幽门螺杆菌感染对胃黏膜三叶因子2表达的影响

目的探讨幽门螺杆菌感染(Hp)对慢性胃炎胃黏膜三叶因子2(TFF2)表达的影响。方法采用免疫组化及半定量的分析方法检测Hp阳性组与Hp阴性组,以及Hp根除前后胃窦黏膜TFF2表达量的变化。结果TFF2阳性物质表达于胃黏膜上皮近基底部的胃腺细胞的细胞质内,呈棕黄色颗粒;与Hp阴性组比较,Hp阳性组的TFF2染色评分显著减少(P<0.05);根除Hp后的胃黏膜较根除前TFF2的表达量显著增加(P<0.05)。结论Hp感染导致胃黏膜TFF2表达减少,提示TFF2在Hp相关性胃炎的发病机制中起一定作用。     

军演应激状态对官兵部分生理生化指标的影响

目的 探讨军事演习应激状态下官兵部分生理生化指标的变化.方法 选择陆军官兵133人为研究对象,分别在军演前1天和军演结束后1天用酶联免疫吸附法(ELISA)测定血清可溶性肿瘤坏死因子受体-1(sTNFR-1)和血清可溶性细胞间黏附分子-1(sICAM-1)水平,放射免疫法(RIA)测定血清胃泌素(G)和睾酮(T)水平,生化法检测血清超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,并探讨这些指标变化与心理应激的关系.结果 参加军演后的官兵血清sTNFR-1、sICAM-1、T、MDA水平均升高,G、SOD水平均降低,与军演前比较有显著性差异(P＜0.05);与70名曾参加过军演的官兵比较,63名未参加过军演者的血清sTNFR-1、sICAM-1、MDA水平升高更为明显(P＜0.05),血清SOD水平降低亦更为明显(P＜0.05),而G、T的变化则无明显差异(P＞0.05).结论 军演时官兵处于较强的生理应激状态,导致机体在免疫、抗氧化能力、胃肠激素、性激素等方面出现一系列变化;加强平时军事训练可以提高部队指战员应对军事应激的能力.     

血清胃蛋白酶原以及胃泌素-17与慢性胃炎

目的:探讨胃蛋白酶原(PG)和胃泌素-17(G-17)水平与慢性胃炎的关系.方法:30例SD大鼠于随机分为两组,实验组进行慢性胃炎动物模型复制,对照组正常进食进饮;造模结束后,以酶联免疫测定法检测两组大鼠血清PG I、PGⅡ及PGI/PGⅡ比值(PGR)和G-17水平.结果:慢性胃炎组与对照组相比,血清PG I水平和P...     

Ascorbic acid secretion in the human stomach and the effect of gastrin

AIM:To investigate the changes of gastric mucosal ascorbic acid secretion in patients with nonulcer dyspepsia and the effect of gastrin on it, and to relate any observed changes to H.pylori infection and mucosal histology.METHODS:Ascorbic acid secretions in patients were examined by collecting continuously gastric juice for one hour after having aspirated and discarded fasting gastric juice. Using the clearance rate (mL/min) of ascorbic acid from blood to gastric juice represented ascorbic acid secretion in the gastric mucosa.Ascorbic acid concentrations in plasma and juice were measured by ferric reduced method.RESULTS:Gastric ascorbic acid secretions in H.pylori-positive patients (1.46mL/min,range 0.27-3.78) did not significantly differ from those in H.pylori-negative patients(1.25mL/min, 0.47-3.14)(P>0.05). There were no significant differences in ascorbic acid secretions between patients with mild (1.56mL/min, 0.50-3.30), moderate (1.34mL/min, 0.27-2.93) and severe (1.36mL/min, 0.47-3.78) inflammation (P >0.05). There were no significant differences in ascorbic acid secretions between patients without activity (1.45mL/min, 0.27-3.14) and with mild (1.32mL/min, 0.61-2.93), moderate (1.49mL/min, 0.50-3.78) and severe (1.43mL/min, 0.51-3.26) activity of chronic gastritis either (P>0.05). Ascorbic acid secretions in patients with severe atrophy (0.56mL/min, 0.27-1.20) were markedly lower than those in patients without atrophy (1.51mL/min, 0.59-3.30) and with mild (1.43mL/min, 0.53-3.78) and moderate (1.31mL/min,0.47-3.16)atrophy(P<0.005). There was a significant negative correlation between ascorbic acid secretion and severity of atrophy (correlation coefficient =-0.43, P<0.005). After administration of pentagastrin, ascorbic acid secretions were markedly elevated (from 1.39mL/min, 0.36-2.96 to 3.53mL/min, 0.84-5.91) (P <0.001).CONCLUSION:Ascorbic acid secretion in gastric mucosa is not affected by H.pylori infection. Gastric ascorbic acid secretion is markedly related to the severity of atrophy, whereas not related to the severity of inflammation and activity. Gastrin may stimulate gastric ascorbic acid secretion. A decreased ascorbic acid secretion may be an important factor in the link between atrophic gastritis and gastric carcinogenesis.     

胃肠激素在早期肠内营养预防应激性溃疡的表达研究

目的 探讨胃肠激素在早期肠内营养预防应激性溃疡的作用.方法 将38例危重患者随机分为3组:A组13例,给予抑酸药物加早期(发病24 h内)肠内营养;B组13例,给予抑酸药物加延迟(发病72 h后)肠内营养;C组12例,单纯应用抑酸药物.于发病后5 d、2周时分别行胃镜检查,观察溃疡的发生情况;同时测定各组前列腺素(PGE-2)和胃泌素(GAS)、生长抑素(SS)的含量.结果 A组溃疡发生率及溃疡指数均明显低于B、C组(P<0.01),溃疡抑制率高于其他两组(P<0.01);A、B组2周后溃疡愈合率高于C组(P<0.05).与B、C组比较,A组胃黏膜中的PGE-2、GAS水平明显增高,SS水平明显降低(P<0.05);B、C组间亦有统计学差异(P<0.05).结论 应用抑酸药物同时给予早期肠内营养治疗可能对应激性溃疡的发生有一定的预防作用.肠内营养可能通过调节胃肠激素含量起作用.     

大肠癌患者手术前后血清胃泌素含量研究

应用放射免疫分析法测定了４３例大肠癌患者手术前后和２０例对照组空腹血清胃泌素（ＳＧ）水平。结果表明：大肠癌患者ＳＧ显著高于对照组（Ｐ〈０．０１）；Ｄｕｋｅｓ Ｃ、Ｄ期大肠癌患者ＳＧ显著高于Ｄｕｋｅｓ Ａ、Ｂ期（Ｐ〈０．０５）；不同肿瘤组织学类型之间ＳＧ差异无显著性；根治性大肠癌切除术后ＳＧ较术前显著降低（Ｐ〈０．０１）；姑息性手术前后ＳＧ差异无显著性。提示ＳＧ水平与大肠癌生长密切相关，ＳＧ测定有助     

窒息新生儿血中胃泌素及胃动素的变化

目的: 探讨窒息新生儿血中胃泌素(gastrin,GAS)和胃动素(motilin,MOT) 的变化及其与胃肠道功能紊乱间的关系.方法: 放射免疫法检测窒息新生儿生后1,3,7 d空腹静脉血中GAS,MOT水平,并设对照组.结果: 窒息新生儿第1天血GAS,MOT明显高于对照组,差异有统计学意义(P＜0.05);第3,7 d GAS基本接近对照组,差异无统计学意义(P＞0.05),MOT低于对照组,但无统计学差异(P＞0.05).窒息组18例患儿中有10例发生不同程度的胃肠道功能紊乱,其第1,3天血GAS分别为(335.51±67.41) ng/L和(287.98±58.06) ng/L,与8例未发生胃肠道功能紊乱患儿的(265.23±42.31) ng/L,(234.01±47.11) ng/L相比较明显升高,其中以第1天尤为显著,有统计学意义(P＜0.05);第1,3天血中MOT水平分别为(182.71±73.37) ng/L和(143.75±46.79) ng/L,均低于未发生胃肠功能障碍患儿的(215.37±79.69) ng/L和(170.08±48.14) ng/L,但尚无统计学意义(P＞0.05). 结论: 窒息新生儿GAS和MOT分泌异常可能与胃肠道功能紊乱有关.     

胃泌素及神经酰胺与大肠肿瘤的关系

现已证实,部分大肠肿瘤细胞及组织中胃泌素异常表达,胃泌素的异常表达与大肠肿瘤的发生、发展关系密切.胃泌素与受体结合后可通过一系列网络信号转导途径,使神经酰胺含量减少,促进肿瘤细胞的增生.阻断此途径,可能为大肠肿瘤的预防及治疗提供一个新的切人点.     

黄酮甙对大鼠胃泌素及胃运动的影响

目的研究黄酮甙对胃泌素及胃运动的影响。方法实验组(A组)从枳实中提取黄酮甙对大鼠灌胃,B组用红霉素对大鼠灌胃,C组用生理盐水对大鼠灌胃。检测各组大鼠血浆中胃泌素含量,用生理记录仪记录黄酮甙对胃排空和胃运动的影响。结果 A、B组与C组比较,大鼠血浆中胃泌素含量均明显增高(P<0.05);A组胃泌素含量明显高于B组(P<0.05)。A、B组胃肌电慢波频率与C组比较,差异有统计学意义(P<0.01);A组与B组比较,差异也有统计学意义(P<0.05)。结论黄酮甙通过促进胃泌素的分泌提高胃的收缩力,增强胃排空来调节胃运动。