA和GTP-Rac1蛋白的表达;建立正常人角质形成细胞和黑素细胞的体外共培养体系,流式细胞术检测共培养细胞体系中黑素转运的情况。[结果]与空白血清对照组相比,祛斑因子A、B可显著抑制酪氨酸酶活性;祛斑因子A可显著降低酪氨酸酶mRNA的表达。祛斑因子A中、低剂量可抑制树突结构蛋白的表达。祛斑因子A高、中、低剂量及祛斑因子B均可抑制黑素小体的转运。[结论]为祛斑因子用于色素性疾病的保健防治提供一定的实验依据。     

角质细胞生长因子及其受体在葡萄胎恶性变中的作用

目的探讨角质细胞生长因子(KGF)及其受体(KGFR)在葡萄胎组织发生、发展及预后中的意义和作用。方法收集葡萄胎、侵蚀性葡萄胎、绒癌组织,采用免疫组化、原位杂交法分别从蛋白质、mRNA水平检测KGF和KGFR在良恶性葡萄胎组织中的表达,染色强度及阳性细胞数进行统计分析。结果良性葡萄胎组织滋养细胞中KGF和KGFRmRNA、蛋白均有一定程度表达,定位于细胞滋养细胞、合体滋养细胞胞质。恶性葡萄胎组织KGF表达与良性葡萄胎无差异,但KGFR在合体滋养细胞中表达明显高于良性葡萄胎,两者之间有显著性差异(χ2=12·775,P<0·01)。KGFR在侵蚀性葡萄胎及绒癌组织中的表达也明显高于良性葡萄胎(χ2=19·542,χ2=24·723,P<0·001)。结论KGF及KGFR的表达与滋养细胞肿瘤转移密切相关,在葡萄胎恶性变及浸润、转移中起重要作用。     

角质形成细胞源促成纤维细胞增殖的细胞因子研究

目的:研究正常角质形成细胞分泌的促进成纤维细胞增殖的细胞因子。方法:采用免疫组化法测定角质形成细胞所合成分泌的TGF-β1,IL-1α,分别单独及联合应用TGF-β1抗体I、L-1α抗体中和角质形成细胞条件培养基,以Cellcountingkit-8测定中和前后其对成纤维细胞增殖的影响。结果:正常角质形成细胞可合成分泌大量TGF-β1,IL-1α,抗体中和前后角质形成细胞条件培养基对成纤维细胞的增殖作用有明显差异。结论:正常角质形成细胞分泌TGF-β1,IL-1α及一些未知的活性因子,可促进成纤维细胞增殖。     

A study on a chitosan-gelatin-hyaluronic acid scaffold as artificial skin in vitro and its tissue engineering applications

Chitosan-gelatin-hyaluronic acid scaffolds for tissue regeneration were fabricated by freezing and lyophilizing methods. The scaffolds showed a higher water uptake and retention abilities than chitosan-gelatin scaffolds did. Fibroblasts cultured in chitosan-gelatin-hyaluronic acid scaffolds grew and proliferated well, and they exhibited a strong viability. Keratinocytes were co-cultured with fibroblasts in chitosan-gelatin-hyaluronic acid scaffolds to construct an artificial bilayer skin in vitro . The artificial skin obtained was flexible and had good mechanical properties. The data from this study suggested that chitosan-gelatin-hyaluronic acid scaffolds are suitable for preparing a bilayer skin substitute.     

Altered microRNA expression profile with miR-27b down-regulation correlated with disease activity of oral lichen planus

Oral Diseases (2012) 18 , 265–270 Background: Increasing evidence indicates that microRNAs (miRNAs) play a vital role in the pathogenesis of inflammatory and autoimmune diseases. The objective of this study was to investigate the altered miRNA expression profile in patients with oral lichen planus (OLP) and determine the miR‐27b expression. Methods: We compared miRNA expression patterns in oral biopsy specimens from patients with OLP (n = 3) with those from normal controls (n = 3) using microarray technology. We further assessed the miR‐27b expression in specimens from patients with OLP (n = 53) against controls (n = 34) using real‐time quantitative PCR (RT‐QPCR), and miR‐27b expression in specimens from patients with OLP (n = 15) against controls (n = 12) using in situ hybridization (ISH). Results: Using microarray analysis, a total of 46 differentially expressed miRNAs with more than 2‐fold change were identified, including 8 up‐regulated and 38 down‐regulated miRNAs. Both RT‐QPCR and ISH analyses revealed that miR‐27b was significantly down‐regulated in OLP tissue, and miR‐27b expression was even more suppressed in atrophic‐erosive OLP than in reticular OLP. In addition, miR‐27b was found to be expressed in the epithelial keratinocyte layer of both normal and OLP tissues. Conclusion: These data indicate that miRNAs may be the novel candidate biomarkers for the implication of miRNAs in the pathogenesis of OLP.