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91.
从大鼠肝分离出小分子蛋白质,注入肝大部切除的大鼠体内。术后24h制备肝细胞悬液及骨髓细胞悬液,用流式细胞计测定细胞DNA含量。结果:①实验组大鼠每克体重注入肝提取物10~300μg,一致呈现4n肝细胞DNA复制受阻于G_1期,各类肝细胞数与正常大鼠的相似;②对照组大鼠,4n肝细胞由正常的68.1%降至30.9%,8n肝细胞由6.3%骤增至25.3%,并出现16.8%的4n~8n间8期肝细胞;③两组大鼠的骨髓细胞DNA含量均无变化。表明肝提取物中含有肝抑素样物质,它特异性地使再生肝的4n肝细胞增殖周期阻滞于G_1期及S早期。 相似文献
92.
Antonio Giorgio M.D. Luciano Tarantino Giampiero Francica Nicola Mariniello Antonio Nuzzo Luca del Viscovo Antonio Rotondo 《Cardiovascular and interventional radiology》1996,19(1):27-31
Purpose: To verify the efficacy of ultrasound (US)-guided injection of large amounts of ethanol into large or multiple liver lesions,
in a single session under general anesthesia (one-shot PEI) for percutaneous ablation of hepatic tumors.
Methods: Twenty-nine patients (27 with 51 hepatocellular carcinoma (HCC) nodules on cirrhosis, diameter range 1.0<+>–<+>9.0 cm; two
patients with a single metastasis from the gastroenteric tract, 5.0 and 9.0 cm, respectively, in diameter) were treated with
one-shot PEI.
Results: The total volume of alcohol delivered per patient ranged from 16 to 210 ml. Mean ethanol volume in all patients was 49 ml.
Dynamic computed tomography (CT) examination showed complete necrosis in 41 of 50 lesions. Two patients died of hypovolemic
shock due to massive upper gastrointestinal bleeding, 3 and 7 days, respectively, after the interventional procedure. All
the remaining patients are alive (follow-up 5<+>–<+>14 months) except one who died of liver failure 5 months after. New HCC
nodules occurred in six patients within 6 months and one intralesional relapse was recorded.
Conclusion: In this preliminary experience, one-shot PEI is as effective in inducing liver tumor necrosis as traditional PEI; its advantages
are shorter treatment time and the capability of treating larger and multiple liver lesions. 相似文献
93.
本文报告50例肝硬化患者SCG含量的临床意义.结果表明,SCG测定诊断肝硬化的阳性率为92%,明显高于SGPT(53%)及ADA(78.6%)的阳性率.Child-pugh肝功能分级中A、B、C级SCG值分别为10.14±6.03,23.55±11.27和54.66±24.47μmol/L(P<0.01).相关分析表明,SCG与血清白蛋白含量呈负相关(r=-0.5018.P<0.01),与胆红素含量呈正相关(r=0.6964,P<0.01).并发肝性脑病和原发性肝癌者及死亡病例,其SCG升高更为明显.我们认为肝硬化患者SCG值大于40μmol/L预示病情危重,预后不良. 相似文献
94.
目的 观察HO CO系统在肝硬化病人肝组织中的表达及与门静脉压力的关系 ,以探讨其在肝硬化门脉高压中的作用。方法 随机选取 2 0例正常志愿者及 2 0例肝硬化患者 ,在B超引导下经皮经肝穿刺分别测定门静脉压力、抽取门静脉血和外周血并留取肝组织 ,测定血液中CO浓度 ,用免疫组化和RT PCR方法观察肝组织HO 1及其HO 1mRNA的表达。结果 肝硬化病人下腔静脉及门静脉血中CO浓度、肝组织HO 1、HO 1mRNA的表达及门静脉压力均分别显著高于正常对照组 ,正常对照组的外周血和门静脉血中CO浓度水平接近 ,无明显差异 ;但肝硬化患者的门静脉血CO浓度显著高于外周血CO浓度。结论 门脉血CO浓度、肝组织中HO 1以及HO 1mRNA表达与门静脉压力密切相关 相似文献
95.
肝钳与肝门阻断的肝损害及其防治的实验研究 总被引:2,自引:0,他引:2
本实验观察不同阻断时刻肝钳与肝门阻断的肝损害以及预防性应用抗氧化剂的缓解作用。结果表明:(1)SGPT、TB在肝钳组的变化比肝门阻断组表现得更为一过性。而肝门阻断给药组的改变要比非给药组的轻微且恢复得快。(2)肝门阻断时,MDA含量显著升高,机体的T-AOC和SOD下降。而给药组可明显减轻上述改变。(3)随着肝门阻断时间的延长,肝组织损害也相应加重,抗氧化剂的给予缓解了肝缺血和再灌注造成的组织损害。肝钳组由于健肝血供未受干扰,因此,不同阻断时刻肝组织学形态与对照组相似无甚改变。结论:(1)肝钳较肝门阻断的肝损害轻,且实用安全。(2)肝门阻断预防性使用抗氧化剂,可明显减轻肝缺血再灌注损害。(3)肝门阻断时经门静脉较外周静脉输注抗氧化剂,其保护作用更直接、明显 相似文献
96.
The Effects of Serum from Patients with Acute Liver Failure on the Growth and Metabolism of Hep G2 Cells 总被引:6,自引:0,他引:6
In many bioartificial liver systems currently being designed and evaluated for use in fulminant hepatic failure, direct contact is required between the patient's blood and the liver cells in the device. The efficacy of such devices will be influenced by the interaction of fulminant hepatic failure (FHF) patient serum with the cells. We have found that FHF serum inhibits the growth rate and the synthesis of DNA, RNA, and protein; disturbs glutathione homeostasis; and induces morphological changes in cultured human Hep G2 cells. These interactions should influence the design of bioartificial liver devices based on proliferating cell lines and indicate the requirement to pretreat FHF patient plasma to reduce the toxin load. 相似文献
97.
Peripheral blood CD3 and CD4 T-lymphocyte reduction correlates with severity of liver cirrhosis 总被引:2,自引:0,他引:2
L. Lombardo A. Capaldi G. Poccardi P. Vineis 《International Journal of Clinical & Laboratory Research》1995,25(3):153-156
Summary Immunological disturbances with impairment of immune function and a higher incidence of lymphoproliferative disorders and
other malignancies have been described in liver cirrhosis patients. To investigate the pathogenetic mechanism(s) involved
in such associated we looked for a possible imbalance in peripheral blood T-lymphocyte subpopulations in patients with liver
cirrhosis of differing severity. Immunophenotyping and counts of peripheral blood T-lymphocyte subpopulations were carried
out using monoclonal antibodies conjugated with different fluorochromes in 31 consecutive cirrhotic patients and 23 matched
healthy volunteers. Univariate and multivariate analyses of lymphocyte phenotype counts were performed and odds ratios were
computed. Statistically significant associations, according to both univariate and multivariate analyses, were found between
case/control status and mean CD3 and CD4 T-lymphocyte counts (P<0.0001). A strong correlation was found between the Pugh’s index and CD3 and CD4 lymphocyte counts, with a clear reduction
of these phenotypes with increasing liver cirrhosis. Median CD3 and CD4 values were 2,283 and 1,329/μl respectively among
controls and 896, 801, and 492/μl and 515, 514, and 307/μl, respectively in categories A, B, and C of Pugh’s classification.
Very high odds ratios were found using the median values of CD3 and CD4 as a threshold. There was a statistically significant
decrease for each of the T-cell phenotypes studied (CD2, CD3, CD4, CD8, CD16, CD19, CD20, CD56, CD57) between patients and
controls (P<0.0001). The progressive and severity-related decrease in mean peripheral blood CD3 and CD4 counts in liver cirrhosis suggests
a progressive impairment of protective immune function and may be a factor facilitating malignancy in cirrhotic patients. 相似文献
98.
大鼠颌下腺切除对CCl4所致肝损伤修复的影响 总被引:1,自引:0,他引:1
实验大鼠分为颌下切除+CCl4组,假手术+CCl4对照组及正常对照组,结果假手术+CCl4第2d时Ⅲ带细胞轻度受损,第4d、8d时受损面积略大,第12d时大部分细胞恢复,而颌下腺切除+CCl4组则损伤明显,第8d时胞质极度稀疏,12d时大部分细胞尚未恢复,与假手术组比较,该组Feulgen反应略弱,PAS反应减弱,第4~8d时较明显,G-6-P,SDH活性均减弱。结果表明颌下腺切除导致内源性表皮生 相似文献
99.
Hiroshi Shimada Masao Nanko Shoichi Fujii Hidenobu Masui Shinji Togo Hideyuki Ike Akira Nakano Shigeo Ohki 《Journal of Hepato-Biliary-Pancreatic Surgery》1995,2(2):116-121
Hepatic micrometastases of the parenchyma adjacent to a macroscopic lesion were detected in 17 of 31 resected liver metastases.
Fifty-nine micrometastatic lesions were detected in total; 26 lesions were situated in the portal vein (PV), 22 in the central
vein (CV), 5 in the bile duct (BD), and 6 in the sinusoid (SS). A histological study confirmed the direct invasion of the
macrometastatic cancer cells into the adjacent PV, CV, BD, and SS. According to the tumor doubling time, the mean diameter
of the macrometastases in 19 remnant livers was calculated to have been 0.57±0.87 cm at the time of the primary resection.
The calculated diameter of 3 of these 19 macrometastases was found to be less than 0.01 cm, the minimum implantable size,
indicating that the cancer recurrence in these specimens may have developed from macroscopic metastatic lesions as a satellite,
and not from the primary tumor. In 13 patients who received doses of 5250 mg or more of 5 fluorouracil (FU) via the hepatic
artery, the cumulative disease-free rate 2 years postoperatively was 100%; this value was 47.6% in 11 patients who received
less than 5250 mg of 5 FU via the hepatic artery, and 0% in 39 patients who received no chemotherapy (P<0.005). These results suggest that anatomical hepatic resection for satellite lesions, combined with prophylactic hepatic
arterial chemotherapy for micrometastases, decreases the recurrence rate of hepatic metastases in the remnant liver. 相似文献
100.
PHILIPPE KIRSTETTER CHRISTOPHE PILETTE RICHARD MOREAU STEPHANE CAILMAIL VACLAV SAFKA THIERRY SOUPISON DIDIER LEBREC 《Journal of gastroenterology and hepatology》1996,11(3):230-235
The haemodynamic effects of nitrovasodilators and their mechanisms of action on portal hypertension remain unclear. The splanchnic and systemic haemodynamic response to the infusion of isosorbide dinitrate (100 μg/kg per min), a nitrovasodilator, was investigated in cirrhotic rats. The role of the conscious state in the haemodynamic response to isosorbide dinitrate was examined using rats that were anaesthetized with pentobarbitone. The role of sympathetic tone in the haemodynamic response to isosorbide dinitrate was examined using rats pretreated with the ganglion blocker hexamethonium. Isosorbide dinitrate had no haemodynamic effects in conscious, unblocked normal and cirrhotic rats. Isosorbide dinitrate had no haemodynamic effects in normal and cirrhotic rats treated with hexamethonium. In normal anaesthetized rats, isosorbide dinitrate significantly decreased systemic vascular resistance (414±25 vs 290±26 dyn.s/cm5 per 100 g). In cirrhotic anaesthetized rats, isosorbide dinitrate significantly decreased mean arterial pressure (98±6 vs 79±7 mmHg), systemic vascular resistance (318±30 vs 207±10 dyn.s/cm5 per 100 g), portal pressure (14.0±1.0 vs 11.3±0.9 mmHg) and portal territory vascular resistance (1362±163 vs 1031±182 dyn.s/cm5 per 100 g). In conclusion, this study shows that the portal hypotensive effects of isosorbide dinitrate depend upon the alterations of vascular tone by pentobarbitone. 相似文献