盼生安对BEL-7402肝癌细胞的诱导分化作用

目的研究盼生安对BEL-7402肝癌细胞的诱导分化作用。方法应用细胞生物学的方法观察盼生安对细胞生长特性的影响；形态计量学方法研究对细胞、细胞核、核浆比及AgSORs形态计量学参数的影响；流式细胞术测定细胞周期；常规生化测定对ALP和LDH酶活力的影响；RNA点印记杂交测定对c-N-ras癌基因表达的影响。结果经盼生安处理后，可使BEL-7402肝癌细胞生长速度显著减慢。细胞及细胞核的面积、等效直径、等效体积、核浆比均随着剂量的增大而减小．同时AgNORs颗粒和面积明显减少。细胞周期出现向G0／G1期移行的特征动力学改变。两种肝癌标志酶ALP及LDH活性降低，且有较好的剂量．效应关系。c-N-ras癌基因表达受到抑制。结论初步表明盼生安具有诱导BEL-7402肝癌细胞分化的作用。     

诱导白木香产生沉香真菌的固体培养条件优化

 目的 对诱导白木香产生沉香的3株活性真菌的固体培养条件进行优化,为该类真菌的推广应用提供参考。方法 采用真菌固体培养方法,对影响真菌生长的营养和培养条件等因子进行优化,实验结果采用单因素方差分析法。结果 得到了培养3株活性真菌的最佳固体培养基配方,获得了真菌固体生长的最适温度、pH值、装料量、基质含水量和光照条件。结论 在实验得到的最佳条件下,能够较快的培养出高质量的固体菌种;为3株活性真菌诱导白木香产生沉香的生产推广提供了菌种培养的优化条件。     

Recombinant gamma interferon induces HLA-DR expression on squamous cell carcinoma,trichilemmoma, adenocarcinoma cell lines,and cultured human keratinocytes

Summary We investigated the effects of recombinant human gamma interferon on the induction of HLA-DR expression by two human squamous cell carcinoma, three trichilemmoma, one eccrine carcinoma, two adenocarcinoma cell lines, and cultured human keratinocytes in vitro. None of eight epithelial cell lines or keratinocytes expressed HLA-DR without gamma interferon treatment. In contrast, pure gamma interferon (500 IU/ml, 72-h treatment) induced HLA-DR expression on 1/2 squamous cell carcinoma, 3/3 trichilemmoma, 2/2 adenocarcinoma cell lines, and 4/4 kerationcyte cell lines, as determined using a fluorescence-activated cell sorter. A maxillary squamous cell carcinoma line and an eccrine carcinoma cell line failed to express HLA-DR with gamma interferon treatment; however, the growth of cells was inhibited by gamma interferon treatment. By indirect immunoperoxidase techniques, tumor cells such as Bowen's disease and squamous cell carcinoma were found to express HLA-DR. Since HLA-DR expression has been shown to be important for various immune responses, these findings suggest that gamma interferon plays important roles in various immune-related skin diseases.     

Patient-Perceived Pressure from Clinicians for Labor Induction and Cesarean Delivery: A Population-Based Survey of U.S. Women

ObjectiveTo determine whether patient-perceived pressure from clinicians for labor induction or cesarean delivery is significantly associated with having these procedures.ConclusionsPatient-perceived pressure from clinicians significantly predicts labor induction and cesarean delivery. Efforts to reduce provider–patient miscommunication and minimize potentially unnecessary procedures may be warranted.     

Respiratory failure during induction chemotherapy for acute myelomonocytic leukaemia (FAB M4Eo) with ara-C and all-trans retinoic acid

We report two cases of acute myeloid leukaemia FAB classification M4Eo with high white cell counts at presentation, who developed acute respiratory failure with pulmonary infiltrates on chest radiograph soon after commencing conventional cytotoxic chemotherapy plus all-trans retinoic acid (ATRA). We suggest that in patients with M4Eo ATRA should be used with caution, perhaps delaying its commencement until the white cell count is < 10 x 109/l.     

DAPTO等方案诱导治疗急性双表型白血病11例报告

报告１１例急性双表型白血病的诱导化疗结果。５例ＡＢＬ经ＤＡＰＴＯ方案诱导化疗，４例于２８－５０天内完全缓解，持续ＣＲ时间７－１４月，存活时间８－１８月，１例仍无病生存，其余６例应用ＤＶＰ等方案化疗，ＣＲ２例，持续ＣＲ分别为４和８月，６例患者作１例生存１６月外，余未超过７月。