正交设计法探讨生姜中总黄酮的提取方案

目的 采用正交设计法确定生姜中总黄酮的最佳提取方案. 方法 以不同浓度的乙醇进行单因素实验,选择出最佳提取溶剂.再以回流次数、回流时间、所用溶剂体积3个因素,采用3×3正交设计法对生姜中总黄酮进行回流提取,并以紫外可见分光光度计对生姜中总黄酮进行含量测定. 结果 最佳提取方案为:药材与提取溶剂的用量比1:10、回流提取3次、每次1.5 h. 结论 该提取方法 操作简便,结果 准确可靠,为进一步研究生姜中总黄酮提取工艺提供理论依据.     

督灸姜泥墩厚度与艾绒直径高度最佳组合研究

目的 观察不同姜泥墩厚度、艾绒直径高度组合在督灸时的灸温及持续时间,为临床操作提供参考。方法 将入选的300名志愿者随机分成10组,每组30人,分别在不同厚度的姜泥墩上采用不同直径高度的艾绒实施督灸,用数字温度计持续测温,记录每组灸温达到43℃时间、维持43~45℃的时间以及志愿者疼痛程度。结果 不同姜泥墩厚度、艾绒直径高度组合中6组能达到43℃灸温,且能维持43~45℃一段时间。组间比较,差异有统计学意义（P<0.01,P<0.05）。姜泥墩厚度为2.5 cm、艾绒直径和高度为2.0 cm督灸组不仅能在（38.24±3.19）min达到43℃的灸温、并能维持43~45℃灸温（21.76±3.06）min,疼痛评分为（3.40±0.71）分。结论 姜泥墩厚度为2.5 cm,艾绒直径和高度为2.0 cm为最佳督灸组合,该组不仅能达到43℃,维持43~45℃时间最长,且志愿者疼痛较轻微。     

Protective effect of mirtazapine versus ginger against cisplatin-induced testicular damage in adult male albino rats

Cisplatin (CP) is a chemotherapy medication used to treat different types of organs cancers. It has damaging effects on testes. Mirtazapine is an antidepressant, which is used primarily in the treatment of depression and other anxiety disorders. Ginger is a naturally growing plant with antioxidant properties. Thirty-six adult male albino rats, subdivided into six groups (six animals each) received treatment for 30 days. Group I (control) received saline solution orally; group II received mirtazapine (20 mg/kg). Group III received ginger (200 mg/kg/day), group IV received CP (7 mg/kg) IP single dose, at day 23rd, group V received mirtazapine (200 mg/day) orally till day 23rd, CP (7 mg/kg) IP at day 23rd, mirtazapine till day 30th, group VI received ginger (200 mg/Kg/day) orally till day 23rd, CP (7 mg/kg) IP at day 23rd, and then ginger at the previous dose till day 30th. This study examined the microscopic changes associated with CP and the possible testicular protective role of mirtazapine versus ginger of adult male rats. Mirtazapine and ginger resulted in cellular protection of testicular tissue as evident from microscopic changes including Sertoli cells, spermatogonia, and Leydig cells. Ginger showed to have a more protective effect than mirtazapine on testicular tissue against CP treatment.     

Ginger for Prevention of Antituberculosis‐induced Gastrointestinal Adverse Reactions Including Hepatotoxicity: A Randomized Pilot Clinical Trial

In this study, the potential benefits of ginger in preventing antituberculosis drug‐induced gastrointestinal adverse reactions including hepatotoxicity have been evaluated in patients with tuberculosis. Patients in the ginger and placebo groups (30 patients in each group) received either 500 mg ginger (Zintoma)^® or placebo one‐half hour before each daily dose of antituberculosis drugs for 4 weeks. Patients' gastrointestinal complaints (nausea, vomiting, dyspepsia, and abdominal pain) and antituberculosis drug‐induced hepatotoxicity were recorded during the study period. In this cohort, nausea was the most common antituberculosis drug‐induced gastrointestinal adverse reactions. Forty eight (80%) patients experienced nausea. Nausea was more common in the placebo than the ginger group [27 (90%) vs 21 (70%), respectively, p = 0.05]. During the study period, 16 (26.7%) patients experienced antituberculosis drug‐induced hepatotoxicity. Patients in the ginger group experienced less, but not statistically significant, antituberculosis drug‐induced hepatotoxicity than the placebo group (16.7% vs 36.7%, respectively, p = 0.07). In conclusion, ginger may be a potential option for prevention of antituberculosis drug‐induced gastrointestinal adverse reactions including hepatotoxicity. Copyright © 2016 John Wiley & Sons, Ltd.     

Plant-derived exosomal microRNAs inhibit lung inflammation induced by exosomes SARS-CoV-2 Nsp12

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子午流注法择时施灸治疗老年夜间尿频的临床效果

目的 探讨子午流注法择时施灸治疗老年夜间尿频的临床效果.方法 选取2017年3月至2018年3月在我院脑病二科住院的60例老年夜间尿频患者,通过随机数字表法将其分为治疗组[30例,17:00～19:00(酉时)行隔姜灸治疗]和对照组[30例,9:00～11:00(巳时)行隔姜灸治疗].比较两组患者治疗前、后的夜尿次数、...     

陈皮干姜油对疲劳大鼠血液生化、血气分析及肌糖元的影响

目的:研究陈皮干姜油对疲劳大鼠血液生化、血气分析及肌糖元的影响。方法:SD大鼠随机分为4组,连续给药15天后造成运动疲劳模型,分别进行血液生化、血气分析及肌糖元测定。结果:陈皮干姜油可升高运动疲劳大鼠的白蛋白、碱性磷酸酶、血氧饱和度及氧含量,降低总胆红素含量,并有降低疲劳大鼠运动后血清肌酸激酶水平、升高氧分压及肌糖元的趋势。结论:陈皮干姜油可部分改善疲劳大鼠血液生化、血气分析指标,促进运动后疲劳恢复。     

厚朴姜制前后所含厚朴酚及和厚朴酚总量的变化

目的：临床使用厚朴均为姜制品,但药典仅有厚朴原药材的含量测定而无厚朴姜制品含量测定的规定,给厚朴饮片的质量控制带来难度,本试验通过检验厚朴姜制前后所含厚朴酚及和厚朴酚总量的变化并参考药典对厚朴原药材的含量限度。为制订姜制品的含量限度提供依据。方法：本文参照中国药典2000年版一部厚朴项下含量测定法（HPLC法）对不同产地、同批不同株及同株等数种情况九批厚朴姜制前后所含厚朴酚及和厚朴酚进行含量测定。结果：厚朴姜制前后所含厚朴酚及和厚朴酚总量降低13-15%。结论：根据所得实验结果并参考药典对厚朴药材含测的规定,对姜制厚朴厚朴酚及和厚朴酚含量应不低于1.6%为宜。     

姜半夏渗漉工艺研究

目的：研究姜半夏最佳提取工艺条件。方法：采用3因素3水平L9（3^4)正交试验法,以总生物碱含量作为评判指标,对姜半夏渗漉工艺进行研究。结果：实验设计3个因素中乙醇浓度因素具有极显著性影响。结论：结合实际生产情况,姜半夏总生物碱提取的最佳工艺条件为A3B1C1。     

《伤寒论》方中生姜的应用

分析《伤寒论》方中生姜的应用,认为其具有调和营卫、温阳散水、辛散宣通、和胃止呕等功效。